@conference{
author = "Davidović, Slađana and Miljković, Miona and Ilić, Nevena and Milošević, Jelena and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023",
title = "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7030"
}
