TY  - JOUR
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Beluhan, Sunčica
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6374
AB  - Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.
PB  - MDPI
T2  - Energies
T1  - Cellulases: From Lignocellulosic Biomass to Improved Production
IS  - 8
SP  - 3598
VL  - 16
DO  - 10.3390/en16083598
ER  - 

@article{
author = "Ilić, Nevena and Milić, Marija and Beluhan, Sunčica and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.",
publisher = "MDPI",
journal = "Energies",
title = "Cellulases: From Lignocellulosic Biomass to Improved Production",
number = "8",
pages = "3598",
volume = "16",
doi = "10.3390/en16083598"
}

Ilić, N., Milić, M., Beluhan, S.,& Dimitrijević-Branković, S.. (2023). Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies
MDPI., 16(8), 3598.
https://doi.org/10.3390/en16083598

Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies. 2023;16(8):3598.
doi:10.3390/en16083598 .

Ilić, Nevena, Milić, Marija, Beluhan, Sunčica, Dimitrijević-Branković, Suzana, "Cellulases: From Lignocellulosic Biomass to Improved Production" in Energies, 16, no. 8 (2023):3598,
https://doi.org/10.3390/en16083598 . .

TY  - JOUR
AU  - Beluhan, Sunčica
AU  - Mihajlovski, Katarina
AU  - Šantek, Božidar
AU  - Ivančić Šantek, Mirela
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6728
AB  - Bioethanol is the most widely used alternative transportation fuel to petrol. Bioethanol is considered a clean, renewable, and environmentally friendly fuel that can contribute to climate change mitigation, decreased environmental pollution, and enhanced energy security. Commercial bioethanol production is based on traditional agricultural crops such as corn, sugarcane, and sugarbeet, primarily used as food and feed. In order to meet the growing demand for this fuel and decrease competition in the food and biofuel sectors for the same feedstock, other raw materials and process technologies have been intensively studied. Lignocellulosic biomass is one of the most abundant renewable resources, with it being rich in compounds that could be processed into energy, transportation fuels, various chemical compounds, and diverse materials. Bioethanol production from lignocellulosic biomass has received substantial attention in recent decades. This review gives an overview of bioethanol production steps from lignocellulosic biomass and challenges in the production process. The following aspects of bioethanol production are covered here, including pretreatment methods, process strategies, strain development, ethanol isolation and purification, and technical hurdles.
PB  - MDPI
T2  - Energies
T1  - The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing
IS  - 19
SP  - 7003
VL  - 16
DO  - 10.3390/en16197003
ER  - 

@article{
author = "Beluhan, Sunčica and Mihajlovski, Katarina and Šantek, Božidar and Ivančić Šantek, Mirela",
year = "2023",
abstract = "Bioethanol is the most widely used alternative transportation fuel to petrol. Bioethanol is considered a clean, renewable, and environmentally friendly fuel that can contribute to climate change mitigation, decreased environmental pollution, and enhanced energy security. Commercial bioethanol production is based on traditional agricultural crops such as corn, sugarcane, and sugarbeet, primarily used as food and feed. In order to meet the growing demand for this fuel and decrease competition in the food and biofuel sectors for the same feedstock, other raw materials and process technologies have been intensively studied. Lignocellulosic biomass is one of the most abundant renewable resources, with it being rich in compounds that could be processed into energy, transportation fuels, various chemical compounds, and diverse materials. Bioethanol production from lignocellulosic biomass has received substantial attention in recent decades. This review gives an overview of bioethanol production steps from lignocellulosic biomass and challenges in the production process. The following aspects of bioethanol production are covered here, including pretreatment methods, process strategies, strain development, ethanol isolation and purification, and technical hurdles.",
publisher = "MDPI",
journal = "Energies",
title = "The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing",
number = "19",
pages = "7003",
volume = "16",
doi = "10.3390/en16197003"
}

Beluhan, S., Mihajlovski, K., Šantek, B.,& Ivančić Šantek, M.. (2023). The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing. in Energies
MDPI., 16(19), 7003.
https://doi.org/10.3390/en16197003

Beluhan S, Mihajlovski K, Šantek B, Ivančić Šantek M. The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing. in Energies. 2023;16(19):7003.
doi:10.3390/en16197003 .

Beluhan, Sunčica, Mihajlovski, Katarina, Šantek, Božidar, Ivančić Šantek, Mirela, "The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing" in Energies, 16, no. 19 (2023):7003,
https://doi.org/10.3390/en16197003 . .

TY  - JOUR
AU  - Grubišić, Marina
AU  - Perečinec Galić, Maja
AU  - Peremin, Ines
AU  - Mihajlovski, Katarina
AU  - Beluhan, Sunčica
AU  - Šantek, Božidar
AU  - Šantek Ivančić, Mirela
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5128
AB  - Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.
PB  - MDPI
T2  - Energies
T1  - Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus
IS  - 9
SP  - 3208
VL  - 15
DO  - 10.3390/en15093208
ER  - 

@article{
author = "Grubišić, Marina and Perečinec Galić, Maja and Peremin, Ines and Mihajlovski, Katarina and Beluhan, Sunčica and Šantek, Božidar and Šantek Ivančić, Mirela",
year = "2022",
abstract = "Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.",
publisher = "MDPI",
journal = "Energies",
title = "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus",
number = "9",
pages = "3208",
volume = "15",
doi = "10.3390/en15093208"
}

Grubišić, M., Perečinec Galić, M., Peremin, I., Mihajlovski, K., Beluhan, S., Šantek, B.,& Šantek Ivančić, M.. (2022). Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies
MDPI., 15(9), 3208.
https://doi.org/10.3390/en15093208

Grubišić M, Perečinec Galić M, Peremin I, Mihajlovski K, Beluhan S, Šantek B, Šantek Ivančić M. Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies. 2022;15(9):3208.
doi:10.3390/en15093208 .

Grubišić, Marina, Perečinec Galić, Maja, Peremin, Ines, Mihajlovski, Katarina, Beluhan, Sunčica, Šantek, Božidar, Šantek Ivančić, Mirela, "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus" in Energies, 15, no. 9 (2022):3208,
https://doi.org/10.3390/en15093208 . .