TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gökhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana B.
AU  - Milinčić, Danijel D.
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7457
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
SP  - 518
VL  - 17
DO  - 10.3390/ph17040518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gökhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana B. and Milinčić, Danijel D. and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
pages = "518",
volume = "17",
doi = "10.3390/ph17040518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M. B., Milinčić, D. D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić MB, Milinčić DD, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gökhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana B., Milinčić, Danijel D., Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Belošević, Spasoje D.
AU  - Milinčić, Danijel D.
AU  - Gašić, Uroš M.
AU  - Kostić, Aleksandar Ž.
AU  - Salević-Jelić, Ana S.
AU  - Marković, Jovana M.
AU  - Đorđević, Verica B.
AU  - Lević, Steva M.
AU  - Pešić, Mirjana B.
AU  - Nedović, Viktor A.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7383
AB  - The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.
PB  - MDPI
T2  - Foods
T1  - Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties
IS  - 5
SP  - 757
VL  - 13
DO  - 10.3390/foods13050757
ER  - 

@article{
author = "Belošević, Spasoje D. and Milinčić, Danijel D. and Gašić, Uroš M. and Kostić, Aleksandar Ž. and Salević-Jelić, Ana S. and Marković, Jovana M. and Đorđević, Verica B. and Lević, Steva M. and Pešić, Mirjana B. and Nedović, Viktor A.",
year = "2024",
abstract = "The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.",
publisher = "MDPI",
journal = "Foods",
title = "Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties",
number = "5",
pages = "757",
volume = "13",
doi = "10.3390/foods13050757"
}

Belošević, S. D., Milinčić, D. D., Gašić, U. M., Kostić, A. Ž., Salević-Jelić, A. S., Marković, J. M., Đorđević, V. B., Lević, S. M., Pešić, M. B.,& Nedović, V. A.. (2024). Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties. in Foods
MDPI., 13(5), 757.
https://doi.org/10.3390/foods13050757

Belošević SD, Milinčić DD, Gašić UM, Kostić AŽ, Salević-Jelić AS, Marković JM, Đorđević VB, Lević SM, Pešić MB, Nedović VA. Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties. in Foods. 2024;13(5):757.
doi:10.3390/foods13050757 .

Belošević, Spasoje D., Milinčić, Danijel D., Gašić, Uroš M., Kostić, Aleksandar Ž., Salević-Jelić, Ana S., Marković, Jovana M., Đorđević, Verica B., Lević, Steva M., Pešić, Mirjana B., Nedović, Viktor A., "Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties" in Foods, 13, no. 5 (2024):757,
https://doi.org/10.3390/foods13050757 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Miladinović, Marija
AU  - Gašić, Uroš
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić‑Stojanović, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5137
AB  - Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Biomass Conversion and Biorefinery
T1  - Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction
DO  - 10.1007/s13399-022-02717-5
ER  - 

@article{
author = "Milutinović, Milica and Miladinović, Marija and Gašić, Uroš and Dimitrijević-Branković, Suzana and Rajilić‑Stojanović, Mirjana",
year = "2022",
abstract = "Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Biomass Conversion and Biorefinery",
title = "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction",
doi = "10.1007/s13399-022-02717-5"
}

Milutinović, M., Miladinović, M., Gašić, U., Dimitrijević-Branković, S.,& Rajilić‑Stojanović, M.. (2022). Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s13399-022-02717-5

Milutinović M, Miladinović M, Gašić U, Dimitrijević-Branković S, Rajilić‑Stojanović M. Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery. 2022;.
doi:10.1007/s13399-022-02717-5 .

Milutinović, Milica, Miladinović, Marija, Gašić, Uroš, Dimitrijević-Branković, Suzana, Rajilić‑Stojanović, Mirjana, "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction" in Biomass Conversion and Biorefinery (2022),
https://doi.org/10.1007/s13399-022-02717-5 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5298
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
SP  - 1537
VL  - 15
DO  - 10.3390/ph15121537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
pages = "1537",
volume = "15",
doi = "10.3390/ph15121537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .