TY  - JOUR
AU  - Milivojević, Marija
AU  - Chen, Ke
AU  - Radovanović, Željko
AU  - Petrović, Rada
AU  - Dimitrijević-Branković, Suzana
AU  - Kojić, Vesna
AU  - Marković, Danica
AU  - Janaćković, Đorđe
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6803
AB  - The restoration of large bone defects caused by trauma, tumor resection, or infection is a major clinical problem in orthopedics and dentistry because postoperative infections, corrosion, and limited osteointegration of metal implants can lead to loosening of the implant. The aim of this study was to improve the surface properties of a 3D-printed (electron beam melting) Ti6Al4V-based macroporous scaffold by multilayer coating with bioactive silicate glasses (BAGs) and hydroxyapatite doped with a silver (AgHAP) or AgHAP additionally sonochemically modified with ZnO (ZnO-AgHAP). The coated scaffolds AgHAP_BAGs_Ti and ZnO-AgHAP_BAGs_Ti enhanced cytocompatibility in L929 and MRC5 cell lines and expressed bioactivity in simulated body fluid. A lower release of vanadium ions in coated samples compared to bare Ti scaffold indicates decreased dissolution of Ti alloy in coated samples. The coated samples reduced growth ofEscherichia coliandStaphylococcus aureusfor 4-6 orders of magnitude. Therefore, the 3D-printed Ti-based scaffolds coated with BAGs and (ZnO-)AgHAP have great potential for application as a multifunctional implant with antibacterial properties for the restoration of defects in load-bearing bones.
PB  - IOP Publishing
T2  - Biomedical Materials
T1  - Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects
IS  - 6
SP  - 065020
VL  - 18
DO  - 10.1088/1748-605X/ad02d2
ER  - 

@article{
author = "Milivojević, Marija and Chen, Ke and Radovanović, Željko and Petrović, Rada and Dimitrijević-Branković, Suzana and Kojić, Vesna and Marković, Danica and Janaćković, Đorđe",
year = "2023",
abstract = "The restoration of large bone defects caused by trauma, tumor resection, or infection is a major clinical problem in orthopedics and dentistry because postoperative infections, corrosion, and limited osteointegration of metal implants can lead to loosening of the implant. The aim of this study was to improve the surface properties of a 3D-printed (electron beam melting) Ti6Al4V-based macroporous scaffold by multilayer coating with bioactive silicate glasses (BAGs) and hydroxyapatite doped with a silver (AgHAP) or AgHAP additionally sonochemically modified with ZnO (ZnO-AgHAP). The coated scaffolds AgHAP_BAGs_Ti and ZnO-AgHAP_BAGs_Ti enhanced cytocompatibility in L929 and MRC5 cell lines and expressed bioactivity in simulated body fluid. A lower release of vanadium ions in coated samples compared to bare Ti scaffold indicates decreased dissolution of Ti alloy in coated samples. The coated samples reduced growth ofEscherichia coliandStaphylococcus aureusfor 4-6 orders of magnitude. Therefore, the 3D-printed Ti-based scaffolds coated with BAGs and (ZnO-)AgHAP have great potential for application as a multifunctional implant with antibacterial properties for the restoration of defects in load-bearing bones.",
publisher = "IOP Publishing",
journal = "Biomedical Materials",
title = "Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects",
number = "6",
pages = "065020",
volume = "18",
doi = "10.1088/1748-605X/ad02d2"
}

Milivojević, M., Chen, K., Radovanović, Ž., Petrović, R., Dimitrijević-Branković, S., Kojić, V., Marković, D.,& Janaćković, Đ.. (2023). Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects. in Biomedical Materials
IOP Publishing., 18(6), 065020.
https://doi.org/10.1088/1748-605X/ad02d2

Milivojević M, Chen K, Radovanović Ž, Petrović R, Dimitrijević-Branković S, Kojić V, Marković D, Janaćković Đ. Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects. in Biomedical Materials. 2023;18(6):065020.
doi:10.1088/1748-605X/ad02d2 .

Milivojević, Marija, Chen, Ke, Radovanović, Željko, Petrović, Rada, Dimitrijević-Branković, Suzana, Kojić, Vesna, Marković, Danica, Janaćković, Đorđe, "Enhanced antimicrobial properties and bioactivity of 3D-printed titanium scaffolds by multilayer bioceramic coating for large bone defects" in Biomedical Materials, 18, no. 6 (2023):065020,
https://doi.org/10.1088/1748-605X/ad02d2 . .

TY  - CONF
AU  - Nešović, Katarina
AU  - Janković, Ana
AU  - Surudžić, Rade
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Radetić, Tamara
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6761
AB  - Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.
PB  - Mary Ann Liebert, Inc.
C3  - Tissue Engineering Part A
T1  - Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis
IS  - S1
SP  - S-132
VL  - 28
DO  - 10.1089/ten.tea.2022.29025.abstracts
ER  - 

@conference{
author = "Nešović, Katarina and Janković, Ana and Surudžić, Rade and Vukašinović-Sekulić, Maja and Kojić, Vesna and Radetić, Tamara and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37  C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.",
publisher = "Mary Ann Liebert, Inc.",
journal = "Tissue Engineering Part A",
title = "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis",
number = "S1",
pages = "S-132",
volume = "28",
doi = "10.1089/ten.tea.2022.29025.abstracts"
}

Nešović, K., Janković, A., Surudžić, R., Vukašinović-Sekulić, M., Kojić, V., Radetić, T.,& Mišković-Stanković, V.. (2022). Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A
Mary Ann Liebert, Inc.., 28(S1), S-132.
https://doi.org/10.1089/ten.tea.2022.29025.abstracts

Nešović K, Janković A, Surudžić R, Vukašinović-Sekulić M, Kojić V, Radetić T, Mišković-Stanković V. Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis. in Tissue Engineering Part A. 2022;28(S1):S-132.
doi:10.1089/ten.tea.2022.29025.abstracts .

Nešović, Katarina, Janković, Ana, Surudžić, Rade, Vukašinović-Sekulić, Maja, Kojić, Vesna, Radetić, Tamara, Mišković-Stanković, Vesna, "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis" in Tissue Engineering Part A, 28, no. S1 (2022):S-132,
https://doi.org/10.1089/ten.tea.2022.29025.abstracts . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4839
AB  - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
PB  - Elsevier B.V.
T2  - Journal of Materials Research and Technology
T1  - The chitosan-based bioactive composite coating on titanium
EP  - 4474
SP  - 4461
VL  - 15
DO  - 10.1016/j.jmrt.2021.10.072
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2021",
abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.",
publisher = "Elsevier B.V.",
journal = "Journal of Materials Research and Technology",
title = "The chitosan-based bioactive composite coating on titanium",
pages = "4474-4461",
volume = "15",
doi = "10.1016/j.jmrt.2021.10.072"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology
Elsevier B.V.., 15, 4461-4474.
https://doi.org/10.1016/j.jmrt.2021.10.072

Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology. 2021;15:4461-4474.
doi:10.1016/j.jmrt.2021.10.072 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology, 15 (2021):4461-4474,
https://doi.org/10.1016/j.jmrt.2021.10.072 . .