TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Lađarević, Jelena
AU  - Onjia, Antonije
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444
AB  - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
DO  - 10.1007/s11356-024-33367-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}

Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-024-33367-x

Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;.
doi:10.1007/s11356-024-33367-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024),
https://doi.org/10.1007/s11356-024-33367-x . .

TY  - CHAP
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Milić, Marija
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7427
AB  - Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.
PB  - Cham : Springer
T2  - Biorefinery and Industry 4.0: Empowering Sustainability
T1  - The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production
EP  - 179
SP  - 113
DO  - 10.1007/978-3-031-51601-6_5
ER  - 

@inbook{
author = "Mihajlovski, Katarina and Ilić, Nevena and Milić, Marija",
year = "2024",
abstract = "Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.",
publisher = "Cham : Springer",
journal = "Biorefinery and Industry 4.0: Empowering Sustainability",
booktitle = "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production",
pages = "179-113",
doi = "10.1007/978-3-031-51601-6_5"
}

Mihajlovski, K., Ilić, N.,& Milić, M.. (2024). The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability
Cham : Springer., 113-179.
https://doi.org/10.1007/978-3-031-51601-6_5

Mihajlovski K, Ilić N, Milić M. The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability. 2024;:113-179.
doi:10.1007/978-3-031-51601-6_5 .

Mihajlovski, Katarina, Ilić, Nevena, Milić, Marija, "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production" in Biorefinery and Industry 4.0: Empowering Sustainability (2024):113-179,
https://doi.org/10.1007/978-3-031-51601-6_5 . .

TY  - JOUR
AU  - Milić, Marija
AU  - Buntić, Aneta V.
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981
AB  - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
T2  - Biomass Conversion and Biorefinery
T1  - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
EP  - 6759
IS  - 8
SP  - 6747
VL  - 13
DO  - 10.1007/s13399-021-01605-8
ER  - 

@article{
author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.",
journal = "Biomass Conversion and Biorefinery",
title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization",
pages = "6759-6747",
number = "8",
volume = "13",
doi = "10.1007/s13399-021-01605-8"
}

Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759.
https://doi.org/10.1007/s13399-021-01605-8

Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759.
doi:10.1007/s13399-021-01605-8 .

Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759,
https://doi.org/10.1007/s13399-021-01605-8 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Beluhan, Sunčica
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6374
AB  - Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.
PB  - MDPI
T2  - Energies
T1  - Cellulases: From Lignocellulosic Biomass to Improved Production
IS  - 8
SP  - 3598
VL  - 16
DO  - 10.3390/en16083598
ER  - 

@article{
author = "Ilić, Nevena and Milić, Marija and Beluhan, Sunčica and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.",
publisher = "MDPI",
journal = "Energies",
title = "Cellulases: From Lignocellulosic Biomass to Improved Production",
number = "8",
pages = "3598",
volume = "16",
doi = "10.3390/en16083598"
}

Ilić, N., Milić, M., Beluhan, S.,& Dimitrijević-Branković, S.. (2023). Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies
MDPI., 16(8), 3598.
https://doi.org/10.3390/en16083598

Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies. 2023;16(8):3598.
doi:10.3390/en16083598 .

Ilić, Nevena, Milić, Marija, Beluhan, Sunčica, Dimitrijević-Branković, Suzana, "Cellulases: From Lignocellulosic Biomass to Improved Production" in Energies, 16, no. 8 (2023):3598,
https://doi.org/10.3390/en16083598 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6605
AB  - Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
T1  - APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET
EP  - 186
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6605
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23",
title = "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET",
pages = "186-179",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6605"
}

Ilić, N., Milić, M., Davidović, S., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
Zvornik : University of East Sarajevo Faculty of Technology., 179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605

Ilić N, Milić M, Davidović S, Dimitrijević-Branković S, Mihajlovski K. APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23. 2023;:179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23 (2023):179-186,
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Ilić, Nevena
AU  - Milošević, Jelena
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7030
AB  - By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
T1  - VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7030
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Ilić, Nevena and Milošević, Jelena and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "By-products of soybean oil production are high-quality protein supplements for
animal feed. However, they are rich in anti-nutritive factors and indigestible components,
among which the special attention is focused on galactooligosaccharides, due to lack of αgalactosidase in monogastric animals. Enzymatic treatment of soy-based products and
microbial fermentation are employed for overcoming these issues.
The main goal of this study was to apply fermentation with a selected strain of black
yeast-like fungus (Aureobasidium spp.) of the soybean products in order to obtain highprotein soy-based animal feed. First, screening for an appropriate strain of microorganism
among ten natural isolates from grapes has been performed. Keeping in mind complex
structure of soybean polysaccharides the first selection criterion was the ability of growth in
the presence of arabinose, xylose, galactose, mannose, raffinose, or soybean oil as sole
carbon source. More important, enzymatic activity of α-galactosidase was detected in new
isolates. The highest α-galactosidase producer was selected for cultivation on soybean meal.
The selected strain was characterized in terms of physiological and technological properties
and identified as Aureobasidium pullulans P8. In order to obtain high protein soybean meal, it
has been utilized in two types of fermentation, i.e. solid state (SSF) and submerged (SMF)
under varying conditions. Maximal protein content (61.11% based on dry weight) was
obtained after 5 days of SMF at 30 °C and 10% of dry matter, while SSF produced 57.78%
protein after 7 days of incubation at 30 °C with substrate contained 30% of dry matter.
Extracellular enzymatic activities of cellulase, pectinase, amylase, xylanase and αgalactosidase were detected in supernatant after SMF, indicating its potential reusability for
hydrolysis of new batch of soybean substrate.
This investigation revealed the versatile extracellular enzymatic potential of newly
isolated black yeast-like fungus Aureobasidium pullulans P8 and its potential for production
of high protein soybean meal.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023",
title = "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7030"
}

Davidović, S., Miljković, M., Ilić, N., Milošević, J.,& Dimitrijević-Branković, S.. (2023). VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 84.
https://hdl.handle.net/21.15107/rcub_technorep_7030

Davidović S, Miljković M, Ilić N, Milošević J, Dimitrijević-Branković S. VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023. 2023;:84.
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

Davidović, Slađana, Miljković, Miona, Ilić, Nevena, Milošević, Jelena, Dimitrijević-Branković, Suzana, "VALORIZATION OF SOYBEAN MEAL FOR PRODUCTION OF HIGH PROTEIN ANIMAL FEED AND VALUE-ADDED PRODUCTS USING NEW STRAIN OF AUREOBASIDIUM PULLULANS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023 (2023):84,
https://hdl.handle.net/21.15107/rcub_technorep_7030 .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Kostić, Anđela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5940
AB  - In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.
C3  - Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,
T1  - Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5940
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Kostić, Anđela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.",
journal = "Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,",
title = "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5940"
}

Ilić, N., Milić, M., Davidović, S., Kostić, A., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,, 80.
https://hdl.handle.net/21.15107/rcub_technorep_5940

Ilić N, Milić M, Davidović S, Kostić A, Mihajlovski K, Dimitrijević-Branković S. Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,. 2022;:80.
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Kostić, Anđela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix" in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering, (2022):80,
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

TY  - CONF
AU  - Vasić, Katja
AU  - Ilić, Nevena
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
AU  - Knez, Željko
AU  - Leitgeb, Maja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5941
AB  - The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.
PB  - Wiley
C3  - Book of Abstracts- Chemie Ingenieur Technik
T1  - Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde
IS  - 9
SP  - 1266
VL  - 94
DO  - 10.1002/cite.202255288
ER  - 

@conference{
author = "Vasić, Katja and Ilić, Nevena and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana and Knez, Željko and Leitgeb, Maja",
year = "2022",
abstract = "The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.",
publisher = "Wiley",
journal = "Book of Abstracts- Chemie Ingenieur Technik",
title = "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde",
number = "9",
pages = "1266",
volume = "94",
doi = "10.1002/cite.202255288"
}

Vasić, K., Ilić, N., Mihajlovski, K., Dimitrijević-Branković, S., Knez, Ž.,& Leitgeb, M.. (2022). Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik
Wiley., 94(9), 1266.
https://doi.org/10.1002/cite.202255288

Vasić K, Ilić N, Mihajlovski K, Dimitrijević-Branković S, Knez Ž, Leitgeb M. Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik. 2022;94(9):1266.
doi:10.1002/cite.202255288 .

Vasić, Katja, Ilić, Nevena, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, Knez, Željko, Leitgeb, Maja, "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde" in Book of Abstracts- Chemie Ingenieur Technik, 94, no. 9 (2022):1266,
https://doi.org/10.1002/cite.202255288 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Kukučka, Andrej
AU  - Milić, Marija
AU  - Milutinović, Milica
AU  - Miljković, Miona
AU  - Davidović, Slađana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5938
AB  - Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles
EP  - 469
SP  - 461
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5938
ER  - 

@conference{
author = "Ilić, Nevena and Kukučka, Andrej and Milić, Marija and Milutinović, Milica and Miljković, Miona and Davidović, Slađana",
year = "2021",
abstract = "Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles",
pages = "469-461",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5938"
}

Ilić, N., Kukučka, A., Milić, M., Milutinović, M., Miljković, M.,& Davidović, S.. (2021). Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938

Ilić N, Kukučka A, Milić M, Milutinović M, Miljković M, Davidović S. Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

Ilić, Nevena, Kukučka, Andrej, Milić, Marija, Milutinović, Milica, Miljković, Miona, Davidović, Slađana, "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):461-469,
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5939
AB  - The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.
C3  - Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021
T1  - The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5939
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.",
journal = "Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021",
title = "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5939"
}

Ilić, N., Milić, M., Davidović, S., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2021). The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021, 158.
https://hdl.handle.net/21.15107/rcub_technorep_5939

Ilić N, Milić M, Davidović S, Mihajlovski K, Dimitrijević-Branković S. The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021. 2021;:158.
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme" in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021 (2021):158,
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Lazić, Vesna
AU  - Radovanović, Neda
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Miljković, Miona
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5927
AB  - Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean
EP  - 460
SP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5927
ER  - 

@conference{
author = "Ilić, Nevena and Lazić, Vesna and Radovanović, Neda and Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona",
year = "2021",
abstract = "Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean",
pages = "460-451",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5927"
}

Ilić, N., Lazić, V., Radovanović, N., Mihajlovski, K., Davidović, S.,& Miljković, M.. (2021). Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927

Ilić N, Lazić V, Radovanović N, Mihajlovski K, Davidović S, Miljković M. Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

Ilić, Nevena, Lazić, Vesna, Radovanović, Neda, Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):451-460,
https://hdl.handle.net/21.15107/rcub_technorep_5927 .