@conference{
author = "Vasić, Katja and Primožić, Mateja and Leitgeb, Maja and Knez, Željko and Vukoičić, Ana and Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana",
year = "2023",
abstract = "In this work, immobilization of laccase from Trametes versicolor using different
immobilization techniques was investigated and obtained results regarding immobilized
enzyme activity were compared. The enzyme was successfully immobilized onto different
nanoparticles such as magnetic (MNPs) as well as cellulose (CNP) and silica nanoparticles
(SNP). Functionalized MNPs were prepared by co-precipitation of Fe2+ and Fe3+ ions,
which were initially coated with a layer of citric acid to prevent particle agglomeration.
Further, they were coated with sodium silicate and functionalized with aminosilane. MNPs
were activated with the crosslinking reagent glutaraldehyde and laccase was immobilized
onto functionalized MNPs. On the other hand, CNPs were prepared by ultrasonic treatment of
microcellulose while the SNPs were obtained from rice. Functionalization of these
nanoparticles was performed by introducing amino groups onto their surface, by modification
of CNPs with poly(ethyleneimine) and with organosilane in case of SNPs. Additionally, the
immobilized laccase in the form of cross-linked enzyme aggregates (Lac-CLEAs) was
prepared by simple immobilization method involving precipitation of the enzyme from
aqueous buffer using ethanol as precipitation solvent followed by cross-linking of aggregates
of enzyme molecules by glutaraldehyde. When the magnetic cross-linked enzyme aggregates
from laccase (Lac-mCLEAs) were synthesized, magnetic nanoparticles were added to the
synthesis process. The immobilization yield of laccase immobilized onto nanoparticles was
95%, 83% and 84% for MNPs, CNPs and SNPs, respectively, followed by activity yield of
77%, 73% and 66%, respectively. Some lower immobilization yield was achieved for the
Lac-CLEAs and Lac-mCLEAs, 74% and 75%, respectively. Lac-mCLEAs showed the
highest activity yield (85%) while activity yield of 76% was achieved for Lac-CLEAs when
BSA, as proteic feeder was used. Obtained results suggest that laccase could be successfully
immobilized using different supports and immobilization techniques.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6960"
}
