TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukić-Marković, Jelena
AU  - Petrović, Slobodan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2123
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
EP  - 14
SP  - 7
VL  - 72
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukić-Marković, Jelena and Petrović, Slobodan",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
pages = "14-7",
volume = "72",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukić-Marković, J.,& Petrović, S.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukić-Marković J, Petrović S. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukić-Marković, Jelena, Petrović, Slobodan, "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Tadić, Vanja
AU  - Bojović, Dragica
AU  - Arsić, Ivana
AU  - Đorđević, Sofija
AU  - Aksentijević, Ksenija
AU  - Stamenić, Marko
AU  - Janković, Slobodan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2207
AB  - Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
PB  - MDPI, Basel
T2  - Molecules
T1  - Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
EP  - 2703
IS  - 3
SP  - 2683
VL  - 17
DO  - 10.3390/molecules17032683
ER  - 

@article{
author = "Tadić, Vanja and Bojović, Dragica and Arsić, Ivana and Đorđević, Sofija and Aksentijević, Ksenija and Stamenić, Marko and Janković, Slobodan",
year = "2012",
abstract = "Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts",
pages = "2703-2683",
number = "3",
volume = "17",
doi = "10.3390/molecules17032683"
}

Tadić, V., Bojović, D., Arsić, I., Đorđević, S., Aksentijević, K., Stamenić, M.,& Janković, S.. (2012). Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules
MDPI, Basel., 17(3), 2683-2703.
https://doi.org/10.3390/molecules17032683

Tadić V, Bojović D, Arsić I, Đorđević S, Aksentijević K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules. 2012;17(3):2683-2703.
doi:10.3390/molecules17032683 .

Tadić, Vanja, Bojović, Dragica, Arsić, Ivana, Đorđević, Sofija, Aksentijević, Ksenija, Stamenić, Marko, Janković, Slobodan, "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts" in Molecules, 17, no. 3 (2012):2683-2703,
https://doi.org/10.3390/molecules17032683 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Stamenić, Marko
AU  - Ivanović, Jasna
AU  - Orlović, Aleksandar
AU  - Ristić, Mihailo S.
AU  - Đorđević, Sofija
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1136
AB  - Supercritical carbon dioxide extraction of sesquiterpenes from valerian root was investigated. Extractions from cultivar Arterner zuctung and two wild grown valerian subspecies were performed. The influence of extraction conditions on extraction yield and chemical composition of obtained extracts was investigated at temperatures from 40 degrees C to 50 degrees C and pressures from 10 MPa to 20 MPa. Chemical composition of obtained extracts was analyzed by GC/FID and GC/MS methods. The influence of particle size on extraction process was investigated at 40 degrees C and 10 MPa. The major constituents of supercritical extracts were valerianol, valerenal, bornyl acetate and kessanyl acetate. Optimal extraction conditions for the cyclopentanoid sesquiterepenes isolation, referred to the content of cyclopentanoid sesquiterpenes in extract, were found to be 50 degrees C and 15 MPa. Extract obtained from the cultivar was characterized by the higher content of cyclopentanoid sesquiterpenes, as well as valerenal comparing to extracts from wild grown species. At 40 degrees C and 20 MPa 19.6% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. At 40 degrees C and 10 MPa, 17.4% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. However, the extract obtained with methanol was characterized by the high content (42.51 %) of unwanted isovaleric as well as by the lower content of cyclopentanoid sequiterpenes than the extracts obtained with supercritical carbon dioxide. The process of supercritical extraction was influenced by particle size. In order to simulate the process, mathematical model on the secretory structure scale was derived. Results of the model showed good agreement with the experimental data.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Supercritical carbon dioxide extraction of sesquiterpenes from valerian root
EP  - 258
IS  - 2
SP  - 249
VL  - 43
DO  - 10.1016/j.supflu.2007.05.007
ER  - 

@article{
author = "Žižović, Irena and Stamenić, Marko and Ivanović, Jasna and Orlović, Aleksandar and Ristić, Mihailo S. and Đorđević, Sofija and Petrović, Slobodan and Skala, Dejan",
year = "2007",
abstract = "Supercritical carbon dioxide extraction of sesquiterpenes from valerian root was investigated. Extractions from cultivar Arterner zuctung and two wild grown valerian subspecies were performed. The influence of extraction conditions on extraction yield and chemical composition of obtained extracts was investigated at temperatures from 40 degrees C to 50 degrees C and pressures from 10 MPa to 20 MPa. Chemical composition of obtained extracts was analyzed by GC/FID and GC/MS methods. The influence of particle size on extraction process was investigated at 40 degrees C and 10 MPa. The major constituents of supercritical extracts were valerianol, valerenal, bornyl acetate and kessanyl acetate. Optimal extraction conditions for the cyclopentanoid sesquiterepenes isolation, referred to the content of cyclopentanoid sesquiterpenes in extract, were found to be 50 degrees C and 15 MPa. Extract obtained from the cultivar was characterized by the higher content of cyclopentanoid sesquiterpenes, as well as valerenal comparing to extracts from wild grown species. At 40 degrees C and 20 MPa 19.6% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. At 40 degrees C and 10 MPa, 17.4% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. However, the extract obtained with methanol was characterized by the high content (42.51 %) of unwanted isovaleric as well as by the lower content of cyclopentanoid sequiterpenes than the extracts obtained with supercritical carbon dioxide. The process of supercritical extraction was influenced by particle size. In order to simulate the process, mathematical model on the secretory structure scale was derived. Results of the model showed good agreement with the experimental data.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Supercritical carbon dioxide extraction of sesquiterpenes from valerian root",
pages = "258-249",
number = "2",
volume = "43",
doi = "10.1016/j.supflu.2007.05.007"
}

Žižović, I., Stamenić, M., Ivanović, J., Orlović, A., Ristić, M. S., Đorđević, S., Petrović, S.,& Skala, D.. (2007). Supercritical carbon dioxide extraction of sesquiterpenes from valerian root. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 43(2), 249-258.
https://doi.org/10.1016/j.supflu.2007.05.007

Žižović I, Stamenić M, Ivanović J, Orlović A, Ristić MS, Đorđević S, Petrović S, Skala D. Supercritical carbon dioxide extraction of sesquiterpenes from valerian root. in Journal of Supercritical Fluids. 2007;43(2):249-258.
doi:10.1016/j.supflu.2007.05.007 .

Žižović, Irena, Stamenić, Marko, Ivanović, Jasna, Orlović, Aleksandar, Ristić, Mihailo S., Đorđević, Sofija, Petrović, Slobodan, Skala, Dejan, "Supercritical carbon dioxide extraction of sesquiterpenes from valerian root" in Journal of Supercritical Fluids, 43, no. 2 (2007):249-258,
https://doi.org/10.1016/j.supflu.2007.05.007 . .