TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić VL, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić-Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3761
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine and Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić-Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine and Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić-Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić-Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić-Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine and Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .