TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4441
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić VL, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić VL, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4213
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
ER  - 

@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012"
}

Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012

Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012 .

Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4214
AB  - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP  - 390
SP  - 384
VL  - 213
DO  - 10.1016/j.saa.2019.01.069
ER  - 

@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069"
}

Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069

Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069 .

Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić-Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3761
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine and Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić-Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine and Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić-Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić-Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić-Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine and Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Đorđević, Verica
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Veljović, Đorđe
AU  - Misić, Danijela M.
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2738
AB  - The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
EP  - 259
SP  - 250
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Đorđević, Verica and Bukara, Katarina and Mojsilović, Slavko B. and Nedović, Viktor and Bugarski, Diana and Veljović, Đorđe and Misić, Danijela M. and Bugarski, Branko",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization",
pages = "259-250",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043"
}

Kostić, I. T., Ilić, V. Lj., Đorđević, V., Bukara, K., Mojsilović, S. B., Nedović, V., Bugarski, D., Veljović, Đ., Misić, D. M.,& Bugarski, B.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostić IT, Ilić VL, Đorđević V, Bukara K, Mojsilović SB, Nedović V, Bugarski D, Veljović Đ, Misić DM, Bugarski B. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostić, Ivana T., Ilić, Vesna Lj., Đorđević, Verica, Bukara, Katarina, Mojsilović, Slavko B., Nedović, Viktor, Bugarski, Diana, Veljović, Đorđe, Misić, Danijela M., Bugarski, Branko, "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization" in Colloids and Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .

TY  - JOUR
AU  - Stojanović, Radoslava
AU  - Ilić, Vesna Lj.
AU  - Manojlović, Verica
AU  - Bugarski, Diana
AU  - Dević, Marija
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2252
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.
PB  - Springer, New York
T2  - Applied Biochemistry and Biotechnology
T1  - Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor
EP  - 1506
IS  - 6
SP  - 1491
VL  - 166
DO  - 10.1007/s12010-012-9543-9
ER  - 

@article{
author = "Stojanović, Radoslava and Ilić, Vesna Lj. and Manojlović, Verica and Bugarski, Diana and Dević, Marija and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor",
pages = "1506-1491",
number = "6",
volume = "166",
doi = "10.1007/s12010-012-9543-9"
}

Stojanović, R., Ilić, V. Lj., Manojlović, V., Bugarski, D., Dević, M.,& Bugarski, B.. (2012). Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry and Biotechnology
Springer, New York., 166(6), 1491-1506.
https://doi.org/10.1007/s12010-012-9543-9

Stojanović R, Ilić VL, Manojlović V, Bugarski D, Dević M, Bugarski B. Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry and Biotechnology. 2012;166(6):1491-1506.
doi:10.1007/s12010-012-9543-9 .

Stojanović, Radoslava, Ilić, Vesna Lj., Manojlović, Verica, Bugarski, Diana, Dević, Marija, Bugarski, Branko, "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor" in Applied Biochemistry and Biotechnology, 166, no. 6 (2012):1491-1506,
https://doi.org/10.1007/s12010-012-9543-9 . .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Mojsilović, Slavko B.
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2103
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min.
AB  - Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes
T1  - Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom
EP  - 529
IS  - 4
SP  - 519
VL  - 66
DO  - 10.2298/HEMIND111122008S
ER  - 

@article{
author = "Pravilović, Radoslava and Mojsilović, Slavko B. and Kostić, Ivana T. and Ilić, Vesna Lj. and Bugarski, Diana and Đorđević, Verica and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min., Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes, Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom",
pages = "529-519",
number = "4",
volume = "66",
doi = "10.2298/HEMIND111122008S"
}

Pravilović, R., Mojsilović, S. B., Kostić, I. T., Ilić, V. Lj., Bugarski, D., Đorđević, V.,& Bugarski, B.. (2012). Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(4), 519-529.
https://doi.org/10.2298/HEMIND111122008S

Pravilović R, Mojsilović SB, Kostić IT, Ilić VL, Bugarski D, Đorđević V, Bugarski B. Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija. 2012;66(4):519-529.
doi:10.2298/HEMIND111122008S .

Pravilović, Radoslava, Mojsilović, Slavko B., Kostić, Ivana T., Ilić, Vesna Lj., Bugarski, Diana, Đorđević, Verica, Bugarski, Branko, "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes" in Hemijska industrija, 66, no. 4 (2012):519-529,
https://doi.org/10.2298/HEMIND111122008S . .

TY  - JOUR
AU  - Mihailović-Stanojević, Nevena
AU  - Bugarski, Branko
AU  - Komes, Drazenka
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Bugarski, Diana
AU  - Miloradović, Zoran
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1730
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Hypertension
T1  - The effect of wild thyme polyphenols on haemodynamic parameters and plasma lipides in spontaneously hypertensive rats
EP  - E495
SP  - E495
VL  - 28
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1730
ER  - 

@article{
author = "Mihailović-Stanojević, Nevena and Bugarski, Branko and Komes, Drazenka and Grujić-Milanović, Jelica and Ivanov, Milan and Jovović, Đurđica and Bugarski, Diana and Miloradović, Zoran",
year = "2010",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Hypertension",
title = "The effect of wild thyme polyphenols on haemodynamic parameters and plasma lipides in spontaneously hypertensive rats",
pages = "E495-E495",
volume = "28",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1730"
}

Mihailović-Stanojević, N., Bugarski, B., Komes, D., Grujić-Milanović, J., Ivanov, M., Jovović, Đ., Bugarski, D.,& Miloradović, Z.. (2010). The effect of wild thyme polyphenols on haemodynamic parameters and plasma lipides in spontaneously hypertensive rats. in Journal of Hypertension
Lippincott Williams & Wilkins, Philadelphia., 28, E495-E495.
https://hdl.handle.net/21.15107/rcub_technorep_1730

Mihailović-Stanojević N, Bugarski B, Komes D, Grujić-Milanović J, Ivanov M, Jovović Đ, Bugarski D, Miloradović Z. The effect of wild thyme polyphenols on haemodynamic parameters and plasma lipides in spontaneously hypertensive rats. in Journal of Hypertension. 2010;28:E495-E495.
https://hdl.handle.net/21.15107/rcub_technorep_1730 .

Mihailović-Stanojević, Nevena, Bugarski, Branko, Komes, Drazenka, Grujić-Milanović, Jelica, Ivanov, Milan, Jovović, Đurđica, Bugarski, Diana, Miloradović, Zoran, "The effect of wild thyme polyphenols on haemodynamic parameters and plasma lipides in spontaneously hypertensive rats" in Journal of Hypertension, 28 (2010):E495-E495,
https://hdl.handle.net/21.15107/rcub_technorep_1730 .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Osmokrović, Andrea
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1018
AB  - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
PB  - 8th Conference of the Yugoslav Materials Research Society
T2  - Materials Science Forum
T1  - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
EP  - 422
SP  - 417
VL  - 555
DO  - 10.4028/0-87849-441-3.417
ER  - 

@article{
author = "Obradović, Bojana and Osmokrović, Andrea and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2007",
abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.",
publisher = "8th Conference of the Yugoslav Materials Research Society",
journal = "Materials Science Forum",
title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies",
pages = "422-417",
volume = "555",
doi = "10.4028/0-87849-441-3.417"
}

Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum
8th Conference of the Yugoslav Materials Research Society., 555, 417-422.
https://doi.org/10.4028/0-87849-441-3.417

Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum. 2007;555:417-422.
doi:10.4028/0-87849-441-3.417 .

Obradović, Bojana, Osmokrović, Andrea, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Materials Science Forum, 555 (2007):417-422,
https://doi.org/10.4028/0-87849-441-3.417 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Diana
AU  - Plavšić, Milenko B.
AU  - Bugarski, Branko
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1088
AB  - A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.
PB  - Elsevier Sci Ltd, Oxford
T2  - Process Biochemistry
T1  - Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule
EP  - 174
IS  - 2
SP  - 167
VL  - 42
DO  - 10.1016/j.procbio.2006.07.023
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Bugarski, Diana and Plavšić, Milenko B. and Bugarski, Branko",
year = "2007",
abstract = "A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule",
pages = "174-167",
number = "2",
volume = "42",
doi = "10.1016/j.procbio.2006.07.023"
}

Pajić-Lijaković, I., Bugarski, D., Plavšić, M. B.,& Bugarski, B.. (2007). Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 42(2), 167-174.
https://doi.org/10.1016/j.procbio.2006.07.023

Pajić-Lijaković I, Bugarski D, Plavšić MB, Bugarski B. Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry. 2007;42(2):167-174.
doi:10.1016/j.procbio.2006.07.023 .

Pajić-Lijaković, Ivana, Bugarski, Diana, Plavšić, Milenko B., Bugarski, Branko, "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule" in Process Biochemistry, 42, no. 2 (2007):167-174,
https://doi.org/10.1016/j.procbio.2006.07.023 . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Obradović, Bojana
AU  - Bugarski, Diana
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 2006
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/897
AB  - Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering.
AB  - Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.
PB  - University of Belgrade - Faculty of Mechanical Engineering, Belgrade
T2  - FME Transactions
T1  - Development of a packed bed bioreactor for cartilage tissue engineering
T1  - Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice
EP  - 70
IS  - 2
SP  - 65
VL  - 34
UR  - https://hdl.handle.net/21.15107/rcub_technorep_897
ER  - 

@article{
author = "Osmokrović, Andrea and Obradović, Bojana and Bugarski, Diana and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "2006",
abstract = "Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering., Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.",
publisher = "University of Belgrade - Faculty of Mechanical Engineering, Belgrade",
journal = "FME Transactions",
title = "Development of a packed bed bioreactor for cartilage tissue engineering, Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice",
pages = "70-65",
number = "2",
volume = "34",
url = "https://hdl.handle.net/21.15107/rcub_technorep_897"
}

Osmokrović, A., Obradović, B., Bugarski, D., Bugarski, B.,& Vunjak-Novaković, G.. (2006). Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions
University of Belgrade - Faculty of Mechanical Engineering, Belgrade., 34(2), 65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897

Osmokrović A, Obradović B, Bugarski D, Bugarski B, Vunjak-Novaković G. Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions. 2006;34(2):65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897 .

Osmokrović, Andrea, Obradović, Bojana, Bugarski, Diana, Bugarski, Branko, Vunjak-Novaković, Gordana, "Development of a packed bed bioreactor for cartilage tissue engineering" in FME Transactions, 34, no. 2 (2006):65-70,
https://hdl.handle.net/21.15107/rcub_technorep_897 .

TY  - JOUR
AU  - Obradović, B.
AU  - Bugarski, Branko
AU  - Todosijević, Zoran
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/713
AB  - Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
PB  - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
T2  - Materials Science Forum
T1  - Mathematical modeling of cell distribution in alginate microbeads
EP  - 536
SP  - 531
VL  - 494
DO  - 10.4028/0-87849-971-7.531
ER  - 

@article{
author = "Obradović, B. and Bugarski, Branko and Todosijević, Zoran and Nedović, Viktor and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2005",
abstract = "Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Mathematical modeling of cell distribution in alginate microbeads",
pages = "536-531",
volume = "494",
doi = "10.4028/0-87849-971-7.531"
}

Obradović, B., Bugarski, B., Todosijević, Z., Nedović, V., Bugarski, D.,& Vunjak-Novaković, G.. (2005). Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 531-536.
https://doi.org/10.4028/0-87849-971-7.531

Obradović B, Bugarski B, Todosijević Z, Nedović V, Bugarski D, Vunjak-Novaković G. Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum. 2005;494:531-536.
doi:10.4028/0-87849-971-7.531 .

Obradović, B., Bugarski, Branko, Todosijević, Zoran, Nedović, Viktor, Bugarski, Diana, Vunjak-Novaković, Gordana, "Mathematical modeling of cell distribution in alginate microbeads" in Materials Science Forum, 494 (2005):531-536,
https://doi.org/10.4028/0-87849-971-7.531 . .

TY  - JOUR
AU  - Bugarski, Diana
AU  - Obradović, B.
AU  - Petakov, M
AU  - Jovcić, G
AU  - Stojanović, N
AU  - Bugarski, Branko
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/712
AB  - Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.
PB  - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
T2  - Materials Science Forum
T1  - Alginate microbeads as potential support for cultivation of bone marrow stromal cells
EP  - 530
SP  - 525
VL  - 494
DO  - 10.4028/0-87849-971-7.525
ER  - 

@article{
author = "Bugarski, Diana and Obradović, B. and Petakov, M and Jovcić, G and Stojanović, N and Bugarski, Branko",
year = "2005",
abstract = "Alginate is currently being employed and explored for a broad range of biomedical and biotechnology applications, due to its biodegradability and simple procedure for cell immobilization. However, cell immobilization was mostly aimed for immunoisolatory and biochemical processing applications and far less is known about potentials of alginate as a substrate for tissue formation. In the present work, isolation, immobilization and cultivation procedures of murine bone marrow stromal cells (BMSC) were studied and standardized in order to establish the alginate-bioreactor culture system for chondrogenic and/or hematopoiesis-supportive tissue progression. Two techniques for cell immobilization based on alginate were investigated: entrapment within gel matrix using electrostatic droplet generation and simple cell adsorption onto gel surfaces. Alginate gels in forms of microbeads and discs with immobilized culture expanded BMSC were cultivated for up to 30 days and analyzed for surface properties, cell concentration, viability, and differentiation.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Alginate microbeads as potential support for cultivation of bone marrow stromal cells",
pages = "530-525",
volume = "494",
doi = "10.4028/0-87849-971-7.525"
}

Bugarski, D., Obradović, B., Petakov, M., Jovcić, G., Stojanović, N.,& Bugarski, B.. (2005). Alginate microbeads as potential support for cultivation of bone marrow stromal cells. in Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 525-530.
https://doi.org/10.4028/0-87849-971-7.525

Bugarski D, Obradović B, Petakov M, Jovcić G, Stojanović N, Bugarski B. Alginate microbeads as potential support for cultivation of bone marrow stromal cells. in Materials Science Forum. 2005;494:525-530.
doi:10.4028/0-87849-971-7.525 .

Bugarski, Diana, Obradović, B., Petakov, M, Jovcić, G, Stojanović, N, Bugarski, Branko, "Alginate microbeads as potential support for cultivation of bone marrow stromal cells" in Materials Science Forum, 494 (2005):525-530,
https://doi.org/10.4028/0-87849-971-7.525 . .

TY  - JOUR
AU  - Obradović, B.
AU  - Bugarski, Diana
AU  - Petakov, M
AU  - Jovcić, G
AU  - Stojanović, N
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 2004
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/673
AB  - Cartilage tissue engineering based on chondrogenic cells seeded onto biodegradable polymer supports and cultivated in bioreactors can potentially become an effective method for creating functional tissue equivalents. Cultivation in perfused bioreactors can improve the uniformity and structure of the engineered tissues. We report studies of two different supports for immunobilization of mouse bone marrow stromal cells (BMSC) for cultivation in perfused bioreactors: fibrous polyglycolic acid (PGA) scaffolds and alginate microbeads.
PB  - Trans Tech Publications Ltd, Durnten-Zurich
T2  - Progress in Advanced Materials and Processes
T1  - Cell support studies aimed for cartilage tissue engineering in perfused bioreactors
EP  - 553
SP  - 549
VL  - 453-454
DO  - 10.4028/www.scientific.net/MSF.453-454.549
ER  - 

@article{
author = "Obradović, B. and Bugarski, Diana and Petakov, M and Jovcić, G and Stojanović, N and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "2004",
abstract = "Cartilage tissue engineering based on chondrogenic cells seeded onto biodegradable polymer supports and cultivated in bioreactors can potentially become an effective method for creating functional tissue equivalents. Cultivation in perfused bioreactors can improve the uniformity and structure of the engineered tissues. We report studies of two different supports for immunobilization of mouse bone marrow stromal cells (BMSC) for cultivation in perfused bioreactors: fibrous polyglycolic acid (PGA) scaffolds and alginate microbeads.",
publisher = "Trans Tech Publications Ltd, Durnten-Zurich",
journal = "Progress in Advanced Materials and Processes",
title = "Cell support studies aimed for cartilage tissue engineering in perfused bioreactors",
pages = "553-549",
volume = "453-454",
doi = "10.4028/www.scientific.net/MSF.453-454.549"
}

Obradović, B., Bugarski, D., Petakov, M., Jovcić, G., Stojanović, N., Bugarski, B.,& Vunjak-Novaković, G.. (2004). Cell support studies aimed for cartilage tissue engineering in perfused bioreactors. in Progress in Advanced Materials and Processes
Trans Tech Publications Ltd, Durnten-Zurich., 453-454, 549-553.
https://doi.org/10.4028/www.scientific.net/MSF.453-454.549

Obradović B, Bugarski D, Petakov M, Jovcić G, Stojanović N, Bugarski B, Vunjak-Novaković G. Cell support studies aimed for cartilage tissue engineering in perfused bioreactors. in Progress in Advanced Materials and Processes. 2004;453-454:549-553.
doi:10.4028/www.scientific.net/MSF.453-454.549 .

Obradović, B., Bugarski, Diana, Petakov, M, Jovcić, G, Stojanović, N, Bugarski, Branko, Vunjak-Novaković, Gordana, "Cell support studies aimed for cartilage tissue engineering in perfused bioreactors" in Progress in Advanced Materials and Processes, 453-454 (2004):549-553,
https://doi.org/10.4028/www.scientific.net/MSF.453-454.549 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2004
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/637
AB  - In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue.
AB  - U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.
PB  - Institut bezbednosti, Beograd
T2  - Nauka, tehnika, bezbednost
T1  - In vitro tissue cultures: Microencapsulation and bioreactor cultivation
T1  - In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija
EP  - 68
IS  - 2
SP  - 59
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_technorep_637
ER  - 

@article{
author = "Obradović, Bojana and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2004",
abstract = "In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue., U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.",
publisher = "Institut bezbednosti, Beograd",
journal = "Nauka, tehnika, bezbednost",
title = "In vitro tissue cultures: Microencapsulation and bioreactor cultivation, In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija",
pages = "68-59",
number = "2",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_technorep_637"
}

Obradović, B., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2004). In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost
Institut bezbednosti, Beograd., 14(2), 59-68.
https://hdl.handle.net/21.15107/rcub_technorep_637

Obradović B, Bugarski B, Bugarski D, Vunjak-Novaković G. In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost. 2004;14(2):59-68.
https://hdl.handle.net/21.15107/rcub_technorep_637 .

Obradović, Bojana, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "In vitro tissue cultures: Microencapsulation and bioreactor cultivation" in Nauka, tehnika, bezbednost, 14, no. 2 (2004):59-68,
https://hdl.handle.net/21.15107/rcub_technorep_637 .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Branko
AU  - Obradović, Bojana
AU  - Plavšić, Milenko B.
AU  - Bugarski, Diana
PY  - 2003
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/560
AB  - Development of lipid-based fine particles as potential drug carriers requires detailed investigation of possible effects of these carriers on rheological properties of blood. In this study, we have investigated the influence of dynamic conditions on aggregate formation and stability in dispersions of lipid-based fine particles in whole blood under in vitro conditions. Rheological parameters of two concentrations of liposome dispersion and two concentrations of lipid emulsion in blood were studied by assessing shear stress/shear rate relationships. The magnitude of attractive interactions between aggregates and/or particles, A, and the effective-to-real volume fraction of particles, phi(f)/phi(p) , were estimated for rheological quantification of lipid-based fine particles-blood interactions and aggregate stability. Addition of lipid-based particles induced aggregate formation in blood, which was more pronounced at higher concentrations of lipid-based fine particles. Furthermore, larger and more stable aggregates were formed in liposome dispersions as compared to lipid emulsions in blood.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Chemical Engineering Communications
T1  - Examination of rheological properties of fine particles as carriers for controlled drug release
EP  - 93
IS  - 1
SP  - 83
VL  - 190
DO  - 10.1080/00986440302091
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Bugarski, Branko and Obradović, Bojana and Plavšić, Milenko B. and Bugarski, Diana",
year = "2003",
abstract = "Development of lipid-based fine particles as potential drug carriers requires detailed investigation of possible effects of these carriers on rheological properties of blood. In this study, we have investigated the influence of dynamic conditions on aggregate formation and stability in dispersions of lipid-based fine particles in whole blood under in vitro conditions. Rheological parameters of two concentrations of liposome dispersion and two concentrations of lipid emulsion in blood were studied by assessing shear stress/shear rate relationships. The magnitude of attractive interactions between aggregates and/or particles, A, and the effective-to-real volume fraction of particles, phi(f)/phi(p) , were estimated for rheological quantification of lipid-based fine particles-blood interactions and aggregate stability. Addition of lipid-based particles induced aggregate formation in blood, which was more pronounced at higher concentrations of lipid-based fine particles. Furthermore, larger and more stable aggregates were formed in liposome dispersions as compared to lipid emulsions in blood.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Chemical Engineering Communications",
title = "Examination of rheological properties of fine particles as carriers for controlled drug release",
pages = "93-83",
number = "1",
volume = "190",
doi = "10.1080/00986440302091"
}

Pajić-Lijaković, I., Bugarski, B., Obradović, B., Plavšić, M. B.,& Bugarski, D.. (2003). Examination of rheological properties of fine particles as carriers for controlled drug release. in Chemical Engineering Communications
Taylor & Francis Inc, Philadelphia., 190(1), 83-93.
https://doi.org/10.1080/00986440302091

Pajić-Lijaković I, Bugarski B, Obradović B, Plavšić MB, Bugarski D. Examination of rheological properties of fine particles as carriers for controlled drug release. in Chemical Engineering Communications. 2003;190(1):83-93.
doi:10.1080/00986440302091 .

Pajić-Lijaković, Ivana, Bugarski, Branko, Obradović, Bojana, Plavšić, Milenko B., Bugarski, Diana, "Examination of rheological properties of fine particles as carriers for controlled drug release" in Chemical Engineering Communications, 190, no. 1 (2003):83-93,
https://doi.org/10.1080/00986440302091 . .

TY  - JOUR
AU  - Stojanović, N
AU  - Jovcić, G
AU  - Bugarski, Diana
AU  - Petakov, M
AU  - Kataranovski, M
AU  - Pavkov, R
AU  - Knežević, D
AU  - Mojović, Ljiljana
AU  - Bugarski, Branko
PY  - 1996
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/90
PB  - Elsevier Science Inc, New York
T2  - Experimental Hematology
T1  - Liposomes - Are they adequate delivery system for hemoglobin?
EP  - 232
IS  - 9
SP  - 232
VL  - 24
UR  - https://hdl.handle.net/21.15107/rcub_technorep_90
ER  - 

@article{
author = "Stojanović, N and Jovcić, G and Bugarski, Diana and Petakov, M and Kataranovski, M and Pavkov, R and Knežević, D and Mojović, Ljiljana and Bugarski, Branko",
year = "1996",
publisher = "Elsevier Science Inc, New York",
journal = "Experimental Hematology",
title = "Liposomes - Are they adequate delivery system for hemoglobin?",
pages = "232-232",
number = "9",
volume = "24",
url = "https://hdl.handle.net/21.15107/rcub_technorep_90"
}

Stojanović, N., Jovcić, G., Bugarski, D., Petakov, M., Kataranovski, M., Pavkov, R., Knežević, D., Mojović, L.,& Bugarski, B.. (1996). Liposomes - Are they adequate delivery system for hemoglobin?. in Experimental Hematology
Elsevier Science Inc, New York., 24(9), 232-232.
https://hdl.handle.net/21.15107/rcub_technorep_90

Stojanović N, Jovcić G, Bugarski D, Petakov M, Kataranovski M, Pavkov R, Knežević D, Mojović L, Bugarski B. Liposomes - Are they adequate delivery system for hemoglobin?. in Experimental Hematology. 1996;24(9):232-232.
https://hdl.handle.net/21.15107/rcub_technorep_90 .

Stojanović, N, Jovcić, G, Bugarski, Diana, Petakov, M, Kataranovski, M, Pavkov, R, Knežević, D, Mojović, Ljiljana, Bugarski, Branko, "Liposomes - Are they adequate delivery system for hemoglobin?" in Experimental Hematology, 24, no. 9 (1996):232-232,
https://hdl.handle.net/21.15107/rcub_technorep_90 .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Šiler-Marinković, Slavica
AU  - Bugarski, Diana
AU  - Jovcić, G
AU  - Petakov, M
AU  - Bugarski, Branko
PY  - 1996
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/84
AB  - Liposomes, closed spherical structures formed by phospholipid bilayers, have been recognized as a controlled drug delivery system. We have studied the preparation and biocompatibility of liposomes based on soya phospholipids encapsulated with alpha-tocopherol. The binding efficiency of alpha-tocopherol was analysed by comparing two methods for liposome preparation: a) dry film (DFM) and b) solvent infusion method (SIM). The degree of encapsulation achieved (88-93%) suggested a high affinity of alpha-tocopherol for liposome membrane binding. The initial concentration of alpha-tocopherol had a significant effect on the degree of encapsulation, while the effect of method used was less pronounced. In general, a higher degree of encapsulation was achieved with smaller liposome size fractions. Based on an experimentally obtained size distribution function, it can be concluded that if smaller liposomes are used, SIM seems to be more efficient due to the higher content of smaller vesicles. The in vivo application of the liposomes to CBA mice confirmed the biocompatibility and nontoxicity of such preparations. The analysis of hematological parameters in peripheral blood (determination of mature blood cells with differential count) revealed that liposomes did not express cytotoxic effects on any of the parameters tested.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria, Beograd
T1  - Liposomes with alpha-tocopherol membrane
EP  - 202
IS  - 4
SP  - 193
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_technorep_84
ER  - 

@article{
author = "Mojović, Ljiljana and Šiler-Marinković, Slavica and Bugarski, Diana and Jovcić, G and Petakov, M and Bugarski, Branko",
year = "1996",
abstract = "Liposomes, closed spherical structures formed by phospholipid bilayers, have been recognized as a controlled drug delivery system. We have studied the preparation and biocompatibility of liposomes based on soya phospholipids encapsulated with alpha-tocopherol. The binding efficiency of alpha-tocopherol was analysed by comparing two methods for liposome preparation: a) dry film (DFM) and b) solvent infusion method (SIM). The degree of encapsulation achieved (88-93%) suggested a high affinity of alpha-tocopherol for liposome membrane binding. The initial concentration of alpha-tocopherol had a significant effect on the degree of encapsulation, while the effect of method used was less pronounced. In general, a higher degree of encapsulation was achieved with smaller liposome size fractions. Based on an experimentally obtained size distribution function, it can be concluded that if smaller liposomes are used, SIM seems to be more efficient due to the higher content of smaller vesicles. The in vivo application of the liposomes to CBA mice confirmed the biocompatibility and nontoxicity of such preparations. The analysis of hematological parameters in peripheral blood (determination of mature blood cells with differential count) revealed that liposomes did not express cytotoxic effects on any of the parameters tested.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria, Beograd",
title = "Liposomes with alpha-tocopherol membrane",
pages = "202-193",
number = "4",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_technorep_84"
}

Mojović, L., Šiler-Marinković, S., Bugarski, D., Jovcić, G., Petakov, M.,& Bugarski, B.. (1996). Liposomes with alpha-tocopherol membrane. in Acta Veterinaria, Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 46(4), 193-202.
https://hdl.handle.net/21.15107/rcub_technorep_84

Mojović L, Šiler-Marinković S, Bugarski D, Jovcić G, Petakov M, Bugarski B. Liposomes with alpha-tocopherol membrane. in Acta Veterinaria, Beograd. 1996;46(4):193-202.
https://hdl.handle.net/21.15107/rcub_technorep_84 .

Mojović, Ljiljana, Šiler-Marinković, Slavica, Bugarski, Diana, Jovcić, G, Petakov, M, Bugarski, Branko, "Liposomes with alpha-tocopherol membrane" in Acta Veterinaria, Beograd, 46, no. 4 (1996):193-202,
https://hdl.handle.net/21.15107/rcub_technorep_84 .