TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Miljković, Miona
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Filipović, Jovanka
AU  - Tomić, Simonida
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3709
AB  - The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.
PB  - Inst Chemical Engineers, Rugby
T2  - Chemical Engineering Research & Design
T1  - Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels
EP  - 380
SP  - 368
VL  - 121
DO  - 10.1016/j.cherd.2017.03.030
ER  - 

@article{
author = "Krezović, Bojana D. and Miljković, Miona and Stojanović, Sanja T. and Najman, Stevo and Filipović, Jovanka and Tomić, Simonida",
year = "2017",
abstract = "The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Chemical Engineering Research & Design",
title = "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels",
pages = "380-368",
volume = "121",
doi = "10.1016/j.cherd.2017.03.030"
}

Krezović, B. D., Miljković, M., Stojanović, S. T., Najman, S., Filipović, J.,& Tomić, S.. (2017). Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design
Inst Chemical Engineers, Rugby., 121, 368-380.
https://doi.org/10.1016/j.cherd.2017.03.030

Krezović BD, Miljković M, Stojanović ST, Najman S, Filipović J, Tomić S. Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design. 2017;121:368-380.
doi:10.1016/j.cherd.2017.03.030 .

Krezović, Bojana D., Miljković, Miona, Stojanović, Sanja T., Najman, Stevo, Filipović, Jovanka, Tomić, Simonida, "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels" in Chemical Engineering Research & Design, 121 (2017):368-380,
https://doi.org/10.1016/j.cherd.2017.03.030 . .

TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Dimitrijević, Suzana
AU  - Filipović, Jovanka M.
AU  - Nikolić, Rajko R.
AU  - Tomić, Simonida
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2543
AB  - New semi-interpenetrating networks (semi-IPNHs) (P(HEMA/IA)/PVP) were prepared by free radical crosslinking copolymerization of 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA, 5 mol%), in the presence of poly(vinyl pyrrolidone) (PVP, 2, 5, and 10 mol%), as an interpenetrating agent. The structure of the semi-IPNHs was confirmed by Fourier transform infrared spectroscopy, and morphology study was performed by scanning electron microscopy, which revealed the characteristic porous morphology. The results obtained by dynamic mechanical analysis showed the improvement of mechanical properties with increasing PVP content in semi-IPNs. The maximum swelling was observed for all studied systems at a slightly acidic media (around pH 6), so it can be said that the content of PVP has no influence on the swelling behavior, in the PVP range investigated. Along with the pH sensitivity, which was expected due to the presence of IA, semi-IPNHs showed temperature-sensitive swelling properties, with the lower critical solution temperature value around 41 degrees C, which is in the physiologically interesting interval. The antimicrobial activity of the samples was tested using E. coli, S. aureus, and C. albicans pathogens. It was noticed that the antimicrobial potential depends on type of microbes, time of exposure, and PVP content in the samples. Due to their good antimicrobial and mechanical properties these stimuli-sensitive semi-IPNHs have potential to be used as biomaterials for the applications in medicine and pharmacy.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels
EP  - 819
IS  - 3
SP  - 809
VL  - 70
DO  - 10.1007/s00289-012-0830-y
ER  - 

@article{
author = "Krezović, Bojana D. and Dimitrijević, Suzana and Filipović, Jovanka M. and Nikolić, Rajko R. and Tomić, Simonida",
year = "2013",
abstract = "New semi-interpenetrating networks (semi-IPNHs) (P(HEMA/IA)/PVP) were prepared by free radical crosslinking copolymerization of 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA, 5 mol%), in the presence of poly(vinyl pyrrolidone) (PVP, 2, 5, and 10 mol%), as an interpenetrating agent. The structure of the semi-IPNHs was confirmed by Fourier transform infrared spectroscopy, and morphology study was performed by scanning electron microscopy, which revealed the characteristic porous morphology. The results obtained by dynamic mechanical analysis showed the improvement of mechanical properties with increasing PVP content in semi-IPNs. The maximum swelling was observed for all studied systems at a slightly acidic media (around pH 6), so it can be said that the content of PVP has no influence on the swelling behavior, in the PVP range investigated. Along with the pH sensitivity, which was expected due to the presence of IA, semi-IPNHs showed temperature-sensitive swelling properties, with the lower critical solution temperature value around 41 degrees C, which is in the physiologically interesting interval. The antimicrobial activity of the samples was tested using E. coli, S. aureus, and C. albicans pathogens. It was noticed that the antimicrobial potential depends on type of microbes, time of exposure, and PVP content in the samples. Due to their good antimicrobial and mechanical properties these stimuli-sensitive semi-IPNHs have potential to be used as biomaterials for the applications in medicine and pharmacy.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels",
pages = "819-809",
number = "3",
volume = "70",
doi = "10.1007/s00289-012-0830-y"
}

Krezović, B. D., Dimitrijević, S., Filipović, J. M., Nikolić, R. R.,& Tomić, S.. (2013). Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels. in Polymer Bulletin
Springer, New York., 70(3), 809-819.
https://doi.org/10.1007/s00289-012-0830-y

Krezović BD, Dimitrijević S, Filipović JM, Nikolić RR, Tomić S. Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels. in Polymer Bulletin. 2013;70(3):809-819.
doi:10.1007/s00289-012-0830-y .

Krezović, Bojana D., Dimitrijević, Suzana, Filipović, Jovanka M., Nikolić, Rajko R., Tomić, Simonida, "Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels" in Polymer Bulletin, 70, no. 3 (2013):809-819,
https://doi.org/10.1007/s00289-012-0830-y . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Mićić, Maja M.
AU  - Krezović, Bojana D.
AU  - Dobić, Sava N.
AU  - Suljovrujić, Edin H.
AU  - Filipović, Jovanka M.
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1400
AB  - pH and temperature sensitive hydrogels, based on 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA) copolymers, were prepared by gamma irradiation and characterized in order to examine their potential use in biomedical applications. The influence of comonomer ratio in these smart copolymers on their morphology, mechanical properties, biocompatibility and microbe penetration capability was investigated. The mechanical properties of copolymers were investigated using the dynamic mechanical analysis (DMA), while their morphology was examined by scanning electron microscopy (SEM). The morphology and mechanical properties of these hydrogels were found to be suitable for most requirements of biomedical applications. The in vitro study of P(HEMA/IA) biocompatibility showed no evidence of cell toxicity nor any considerable hemolytic activity. Furthermore, the microbe penetration test showed that neither Staphylococcus aureus nor Escherichia coli passed through the hydogel dressing; thus the P(HEMA/IA) dressing could be considered a good barrier against microbes. All results indicate that stimuli-responsive P(HEMA/IA) hydrogels have great potential for biomedical applications, especially for skin treatment and wound dressings.
AB  - pH i temperaturno osetljivi hidrogelovi na bazi kopolimera 2-hidroksietilmetakrilata (HEMA) i itakonske kiseline (IK) sintetisani su upotrebom gama zračenja i karakterisani s ciljem ispitivanja njihove potencijalne primene kao biomaterijala. Ispitivan je uticaj odnosa komonomera u ovim inteligentnim polimernim biomaterijalima na morfologiju, mehanička svojstva, biokompatibilnost i penetraciju mikroba. Mehanička svojstva kopolimera su ispitivana dinamičko-mehaničkom analizom (DMA), dok je morfologija karakterisana skenirajućom elektronskom mikroskopijom (SEM). Pokazalo se da su morfologija i mehanička svojstva ovih hidrogelova odgovarajući za većinu biomedicinskih primena. In vitro analizom biokompatibilnosti P(HEMA/IK) hidrogelova potvrđeno je da nisu toksični za ćelije niti izazivaju značajnu hemolitičku aktivnost. Takođe, test mikrobne penetracije pokazao je da ni Staphylococcus aureus ni Escherichia coli ne prolaze kroz hidrogelne obloge; stoga se P(HEMA/IK) obloge mogu smatrati dobrom barijerom protiv mikroba, a radi održavanja sterilnosti okolne sredine. Svi rezultati pokazuju da P(HEMA/IK) hidrogelovi imaju veliku mogućnost primene u biomedicini, posebno kod tretmana kože i kao obloge za opekotine.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Smart hydrogels based on itaconic acid for biomedical application
T1  - Inteligentni hidrogelovi na bazi itakonske kiseline za biomedicinsku primenu
EP  - 610
IS  - 6
SP  - 603
VL  - 63
DO  - 10.2298/HEMIND0906603T
ER  - 

@article{
author = "Tomić, Simonida and Mićić, Maja M. and Krezović, Bojana D. and Dobić, Sava N. and Suljovrujić, Edin H. and Filipović, Jovanka M.",
year = "2009",
abstract = "pH and temperature sensitive hydrogels, based on 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA) copolymers, were prepared by gamma irradiation and characterized in order to examine their potential use in biomedical applications. The influence of comonomer ratio in these smart copolymers on their morphology, mechanical properties, biocompatibility and microbe penetration capability was investigated. The mechanical properties of copolymers were investigated using the dynamic mechanical analysis (DMA), while their morphology was examined by scanning electron microscopy (SEM). The morphology and mechanical properties of these hydrogels were found to be suitable for most requirements of biomedical applications. The in vitro study of P(HEMA/IA) biocompatibility showed no evidence of cell toxicity nor any considerable hemolytic activity. Furthermore, the microbe penetration test showed that neither Staphylococcus aureus nor Escherichia coli passed through the hydogel dressing; thus the P(HEMA/IA) dressing could be considered a good barrier against microbes. All results indicate that stimuli-responsive P(HEMA/IA) hydrogels have great potential for biomedical applications, especially for skin treatment and wound dressings., pH i temperaturno osetljivi hidrogelovi na bazi kopolimera 2-hidroksietilmetakrilata (HEMA) i itakonske kiseline (IK) sintetisani su upotrebom gama zračenja i karakterisani s ciljem ispitivanja njihove potencijalne primene kao biomaterijala. Ispitivan je uticaj odnosa komonomera u ovim inteligentnim polimernim biomaterijalima na morfologiju, mehanička svojstva, biokompatibilnost i penetraciju mikroba. Mehanička svojstva kopolimera su ispitivana dinamičko-mehaničkom analizom (DMA), dok je morfologija karakterisana skenirajućom elektronskom mikroskopijom (SEM). Pokazalo se da su morfologija i mehanička svojstva ovih hidrogelova odgovarajući za većinu biomedicinskih primena. In vitro analizom biokompatibilnosti P(HEMA/IK) hidrogelova potvrđeno je da nisu toksični za ćelije niti izazivaju značajnu hemolitičku aktivnost. Takođe, test mikrobne penetracije pokazao je da ni Staphylococcus aureus ni Escherichia coli ne prolaze kroz hidrogelne obloge; stoga se P(HEMA/IK) obloge mogu smatrati dobrom barijerom protiv mikroba, a radi održavanja sterilnosti okolne sredine. Svi rezultati pokazuju da P(HEMA/IK) hidrogelovi imaju veliku mogućnost primene u biomedicini, posebno kod tretmana kože i kao obloge za opekotine.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Smart hydrogels based on itaconic acid for biomedical application, Inteligentni hidrogelovi na bazi itakonske kiseline za biomedicinsku primenu",
pages = "610-603",
number = "6",
volume = "63",
doi = "10.2298/HEMIND0906603T"
}

Tomić, S., Mićić, M. M., Krezović, B. D., Dobić, S. N., Suljovrujić, E. H.,& Filipović, J. M.. (2009). Smart hydrogels based on itaconic acid for biomedical application. in Hemijska industrija
Association of Chemical Engineers of Serbia., 63(6), 603-610.
https://doi.org/10.2298/HEMIND0906603T

Tomić S, Mićić MM, Krezović BD, Dobić SN, Suljovrujić EH, Filipović JM. Smart hydrogels based on itaconic acid for biomedical application. in Hemijska industrija. 2009;63(6):603-610.
doi:10.2298/HEMIND0906603T .

Tomić, Simonida, Mićić, Maja M., Krezović, Bojana D., Dobić, Sava N., Suljovrujić, Edin H., Filipović, Jovanka M., "Smart hydrogels based on itaconic acid for biomedical application" in Hemijska industrija, 63, no. 6 (2009):603-610,
https://doi.org/10.2298/HEMIND0906603T . .