TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3726
AB  - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
EP  - 348
SP  - 341
VL  - 155
DO  - 10.1016/j.colsurfb.2017.04.044
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Miljković, Miona and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2017",
abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study",
pages = "348-341",
volume = "155",
doi = "10.1016/j.colsurfb.2017.04.044"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S., Miljković, M., Kascakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 155, 341-348.
https://doi.org/10.1016/j.colsurfb.2017.04.044

Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković S, Miljković M, Kascakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces B-Biointerfaces. 2017;155:341-348.
doi:10.1016/j.colsurfb.2017.04.044 .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana, Miljković, Miona, Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces B-Biointerfaces, 155 (2017):341-348,
https://doi.org/10.1016/j.colsurfb.2017.04.044 . .

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Markelić, Milica
AU  - Đoković, Vladimir
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3256
AB  - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
PB  - Springer, Dordrecht
T2  - Optical and Quantum Electronics
T1  - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
IS  - 6
VL  - 48
DO  - 10.1007/s11082-016-0578-y
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Kascakova, Slavka and Refregiers, Matthieu and Markelić, Milica and Đoković, Vladimir",
year = "2016",
abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.",
publisher = "Springer, Dordrecht",
journal = "Optical and Quantum Electronics",
title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids",
number = "6",
volume = "48",
doi = "10.1007/s11082-016-0578-y"
}

Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S., Kascakova, S., Refregiers, M., Markelić, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics
Springer, Dordrecht., 48(6).
https://doi.org/10.1007/s11082-016-0578-y

Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković S, Kascakova S, Refregiers M, Markelić M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6).
doi:10.1007/s11082-016-0578-y .

Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana, Kascakova, Slavka, Refregiers, Matthieu, Markelić, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016),
https://doi.org/10.1007/s11082-016-0578-y . .

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana
AU  - Milosavljević, Aleksandar R.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3454
AB  - The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.
PB  - Royal Soc Chemistry, Cambridge
T2  - Analyst
T1  - A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution
EP  - 1996
IS  - 6
SP  - 1988
VL  - 141
DO  - 10.1039/c5an02358k
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Milosavljević, Aleksandar R. and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2016",
abstract = "The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Analyst",
title = "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution",
pages = "1996-1988",
number = "6",
volume = "141",
doi = "10.1039/c5an02358k"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S., Milosavljević, A. R., Kascakova, S., Refregiers, M.,& Đoković, V.. (2016). A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst
Royal Soc Chemistry, Cambridge., 141(6), 1988-1996.
https://doi.org/10.1039/c5an02358k

Dojčilović R, Pajović JD, Božanić DK, Vodnik V, Dimitrijević-Branković S, Milosavljević AR, Kascakova S, Refregiers M, Đoković V. A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst. 2016;141(6):1988-1996.
doi:10.1039/c5an02358k .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana, Milosavljević, Aleksandar R., Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution" in Analyst, 141, no. 6 (2016):1988-1996,
https://doi.org/10.1039/c5an02358k . .

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Dimitrijević-Branković, Suzana
AU  - Vodnik, Vesna
AU  - Milosavljević, Aleksandar R.
AU  - Piscopiello, Emanuela
AU  - Luyt, Adriaan S.
AU  - Đoković, Vladimir
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3102
AB  - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
EP  - 750
SP  - 742
VL  - 135
DO  - 10.1016/j.colsurfb.2015.08.050
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
pages = "750-742",
volume = "135",
doi = "10.1016/j.colsurfb.2015.08.050"
}

Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050

Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković S, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces. 2015;135:742-750.
doi:10.1016/j.colsurfb.2015.08.050 .

Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kascakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana, Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces B-Biointerfaces, 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 . .