TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana
AU  - Milosavljević, Aleksandar R.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3454
AB  - The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.
PB  - Royal Soc Chemistry, Cambridge
T2  - Analyst
T1  - A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution
EP  - 1996
IS  - 6
SP  - 1988
VL  - 141
DO  - 10.1039/c5an02358k
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana and Milosavljević, Aleksandar R. and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2016",
abstract = "The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Analyst",
title = "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution",
pages = "1996-1988",
number = "6",
volume = "141",
doi = "10.1039/c5an02358k"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S., Milosavljević, A. R., Kascakova, S., Refregiers, M.,& Đoković, V.. (2016). A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst
Royal Soc Chemistry, Cambridge., 141(6), 1988-1996.
https://doi.org/10.1039/c5an02358k

Dojčilović R, Pajović JD, Božanić DK, Vodnik V, Dimitrijević-Branković S, Milosavljević AR, Kascakova S, Refregiers M, Đoković V. A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst. 2016;141(6):1988-1996.
doi:10.1039/c5an02358k .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana, Milosavljević, Aleksandar R., Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution" in Analyst, 141, no. 6 (2016):1988-1996,
https://doi.org/10.1039/c5an02358k . .

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Dimitrijević-Branković, Suzana
AU  - Vodnik, Vesna
AU  - Milosavljević, Aleksandar R.
AU  - Piscopiello, Emanuela
AU  - Luyt, Adriaan S.
AU  - Đoković, Vladimir
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3102
AB  - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
EP  - 750
SP  - 742
VL  - 135
DO  - 10.1016/j.colsurfb.2015.08.050
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
pages = "750-742",
volume = "135",
doi = "10.1016/j.colsurfb.2015.08.050"
}

Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050

Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković S, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces B-Biointerfaces. 2015;135:742-750.
doi:10.1016/j.colsurfb.2015.08.050 .

Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kascakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana, Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces B-Biointerfaces, 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 . .