TY  - JOUR
AU  - Durdević, Sanja
AU  - Šavikin, Katarina
AU  - Živković, Jelena Č.
AU  - Boehm, Volker
AU  - Stanojković, Tatjana
AU  - Damjanović, Ana
AU  - Petrović, Slobodan
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961
AB  - Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.
EP  - 232
SP  - 225
VL  - 133
DO  - 10.1016/j.supflu.2017.10.021
ER  - 

@article{
author = "Durdević, Sanja and Šavikin, Katarina and Živković, Jelena Č. and Boehm, Volker and Stanojković, Tatjana and Damjanović, Ana and Petrović, Slobodan",
year = "2018",
abstract = "Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.",
pages = "232-225",
volume = "133",
doi = "10.1016/j.supflu.2017.10.021"
}

Durdević, S., Šavikin, K., Živković, J. Č., Boehm, V., Stanojković, T., Damjanović, A.,& Petrović, S.. (2018). Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 133, 225-232.
https://doi.org/10.1016/j.supflu.2017.10.021

Durdević S, Šavikin K, Živković JČ, Boehm V, Stanojković T, Damjanović A, Petrović S. Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids. 2018;133:225-232.
doi:10.1016/j.supflu.2017.10.021 .

Durdević, Sanja, Šavikin, Katarina, Živković, Jelena Č., Boehm, Volker, Stanojković, Tatjana, Damjanović, Ana, Petrović, Slobodan, "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." in Journal of Supercritical Fluids, 133 (2018):225-232,
https://doi.org/10.1016/j.supflu.2017.10.021 . .