TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4213
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
ER  - 

@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012"
}

Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012

Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012 .

Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4214
AB  - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP  - 390
SP  - 384
VL  - 213
DO  - 10.1016/j.saa.2019.01.069
ER  - 

@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069"
}

Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069

Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069 .

Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Uskoković-Marković, Snežana
AU  - Jelikić-Stankov, Milena
AU  - Radonjić, M.
AU  - Tanasković, D.
AU  - Lazarević, N.
AU  - Popović, Z. V.
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5779
AB  - We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectroscopy Letters
T1  - Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen
EP  - 305
SP  - 301
VL  - 126
DO  - 10.1016/j.saa.2014.01.135
ER  - 

@article{
author = "Lazarević, J. J. and Uskoković-Marković, Snežana and Jelikić-Stankov, Milena and Radonjić, M. and Tanasković, D. and Lazarević, N. and Popović, Z. V.",
year = "2014",
abstract = "We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectroscopy Letters",
title = "Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen",
pages = "305-301",
volume = "126",
doi = "10.1016/j.saa.2014.01.135"
}

Lazarević, J. J., Uskoković-Marković, S., Jelikić-Stankov, M., Radonjić, M., Tanasković, D., Lazarević, N.,& Popović, Z. V.. (2014). Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen. in Spectroscopy Letters
Pergamon-Elsevier Science Ltd, Oxford., 126, 301-305.
https://doi.org/10.1016/j.saa.2014.01.135

Lazarević JJ, Uskoković-Marković S, Jelikić-Stankov M, Radonjić M, Tanasković D, Lazarević N, Popović ZV. Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen. in Spectroscopy Letters. 2014;126:301-305.
doi:10.1016/j.saa.2014.01.135 .

Lazarević, J. J., Uskoković-Marković, Snežana, Jelikić-Stankov, Milena, Radonjić, M., Tanasković, D., Lazarević, N., Popović, Z. V., "Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen" in Spectroscopy Letters, 126 (2014):301-305,
https://doi.org/10.1016/j.saa.2014.01.135 . .