TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Ćosović, Vladan
AU  - Atanase, Leonard Ionut
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5866
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8  m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Ćosović, Vladan and Atanase, Leonard Ionut",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8  m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Ćosović, V.,& Atanase, L. I.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 24, 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Ćosović V, Atanase LI. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24:1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Ćosović, Vladan, Atanase, Leonard Ionut, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Stojanović, Zoran
AU  - Pekmezović, Marina
AU  - Veljović, Đorđe
AU  - O’connor, Kevin
AU  - Malagurski, Ivana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5104
AB  - Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.
PB  - MDPI
T2  - Pharmaceutics
T1  - Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model
IS  - 4
SP  - 696
VL  - 14
DO  - 10.3390/pharmaceutics14040696
ER  - 

@article{
author = "Pavić, Aleksandar and Stojanović, Zoran and Pekmezović, Marina and Veljović, Đorđe and O’connor, Kevin and Malagurski, Ivana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7–8 µm in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 × MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model",
number = "4",
pages = "696",
volume = "14",
doi = "10.3390/pharmaceutics14040696"
}

Pavić, A., Stojanović, Z., Pekmezović, M., Veljović, Đ., O’connor, K., Malagurski, I.,& Nikodinović-Runić, J.. (2022). Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics
MDPI., 14(4), 696.
https://doi.org/10.3390/pharmaceutics14040696

Pavić A, Stojanović Z, Pekmezović M, Veljović Đ, O’connor K, Malagurski I, Nikodinović-Runić J. Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics. 2022;14(4):696.
doi:10.3390/pharmaceutics14040696 .

Pavić, Aleksandar, Stojanović, Zoran, Pekmezović, Marina, Veljović, Đorđe, O’connor, Kevin, Malagurski, Ivana, Nikodinović-Runić, Jasmina, "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model" in Pharmaceutics, 14, no. 4 (2022):696,
https://doi.org/10.3390/pharmaceutics14040696 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Kalagasidis Krušić, Melina
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4826
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
T2  - Antibiotics
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
SP  - 737
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Kalagasidis Krušić, Melina and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
journal = "Antibiotics",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
pages = "737",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Kalagasidis Krušić, M., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics, 10(6), 737.
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Kalagasidis Krušić M, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics. 2021;10(6):737.
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Kalagasidis Krušić, Melina, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics, 10, no. 6 (2021):737,
https://doi.org/10.3390/antibiotics10060737 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan V.
AU  - Petrović, Jelena
AU  - Pavlović, Vladimir B.
AU  - Mitrić, Miodrag
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4054
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
EP  - 358
SP  - 352
VL  - 119
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan V. and Petrović, Jelena and Pavlović, Vladimir B. and Mitrić, Miodrag and Nešić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
pages = "358-352",
volume = "119",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M. V., Petrović, J., Pavlović, V. B., Mitrić, M., Nešić, A.,& Dimitrijević-Branković, S.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić MV, Petrović J, Pavlović VB, Mitrić M, Nešić A, Dimitrijević-Branković S. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan V., Petrović, Jelena, Pavlović, Vladimir B., Mitrić, Miodrag, Nešić, Aleksandra, Dimitrijević-Branković, Suzana, "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Kalušević, Ana
AU  - Nedović, Viktor
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4048
AB  - Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films
VL  - 255
DO  - 10.1016/j.matlet.2019.126572
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Kalušević, Ana and Nedović, Viktor and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films",
volume = "255",
doi = "10.1016/j.matlet.2019.126572"
}

Radovanović, N., Malagurski, I., Lević, S., Nešić, A., Cabrera-Barjas, G., Kalušević, A., Nedović, V., Pavlović, V.,& Dimitrijević-Branković, S.. (2019). Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters
Elsevier, Amsterdam., 255.
https://doi.org/10.1016/j.matlet.2019.126572

Radovanović N, Malagurski I, Lević S, Nešić A, Cabrera-Barjas G, Kalušević A, Nedović V, Pavlović V, Dimitrijević-Branković S. Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters. 2019;255.
doi:10.1016/j.matlet.2019.126572 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Kalušević, Ana, Nedović, Viktor, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films" in Materials Letters, 255 (2019),
https://doi.org/10.1016/j.matlet.2019.126572 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir B.
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3975
AB  - Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Letters
T1  - Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application
EP  - 36
SP  - 32
VL  - 212
DO  - 10.1016/j.matlet.2017.10.046
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Mitrić, Miodrag and Pavlović, Vladimir B. and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Letters",
title = "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application",
pages = "36-32",
volume = "212",
doi = "10.1016/j.matlet.2017.10.046"
}

Malagurski, I., Lević, S., Mitrić, M., Pavlović, V. B.,& Dimitrijević-Branković, S.. (2018). Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters
Elsevier Science Bv, Amsterdam., 212, 32-36.
https://doi.org/10.1016/j.matlet.2017.10.046

Malagurski I, Lević S, Mitrić M, Pavlović VB, Dimitrijević-Branković S. Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters. 2018;212:32-36.
doi:10.1016/j.matlet.2017.10.046 .

Malagurski, Ivana, Lević, Steva, Mitrić, Miodrag, Pavlović, Vladimir B., Dimitrijević-Branković, Suzana, "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application" in Materials Letters, 212 (2018):32-36,
https://doi.org/10.1016/j.matlet.2017.10.046 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nešić, Aleksandra
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir B.
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3539
AB  - New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties
EP  - 62
SP  - 55
VL  - 175
DO  - 10.1016/j.carbpol.2017.07.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Nešić, Aleksandra and Mitrić, Miodrag and Pavlović, Vladimir B. and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties",
pages = "62-55",
volume = "175",
doi = "10.1016/j.carbpol.2017.07.064"
}

Malagurski, I., Lević, S., Nešić, A., Mitrić, M., Pavlović, V. B.,& Dimitrijević-Branković, S.. (2017). Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 175, 55-62.
https://doi.org/10.1016/j.carbpol.2017.07.064

Malagurski I, Lević S, Nešić A, Mitrić M, Pavlović VB, Dimitrijević-Branković S. Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers. 2017;175:55-62.
doi:10.1016/j.carbpol.2017.07.064 .

Malagurski, Ivana, Lević, Steva, Nešić, Aleksandra, Mitrić, Miodrag, Pavlović, Vladimir B., Dimitrijević-Branković, Suzana, "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties" in Carbohydrate Polymers, 175 (2017):55-62,
https://doi.org/10.1016/j.carbpol.2017.07.064 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Matijasević, Danka
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir B.
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3686
AB  - New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites
EP  - 321
SP  - 313
VL  - 165
DO  - 10.1016/j.carbpol.2017.02.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Pantić, Milena and Matijasević, Danka and Mitrić, Miodrag and Pavlović, Vladimir B. and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites",
pages = "321-313",
volume = "165",
doi = "10.1016/j.carbpol.2017.02.064"
}

Malagurski, I., Lević, S., Pantić, M., Matijasević, D., Mitrić, M., Pavlović, V. B.,& Dimitrijević-Branković, S.. (2017). Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 165, 313-321.
https://doi.org/10.1016/j.carbpol.2017.02.064

Malagurski I, Lević S, Pantić M, Matijasević D, Mitrić M, Pavlović VB, Dimitrijević-Branković S. Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers. 2017;165:313-321.
doi:10.1016/j.carbpol.2017.02.064 .

Malagurski, Ivana, Lević, Steva, Pantić, Milena, Matijasević, Danka, Mitrić, Miodrag, Pavlović, Vladimir B., Dimitrijević-Branković, Suzana, "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites" in Carbohydrate Polymers, 165 (2017):313-321,
https://doi.org/10.1016/j.carbpol.2017.02.064 . .

TY  - JOUR
AU  - Kostić, Danijela
AU  - Malagurski, Ivana
AU  - Obradović, Bojana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3608
AB  - The aim of this work was to assess phenomena occurring during AgNP transport from nanocomposite Ag/alginate hydrogels under conditions relevant for potential biomedical applications as antimicrobial soft tissue implants. First, we have studied AgNP migration from the nanocomposite to the adjacent alginate hydrogel mimicking soft tissue next to the implant. AgNP deposition was carried out by the initial burst release lasting for similar to 24 h yielding large aggregates on hydrogel surfaces and smaller clusters (similar to 400 nm in size) inside. However, the overall released content was low (0.67%) indicating high nanocomposite stability. In the next experimental series, release of AgNPs, 10-30 nm in size, from Ag/alginate microbeads in water was investigated under static conditions as well as under continuous perfusion mimicking vascularized tissues. Mathematical modeling has revealed AgNP release by diffusion under static conditions with the diffusion coefficient within the Ag/alginate hydrogel of 6.9x10(-19) m(2) s(-1). Conversely, continuous perfusion induced increased AgNP release by convection with the interstitial fluid velocity estimated as 4.6 nm s(-1). Overall, the obtained results indicated the influence of hydrodynamic conditions at the implantation site on silver release and potential implant functionality, which should be investigated at the experimentation beginning using appropriate in vitro systems.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation
EP  - 394
IS  - 5
SP  - 383
VL  - 71
DO  - 10.2298/HEMIND160713049K
ER  - 

@article{
author = "Kostić, Danijela and Malagurski, Ivana and Obradović, Bojana",
year = "2017",
abstract = "The aim of this work was to assess phenomena occurring during AgNP transport from nanocomposite Ag/alginate hydrogels under conditions relevant for potential biomedical applications as antimicrobial soft tissue implants. First, we have studied AgNP migration from the nanocomposite to the adjacent alginate hydrogel mimicking soft tissue next to the implant. AgNP deposition was carried out by the initial burst release lasting for similar to 24 h yielding large aggregates on hydrogel surfaces and smaller clusters (similar to 400 nm in size) inside. However, the overall released content was low (0.67%) indicating high nanocomposite stability. In the next experimental series, release of AgNPs, 10-30 nm in size, from Ag/alginate microbeads in water was investigated under static conditions as well as under continuous perfusion mimicking vascularized tissues. Mathematical modeling has revealed AgNP release by diffusion under static conditions with the diffusion coefficient within the Ag/alginate hydrogel of 6.9x10(-19) m(2) s(-1). Conversely, continuous perfusion induced increased AgNP release by convection with the interstitial fluid velocity estimated as 4.6 nm s(-1). Overall, the obtained results indicated the influence of hydrodynamic conditions at the implantation site on silver release and potential implant functionality, which should be investigated at the experimentation beginning using appropriate in vitro systems.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation",
pages = "394-383",
number = "5",
volume = "71",
doi = "10.2298/HEMIND160713049K"
}

Kostić, D., Malagurski, I.,& Obradović, B.. (2017). Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 71(5), 383-394.
https://doi.org/10.2298/HEMIND160713049K

Kostić D, Malagurski I, Obradović B. Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation. in Hemijska industrija. 2017;71(5):383-394.
doi:10.2298/HEMIND160713049K .

Kostić, Danijela, Malagurski, Ivana, Obradović, Bojana, "Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation" in Hemijska industrija, 71, no. 5 (2017):383-394,
https://doi.org/10.2298/HEMIND160713049K . .

TY  - JOUR
AU  - Madžovska-Malagurski, Ivana
AU  - Vukašinović-Sekulić, Maja
AU  - Kostić, Danijela
AU  - Lević, Steva
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3283
AB  - The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.
PB  - IOP Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Towards antimicrobial yet bioactive Cu-alginate hydrogels
IS  - 3
VL  - 11
DO  - 10.1088/1748-6041/11/3/035015
ER  - 

@article{
author = "Madžovska-Malagurski, Ivana and Vukašinović-Sekulić, Maja and Kostić, Danijela and Lević, Steva",
year = "2016",
abstract = "The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Towards antimicrobial yet bioactive Cu-alginate hydrogels",
number = "3",
volume = "11",
doi = "10.1088/1748-6041/11/3/035015"
}

Madžovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D.,& Lević, S.. (2016). Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials
IOP Publishing Ltd, Bristol., 11(3).
https://doi.org/10.1088/1748-6041/11/3/035015

Madžovska-Malagurski I, Vukašinović-Sekulić M, Kostić D, Lević S. Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials. 2016;11(3).
doi:10.1088/1748-6041/11/3/035015 .

Madžovska-Malagurski, Ivana, Vukašinović-Sekulić, Maja, Kostić, Danijela, Lević, Steva, "Towards antimicrobial yet bioactive Cu-alginate hydrogels" in Biomedical Materials, 11, no. 3 (2016),
https://doi.org/10.1088/1748-6041/11/3/035015 . .