TY  - JOUR
AU  - Jokić, Bojan
AU  - Mitrić, Miodrag
AU  - Popović, Maja
AU  - Sima, L.
AU  - Petrescu, S. M.
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1863
AB  - In this work, the influence of the morphology of hydroxyapatite particles on silicon substitution through hydrothermal synthesis performed under the same conditions was investigated. Spherical- and whisker-like hydroxyapatite particles were obtained starting from calcium-nitrate, sodium dihydrogen phosphate, disodium-ethylenediaminetetraacetic acid and urea (used only for the synthesis of whisker-like particles) dissolved in aqueous solutions. Silicon was introduced into the solution using tetraethylorthosilicate. X-ray diffraction, infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy indicate that silicon doping induce different phase compositions and bioactivity of spherical- and whisker-like hydroxyapatite particles obtained under the same hydrothermal conditions. Silicon-substituted, spherical hydroxyapatites particles showed greater phase transformation to silicon-substituted alpha- calcium-phosphate compared with whiskers-like hydroxyapatite particles synthesized with the same amount of added silicon. Metabolic activity assay performed with SaOs2 osteosarcoma cells showed better biocompatibility of annealed biphasic spherical-like particles compared with annealed whiskerlike particles while dried spherical-like particles induce high cytotoxicity effect.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - The influence of silicon substitution on the properties of spherical- and whisker-like biphasic alpha-calcium-phosphate/hydroxyapatite particles
EP  - 2185
IS  - 10
SP  - 2175
VL  - 22
DO  - 10.1007/s10856-011-4412-6
ER  - 

@article{
author = "Jokić, Bojan and Mitrić, Miodrag and Popović, Maja and Sima, L. and Petrescu, S. M. and Petrović, Rada and Janaćković, Đorđe",
year = "2011",
abstract = "In this work, the influence of the morphology of hydroxyapatite particles on silicon substitution through hydrothermal synthesis performed under the same conditions was investigated. Spherical- and whisker-like hydroxyapatite particles were obtained starting from calcium-nitrate, sodium dihydrogen phosphate, disodium-ethylenediaminetetraacetic acid and urea (used only for the synthesis of whisker-like particles) dissolved in aqueous solutions. Silicon was introduced into the solution using tetraethylorthosilicate. X-ray diffraction, infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy indicate that silicon doping induce different phase compositions and bioactivity of spherical- and whisker-like hydroxyapatite particles obtained under the same hydrothermal conditions. Silicon-substituted, spherical hydroxyapatites particles showed greater phase transformation to silicon-substituted alpha- calcium-phosphate compared with whiskers-like hydroxyapatite particles synthesized with the same amount of added silicon. Metabolic activity assay performed with SaOs2 osteosarcoma cells showed better biocompatibility of annealed biphasic spherical-like particles compared with annealed whiskerlike particles while dried spherical-like particles induce high cytotoxicity effect.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "The influence of silicon substitution on the properties of spherical- and whisker-like biphasic alpha-calcium-phosphate/hydroxyapatite particles",
pages = "2185-2175",
number = "10",
volume = "22",
doi = "10.1007/s10856-011-4412-6"
}

Jokić, B., Mitrić, M., Popović, M., Sima, L., Petrescu, S. M., Petrović, R.,& Janaćković, Đ.. (2011). The influence of silicon substitution on the properties of spherical- and whisker-like biphasic alpha-calcium-phosphate/hydroxyapatite particles. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 22(10), 2175-2185.
https://doi.org/10.1007/s10856-011-4412-6

Jokić B, Mitrić M, Popović M, Sima L, Petrescu SM, Petrović R, Janaćković Đ. The influence of silicon substitution on the properties of spherical- and whisker-like biphasic alpha-calcium-phosphate/hydroxyapatite particles. in Journal of Materials Science-Materials in Medicine. 2011;22(10):2175-2185.
doi:10.1007/s10856-011-4412-6 .

Jokić, Bojan, Mitrić, Miodrag, Popović, Maja, Sima, L., Petrescu, S. M., Petrović, Rada, Janaćković, Đorđe, "The influence of silicon substitution on the properties of spherical- and whisker-like biphasic alpha-calcium-phosphate/hydroxyapatite particles" in Journal of Materials Science-Materials in Medicine, 22, no. 10 (2011):2175-2185,
https://doi.org/10.1007/s10856-011-4412-6 . .

TY  - JOUR
AU  - Popescu, A. C.
AU  - Sima, F.
AU  - Duta, L.
AU  - Popescu, A. C.
AU  - Mihailescu, Ion N.
AU  - Capitanu, D.
AU  - Mustata, R.
AU  - Sima, L. E.
AU  - Petrescu, S. M.
AU  - Janaćković, Đorđe
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1483
AB  - We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests
EP  - 5490
IS  - 10
SP  - 5486
VL  - 255
DO  - 10.1016/j.apsusc.2008.10.031
ER  - 

@article{
author = "Popescu, A. C. and Sima, F. and Duta, L. and Popescu, A. C. and Mihailescu, Ion N. and Capitanu, D. and Mustata, R. and Sima, L. E. and Petrescu, S. M. and Janaćković, Đorđe",
year = "2009",
abstract = "We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests",
pages = "5490-5486",
number = "10",
volume = "255",
doi = "10.1016/j.apsusc.2008.10.031"
}

Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, I. N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M.,& Janaćković, Đ.. (2009). Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science
Elsevier, Amsterdam., 255(10), 5486-5490.
https://doi.org/10.1016/j.apsusc.2008.10.031

Popescu AC, Sima F, Duta L, Popescu AC, Mihailescu IN, Capitanu D, Mustata R, Sima LE, Petrescu SM, Janaćković Đ. Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science. 2009;255(10):5486-5490.
doi:10.1016/j.apsusc.2008.10.031 .

Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, Ion N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M., Janaćković, Đorđe, "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests" in Applied Surface Science, 255, no. 10 (2009):5486-5490,
https://doi.org/10.1016/j.apsusc.2008.10.031 . .

TY  - JOUR
AU  - Floroian, L.
AU  - Savu, B.
AU  - Stanciu, G.
AU  - Popescu, A. C.
AU  - Sima, F.
AU  - Mihailescu, Ion N.
AU  - Mustata, R.
AU  - Sima, L. E.
AU  - Petrescu, M.
AU  - Tanasković, Dušanka
AU  - Janaćković, Đorđe
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1230
AB  - We report the synthesis by pulsed laser deposition of thin structures of two bioactive glasses belonging to the SiO2-Na2O-K2O-CaO-MgO-P2O5 system, on medical grade Ti substrates. We evaluated their biocompatibility after immersion in simulated body fluids and by performing cells adhesion tests. The Films were characterized by confocal scanning laser microscopy and Fourier transform infrared spectrometry, before and after 30 and 46 days immersion in fluids. Our studies demonstrated that deposited coatings were degraded in simulated fluids. A new apatite layer was synthesized by ions changing with the fluid during the decomposition of bioglasses. We investigated after immersion in fluids cells adhesion and the cytoskelet organization of synthesized structures, by fluorescence microscopy. A good adhesion to bioglass coatings was evidenced.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests
EP  - 3062
IS  - 5
SP  - 3056
VL  - 255
DO  - 10.1016/j.apsusc.2008.08.105
ER  - 

@article{
author = "Floroian, L. and Savu, B. and Stanciu, G. and Popescu, A. C. and Sima, F. and Mihailescu, Ion N. and Mustata, R. and Sima, L. E. and Petrescu, M. and Tanasković, Dušanka and Janaćković, Đorđe",
year = "2008",
abstract = "We report the synthesis by pulsed laser deposition of thin structures of two bioactive glasses belonging to the SiO2-Na2O-K2O-CaO-MgO-P2O5 system, on medical grade Ti substrates. We evaluated their biocompatibility after immersion in simulated body fluids and by performing cells adhesion tests. The Films were characterized by confocal scanning laser microscopy and Fourier transform infrared spectrometry, before and after 30 and 46 days immersion in fluids. Our studies demonstrated that deposited coatings were degraded in simulated fluids. A new apatite layer was synthesized by ions changing with the fluid during the decomposition of bioglasses. We investigated after immersion in fluids cells adhesion and the cytoskelet organization of synthesized structures, by fluorescence microscopy. A good adhesion to bioglass coatings was evidenced.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests",
pages = "3062-3056",
number = "5",
volume = "255",
doi = "10.1016/j.apsusc.2008.08.105"
}

Floroian, L., Savu, B., Stanciu, G., Popescu, A. C., Sima, F., Mihailescu, I. N., Mustata, R., Sima, L. E., Petrescu, M., Tanasković, D.,& Janaćković, Đ.. (2008). Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests. in Applied Surface Science
Elsevier, Amsterdam., 255(5), 3056-3062.
https://doi.org/10.1016/j.apsusc.2008.08.105

Floroian L, Savu B, Stanciu G, Popescu AC, Sima F, Mihailescu IN, Mustata R, Sima LE, Petrescu M, Tanasković D, Janaćković Đ. Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests. in Applied Surface Science. 2008;255(5):3056-3062.
doi:10.1016/j.apsusc.2008.08.105 .

Floroian, L., Savu, B., Stanciu, G., Popescu, A. C., Sima, F., Mihailescu, Ion N., Mustata, R., Sima, L. E., Petrescu, M., Tanasković, Dušanka, Janaćković, Đorđe, "Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests" in Applied Surface Science, 255, no. 5 (2008):3056-3062,
https://doi.org/10.1016/j.apsusc.2008.08.105 . .

TY  - JOUR
AU  - Gyorgy, E.
AU  - Grigorescu, S.
AU  - Socol, Gabriel
AU  - Mihailescu, Ion N.
AU  - Janaćković, Đorđe
AU  - Dindune, Antonija
AU  - Kanepe, Z.
AU  - Palcevskis, Eriks
AU  - Zdrentu, E. L.
AU  - Petrescu, S. M.
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1170
AB  - Bioactive glass (BG), calcium hydroxyapatite (HA), and ZrO2 doped HA thin films were grown by pulsed laser deposition on Ti substrates. An UV KrF* (lambda = 248 nm, tau  gt = 7 ns) excimer laser was used for the multi-pulse irradiation of the targets. The substrates were kept at room temperature or heated during the film deposition at values within the (400-550 degrees C) range. The depositions were performed in oxygen and water vapor atmospheres, at pressure values in the range (5-40 Pa). The HA coatings were heat post-treated for 6 h in a flux of hot water vapors at the same temperature as applied during deposition. The surface morphology, chemical composition, and crystalline quality of the obtained thin films were studied by scanning electron microscopy, atomic force microscopy, and X-ray diffractometry. The films were seeded for in vitro tests with Hek293 (human embryonic kidney) cells that revealed a good adherence on the deposited layers. Biocompatibility tests showed that cell growth was better on HA than on BG thin films.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Bioactive glass and hydroxyapatite thin films obtained by pulsed laser deposition
EP  - 7986
IS  - 19
SP  - 7981
VL  - 253
DO  - 10.1016/j.apsusc.2007.02.146
ER  - 

@article{
author = "Gyorgy, E. and Grigorescu, S. and Socol, Gabriel and Mihailescu, Ion N. and Janaćković, Đorđe and Dindune, Antonija and Kanepe, Z. and Palcevskis, Eriks and Zdrentu, E. L. and Petrescu, S. M.",
year = "2007",
abstract = "Bioactive glass (BG), calcium hydroxyapatite (HA), and ZrO2 doped HA thin films were grown by pulsed laser deposition on Ti substrates. An UV KrF* (lambda = 248 nm, tau  gt = 7 ns) excimer laser was used for the multi-pulse irradiation of the targets. The substrates were kept at room temperature or heated during the film deposition at values within the (400-550 degrees C) range. The depositions were performed in oxygen and water vapor atmospheres, at pressure values in the range (5-40 Pa). The HA coatings were heat post-treated for 6 h in a flux of hot water vapors at the same temperature as applied during deposition. The surface morphology, chemical composition, and crystalline quality of the obtained thin films were studied by scanning electron microscopy, atomic force microscopy, and X-ray diffractometry. The films were seeded for in vitro tests with Hek293 (human embryonic kidney) cells that revealed a good adherence on the deposited layers. Biocompatibility tests showed that cell growth was better on HA than on BG thin films.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Bioactive glass and hydroxyapatite thin films obtained by pulsed laser deposition",
pages = "7986-7981",
number = "19",
volume = "253",
doi = "10.1016/j.apsusc.2007.02.146"
}

Gyorgy, E., Grigorescu, S., Socol, G., Mihailescu, I. N., Janaćković, Đ., Dindune, A., Kanepe, Z., Palcevskis, E., Zdrentu, E. L.,& Petrescu, S. M.. (2007). Bioactive glass and hydroxyapatite thin films obtained by pulsed laser deposition. in Applied Surface Science
Elsevier, Amsterdam., 253(19), 7981-7986.
https://doi.org/10.1016/j.apsusc.2007.02.146

Gyorgy E, Grigorescu S, Socol G, Mihailescu IN, Janaćković Đ, Dindune A, Kanepe Z, Palcevskis E, Zdrentu EL, Petrescu SM. Bioactive glass and hydroxyapatite thin films obtained by pulsed laser deposition. in Applied Surface Science. 2007;253(19):7981-7986.
doi:10.1016/j.apsusc.2007.02.146 .

Gyorgy, E., Grigorescu, S., Socol, Gabriel, Mihailescu, Ion N., Janaćković, Đorđe, Dindune, Antonija, Kanepe, Z., Palcevskis, Eriks, Zdrentu, E. L., Petrescu, S. M., "Bioactive glass and hydroxyapatite thin films obtained by pulsed laser deposition" in Applied Surface Science, 253, no. 19 (2007):7981-7986,
https://doi.org/10.1016/j.apsusc.2007.02.146 . .