TY  - JOUR
AU  - Katić, Katarina
AU  - Banjanac, Katarina
AU  - Simović, Milica
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4864
AB  - In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides,   lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.
PB  - John Wiley and Sons Inc.
T2  - International Journal of Food Science and Technology
T1  - Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate
EP  - 4297
IS  - 9
SP  - 4287
VL  - 56
DO  - 10.1111/ijfs.15189
ER  - 

@article{
author = "Katić, Katarina and Banjanac, Katarina and Simović, Milica and Ćorović, Marija and Milivojević, Ana and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2021",
abstract = "In this study, the suitability of fumed silica nanoparticles (FNS) and its derivatives (amino-modified FNS (AFNS), cyanuric chloride-activated AFNS (CCAFNS) and epoxy-modified FNS (GFNS)), for covalent immobilisation of two commercial protease preparations Alcalase(R) and Flavourzyme(R) was investigated. The highest hydrolytic activities of immobilised preparations were 25 IU g(-1) support (Alcalase-GFNS) and 2.95 IU g(-1) support (Flavourzyme-CCAFNS). Furthermore, the immobilised preparations showed 43% and 20% of initial specific activities of commercial protease preparations, respectively. Flavourzyme-CCAFNS also exhibited the highest exopeptidase activity of 22.83 L-pNAU g(-1) support. Finally, these two nanobiocatalysts were successfully applied for hydrolysis of sunflower meal protein isolate (SMPI), providing two times higher hydrolysis yields in comparison to free enzymes, justifying the applied immobilisation process. Namely, the highest hydrolysis yield (30%) was gained by the sequential hydrolysis with Alcalase-GFNS and Flavourzyme-CCAFNS, which resulted in the formation of small hydrophobic and hydrophilic peptides,   lt = 5 kDa, confirmed by HPLC analysis and electrophoretic separation.",
publisher = "John Wiley and Sons Inc.",
journal = "International Journal of Food Science and Technology",
title = "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate",
pages = "4297-4287",
number = "9",
volume = "56",
doi = "10.1111/ijfs.15189"
}

Katić, K., Banjanac, K., Simović, M., Ćorović, M., Milivojević, A., Marinković, A.,& Bezbradica, D.. (2021). Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology
John Wiley and Sons Inc.., 56(9), 4287-4297.
https://doi.org/10.1111/ijfs.15189

Katić K, Banjanac K, Simović M, Ćorović M, Milivojević A, Marinković A, Bezbradica D. Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate. in International Journal of Food Science and Technology. 2021;56(9):4287-4297.
doi:10.1111/ijfs.15189 .

Katić, Katarina, Banjanac, Katarina, Simović, Milica, Ćorović, Marija, Milivojević, Ana, Marinković, Aleksandar, Bezbradica, Dejan, "Development of protease nanobiocatalysts and their application in hydrolysis of sunflower meal protein isolate" in International Journal of Food Science and Technology, 56, no. 9 (2021):4287-4297,
https://doi.org/10.1111/ijfs.15189 . .