@conference{
author = "Mijalković, Jelena and Šekuljica, Nataša and Biçer, Furkan and Pavlović, Neda and Jakovetić Tanasković, Sonja and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Proteins are an essential component of the human diet and effectively are provided from
both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry,
is a promising tool for obtaining protein with an excellent nutritional profile under the
green biorafinery concept, that can be used as a substitute for animal proteins. This study
provides the results regarding the implementation of ultrasound-assisted processing (UAP)
in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was
optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and
time. UAP appears to have a strong potential for recovering RuBisCO protein from
pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP
amplitude and time., Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema
konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat
karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije
prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom
vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom
radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća
bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem
odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka.
Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća
bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.",
publisher = "Beograd : Srpsko hemijsko društvo = Serbian Chemical Society",
journal = "Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade",
title = "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6252"
}