Gasic, Uros M.

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Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Kolarevic, Tijana; Milincic, Danijel D.; Vujovic, Tatjana; Gasic, Uros M.; Prokic, Ljiljana; Kostic, Aleksandar Z.; Cerović, Radosav; Stanojevic, Sladjana P.; Tesic, Zivoslav Lj.; Pesic, Mirjana B.

(2021)

TY  - JOUR
AU  - Kolarevic, Tijana
AU  - Milincic, Danijel D.
AU  - Vujovic, Tatjana
AU  - Gasic, Uros M.
AU  - Prokic, Ljiljana
AU  - Kostic, Aleksandar Z.
AU  - Cerović, Radosav
AU  - Stanojevic, Sladjana P.
AU  - Tesic, Zivoslav Lj.
AU  - Pesic, Mirjana B.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4814
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry 'Cacanska Bestrna' and blueberry 'Toro'. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA(3) ('Cacanska Bestrna') or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA ('Toro'). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D ('Cacanska Bestrna') or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA ('Toro'). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS(& BULL;+) and DPPH & BULL; scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS(& BULL;+) and ten to seventeen times lower DPPH & BULL; scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
VL  - 7
DO  - 10.3390/horticulturae7110420
ER  - 
@article{
author = "Kolarevic, Tijana and Milincic, Danijel D. and Vujovic, Tatjana and Gasic, Uros M. and Prokic, Ljiljana and Kostic, Aleksandar Z. and Cerović, Radosav and Stanojevic, Sladjana P. and Tesic, Zivoslav Lj. and Pesic, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry 'Cacanska Bestrna' and blueberry 'Toro'. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA(3) ('Cacanska Bestrna') or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA ('Toro'). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D ('Cacanska Bestrna') or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA ('Toro'). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS(& BULL;+) and DPPH & BULL; scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS(& BULL;+) and ten to seventeen times lower DPPH & BULL; scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
volume = "7",
doi = "10.3390/horticulturae7110420"
}
Kolarevic, T., Milincic, D. D., Vujovic, T., Gasic, U. M., Prokic, L., Kostic, A. Z., Cerović, R., Stanojevic, S. P., Tesic, Z. Lj.,& Pesic, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae, 7(11).
https://doi.org/10.3390/horticulturae7110420
Kolarevic T, Milincic DD, Vujovic T, Gasic UM, Prokic L, Kostic AZ, Cerović R, Stanojevic SP, Tesic ZL, Pesic MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11).
doi:10.3390/horticulturae7110420 .
Kolarevic, Tijana, Milincic, Danijel D., Vujovic, Tatjana, Gasic, Uros M., Prokic, Ljiljana, Kostic, Aleksandar Z., Cerović, Radosav, Stanojevic, Sladjana P., Tesic, Zivoslav Lj., Pesic, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021),
https://doi.org/10.3390/horticulturae7110420 . .
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