TY  - JOUR
AU  - Sajc, L.
AU  - Vunjak-Novaković, Gordana
AU  - Grubisić, D.
AU  - Kovačević, N.
AU  - Vuković, Dragan
AU  - Bugarski, Branko
PY  - 1995
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/60
AB  - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
EP  - 423
IS  - 3
SP  - 416
VL  - 43
DO  - 10.1007/BF00218443
ER  - 

@article{
author = "Sajc, L. and Vunjak-Novaković, Gordana and Grubisić, D. and Kovačević, N. and Vuković, Dragan and Bugarski, Branko",
year = "1995",
abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor",
pages = "423-416",
number = "3",
volume = "43",
doi = "10.1007/BF00218443"
}

Sajc, L., Vunjak-Novaković, G., Grubisić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology
Springer, New York., 43(3), 416-423.
https://doi.org/10.1007/BF00218443

Sajc L, Vunjak-Novaković G, Grubisić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423.
doi:10.1007/BF00218443 .

Sajc, L., Vunjak-Novaković, Gordana, Grubisić, D., Kovačević, N., Vuković, Dragan, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423,
https://doi.org/10.1007/BF00218443 . .

TY  - JOUR
AU  - Sajc, L.
AU  - Obradović, Bojana
AU  - Vuković, Dragan
AU  - Bugarski, Branko
AU  - Grubisić, D.
AU  - Vunjak-Novaković, Gordana
PY  - 1995
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/59
AB  - An external loop air lift reactor (ELALR) was developed and designed for continuous production and extraction of extracellular secondary metabolites by immobilized Frangula alnus Mill. plant cells. This system was considered as a four‐phase system, with dispersed gas, nonaqueous liquid solvent, and calcium alginate particles in water or Murashige and Skoog medium. Analysis of the four‐phase ELALR revealed the influence of gas, solvent, calcium alginate particles and MS medium composition on gas and solvent holdup, liquid circulation velocity, mixing in aqueous liquid phase, and mass transfer coefficients of oxygen and plant cell product. It was shown that the gas holdup appears to be the principal variable determining the properties of the four‐phase system. An unified correlation for the gas holdup, based on drift flux flow in a three‐phase system, has been extended to the four‐phase system. Bubble size and bubble size distribution observed in the bubbly flow regime were the same in water as in the MS medium system (Sauter mean diameter 2.5 mm). Consequently, gas holdup and all related parameters, such as liquid circulation velocity, oxygen mass transfer coefficient, and axial liquid dispersion in MS medium, were similar to those in water for the four‐phase system.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Hydrodynamics and Mass Transfer in a Four‐Phase External Loop Air Lift Bioreactor
EP  - 428
IS  - 4
SP  - 420
VL  - 11
DO  - 10.1021/bp00034a009
ER  - 

@article{
author = "Sajc, L. and Obradović, Bojana and Vuković, Dragan and Bugarski, Branko and Grubisić, D. and Vunjak-Novaković, Gordana",
year = "1995",
abstract = "An external loop air lift reactor (ELALR) was developed and designed for continuous production and extraction of extracellular secondary metabolites by immobilized Frangula alnus Mill. plant cells. This system was considered as a four‐phase system, with dispersed gas, nonaqueous liquid solvent, and calcium alginate particles in water or Murashige and Skoog medium. Analysis of the four‐phase ELALR revealed the influence of gas, solvent, calcium alginate particles and MS medium composition on gas and solvent holdup, liquid circulation velocity, mixing in aqueous liquid phase, and mass transfer coefficients of oxygen and plant cell product. It was shown that the gas holdup appears to be the principal variable determining the properties of the four‐phase system. An unified correlation for the gas holdup, based on drift flux flow in a three‐phase system, has been extended to the four‐phase system. Bubble size and bubble size distribution observed in the bubbly flow regime were the same in water as in the MS medium system (Sauter mean diameter 2.5 mm). Consequently, gas holdup and all related parameters, such as liquid circulation velocity, oxygen mass transfer coefficient, and axial liquid dispersion in MS medium, were similar to those in water for the four‐phase system.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Hydrodynamics and Mass Transfer in a Four‐Phase External Loop Air Lift Bioreactor",
pages = "428-420",
number = "4",
volume = "11",
doi = "10.1021/bp00034a009"
}

Sajc, L., Obradović, B., Vuković, D., Bugarski, B., Grubisić, D.,& Vunjak-Novaković, G.. (1995). Hydrodynamics and Mass Transfer in a Four‐Phase External Loop Air Lift Bioreactor. in Biotechnology Progress
Wiley, Hoboken., 11(4), 420-428.
https://doi.org/10.1021/bp00034a009

Sajc L, Obradović B, Vuković D, Bugarski B, Grubisić D, Vunjak-Novaković G. Hydrodynamics and Mass Transfer in a Four‐Phase External Loop Air Lift Bioreactor. in Biotechnology Progress. 1995;11(4):420-428.
doi:10.1021/bp00034a009 .

Sajc, L., Obradović, Bojana, Vuković, Dragan, Bugarski, Branko, Grubisić, D., Vunjak-Novaković, Gordana, "Hydrodynamics and Mass Transfer in a Four‐Phase External Loop Air Lift Bioreactor" in Biotechnology Progress, 11, no. 4 (1995):420-428,
https://doi.org/10.1021/bp00034a009 . .