TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Mutavdžić, Dragosav
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6635
AB  - In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.
PB  - John Wiley and Sons Inc
T2  - Biotechnology Journal
T1  - Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions
DO  - 10.1002/biot.202300312
ER  - 

@article{
author = "Spasojević, Dragica and Prodanović, Olivera and Mutavdžić, Dragosav and Šekuljica, Nataša and Jovanović, Jelena and Maksimović, Vuk and Radotić, Ksenija",
year = "2023",
abstract = "In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.",
publisher = "John Wiley and Sons Inc",
journal = "Biotechnology Journal",
title = "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions",
doi = "10.1002/biot.202300312"
}

Spasojević, D., Prodanović, O., Mutavdžić, D., Šekuljica, N., Jovanović, J., Maksimović, V.,& Radotić, K.. (2023). Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal
John Wiley and Sons Inc..
https://doi.org/10.1002/biot.202300312

Spasojević D, Prodanović O, Mutavdžić D, Šekuljica N, Jovanović J, Maksimović V, Radotić K. Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal. 2023;.
doi:10.1002/biot.202300312 .

Spasojević, Dragica, Prodanović, Olivera, Mutavdžić, Dragosav, Šekuljica, Nataša, Jovanović, Jelena, Maksimović, Vuk, Radotić, Ksenija, "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions" in Biotechnology Journal (2023),
https://doi.org/10.1002/biot.202300312 . .

TY  - JOUR
AU  - Marković, Jelena M.
AU  - Trišović, Nemanja
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Juranić, Ivan
AU  - Drakulić, Branko
AU  - Marinković, Aleksandar
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3026
AB  - Seven symmetrical 2,6-distyrylpyridines, phenyl-substituted with hydrogen-bond donors, hydrogen-bond acceptors, halogens and hydrophobic moieties were synthesized and their spectroscopic characterization was done. Solvent effects on the absorption and fluorescence spectra were analyzed and quantified using the Kamlet-Taft and Catalan approach. The obtained results were rationalized by comparison of electrostatic potentials of the molecules in the ground and in excited state and by comparison of the frontier molecular orbitals (HOMO and LUMO), derived from quantum-mechanical calculations (HE, DFT, MP2). Analysis of the results revealed an important influence of non-specific (dispersive) interactions on the solvatochromic behavior of the compounds. 1D and 2D NMR data, in silica obtained conformational assembly of the compound, and the NMR analysis of molecular flexibility in solution (NAMFIS), were used to estimate population of conformers and to deconvolute the UV-Vis spectrum of representative derivative; inferring that the conformational assembly is more complex than was assumed in so far published literature data for this class of compounds. Along with this, the emission spectra of the representative compounds were decomposed by the Multivariate Curve Resolution analysis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study
EP  - 446
SP  - 435
VL  - 135
DO  - 10.1016/j.saa.2014.07.023
ER  - 

@article{
author = "Marković, Jelena M. and Trišović, Nemanja and Mutavdžić, Dragosav and Radotić, Ksenija and Juranić, Ivan and Drakulić, Branko and Marinković, Aleksandar",
year = "2015",
abstract = "Seven symmetrical 2,6-distyrylpyridines, phenyl-substituted with hydrogen-bond donors, hydrogen-bond acceptors, halogens and hydrophobic moieties were synthesized and their spectroscopic characterization was done. Solvent effects on the absorption and fluorescence spectra were analyzed and quantified using the Kamlet-Taft and Catalan approach. The obtained results were rationalized by comparison of electrostatic potentials of the molecules in the ground and in excited state and by comparison of the frontier molecular orbitals (HOMO and LUMO), derived from quantum-mechanical calculations (HE, DFT, MP2). Analysis of the results revealed an important influence of non-specific (dispersive) interactions on the solvatochromic behavior of the compounds. 1D and 2D NMR data, in silica obtained conformational assembly of the compound, and the NMR analysis of molecular flexibility in solution (NAMFIS), were used to estimate population of conformers and to deconvolute the UV-Vis spectrum of representative derivative; inferring that the conformational assembly is more complex than was assumed in so far published literature data for this class of compounds. Along with this, the emission spectra of the representative compounds were decomposed by the Multivariate Curve Resolution analysis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study",
pages = "446-435",
volume = "135",
doi = "10.1016/j.saa.2014.07.023"
}

Marković, J. M., Trišović, N., Mutavdžić, D., Radotić, K., Juranić, I., Drakulić, B.,& Marinković, A.. (2015). Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 135, 435-446.
https://doi.org/10.1016/j.saa.2014.07.023

Marković JM, Trišović N, Mutavdžić D, Radotić K, Juranić I, Drakulić B, Marinković A. Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2015;135:435-446.
doi:10.1016/j.saa.2014.07.023 .

Marković, Jelena M., Trišović, Nemanja, Mutavdžić, Dragosav, Radotić, Ksenija, Juranić, Ivan, Drakulić, Branko, Marinković, Aleksandar, "Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 135 (2015):435-446,
https://doi.org/10.1016/j.saa.2014.07.023 . .

TY  - JOUR
AU  - Lađarević, Jelena
AU  - Božić, Bojan
AU  - Mutavdžić, Dragosav
AU  - Ušćumlić, Gordana
AU  - Mijin, Dušan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2750
AB  - Spectral properties, solvatochromism and azo-hydrazone tautomerism of ten 5-(substituted phenylazo)3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones in twenty-two solvents are investigated. For quantitative evaluation of the solvent effects on the UV-vis absorption maxima, the principles of the linear solvation energy relationships are used, i.e. models proposed by Kamlet-Taft and Catalan. Linear free energy relationships are applied to the UV-vis absorption spectra and correlation of absorption frequencies with Hammett substituent constants are performed. Furthermore, the influence of the electronic nature of the substituents on H-1 and C-13 NMR shifts is investigated by simple and extended Hammett equations, as well as by Swain-Lupton equation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Chemical Physics Letters
T1  - Solvent and structural effects on the spectral shifts of 5-(substituted phenylazo)-3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones
EP  - 68
SP  - 62
VL  - 615
DO  - 10.1016/j.cplett.2014.09.063
ER  - 

@article{
author = "Lađarević, Jelena and Božić, Bojan and Mutavdžić, Dragosav and Ušćumlić, Gordana and Mijin, Dušan",
year = "2014",
abstract = "Spectral properties, solvatochromism and azo-hydrazone tautomerism of ten 5-(substituted phenylazo)3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones in twenty-two solvents are investigated. For quantitative evaluation of the solvent effects on the UV-vis absorption maxima, the principles of the linear solvation energy relationships are used, i.e. models proposed by Kamlet-Taft and Catalan. Linear free energy relationships are applied to the UV-vis absorption spectra and correlation of absorption frequencies with Hammett substituent constants are performed. Furthermore, the influence of the electronic nature of the substituents on H-1 and C-13 NMR shifts is investigated by simple and extended Hammett equations, as well as by Swain-Lupton equation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Chemical Physics Letters",
title = "Solvent and structural effects on the spectral shifts of 5-(substituted phenylazo)-3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones",
pages = "68-62",
volume = "615",
doi = "10.1016/j.cplett.2014.09.063"
}

Lađarević, J., Božić, B., Mutavdžić, D., Ušćumlić, G.,& Mijin, D.. (2014). Solvent and structural effects on the spectral shifts of 5-(substituted phenylazo)-3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones. in Chemical Physics Letters
Elsevier Science Bv, Amsterdam., 615, 62-68.
https://doi.org/10.1016/j.cplett.2014.09.063

Lađarević J, Božić B, Mutavdžić D, Ušćumlić G, Mijin D. Solvent and structural effects on the spectral shifts of 5-(substituted phenylazo)-3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones. in Chemical Physics Letters. 2014;615:62-68.
doi:10.1016/j.cplett.2014.09.063 .

Lađarević, Jelena, Božić, Bojan, Mutavdžić, Dragosav, Ušćumlić, Gordana, Mijin, Dušan, "Solvent and structural effects on the spectral shifts of 5-(substituted phenylazo)-3-cyano-6-hydroxy-1-(2-hydroxyethyl)-4-methyl-2-pyridones" in Chemical Physics Letters, 615 (2014):62-68,
https://doi.org/10.1016/j.cplett.2014.09.063 . .