TY  - JOUR
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Grzęda, Anita
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6031
AB  - The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.
PB  - Springer
T2  - Food and Bioprocess Technology
T1  - A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds
DO  - 10.1007/s11947-023-03038-9
ER  - 

@article{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Grzęda, Anita and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.",
publisher = "Springer",
journal = "Food and Bioprocess Technology",
title = "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds",
doi = "10.1007/s11947-023-03038-9"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Grzęda, A., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2023). A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology
Springer..
https://doi.org/10.1007/s11947-023-03038-9

Milovanović S, Grzegorczyk A, Świątek Ł, Grzęda A, Dębczak A, Tyskiewicz K, Konkol M. A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology. 2023;.
doi:10.1007/s11947-023-03038-9 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Grzęda, Anita, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds" in Food and Bioprocess Technology (2023),
https://doi.org/10.1007/s11947-023-03038-9 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Kaminski, Piotr
AU  - Debczak, Agnieszka
AU  - Klimkowska, Kamila
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5190
AB  - The aim of this study was to provide insights into parameters that affect the oil extraction from an industrially valuable raw material (milk thistle) using green solvent supercritical CO2. The effect of plant origin, cultivation year, and seeds storage on extraction yield has been explored. Furthermore, the effects of high-pressure unit design and initial plant material amount have been examined. Pressure (200-450 bar) and temperature (40-80 °C) have been tested to improve the recovery of essential fatty acids and phenolic compounds. Moreover, solid residues after the material pretreatment and supercritical CO2 extraction served as the substrates for waste valorization. The characterization of obtained high-value extracts has been carried out by chromatographic (using gas chromatographs combined with mass spectrometer and flame ionization detection) and spectrophotometric assays (for total phenolic and flavonoid compounds as well as a radical scavenging ability). It was shown that the tested supercritical CO2 extraction process parameters determined extraction kinetics and allowed the extract separation from raw milk thistle seeds and waste material (3-32 mass %) rich in linoleic acid (up to 515 mg/g) and total phenolic compounds (up to 30 mg GAE/g).
PB  - Elsevier Ltd
T2  - Journal of CO2 Utilization
T1  - Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process
SP  - 102134
VL  - 63
DO  - 10.1016/j.jcou.2022.102134
ER  - 

@article{
author = "Milovanović, Stoja and Lukić, Ivana and Kaminski, Piotr and Debczak, Agnieszka and Klimkowska, Kamila and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The aim of this study was to provide insights into parameters that affect the oil extraction from an industrially valuable raw material (milk thistle) using green solvent supercritical CO2. The effect of plant origin, cultivation year, and seeds storage on extraction yield has been explored. Furthermore, the effects of high-pressure unit design and initial plant material amount have been examined. Pressure (200-450 bar) and temperature (40-80 °C) have been tested to improve the recovery of essential fatty acids and phenolic compounds. Moreover, solid residues after the material pretreatment and supercritical CO2 extraction served as the substrates for waste valorization. The characterization of obtained high-value extracts has been carried out by chromatographic (using gas chromatographs combined with mass spectrometer and flame ionization detection) and spectrophotometric assays (for total phenolic and flavonoid compounds as well as a radical scavenging ability). It was shown that the tested supercritical CO2 extraction process parameters determined extraction kinetics and allowed the extract separation from raw milk thistle seeds and waste material (3-32 mass %) rich in linoleic acid (up to 515 mg/g) and total phenolic compounds (up to 30 mg GAE/g).",
publisher = "Elsevier Ltd",
journal = "Journal of CO2 Utilization",
title = "Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process",
pages = "102134",
volume = "63",
doi = "10.1016/j.jcou.2022.102134"
}

Milovanović, S., Lukić, I., Kaminski, P., Debczak, A., Klimkowska, K., Tyskiewicz, K.,& Konkol, M.. (2022). Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process. in Journal of CO2 Utilization
Elsevier Ltd., 63, 102134.
https://doi.org/10.1016/j.jcou.2022.102134

Milovanović S, Lukić I, Kaminski P, Debczak A, Klimkowska K, Tyskiewicz K, Konkol M. Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process. in Journal of CO2 Utilization. 2022;63:102134.
doi:10.1016/j.jcou.2022.102134 .

Milovanović, Stoja, Lukić, Ivana, Kaminski, Piotr, Debczak, Agnieszka, Klimkowska, Kamila, Tyskiewicz, Katarzyna, Konkol, Marcin, "Green manufacturing of high-value extracts from milk thistle seeds: Parameters that affect the supercritical CO2extraction process" in Journal of CO2 Utilization, 63 (2022):102134,
https://doi.org/10.1016/j.jcou.2022.102134 . .

TY  - JOUR
AU  - Milovanovic, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5163
AB  - The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique
SP  - 100796
VL  - 29
DO  - 10.1016/j.scp.2022.100796
ER  - 

@article{
author = "Milovanovic, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique",
pages = "100796",
volume = "29",
doi = "10.1016/j.scp.2022.100796"
}

Milovanovic, S., Grzegorczyk, A., Świątek, Ł., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2022). Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 29, 100796.
https://doi.org/10.1016/j.scp.2022.100796

Milovanovic S, Grzegorczyk A, Świątek Ł, Dębczak A, Tyskiewicz K, Konkol M. Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy. 2022;29:100796.
doi:10.1016/j.scp.2022.100796 .

Milovanovic, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique" in Sustainable Chemistry and Pharmacy, 29 (2022):100796,
https://doi.org/10.1016/j.scp.2022.100796 . .