TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bukara, Katarina
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica
AU  - Veljović, Đorđe
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3329
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
EP  - 1055
IS  - 4
SP  - 1046
VL  - 32
DO  - 10.1002/btpr.2304
ER  - 

@article{
author = "Drvenica, Ivana and Bukara, Katarina and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš B. and Đorđević, Verica and Veljović, Đorđe and Belić, Aleksandar and Bugarski, Branko",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
pages = "1055-1046",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304"
}

Drvenica, I., Bukara, K., Ilić, V. Lj., Mišić, D., Vasić, B., Gajić, R. B., Đorđević, V., Veljović, Đ., Belić, A.,& Bugarski, B.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress
Wiley, Hoboken., 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica I, Bukara K, Ilić VL, Mišić D, Vasić B, Gajić RB, Đorđević V, Veljović Đ, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana, Bukara, Katarina, Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav, Gajić, Radoš B., Đorđević, Verica, Veljović, Đorđe, Belić, Aleksandar, Bugarski, Branko, "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna Lj.
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3318
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
EP  - 22
SP  - 14
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna Lj. and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
pages = "22-14",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017"
}

Bukara, K., Drvenica, I., Ilić, V. Lj., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić VL, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna Lj., Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Đorđević, Verica
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Veljović, Đorđe
AU  - Misić, Danijela M.
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2738
AB  - The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
EP  - 259
SP  - 250
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Đorđević, Verica and Bukara, Katarina and Mojsilović, Slavko B. and Nedović, Viktor and Bugarski, Diana and Veljović, Đorđe and Misić, Danijela M. and Bugarski, Branko",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization",
pages = "259-250",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043"
}

Kostić, I. T., Ilić, V. Lj., Đorđević, V., Bukara, K., Mojsilović, S. B., Nedović, V., Bugarski, D., Veljović, Đ., Misić, D. M.,& Bugarski, B.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostić IT, Ilić VL, Đorđević V, Bukara K, Mojsilović SB, Nedović V, Bugarski D, Veljović Đ, Misić DM, Bugarski B. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostić, Ivana T., Ilić, Vesna Lj., Đorđević, Verica, Bukara, Katarina, Mojsilović, Slavko B., Nedović, Viktor, Bugarski, Diana, Veljović, Đorđe, Misić, Danijela M., Bugarski, Branko, "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization" in Colloids and Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .