TY  - CONF
AU  - Milovanović, Stoja
AU  - Lukić, Ivana
AU  - Tadić, Vanja
AU  - Frank, Almut
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6893
AB  - Supercritical CO2 impregnation (SCI) process allows incorporation of bioactive components into solid carriers in an environmentally friendly manner. In recent years, SCI was highlighted as a promising method for preparation of added value materials that can protect bioactive components from environment (sun light, air, moisture etc.), stabilise them, and increase their shelf life. SCI process is also suitable for preparation of material with controlled release of bioactive component. Taking into account the above-mentioned, SCI process was employed in this study for incorporation of hemp seed oil (HSO) into biocompatible polymers (starch xerogel, starch aerogel, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin). Given that efficiency of SCI depends on process conditions and affinity of bioactive component towards scCO2 and polymer, it is necessary to optimise process for every individual system. Proposed SCI process was performed at pressure of 30 MPa and temperature of 40 °C during 5 h and 18 h. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after process. Additionally, FTIR and UV-Vis analysis showed that HSO is present in all tested polymers after SCI process. It was shown that impregnation of HSO is highly dependent on selection of polymeric carrier (its chemical composition and morphology) and operating time employed, resulting in loadings from 0.5% to 40%. Chemical analysis showed that HSO is rich in unsaturated fatty acids especially linoleic acid (54-60%) and α-linolenic acid (17-20%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases. Proposed SCI process gave guidelines for development of added-value materials that are solvent free and can be used as phytopharmaceuticals.
C3  - Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021
T1  - Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6893
ER  - 

@conference{
author = "Milovanović, Stoja and Lukić, Ivana and Tadić, Vanja and Frank, Almut",
year = "2021",
abstract = "Supercritical CO2 impregnation (SCI) process allows incorporation of bioactive components into solid carriers in an environmentally friendly manner. In recent years, SCI was highlighted as a promising method for preparation of added value materials that can protect bioactive components from environment (sun light, air, moisture etc.), stabilise them, and increase their shelf life. SCI process is also suitable for preparation of material with controlled release of bioactive component. Taking into account the above-mentioned, SCI process was employed in this study for incorporation of hemp seed oil (HSO) into biocompatible polymers (starch xerogel, starch aerogel, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin). Given that efficiency of SCI depends on process conditions and affinity of bioactive component towards scCO2 and polymer, it is necessary to optimise process for every individual system. Proposed SCI process was performed at pressure of 30 MPa and temperature of 40 °C during 5 h and 18 h. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after process. Additionally, FTIR and UV-Vis analysis showed that HSO is present in all tested polymers after SCI process. It was shown that impregnation of HSO is highly dependent on selection of polymeric carrier (its chemical composition and morphology) and operating time employed, resulting in loadings from 0.5% to 40%. Chemical analysis showed that HSO is rich in unsaturated fatty acids especially linoleic acid (54-60%) and α-linolenic acid (17-20%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases. Proposed SCI process gave guidelines for development of added-value materials that are solvent free and can be used as phytopharmaceuticals.",
journal = "Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021",
title = "Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6893"
}

Milovanović, S., Lukić, I., Tadić, V.,& Frank, A.. (2021). Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil. in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021, 47.
https://hdl.handle.net/21.15107/rcub_technorep_6893

Milovanović S, Lukić I, Tadić V, Frank A. Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil. in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021. 2021;:47.
https://hdl.handle.net/21.15107/rcub_technorep_6893 .

Milovanović, Stoja, Lukić, Ivana, Tadić, Vanja, Frank, Almut, "Supercritical CO2 impregnation - selection of suitable polymeric carrier for hemp seed oil" in Book of Abstracts / 1st GREENERING International Conference, COST Action GREENERING CA 18224 Green Chemical Engineering Network towards upscaling sustainable processes, 15th - 16th February 2021 (2021):47,
https://hdl.handle.net/21.15107/rcub_technorep_6893 .

TY  - JOUR
AU  - Tadic, Vanja M.
AU  - Zugic, Ana
AU  - Martinovic, Milica
AU  - Stankovic, Milica
AU  - Maksimović, Svetolik
AU  - Frank, Almut
AU  - Nesic, Ivana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4821
AB  - Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.
T2  - Pharmaceutics
T1  - Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)
IS  - 11
VL  - 13
DO  - 10.3390/pharmaceutics13111919
ER  - 

@article{
author = "Tadic, Vanja M. and Zugic, Ana and Martinovic, Milica and Stankovic, Milica and Maksimović, Svetolik and Frank, Almut and Nesic, Ivana",
year = "2021",
abstract = "Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.",
journal = "Pharmaceutics",
title = "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)",
number = "11",
volume = "13",
doi = "10.3390/pharmaceutics13111919"
}

Tadic, V. M., Zugic, A., Martinovic, M., Stankovic, M., Maksimović, S., Frank, A.,& Nesic, I.. (2021). Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics, 13(11).
https://doi.org/10.3390/pharmaceutics13111919

Tadic VM, Zugic A, Martinovic M, Stankovic M, Maksimović S, Frank A, Nesic I. Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics. 2021;13(11).
doi:10.3390/pharmaceutics13111919 .

Tadic, Vanja M., Zugic, Ana, Martinovic, Milica, Stankovic, Milica, Maksimović, Svetolik, Frank, Almut, Nesic, Ivana, "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)" in Pharmaceutics, 13, no. 11 (2021),
https://doi.org/10.3390/pharmaceutics13111919 . .