TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Stanković, Milica
AU  - Roganović, Sonja
AU  - Nešić, Ivana
AU  - Zvezdanović, Jelena
AU  - Tadić, Vanja
AU  - Žižović, Irena
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5230
AB  - Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.
PB  - Elsevier Ireland Ltd
T2  - Journal of Ethnopharmacology
T1  - Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin
SP  - 115779
VL  - 301
DO  - 10.1016/j.jep.2022.115779
ER  - 

@article{
author = "Maksimović, Svetolik and Stanković, Milica and Roganović, Sonja and Nešić, Ivana and Zvezdanović, Jelena and Tadić, Vanja and Žižović, Irena",
year = "2023",
abstract = "Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.",
publisher = "Elsevier Ireland Ltd",
journal = "Journal of Ethnopharmacology",
title = "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin",
pages = "115779",
volume = "301",
doi = "10.1016/j.jep.2022.115779"
}

Maksimović, S., Stanković, M., Roganović, S., Nešić, I., Zvezdanović, J., Tadić, V.,& Žižović, I.. (2023). Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology
Elsevier Ireland Ltd., 301, 115779.
https://doi.org/10.1016/j.jep.2022.115779

Maksimović S, Stanković M, Roganović S, Nešić I, Zvezdanović J, Tadić V, Žižović I. Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology. 2023;301:115779.
doi:10.1016/j.jep.2022.115779 .

Maksimović, Svetolik, Stanković, Milica, Roganović, Sonja, Nešić, Ivana, Zvezdanović, Jelena, Tadić, Vanja, Žižović, Irena, "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin" in Journal of Ethnopharmacology, 301 (2023):115779,
https://doi.org/10.1016/j.jep.2022.115779 . .

TY  - BOOK
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Nešić, Ivana
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Milovanović, Stoja
AU  - Žugić, Ana
AU  - Aksentijević, Ksenija
AU  - Stanković, Milica
AU  - Ivanović, Jasna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6876
PB  - Niš : Galaksijanis
T1  - Smilje od tradicionalne do naučno zasnovane primene
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6876
ER  - 

@book{
author = "Tadić, Vanja and Maksimović, Svetolik and Nešić, Ivana and Žižović, Irena and Mišić, Dušan and Milovanović, Stoja and Žugić, Ana and Aksentijević, Ksenija and Stanković, Milica and Ivanović, Jasna",
year = "2021",
publisher = "Niš : Galaksijanis",
title = "Smilje od tradicionalne do naučno zasnovane primene",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6876"
}

Tadić, V., Maksimović, S., Nešić, I., Žižović, I., Mišić, D., Milovanović, S., Žugić, A., Aksentijević, K., Stanković, M.,& Ivanović, J.. (2021). Smilje od tradicionalne do naučno zasnovane primene. 
Niš : Galaksijanis..
https://hdl.handle.net/21.15107/rcub_technorep_6876

Tadić V, Maksimović S, Nešić I, Žižović I, Mišić D, Milovanović S, Žugić A, Aksentijević K, Stanković M, Ivanović J. Smilje od tradicionalne do naučno zasnovane primene. 2021;.
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

Tadić, Vanja, Maksimović, Svetolik, Nešić, Ivana, Žižović, Irena, Mišić, Dušan, Milovanović, Stoja, Žugić, Ana, Aksentijević, Ksenija, Stanković, Milica, Ivanović, Jasna, "Smilje od tradicionalne do naučno zasnovane primene" (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6876 .

TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
AU  - Ivanović, Jasna
AU  - Radmanović, Tanja
AU  - Milovanović, Stoja
AU  - Stanković, Milica
AU  - Žižović, Irena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3867
AB  - Supercritical CO2 extraction of Helichrysum italicum and impregnation of starch xerogels with the extract by using an integrated scCO(2) extraction and impregnation process were performed at 350 bar and 40 degrees C in order to produce biomaterials for possible oral intake of the extract. Xerogels produced by air-drying of acetogels and alcogels were used as carriers in the supercritical impregnation process. The effect of ethanol as a co-solvent, contact time, plant material/carrier mass ratio and xerogel preparation on the impregnation loading was studied. The highest impregnation loading (1.261 +/- 0.22%) was achieved after 5 h impregnation of the xerogel obtained from alcogel using pure scCO(2) and plant material/carrier mass ratio of 10. Chemical analysis of the extracts showed that the addition of ethanol as co-solvent had a positive effect on scCO(2) selectivity to terpene fraction and total flavonoids, while it lowered the total phenolic content. Despite the difference in chemical composition, both extracts expressed similar antioxidant activity according to the DPPH and FRAP methods. The integrated process was shown to be a feasible method for isolation and incorporation of bioactive components of H italicum into starch xerogels.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel
EP  - 200
IS  - 2
SP  - 191
VL  - 24
DO  - 10.2298/CICEQ170223031M
ER  - 

@article{
author = "Maksimović, Svetolik and Tadić, Vanja and Ivanović, Jasna and Radmanović, Tanja and Milovanović, Stoja and Stanković, Milica and Žižović, Irena",
year = "2018",
abstract = "Supercritical CO2 extraction of Helichrysum italicum and impregnation of starch xerogels with the extract by using an integrated scCO(2) extraction and impregnation process were performed at 350 bar and 40 degrees C in order to produce biomaterials for possible oral intake of the extract. Xerogels produced by air-drying of acetogels and alcogels were used as carriers in the supercritical impregnation process. The effect of ethanol as a co-solvent, contact time, plant material/carrier mass ratio and xerogel preparation on the impregnation loading was studied. The highest impregnation loading (1.261 +/- 0.22%) was achieved after 5 h impregnation of the xerogel obtained from alcogel using pure scCO(2) and plant material/carrier mass ratio of 10. Chemical analysis of the extracts showed that the addition of ethanol as co-solvent had a positive effect on scCO(2) selectivity to terpene fraction and total flavonoids, while it lowered the total phenolic content. Despite the difference in chemical composition, both extracts expressed similar antioxidant activity according to the DPPH and FRAP methods. The integrated process was shown to be a feasible method for isolation and incorporation of bioactive components of H italicum into starch xerogels.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel",
pages = "200-191",
number = "2",
volume = "24",
doi = "10.2298/CICEQ170223031M"
}

Maksimović, S., Tadić, V., Ivanović, J., Radmanović, T., Milovanović, S., Stanković, M.,& Žižović, I.. (2018). Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 24(2), 191-200.
https://doi.org/10.2298/CICEQ170223031M

Maksimović S, Tadić V, Ivanović J, Radmanović T, Milovanović S, Stanković M, Žižović I. Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel. in Chemical Industry & Chemical Engineering Quarterly. 2018;24(2):191-200.
doi:10.2298/CICEQ170223031M .

Maksimović, Svetolik, Tadić, Vanja, Ivanović, Jasna, Radmanović, Tanja, Milovanović, Stoja, Stanković, Milica, Žižović, Irena, "Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel" in Chemical Industry & Chemical Engineering Quarterly, 24, no. 2 (2018):191-200,
https://doi.org/10.2298/CICEQ170223031M . .

TY  - JOUR
AU  - Stanković, Milica
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3811
AB  - Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry.
AB  - Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique
T1  - Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije
EP  - 200
IS  - 3
SP  - 193
VL  - 35
DO  - 10.2478/afmnai-2018-0021
ER  - 

@article{
author = "Stanković, Milica and Maksimović, Svetolik and Tadić, Vanja and Arsić, Ivana A.",
year = "2018",
abstract = "Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry., Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique, Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije",
pages = "200-193",
number = "3",
volume = "35",
doi = "10.2478/afmnai-2018-0021"
}

Stanković, M., Maksimović, S., Tadić, V.,& Arsić, I. A.. (2018). The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 35(3), 193-200.
https://doi.org/10.2478/afmnai-2018-0021

Stanković M, Maksimović S, Tadić V, Arsić IA. The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis. 2018;35(3):193-200.
doi:10.2478/afmnai-2018-0021 .

Stanković, Milica, Maksimović, Svetolik, Tadić, Vanja, Arsić, Ivana A., "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique" in Acta Facultatis Medicae Naissensis, 35, no. 3 (2018):193-200,
https://doi.org/10.2478/afmnai-2018-0021 . .