TY  - JOUR
AU  - Vukmirović, Saša
AU  - Ilić, Vladimirka
AU  - Tadić, Vanja
AU  - Čapo, Ivan
AU  - Pavlović, Nebojša
AU  - Tomas, Ana
AU  - Paut Kusturica, Milica
AU  - Tomić, Nataša
AU  - Maksimović, Svetolik
AU  - Stilinović, Nebojša
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6221
AB  - The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.
PB  - MDPI
T2  - Antioxidants
T1  - Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.
IS  - 2
SP  - 382
VL  - 12
DO  - 10.3390/antiox12020382
ER  - 

@article{
author = "Vukmirović, Saša and Ilić, Vladimirka and Tadić, Vanja and Čapo, Ivan and Pavlović, Nebojša and Tomas, Ana and Paut Kusturica, Milica and Tomić, Nataša and Maksimović, Svetolik and Stilinović, Nebojša",
year = "2023",
abstract = "The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.",
number = "2",
pages = "382",
volume = "12",
doi = "10.3390/antiox12020382"
}

Vukmirović, S., Ilić, V., Tadić, V., Čapo, I., Pavlović, N., Tomas, A., Paut Kusturica, M., Tomić, N., Maksimović, S.,& Stilinović, N.. (2023). Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants
MDPI., 12(2), 382.
https://doi.org/10.3390/antiox12020382

Vukmirović S, Ilić V, Tadić V, Čapo I, Pavlović N, Tomas A, Paut Kusturica M, Tomić N, Maksimović S, Stilinović N. Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants. 2023;12(2):382.
doi:10.3390/antiox12020382 .

Vukmirović, Saša, Ilić, Vladimirka, Tadić, Vanja, Čapo, Ivan, Pavlović, Nebojša, Tomas, Ana, Paut Kusturica, Milica, Tomić, Nataša, Maksimović, Svetolik, Stilinović, Nebojša, "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L." in Antioxidants, 12, no. 2 (2023):382,
https://doi.org/10.3390/antiox12020382 . .