TY  - JOUR
AU  - Prlainović, Nevena
AU  - Bezbradica, Dejan
AU  - Knežević-Jugović, Zorica
AU  - Kozlowska, Roksana T.
AU  - Mijin, Dušan
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1563
AB  - The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.
PB  - Sociedade Brasileira de Química
T2  - Journal of the Brazilian Chemical Society
T1  - A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone
EP  - 2293
IS  - 12
SP  - 2285
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1563
ER  - 

@article{
author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Kozlowska, Roksana T. and Mijin, Dušan",
year = "2010",
abstract = "The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.",
publisher = "Sociedade Brasileira de Química",
journal = "Journal of the Brazilian Chemical Society",
title = "A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone",
pages = "2293-2285",
number = "12",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1563"
}

Prlainović, N., Bezbradica, D., Knežević-Jugović, Z., Kozlowska, R. T.,& Mijin, D.. (2010). A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone. in Journal of the Brazilian Chemical Society
Sociedade Brasileira de Química., 21(12), 2285-2293.
https://hdl.handle.net/21.15107/rcub_technorep_1563

Prlainović N, Bezbradica D, Knežević-Jugović Z, Kozlowska RT, Mijin D. A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone. in Journal of the Brazilian Chemical Society. 2010;21(12):2285-2293.
https://hdl.handle.net/21.15107/rcub_technorep_1563 .

Prlainović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, Kozlowska, Roksana T., Mijin, Dušan, "A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone" in Journal of the Brazilian Chemical Society, 21, no. 12 (2010):2285-2293,
https://hdl.handle.net/21.15107/rcub_technorep_1563 .