TY  - JOUR
AU  - Pecarski, Danijela
AU  - Knežević-Jugović, Zorica
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajilovski, Katarina
AU  - Janković, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2616
AB  - The aim of this study was to evaluate and compare the antimicrobial activity of four essential oils that belong to Lamiaceae family (sage, oregano, thyme) and eucalyptus oil. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that the highest percent of essential oils 98.93% include three classes of compounds - monoterpene hydrocarbons, aromatic hydrocarbons and oxidized monoterpenes. The main components are oxidized monoterpenes: carvacrol (59.03%); thymol (36.12%), eucalyptol (20.66%), hydrocarbon monoterpenes: limonene (30.96%) and a-pinene (12.21%) and aromatic monoterpene, p-cymene (22.25%) All essential oils showed great potential of antimicrobial activity against several bacteria and yeast C. albicans, using the agar diffusion method with wells. Minimum inhibitory concentration (MIC) for the essential oils has been determined by the broth dilution method and valued in the range from 1 to 5 μL/m, depending on the essential oil and bacteria tested, and up to 100 μL/ml for C. albicans. The essential oils of Lamiaceae family exhibited a strong antibacterial activity for tested microorganisms, while the essential oils of thyme were especially recognized.
AB  - Ova studija se bavi ispitivanjem i upoređivanjem antimikrobnog dejstva etarskih ulja familije Lamiaceae: timijana, žalfije, origana i etarskog ulja eukaliptusa. Gasnom htomatografijom sa masenom spektrometrijom (GC-MS) je određena hemijska struktura i zastupljenost najvažnijih komponenata, pri čemu 98,92% sadržaja etarskog ulja zauzimaju tri klase jedinjenja, ugljovodonični monoterpeni, aromatični ugljovodonici i oksidovani monoterpeni, dok ostalim grupama jedinjena pripada manje od 1% sadržaja. Glavne komponenete ispitanih etarskih ulja su oksidovani monoterpeni: karvakrol (59,03%), timol (36,12%), eukaliptol (20,66%), i ugljovodonični monoterpeni: limonen (30,96%), a-pinen (12,21%), kao i aromatični monoterpen p-cimen (22,25%). Sva etarska ulja su pokazala značajnu anitimikrobnu aktivnst protiv svih testiranih bakterija i C.albicans, metodom difuzije u bunarčićima na agarnoj podlozi. Minimalna inhibitorna koncentracija je određena dilucionom metodom, i utvrđeno je da se vrednosti za MIC kreću u opsegu od 1μL/ml - 5 μL/ml za testirane bakterije, zavisno od ispitivanog etarskog ulja i bakterije koja se testira, i do 100 μL/ml za C. albicans. Etarska ulja familije Lamiaceae pokazuju snažno antimikrobno dejstvo, među kojima se posebno izdvaja etarsko ulje timijana.
PB  - Faculty of Technology, Novi Sad
T2  - Acta periodica technologica
T1  - Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M)
T1  - Komparativna analiza hemijskog sastava i antimikrobne aktivnosti nekih etarskih ulja familije Lamiaceae i etarskog ulja eukaliptusa (Eucaliptus globules M)
EP  - 213
IS  - 45
SP  - 201
DO  - 10.2298/APT1445201P
ER  - 

@article{
author = "Pecarski, Danijela and Knežević-Jugović, Zorica and Dimitrijević-Branković, Suzana and Mihajilovski, Katarina and Janković, Slobodan",
year = "2014",
abstract = "The aim of this study was to evaluate and compare the antimicrobial activity of four essential oils that belong to Lamiaceae family (sage, oregano, thyme) and eucalyptus oil. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that the highest percent of essential oils 98.93% include three classes of compounds - monoterpene hydrocarbons, aromatic hydrocarbons and oxidized monoterpenes. The main components are oxidized monoterpenes: carvacrol (59.03%); thymol (36.12%), eucalyptol (20.66%), hydrocarbon monoterpenes: limonene (30.96%) and a-pinene (12.21%) and aromatic monoterpene, p-cymene (22.25%) All essential oils showed great potential of antimicrobial activity against several bacteria and yeast C. albicans, using the agar diffusion method with wells. Minimum inhibitory concentration (MIC) for the essential oils has been determined by the broth dilution method and valued in the range from 1 to 5 μL/m, depending on the essential oil and bacteria tested, and up to 100 μL/ml for C. albicans. The essential oils of Lamiaceae family exhibited a strong antibacterial activity for tested microorganisms, while the essential oils of thyme were especially recognized., Ova studija se bavi ispitivanjem i upoređivanjem antimikrobnog dejstva etarskih ulja familije Lamiaceae: timijana, žalfije, origana i etarskog ulja eukaliptusa. Gasnom htomatografijom sa masenom spektrometrijom (GC-MS) je određena hemijska struktura i zastupljenost najvažnijih komponenata, pri čemu 98,92% sadržaja etarskog ulja zauzimaju tri klase jedinjenja, ugljovodonični monoterpeni, aromatični ugljovodonici i oksidovani monoterpeni, dok ostalim grupama jedinjena pripada manje od 1% sadržaja. Glavne komponenete ispitanih etarskih ulja su oksidovani monoterpeni: karvakrol (59,03%), timol (36,12%), eukaliptol (20,66%), i ugljovodonični monoterpeni: limonen (30,96%), a-pinen (12,21%), kao i aromatični monoterpen p-cimen (22,25%). Sva etarska ulja su pokazala značajnu anitimikrobnu aktivnst protiv svih testiranih bakterija i C.albicans, metodom difuzije u bunarčićima na agarnoj podlozi. Minimalna inhibitorna koncentracija je određena dilucionom metodom, i utvrđeno je da se vrednosti za MIC kreću u opsegu od 1μL/ml - 5 μL/ml za testirane bakterije, zavisno od ispitivanog etarskog ulja i bakterije koja se testira, i do 100 μL/ml za C. albicans. Etarska ulja familije Lamiaceae pokazuju snažno antimikrobno dejstvo, među kojima se posebno izdvaja etarsko ulje timijana.",
publisher = "Faculty of Technology, Novi Sad",
journal = "Acta periodica technologica",
title = "Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M), Komparativna analiza hemijskog sastava i antimikrobne aktivnosti nekih etarskih ulja familije Lamiaceae i etarskog ulja eukaliptusa (Eucaliptus globules M)",
pages = "213-201",
number = "45",
doi = "10.2298/APT1445201P"
}

Pecarski, D., Knežević-Jugović, Z., Dimitrijević-Branković, S., Mihajilovski, K.,& Janković, S.. (2014). Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M). in Acta periodica technologica
Faculty of Technology, Novi Sad.(45), 201-213.
https://doi.org/10.2298/APT1445201P

Pecarski D, Knežević-Jugović Z, Dimitrijević-Branković S, Mihajilovski K, Janković S. Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M). in Acta periodica technologica. 2014;(45):201-213.
doi:10.2298/APT1445201P .

Pecarski, Danijela, Knežević-Jugović, Zorica, Dimitrijević-Branković, Suzana, Mihajilovski, Katarina, Janković, Slobodan, "Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and eucaliptus (Eucaliptus globules M)" in Acta periodica technologica, no. 45 (2014):201-213,
https://doi.org/10.2298/APT1445201P . .

TY  - JOUR
AU  - Pecarski, Danijela
AU  - Knežević-Jugović, Zorica
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajilovski, Katarina
AU  - Janković, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2643
AB  - Development of an available drug delivery system is of great interest considering the therapeutic effects of formulations with violate essential oils, especially assuming the fact that using essential oils as antimicrobial agents is a rather expanded in antimicrobial therapy nowadays. In this paper, the chitosan microparticles with encapsulated thyme essential oil were prepared in the emulsion by cross-linking method. The effect of thyme oil and glutaraldehyde initial concentrations on particle size, morphology, and particle size distribution was investigated. In addition, the influence of these parameters on the encapsulation of thyme oil in chitosan microparticles, concerning thyme oil loadings and encapsulation efficiency was also tested. The particles showed a spherical shape with an average diameter of 4.71±1.42 to 13.65±4.34 μm, depending on the concentration of the essential oil and glutaraldehyde that were used. The diameter of microparticles appeared to increase with increasing the thyme essential oil concentration, and decreased with the increase of glutaraldehyde concentration. It was shown that the concentration of glutaraldehyde did not affect the degree of encapsulation, but the increase in the initial concentration of thyme oil increased the degree of encapsulation of this essential oil in chitosan microparticles. All particles containing thyme essential oil, as well as essential oil of thyme itself showed significant antimicrobial activity against Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 and Enterococcus faecalis ATCC 25929. This study showed a great potential of the use of thyme essential oil as an antimicrobial agent, especially when encapsulated in a drug delivery system with controlled release of the active antimicrobial component.
AB  - Etarska ulja kao antimikrobni agensi su sve više u upoterbi u antimikrobnoj terapiji, pa je i razvoj odgovarajućeg drug delivery sistema sa etarskim uljem od velikog terapijskog značaja. Inkapsulacija isparljivih etrskih ulja u odgovarajuće čestice je od velikog značaja da bi se žaštitile bioaktivne komponente, koje su sastavni deo etarskih ulja i koje su osetljive na direktno delovanje svetlosti, temperature i oksidacije jer ovi faktori menjaju njihove biohemijske osobine. Hitozan je biodegradibilan, biokompatibilan, mukoadhezivan i netoksičan prirodni polimer koji kao takav ima ogroman potencijal kao nosač aktivnih supstanci u farmaceutskim formulacijama. Hitozanske mikročestice sa inkapsuliranim etarskim uljem timijana su izrađene emulzionom metodom uz primenu urežavajućeg agensa. Varirane su koncentracije etarskog ulja timijana i glutaraldehida kao umreživača, i praćen je njhov uticaj na veličinu čestica, količinu inkapsuliranog timijana i efikasnost inkapsulacije. Hitozanske mikročestice su imale sferičan oblik sa prosečnim prečnikom koji je bio u opsegu od 4.71±1.42 to 13.65±4.34 μm, u zavisnosti od koncentracije etarskog ulja i glutaraldehida koji su korišćeni pri izradi čestica. Prečnik čestica, odnosno veličina čestica, je rasla sa povećanjem koncentracije etarskog ulja timijana i smanjivala se sa povećanjem koncentracije glutaraldehida. Pokazano je da koncentracija glutaraldehida nema uticaja na stepen inkapsulacije etarskog ulja timijana u hitozanske mikročestice, dok sa porastom koncentracije etarskog ulja timijana raste količina inkapsuliranog ulja i efikasnost inkapsulacije. Sve mikročestice, uključujući i samo etarsko ulje timijana, su pokazali značajnu antimikrobnu aktivnost protiv Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 i Enterococcus faecalis ATCC 25929. Ova studija je pokazala veliki antimikrobni potencijal etarskog ulja timijana inkorporiranog u hitozanske mikročestice kao odgovarajuću farmaceutsku formulaciju sa kontrolisanim oslobađanjem aktivne antimikrobne komponente.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil
T1  - Izrada, karakterizacija i antimikrobna aktivnost hitozanskih mikročestica sa etarskim uljem timijana
EP  - 729
IS  - 6
SP  - 721
VL  - 68
DO  - 10.2298/HEMIND140415048P
ER  - 

@article{
author = "Pecarski, Danijela and Knežević-Jugović, Zorica and Dimitrijević-Branković, Suzana and Mihajilovski, Katarina and Janković, Slobodan",
year = "2014",
abstract = "Development of an available drug delivery system is of great interest considering the therapeutic effects of formulations with violate essential oils, especially assuming the fact that using essential oils as antimicrobial agents is a rather expanded in antimicrobial therapy nowadays. In this paper, the chitosan microparticles with encapsulated thyme essential oil were prepared in the emulsion by cross-linking method. The effect of thyme oil and glutaraldehyde initial concentrations on particle size, morphology, and particle size distribution was investigated. In addition, the influence of these parameters on the encapsulation of thyme oil in chitosan microparticles, concerning thyme oil loadings and encapsulation efficiency was also tested. The particles showed a spherical shape with an average diameter of 4.71±1.42 to 13.65±4.34 μm, depending on the concentration of the essential oil and glutaraldehyde that were used. The diameter of microparticles appeared to increase with increasing the thyme essential oil concentration, and decreased with the increase of glutaraldehyde concentration. It was shown that the concentration of glutaraldehyde did not affect the degree of encapsulation, but the increase in the initial concentration of thyme oil increased the degree of encapsulation of this essential oil in chitosan microparticles. All particles containing thyme essential oil, as well as essential oil of thyme itself showed significant antimicrobial activity against Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 and Enterococcus faecalis ATCC 25929. This study showed a great potential of the use of thyme essential oil as an antimicrobial agent, especially when encapsulated in a drug delivery system with controlled release of the active antimicrobial component., Etarska ulja kao antimikrobni agensi su sve više u upoterbi u antimikrobnoj terapiji, pa je i razvoj odgovarajućeg drug delivery sistema sa etarskim uljem od velikog terapijskog značaja. Inkapsulacija isparljivih etrskih ulja u odgovarajuće čestice je od velikog značaja da bi se žaštitile bioaktivne komponente, koje su sastavni deo etarskih ulja i koje su osetljive na direktno delovanje svetlosti, temperature i oksidacije jer ovi faktori menjaju njihove biohemijske osobine. Hitozan je biodegradibilan, biokompatibilan, mukoadhezivan i netoksičan prirodni polimer koji kao takav ima ogroman potencijal kao nosač aktivnih supstanci u farmaceutskim formulacijama. Hitozanske mikročestice sa inkapsuliranim etarskim uljem timijana su izrađene emulzionom metodom uz primenu urežavajućeg agensa. Varirane su koncentracije etarskog ulja timijana i glutaraldehida kao umreživača, i praćen je njhov uticaj na veličinu čestica, količinu inkapsuliranog timijana i efikasnost inkapsulacije. Hitozanske mikročestice su imale sferičan oblik sa prosečnim prečnikom koji je bio u opsegu od 4.71±1.42 to 13.65±4.34 μm, u zavisnosti od koncentracije etarskog ulja i glutaraldehida koji su korišćeni pri izradi čestica. Prečnik čestica, odnosno veličina čestica, je rasla sa povećanjem koncentracije etarskog ulja timijana i smanjivala se sa povećanjem koncentracije glutaraldehida. Pokazano je da koncentracija glutaraldehida nema uticaja na stepen inkapsulacije etarskog ulja timijana u hitozanske mikročestice, dok sa porastom koncentracije etarskog ulja timijana raste količina inkapsuliranog ulja i efikasnost inkapsulacije. Sve mikročestice, uključujući i samo etarsko ulje timijana, su pokazali značajnu antimikrobnu aktivnost protiv Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Candida albicans ATCC 24433 i Enterococcus faecalis ATCC 25929. Ova studija je pokazala veliki antimikrobni potencijal etarskog ulja timijana inkorporiranog u hitozanske mikročestice kao odgovarajuću farmaceutsku formulaciju sa kontrolisanim oslobađanjem aktivne antimikrobne komponente.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil, Izrada, karakterizacija i antimikrobna aktivnost hitozanskih mikročestica sa etarskim uljem timijana",
pages = "729-721",
number = "6",
volume = "68",
doi = "10.2298/HEMIND140415048P"
}

Pecarski, D., Knežević-Jugović, Z., Dimitrijević-Branković, S., Mihajilovski, K.,& Janković, S.. (2014). Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(6), 721-729.
https://doi.org/10.2298/HEMIND140415048P

Pecarski D, Knežević-Jugović Z, Dimitrijević-Branković S, Mihajilovski K, Janković S. Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil. in Hemijska industrija. 2014;68(6):721-729.
doi:10.2298/HEMIND140415048P .

Pecarski, Danijela, Knežević-Jugović, Zorica, Dimitrijević-Branković, Suzana, Mihajilovski, Katarina, Janković, Slobodan, "Preparation, characterization and antimicrobial activity of chitosan microparticles with thyme essential oil" in Hemijska industrija, 68, no. 6 (2014):721-729,
https://doi.org/10.2298/HEMIND140415048P . .

TY  - JOUR
AU  - Tadić, Vanja
AU  - Bojović, Dragica
AU  - Arsić, Ivana
AU  - Đorđević, Sofija
AU  - Aksentijević, Ksenija
AU  - Stamenić, Marko
AU  - Janković, Slobodan
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2207
AB  - Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
PB  - MDPI, Basel
T2  - Molecules
T1  - Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
EP  - 2703
IS  - 3
SP  - 2683
VL  - 17
DO  - 10.3390/molecules17032683
ER  - 

@article{
author = "Tadić, Vanja and Bojović, Dragica and Arsić, Ivana and Đorđević, Sofija and Aksentijević, Ksenija and Stamenić, Marko and Janković, Slobodan",
year = "2012",
abstract = "Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts",
pages = "2703-2683",
number = "3",
volume = "17",
doi = "10.3390/molecules17032683"
}

Tadić, V., Bojović, D., Arsić, I., Đorđević, S., Aksentijević, K., Stamenić, M.,& Janković, S.. (2012). Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules
MDPI, Basel., 17(3), 2683-2703.
https://doi.org/10.3390/molecules17032683

Tadić V, Bojović D, Arsić I, Đorđević S, Aksentijević K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules. 2012;17(3):2683-2703.
doi:10.3390/molecules17032683 .

Tadić, Vanja, Bojović, Dragica, Arsić, Ivana, Đorđević, Sofija, Aksentijević, Ksenija, Stamenić, Marko, Janković, Slobodan, "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts" in Molecules, 17, no. 3 (2012):2683-2703,
https://doi.org/10.3390/molecules17032683 . .