Radotić, Ksenija

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Authority KeyName Variants
orcid::0000-0002-9770-0788
  • Radotić, Ksenija (5)
Projects
Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200023 (Institute of Technology of Nuclear and Other Mineral Row Materials - ITNMS, Belgrade)
High-Performance Computing Infrastructure for South East Europe's Research Communities Modeling and Numerical Simulations of Complex Many-Body Systems
Design, synthesis, characterization and assessment of practical applications of coordination and organometallic compounds Studies of enzyme interactions with toxic and pharmacologically active molecules
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200053 (University of Belgrade, Institute for Multidisciplinary Research)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200135 (University of Belgrade, Faculty of Technology and Metallurgy) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200287 (Innovation Center of the Faculty of Technology and Metallurgy)
Studying climate change and its influence on environment: impacts, adaptation and mitigation Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden
Ministry of Education and Science, Republic of Serbia

Author's Bibliography

Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions

Spasojević, Dragica; Prodanović, Olivera; Mutavdžić, Dragosav; Šekuljica, Nataša; Jovanović, Jelena; Maksimović, Vuk; Radotić, Ksenija

(John Wiley and Sons Inc, 2023)

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Mutavdžić, Dragosav
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6635
AB  - In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.
PB  - John Wiley and Sons Inc
T2  - Biotechnology Journal
T1  - Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions
DO  - 10.1002/biot.202300312
ER  - 
@article{
author = "Spasojević, Dragica and Prodanović, Olivera and Mutavdžić, Dragosav and Šekuljica, Nataša and Jovanović, Jelena and Maksimović, Vuk and Radotić, Ksenija",
year = "2023",
abstract = "In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.",
publisher = "John Wiley and Sons Inc",
journal = "Biotechnology Journal",
title = "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions",
doi = "10.1002/biot.202300312"
}
Spasojević, D., Prodanović, O., Mutavdžić, D., Šekuljica, N., Jovanović, J., Maksimović, V.,& Radotić, K.. (2023). Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal
John Wiley and Sons Inc..
https://doi.org/10.1002/biot.202300312
Spasojević D, Prodanović O, Mutavdžić D, Šekuljica N, Jovanović J, Maksimović V, Radotić K. Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal. 2023;.
doi:10.1002/biot.202300312 .
Spasojević, Dragica, Prodanović, Olivera, Mutavdžić, Dragosav, Šekuljica, Nataša, Jovanović, Jelena, Maksimović, Vuk, Radotić, Ksenija, "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions" in Biotechnology Journal (2023),
https://doi.org/10.1002/biot.202300312 . .

Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions

Milojkov, Dušan V.; Sokić, Miroslav D.; Živković-Radovanović, Vukosava; Manojlović, Vaso D.; Mutavdžić, Dragosav R.; Janjić, Goran V.; Radotić, Ksenija

(Springer, 2023)

TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Sokić, Miroslav D.
AU  - Živković-Radovanović, Vukosava
AU  - Manojlović, Vaso D.
AU  - Mutavdžić, Dragosav R.
AU  - Janjić, Goran V.
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5279
AB  - In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated. Uniform nanopowders were synthesized by precipitation reaction followed by centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra indicate the presence of nitrate (NO3−) and carbonate (CO32−) species. SEM analyzes confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp nanopowder occurred in the violet-blue region of the visible part of the spectrum, with redshift to the blue-green color region when Pr3+, NO3− and CO32− co-doped in the lattice. Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus aureus was investigated before and after treatment of nanopowders by UVA radiation of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and 76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials showed a low degree of hemolytic activity and could potentially be candidates for further research in dentistry.
PB  - Springer
T2  - Optical and Quantum Electronics
T1  - Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions
IS  - 1
SP  - 84
VL  - 55
DO  - 10.1007/s11082-022-04347-7
ER  - 
@article{
author = "Milojkov, Dušan V. and Sokić, Miroslav D. and Živković-Radovanović, Vukosava and Manojlović, Vaso D. and Mutavdžić, Dragosav R. and Janjić, Goran V. and Radotić, Ksenija",
year = "2023",
abstract = "In this study, luminescence and antibacterial behavior of pure fluorapatite (FAp) and praseodymium-nitrate-carbonate co-doped fluorapatite (PrNCFAp) nanopowders were investigated. Uniform nanopowders were synthesized by precipitation reaction followed by centrifugation and systematically characterized by XRD, FTIR, SEM–EDS, TG and PL methods. XRD analysis revealed the formation of hexagonal FAp crystals, and FTIR spectra indicate the presence of nitrate (NO3−) and carbonate (CO32−) species. SEM analyzes confirm agglomerates composed of irregular nanometer-sized spheres. Emission of FAp nanopowder occurred in the violet-blue region of the visible part of the spectrum, with redshift to the blue-green color region when Pr3+, NO3− and CO32− co-doped in the lattice. Analysis of luminescence spectra by MCR-ALS method extract three fluorophores from the PrNCFAp sample and showed simultaneous existents of emission-reabsorption-emission between dopants in FAp lattice. Antibacterial activity against pathogen Staphylococcus aureus was investigated before and after treatment of nanopowders by UVA radiation of 365 nm. Nanopowders irradiated with UVA compared to non-irradiated reduced Staphylococcus aureus by 84.9% for PrNCFAp and 33.3% for FAp in the first 0.5 h of contact, and 76.1% and 42.9% after 24 h of contact. In addition, the obtained luminescent nanomaterials showed a low degree of hemolytic activity and could potentially be candidates for further research in dentistry.",
publisher = "Springer",
journal = "Optical and Quantum Electronics",
title = "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions",
number = "1",
pages = "84",
volume = "55",
doi = "10.1007/s11082-022-04347-7"
}
Milojkov, D. V., Sokić, M. D., Živković-Radovanović, V., Manojlović, V. D., Mutavdžić, D. R., Janjić, G. V.,& Radotić, K.. (2023). Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics
Springer., 55(1), 84.
https://doi.org/10.1007/s11082-022-04347-7
Milojkov DV, Sokić MD, Živković-Radovanović V, Manojlović VD, Mutavdžić DR, Janjić GV, Radotić K. Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions. in Optical and Quantum Electronics. 2023;55(1):84.
doi:10.1007/s11082-022-04347-7 .
Milojkov, Dušan V., Sokić, Miroslav D., Živković-Radovanović, Vukosava, Manojlović, Vaso D., Mutavdžić, Dragosav R., Janjić, Goran V., Radotić, Ksenija, "Cascade luminescence and antibacterial behavior of fluorapatite nanopowder co-doped with Pr3+, NO3− and CO32− ions" in Optical and Quantum Electronics, 55, no. 1 (2023):84,
https://doi.org/10.1007/s11082-022-04347-7 . .
1

Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents

Milojkov, Dušan V.; Radosavljević-Mihajlović, Ana S.; Stanić, Vojislav Đ.; Nastasijević, Branislav J.; Radotić, Ksenija; Janković-Častvan, Ivona; Živković-Radovanović, Vukosava

(Elsevier B.V., 2023)

TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Radosavljević-Mihajlović, Ana S.
AU  - Stanić, Vojislav Đ.
AU  - Nastasijević, Branislav J.
AU  - Radotić, Ksenija
AU  - Janković-Častvan, Ivona
AU  - Živković-Radovanović, Vukosava
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5664
AB  - Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F− ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–spectrum antimicrobial agents for various biomedical and health care preparations.
PB  - Elsevier B.V.
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents
SP  - 112649
VL  - 239
DO  - 10.1016/j.jphotobiol.2023.112649
ER  - 
@article{
author = "Milojkov, Dušan V. and Radosavljević-Mihajlović, Ana S. and Stanić, Vojislav Đ. and Nastasijević, Branislav J. and Radotić, Ksenija and Janković-Častvan, Ivona and Živković-Radovanović, Vukosava",
year = "2023",
abstract = "Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F− ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–spectrum antimicrobial agents for various biomedical and health care preparations.",
publisher = "Elsevier B.V.",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents",
pages = "112649",
volume = "239",
doi = "10.1016/j.jphotobiol.2023.112649"
}
Milojkov, D. V., Radosavljević-Mihajlović, A. S., Stanić, V. Đ., Nastasijević, B. J., Radotić, K., Janković-Častvan, I.,& Živković-Radovanović, V.. (2023). Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry and Photobiology B: Biology
Elsevier B.V.., 239, 112649.
https://doi.org/10.1016/j.jphotobiol.2023.112649
Milojkov DV, Radosavljević-Mihajlović AS, Stanić VĐ, Nastasijević BJ, Radotić K, Janković-Častvan I, Živković-Radovanović V. Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry and Photobiology B: Biology. 2023;239:112649.
doi:10.1016/j.jphotobiol.2023.112649 .
Milojkov, Dušan V., Radosavljević-Mihajlović, Ana S., Stanić, Vojislav Đ., Nastasijević, Branislav J., Radotić, Ksenija, Janković-Častvan, Ivona, Živković-Radovanović, Vukosava, "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents" in Journal of Photochemistry and Photobiology B: Biology, 239 (2023):112649,
https://doi.org/10.1016/j.jphotobiol.2023.112649 . .
2
1

Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study

Marković, Jelena M.; Trišović, Nemanja; Mutavdžić, Dragosav; Radotić, Ksenija; Juranić, Ivan; Drakulić, Branko; Marinković, Aleksandar

(Pergamon-Elsevier Science Ltd, Oxford, 2015)

TY  - JOUR
AU  - Marković, Jelena M.
AU  - Trišović, Nemanja
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Juranić, Ivan
AU  - Drakulić, Branko
AU  - Marinković, Aleksandar
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3026
AB  - Seven symmetrical 2,6-distyrylpyridines, phenyl-substituted with hydrogen-bond donors, hydrogen-bond acceptors, halogens and hydrophobic moieties were synthesized and their spectroscopic characterization was done. Solvent effects on the absorption and fluorescence spectra were analyzed and quantified using the Kamlet-Taft and Catalan approach. The obtained results were rationalized by comparison of electrostatic potentials of the molecules in the ground and in excited state and by comparison of the frontier molecular orbitals (HOMO and LUMO), derived from quantum-mechanical calculations (HE, DFT, MP2). Analysis of the results revealed an important influence of non-specific (dispersive) interactions on the solvatochromic behavior of the compounds. 1D and 2D NMR data, in silica obtained conformational assembly of the compound, and the NMR analysis of molecular flexibility in solution (NAMFIS), were used to estimate population of conformers and to deconvolute the UV-Vis spectrum of representative derivative; inferring that the conformational assembly is more complex than was assumed in so far published literature data for this class of compounds. Along with this, the emission spectra of the representative compounds were decomposed by the Multivariate Curve Resolution analysis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study
EP  - 446
SP  - 435
VL  - 135
DO  - 10.1016/j.saa.2014.07.023
ER  - 
@article{
author = "Marković, Jelena M. and Trišović, Nemanja and Mutavdžić, Dragosav and Radotić, Ksenija and Juranić, Ivan and Drakulić, Branko and Marinković, Aleksandar",
year = "2015",
abstract = "Seven symmetrical 2,6-distyrylpyridines, phenyl-substituted with hydrogen-bond donors, hydrogen-bond acceptors, halogens and hydrophobic moieties were synthesized and their spectroscopic characterization was done. Solvent effects on the absorption and fluorescence spectra were analyzed and quantified using the Kamlet-Taft and Catalan approach. The obtained results were rationalized by comparison of electrostatic potentials of the molecules in the ground and in excited state and by comparison of the frontier molecular orbitals (HOMO and LUMO), derived from quantum-mechanical calculations (HE, DFT, MP2). Analysis of the results revealed an important influence of non-specific (dispersive) interactions on the solvatochromic behavior of the compounds. 1D and 2D NMR data, in silica obtained conformational assembly of the compound, and the NMR analysis of molecular flexibility in solution (NAMFIS), were used to estimate population of conformers and to deconvolute the UV-Vis spectrum of representative derivative; inferring that the conformational assembly is more complex than was assumed in so far published literature data for this class of compounds. Along with this, the emission spectra of the representative compounds were decomposed by the Multivariate Curve Resolution analysis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study",
pages = "446-435",
volume = "135",
doi = "10.1016/j.saa.2014.07.023"
}
Marković, J. M., Trišović, N., Mutavdžić, D., Radotić, K., Juranić, I., Drakulić, B.,& Marinković, A.. (2015). Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 135, 435-446.
https://doi.org/10.1016/j.saa.2014.07.023
Marković JM, Trišović N, Mutavdžić D, Radotić K, Juranić I, Drakulić B, Marinković A. Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2015;135:435-446.
doi:10.1016/j.saa.2014.07.023 .
Marković, Jelena M., Trišović, Nemanja, Mutavdžić, Dragosav, Radotić, Ksenija, Juranić, Ivan, Drakulić, Branko, Marinković, Aleksandar, "Solvatochromism of symmetrical 2,6-distyrylpyridines. An experimental and theoretical study" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 135 (2015):435-446,
https://doi.org/10.1016/j.saa.2014.07.023 . .
6
6
6

Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers

Prodanović, Olivera; Prokopijević, Miloš; Spasojević, Dragica; Stojanović, Željko; Radotić, Ksenija; Knežević-Jugović, Zorica; Prodanović, Radivoje

(Springer, New York, 2012)

TY  - JOUR
AU  - Prodanović, Olivera
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Knežević-Jugović, Zorica
AU  - Prodanović, Radivoje
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2187
AB  - A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.
PB  - Springer, New York
T2  - Applied Biochemistry and Biotechnology
T1  - Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers
EP  - 1301
IS  - 5
SP  - 1288
VL  - 168
DO  - 10.1007/s12010-012-9857-7
ER  - 
@article{
author = "Prodanović, Olivera and Prokopijević, Miloš and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Knežević-Jugović, Zorica and Prodanović, Radivoje",
year = "2012",
abstract = "A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers",
pages = "1301-1288",
number = "5",
volume = "168",
doi = "10.1007/s12010-012-9857-7"
}
Prodanović, O., Prokopijević, M., Spasojević, D., Stojanović, Ž., Radotić, K., Knežević-Jugović, Z.,& Prodanović, R.. (2012). Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers. in Applied Biochemistry and Biotechnology
Springer, New York., 168(5), 1288-1301.
https://doi.org/10.1007/s12010-012-9857-7
Prodanović O, Prokopijević M, Spasojević D, Stojanović Ž, Radotić K, Knežević-Jugović Z, Prodanović R. Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers. in Applied Biochemistry and Biotechnology. 2012;168(5):1288-1301.
doi:10.1007/s12010-012-9857-7 .
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