TY  - JOUR
AU  - Drvenica, Ivana
AU  - Blazevic, Ivica
AU  - Boskovic, Perica
AU  - Bratanic, Andre
AU  - Bugarski, Branko
AU  - Bilusic, Tea
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4825
AB  - Encapsulation of sinigrin in liposomes with the proliposomal method was performed in order to evaluate the effect of this process on in vitro simulated digestion and antioxidant potential of sinigrin. The recovery of sinigrin after simulated gastric and duodenal digestion of its free and liposomal forms was determined with HPLC-UV using human digestive juices. The antioxidant potential of sinigrin and sinigrin-loaded liposomes was determined with the Rancimat test as their ability to prolong oxidative stability of edible oil. The efficiency of 62% was obtained by encapsulating sinigrin in liposomes. The values of mean diameter, polydispersity index and zeta potential showed satisfactory size uniformity and physical stability of the liposomes containing sinigrin. Liposomes were shown to inhibit the digestion of sinigrin in both human gastric and intestinal juices, clearly enabling its prolonged release. Moreover, sinigrin in the liposomal form significantly prolonged the induction time of edible oil oxidation compared to its free form. The results obtained are encouraging from the point of view of a possible incorporation of the sinigrin-loaded liposomes in real functional food systems or their use as nutraceuticals.
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential
EP  - 449
IS  - 4
SP  - 441
VL  - 71
DO  - 10.31883/pjfns/143574
ER  - 

@article{
author = "Drvenica, Ivana and Blazevic, Ivica and Boskovic, Perica and Bratanic, Andre and Bugarski, Branko and Bilusic, Tea",
year = "2021",
abstract = "Encapsulation of sinigrin in liposomes with the proliposomal method was performed in order to evaluate the effect of this process on in vitro simulated digestion and antioxidant potential of sinigrin. The recovery of sinigrin after simulated gastric and duodenal digestion of its free and liposomal forms was determined with HPLC-UV using human digestive juices. The antioxidant potential of sinigrin and sinigrin-loaded liposomes was determined with the Rancimat test as their ability to prolong oxidative stability of edible oil. The efficiency of 62% was obtained by encapsulating sinigrin in liposomes. The values of mean diameter, polydispersity index and zeta potential showed satisfactory size uniformity and physical stability of the liposomes containing sinigrin. Liposomes were shown to inhibit the digestion of sinigrin in both human gastric and intestinal juices, clearly enabling its prolonged release. Moreover, sinigrin in the liposomal form significantly prolonged the induction time of edible oil oxidation compared to its free form. The results obtained are encouraging from the point of view of a possible incorporation of the sinigrin-loaded liposomes in real functional food systems or their use as nutraceuticals.",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential",
pages = "449-441",
number = "4",
volume = "71",
doi = "10.31883/pjfns/143574"
}

Drvenica, I., Blazevic, I., Boskovic, P., Bratanic, A., Bugarski, B.,& Bilusic, T.. (2021). Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential. in Polish Journal of Food and Nutrition Sciences, 71(4), 441-449.
https://doi.org/10.31883/pjfns/143574

Drvenica I, Blazevic I, Boskovic P, Bratanic A, Bugarski B, Bilusic T. Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential. in Polish Journal of Food and Nutrition Sciences. 2021;71(4):441-449.
doi:10.31883/pjfns/143574 .

Drvenica, Ivana, Blazevic, Ivica, Boskovic, Perica, Bratanic, Andre, Bugarski, Branko, Bilusic, Tea, "Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential" in Polish Journal of Food and Nutrition Sciences, 71, no. 4 (2021):441-449,
https://doi.org/10.31883/pjfns/143574 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4441
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić VL, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4483
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić VL, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - CHAP
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Bugarski, Branko
AU  - Pajić-Lijaković, Ivana
AU  - Maslovarić, I.
AU  - Ilić, Vesna Lj.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4194
AB  - For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.
T2  - Erythrocytes: Structure, Functions and Clinical Aspects
T1  - Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems
EP  - 132
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4194
ER  - 

@inbook{
author = "Drvenica, Ivana and Stančić, Ana and Bugarski, Branko and Pajić-Lijaković, Ivana and Maslovarić, I. and Ilić, Vesna Lj.",
year = "2019",
abstract = "For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.",
journal = "Erythrocytes: Structure, Functions and Clinical Aspects",
booktitle = "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems",
pages = "132-57",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4194"
}

Drvenica, I., Stančić, A., Bugarski, B., Pajić-Lijaković, I., Maslovarić, I.,& Ilić, V. Lj.. (2019). Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions and Clinical Aspects, 57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194

Drvenica I, Stančić A, Bugarski B, Pajić-Lijaković I, Maslovarić I, Ilić VL. Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions and Clinical Aspects. 2019;:57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

Drvenica, Ivana, Stančić, Ana, Bugarski, Branko, Pajić-Lijaković, Ivana, Maslovarić, I., Ilić, Vesna Lj., "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems" in Erythrocytes: Structure, Functions and Clinical Aspects (2019):57-132,
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

TY  - CONF
AU  - Stanković, Ana
AU  - Drvenica, Ivana
AU  - Đukić-Vuković, Aleksandra
AU  - Marković, Smilja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4167
AB  - Owing to a wide band gap energy (3.37 eV at RT) and a large exciton binding energy (60 meV), ZnO nanoparticles (ZnONP) have a diverse application, e.g. in electronics, optoelectronics, photocatalysis. Besides, ZnONP have a great potential in medicine for bioimaging, drug/gene delivery or as antimicrobial and anticancer agents. One of suggested governing mechanisms of the mentioned biological activities of ZnONP is based on formation of reactive oxygen species (ROS). When ZnONP absorb photon with energy equal or greater than its band gap, electrons are excited from the valence band (VB) to the conduction band (CB) leaving the holes in VB. Furthermore, the photogenerated holes (h+) and electrons (e-) migrate from bulk to surface. The photogenerated holes at the VB react with water molecules adsorbed at the particle surface to produce hydroxyl radical, while electrons in CB react with oxygen molecules generating anionic superoxide radical O2 -•. Superoxide radicals can be transformed in highly reactive OH• and so on [1]. Derivatives of this active oxygen can damage the bacterial/tumor cells [2]. However, in sufficiency ROS can damage normal cells as well. Thus, an understanding of ZnONP crystal structure-activity relationship and mechanism of ZnONP-related products formation and their consequent activity is crucial for the design of safe ZnONP based biomaterial for application in treating diseases like cancer. A series of ZnONP samples were synthesized by microwave processing of precipitate in the presence of a small amount (5 wt.%) of surfactants CTAB and citric acid. The particles crystallinity and purity were investigated by X-ray diffraction, Raman and ATR-FTIR spectroscopy. The particles morphology and texture properties were observed with field emission scanning electron microscopy (FE–SEM) and nitrogen adsorption–desorption isotherm, respectively. The optical properties were studied using UV–Vis diffuse reflectance and photoluminescence (PL) spectroscopy. ZnONP samples with different surface-to-bulk defect ratio were examined on ROS formation and antimicrobial activity. Future studies will be conducted with an aim to correlate surface-to-bulk defect ratio in ZnONP with mechanism of ROS formation and their cytotoxicity to normal and cancerous cells.
C3  - Book of Abstracts / First CA17140 COST Conference Cancer Nanomedicine – from the Bench to the Bedsid
T1  - Surfactant-Assisted Microwave Processed ZnO Nanoparticles with Optimized Surface-to-Bulk Defect Ratio For Potential Biomedical Application
EP  - 93
SP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4167
ER  - 

@conference{
author = "Stanković, Ana and Drvenica, Ivana and Đukić-Vuković, Aleksandra and Marković, Smilja",
year = "2019",
abstract = "Owing to a wide band gap energy (3.37 eV at RT) and a large exciton binding energy (60 meV), ZnO nanoparticles (ZnONP) have a diverse application, e.g. in electronics, optoelectronics, photocatalysis. Besides, ZnONP have a great potential in medicine for bioimaging, drug/gene delivery or as antimicrobial and anticancer agents. One of suggested governing mechanisms of the mentioned biological activities of ZnONP is based on formation of reactive oxygen species (ROS). When ZnONP absorb photon with energy equal or greater than its band gap, electrons are excited from the valence band (VB) to the conduction band (CB) leaving the holes in VB. Furthermore, the photogenerated holes (h+) and electrons (e-) migrate from bulk to surface. The photogenerated holes at the VB react with water molecules adsorbed at the particle surface to produce hydroxyl radical, while electrons in CB react with oxygen molecules generating anionic superoxide radical O2 -•. Superoxide radicals can be transformed in highly reactive OH• and so on [1]. Derivatives of this active oxygen can damage the bacterial/tumor cells [2]. However, in sufficiency ROS can damage normal cells as well. Thus, an understanding of ZnONP crystal structure-activity relationship and mechanism of ZnONP-related products formation and their consequent activity is crucial for the design of safe ZnONP based biomaterial for application in treating diseases like cancer. A series of ZnONP samples were synthesized by microwave processing of precipitate in the presence of a small amount (5 wt.%) of surfactants CTAB and citric acid. The particles crystallinity and purity were investigated by X-ray diffraction, Raman and ATR-FTIR spectroscopy. The particles morphology and texture properties were observed with field emission scanning electron microscopy (FE–SEM) and nitrogen adsorption–desorption isotherm, respectively. The optical properties were studied using UV–Vis diffuse reflectance and photoluminescence (PL) spectroscopy. ZnONP samples with different surface-to-bulk defect ratio were examined on ROS formation and antimicrobial activity. Future studies will be conducted with an aim to correlate surface-to-bulk defect ratio in ZnONP with mechanism of ROS formation and their cytotoxicity to normal and cancerous cells.",
journal = "Book of Abstracts / First CA17140 COST Conference Cancer Nanomedicine – from the Bench to the Bedsid",
title = "Surfactant-Assisted Microwave Processed ZnO Nanoparticles with Optimized Surface-to-Bulk Defect Ratio For Potential Biomedical Application",
pages = "93-93",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4167"
}

Stanković, A., Drvenica, I., Đukić-Vuković, A.,& Marković, S.. (2019). Surfactant-Assisted Microwave Processed ZnO Nanoparticles with Optimized Surface-to-Bulk Defect Ratio For Potential Biomedical Application. in Book of Abstracts / First CA17140 COST Conference Cancer Nanomedicine – from the Bench to the Bedsid, 93-93.
https://hdl.handle.net/21.15107/rcub_technorep_4167

Stanković A, Drvenica I, Đukić-Vuković A, Marković S. Surfactant-Assisted Microwave Processed ZnO Nanoparticles with Optimized Surface-to-Bulk Defect Ratio For Potential Biomedical Application. in Book of Abstracts / First CA17140 COST Conference Cancer Nanomedicine – from the Bench to the Bedsid. 2019;:93-93.
https://hdl.handle.net/21.15107/rcub_technorep_4167 .

Stanković, Ana, Drvenica, Ivana, Đukić-Vuković, Aleksandra, Marković, Smilja, "Surfactant-Assisted Microwave Processed ZnO Nanoparticles with Optimized Surface-to-Bulk Defect Ratio For Potential Biomedical Application" in Book of Abstracts / First CA17140 COST Conference Cancer Nanomedicine – from the Bench to the Bedsid (2019):93-93,
https://hdl.handle.net/21.15107/rcub_technorep_4167 .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Kalušević, Ana
AU  - Marković, Smilja
AU  - Dragišić-Maksimović, Jelena
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4135
AB  - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
EP  - 1117
IS  - 10
SP  - 1105
VL  - 84
DO  - 10.2298/JSC190513067D
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Kalušević, Ana and Marković, Smilja and Dragišić-Maksimović, Jelena and Nedović, Viktor and Bugarski, Branko and Ilić, Vesna Lj.",
year = "2019",
abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin",
pages = "1117-1105",
number = "10",
volume = "84",
doi = "10.2298/JSC190513067D"
}

Drvenica, I., Stančić, A., Kalušević, A., Marković, S., Dragišić-Maksimović, J., Nedović, V., Bugarski, B.,& Ilić, V. Lj.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117.
https://doi.org/10.2298/JSC190513067D

Drvenica I, Stančić A, Kalušević A, Marković S, Dragišić-Maksimović J, Nedović V, Bugarski B, Ilić VL. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society. 2019;84(10):1105-1117.
doi:10.2298/JSC190513067D .

Drvenica, Ivana, Stančić, Ana, Kalušević, Ana, Marković, Smilja, Dragišić-Maksimović, Jelena, Nedović, Viktor, Bugarski, Branko, Ilić, Vesna Lj., "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in Journal of the Serbian Chemical Society, 84, no. 10 (2019):1105-1117,
https://doi.org/10.2298/JSC190513067D . .

TY  - JOUR
AU  - Liović, Nikolina
AU  - Bošković, Perica
AU  - Drvenica, Ivana
AU  - Jambrak, Anet Rezek
AU  - Dropulić, Ana Marija
AU  - Kresić, Greta
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Zorić, Zoran
AU  - Pedisić, Sandra
AU  - Bilusić, Tea
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4249
AB  - Natural phenolic compounds are recognized as bioactive ingredients in food but can also have a role as effective alternatives to synthetic antioxidants in stability improvement of foods prone to oxidation, such as edible oils. This study aimed at the preparation and HPLC-DAD characterization of phenolic extracts from lisccinium corymbosum L. (raw, pasteurized, freeze-dried and treated with high-intensity ultrasound), and at testing their antioxidant potential in the prevention of olive oil oxidation in the native state and encapsulated into microemulsions and liposomes systems. Water-in-oil structured microemulsions used in this study were prepared using mechanical, ultrasonic, and high pressure homogenization. Liposomes with the average size of 589.1 +/- 2.9 nm were produced with the proliposome method using commercially available phosphatidylcholine - Phospolipon 90G. The obtained results showed significant prolongation of the oxidative stability of extra virgin olive oil enriched with encapsulated blueberry phenolic extracts than with native phenolic extracts, regardless of the method used for blueberry processing. Phenolic extracts encapsulated in microemulsions had a stronger effect on the prolongation of olive oil oxidative stability in comparison with the extracts encapsulated in liposomes. The average prolongation rate of oxidative stability was 45.65% by phenolic extracts encapsulated in microemulsions prepared by mechanical homogenization (p=0.012), and 58.72% by microemulsions prepared by ultrasound homogenization (p=0.011). Phenolic extracts encapsulated in microemulsions prepared by high pressure homogenization had no effect on oil oxidative stability prolongation.
PB  - De Gruyter Poland Sp Zoo, Warsaw
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability
EP  - 33
IS  - 1
SP  - 23
VL  - 69
DO  - 10.31883/pjfns-2019-0003
ER  - 

@article{
author = "Liović, Nikolina and Bošković, Perica and Drvenica, Ivana and Jambrak, Anet Rezek and Dropulić, Ana Marija and Kresić, Greta and Nedović, Viktor and Bugarski, Branko and Zorić, Zoran and Pedisić, Sandra and Bilusić, Tea",
year = "2019",
abstract = "Natural phenolic compounds are recognized as bioactive ingredients in food but can also have a role as effective alternatives to synthetic antioxidants in stability improvement of foods prone to oxidation, such as edible oils. This study aimed at the preparation and HPLC-DAD characterization of phenolic extracts from lisccinium corymbosum L. (raw, pasteurized, freeze-dried and treated with high-intensity ultrasound), and at testing their antioxidant potential in the prevention of olive oil oxidation in the native state and encapsulated into microemulsions and liposomes systems. Water-in-oil structured microemulsions used in this study were prepared using mechanical, ultrasonic, and high pressure homogenization. Liposomes with the average size of 589.1 +/- 2.9 nm were produced with the proliposome method using commercially available phosphatidylcholine - Phospolipon 90G. The obtained results showed significant prolongation of the oxidative stability of extra virgin olive oil enriched with encapsulated blueberry phenolic extracts than with native phenolic extracts, regardless of the method used for blueberry processing. Phenolic extracts encapsulated in microemulsions had a stronger effect on the prolongation of olive oil oxidative stability in comparison with the extracts encapsulated in liposomes. The average prolongation rate of oxidative stability was 45.65% by phenolic extracts encapsulated in microemulsions prepared by mechanical homogenization (p=0.012), and 58.72% by microemulsions prepared by ultrasound homogenization (p=0.011). Phenolic extracts encapsulated in microemulsions prepared by high pressure homogenization had no effect on oil oxidative stability prolongation.",
publisher = "De Gruyter Poland Sp Zoo, Warsaw",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability",
pages = "33-23",
number = "1",
volume = "69",
doi = "10.31883/pjfns-2019-0003"
}

Liović, N., Bošković, P., Drvenica, I., Jambrak, A. R., Dropulić, A. M., Kresić, G., Nedović, V., Bugarski, B., Zorić, Z., Pedisić, S.,& Bilusić, T.. (2019). Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability. in Polish Journal of Food and Nutrition Sciences
De Gruyter Poland Sp Zoo, Warsaw., 69(1), 23-33.
https://doi.org/10.31883/pjfns-2019-0003

Liović N, Bošković P, Drvenica I, Jambrak AR, Dropulić AM, Kresić G, Nedović V, Bugarski B, Zorić Z, Pedisić S, Bilusić T. Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability. in Polish Journal of Food and Nutrition Sciences. 2019;69(1):23-33.
doi:10.31883/pjfns-2019-0003 .

Liović, Nikolina, Bošković, Perica, Drvenica, Ivana, Jambrak, Anet Rezek, Dropulić, Ana Marija, Kresić, Greta, Nedović, Viktor, Bugarski, Branko, Zorić, Zoran, Pedisić, Sandra, Bilusić, Tea, "Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability" in Polish Journal of Food and Nutrition Sciences, 69, no. 1 (2019):23-33,
https://doi.org/10.31883/pjfns-2019-0003 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić-Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3761
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine and Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić-Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine and Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić-Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić-Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine and Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić-Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine and Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Jakovetić Tanasković, Sonja
AU  - Jokić, Bojan
AU  - Grbavčić, Sanja
AU  - Drvenica, Ivana
AU  - Prlainović, Nevena
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3680
AB  - Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Clay Science
T1  - Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
EP  - 111
SP  - 103
VL  - 135
DO  - 10.1016/j.clay.2016.09.011
ER  - 

@article{
author = "Jakovetić Tanasković, Sonja and Jokić, Bojan and Grbavčić, Sanja and Drvenica, Ivana and Prlainović, Nevena and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2017",
abstract = "Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Clay Science",
title = "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants",
pages = "111-103",
volume = "135",
doi = "10.1016/j.clay.2016.09.011"
}

Jakovetić Tanasković, S., Jokić, B., Grbavčić, S., Drvenica, I., Prlainović, N., Luković, N.,& Knežević-Jugović, Z.. (2017). Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science
Elsevier Science Bv, Amsterdam., 135, 103-111.
https://doi.org/10.1016/j.clay.2016.09.011

Jakovetić Tanasković S, Jokić B, Grbavčić S, Drvenica I, Prlainović N, Luković N, Knežević-Jugović Z. Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science. 2017;135:103-111.
doi:10.1016/j.clay.2016.09.011 .

Jakovetić Tanasković, Sonja, Jokić, Bojan, Grbavčić, Sanja, Drvenica, Ivana, Prlainović, Nevena, Luković, Nevena, Knežević-Jugović, Zorica, "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants" in Applied Clay Science, 135 (2017):103-111,
https://doi.org/10.1016/j.clay.2016.09.011 . .

TY  - JOUR
AU  - Bošković, Perica
AU  - Mrduljas, Nikolina
AU  - Jambrak, Anet Rezek
AU  - Drvenica, Ivana
AU  - Kresić, Greta
AU  - BeluZić, Robert
AU  - Bugarski, Branko
AU  - Bilusić, Tea
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3597
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes
EP  - S72
SP  - S72
VL  - 256
DO  - 10.1016/j.jbiotec.2017.06.1046
ER  - 

@article{
author = "Bošković, Perica and Mrduljas, Nikolina and Jambrak, Anet Rezek and Drvenica, Ivana and Kresić, Greta and BeluZić, Robert and Bugarski, Branko and Bilusić, Tea",
year = "2017",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes",
pages = "S72-S72",
volume = "256",
doi = "10.1016/j.jbiotec.2017.06.1046"
}

Bošković, P., Mrduljas, N., Jambrak, A. R., Drvenica, I., Kresić, G., BeluZić, R., Bugarski, B.,& Bilusić, T.. (2017). Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 256, S72-S72.
https://doi.org/10.1016/j.jbiotec.2017.06.1046

Bošković P, Mrduljas N, Jambrak AR, Drvenica I, Kresić G, BeluZić R, Bugarski B, Bilusić T. Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes. in Journal of Biotechnology. 2017;256:S72-S72.
doi:10.1016/j.jbiotec.2017.06.1046 .

Bošković, Perica, Mrduljas, Nikolina, Jambrak, Anet Rezek, Drvenica, Ivana, Kresić, Greta, BeluZić, Robert, Bugarski, Branko, Bilusić, Tea, "Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes" in Journal of Biotechnology, 256 (2017):S72-S72,
https://doi.org/10.1016/j.jbiotec.2017.06.1046 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Jovanić, Svetlana
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Ilić, Vesna Lj.
AU  - Rabasović, Mihailo D.
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Bugarski, Branko
AU  - Krmpot, Aleksandar
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3747
AB  - The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.
PB  - Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham
T2  - Journal of Biomedical Optics
T1  - Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy
IS  - 2
VL  - 22
DO  - 10.1117/1.JBO.22.2.026003
ER  - 

@article{
author = "Bukara, Katarina and Jovanić, Svetlana and Drvenica, Ivana and Stančić, Ana and Ilić, Vesna Lj. and Rabasović, Mihailo D. and Pantelić, Dejan and Jelenković, Branislav and Bugarski, Branko and Krmpot, Aleksandar",
year = "2017",
abstract = "The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.",
publisher = "Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham",
journal = "Journal of Biomedical Optics",
title = "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy",
number = "2",
volume = "22",
doi = "10.1117/1.JBO.22.2.026003"
}

Bukara, K., Jovanić, S., Drvenica, I., Stančić, A., Ilić, V. Lj., Rabasović, M. D., Pantelić, D., Jelenković, B., Bugarski, B.,& Krmpot, A.. (2017). Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics
Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham., 22(2).
https://doi.org/10.1117/1.JBO.22.2.026003

Bukara K, Jovanić S, Drvenica I, Stančić A, Ilić VL, Rabasović MD, Pantelić D, Jelenković B, Bugarski B, Krmpot A. Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics. 2017;22(2).
doi:10.1117/1.JBO.22.2.026003 .

Bukara, Katarina, Jovanić, Svetlana, Drvenica, Ivana, Stančić, Ana, Ilić, Vesna Lj., Rabasović, Mihailo D., Pantelić, Dejan, Jelenković, Branislav, Bugarski, Branko, Krmpot, Aleksandar, "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy" in Journal of Biomedical Optics, 22, no. 2 (2017),
https://doi.org/10.1117/1.JBO.22.2.026003 . .

TY  - JOUR
AU  - Balanč, Bojana
AU  - Kalušević, Ana
AU  - Drvenica, Ivana
AU  - Coelho, Maria Teresa
AU  - Đorđević, Verica
AU  - Alves, Vitor D.
AU  - Sousa, Isabel
AU  - Moldao-Martins, Margarida
AU  - Rakić, Vesna M.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3376
AB  - Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.
PB  - Wiley, Hoboken
T2  - Journal of Food Science
T1  - Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract
EP  - E75
IS  - 1
SP  - E65
VL  - 81
DO  - 10.1111/1750-3841.13167
ER  - 

@article{
author = "Balanč, Bojana and Kalušević, Ana and Drvenica, Ivana and Coelho, Maria Teresa and Đorđević, Verica and Alves, Vitor D. and Sousa, Isabel and Moldao-Martins, Margarida and Rakić, Vesna M. and Nedović, Viktor and Bugarski, Branko",
year = "2016",
abstract = "Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Science",
title = "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract",
pages = "E75-E65",
number = "1",
volume = "81",
doi = "10.1111/1750-3841.13167"
}

Balanč, B., Kalušević, A., Drvenica, I., Coelho, M. T., Đorđević, V., Alves, V. D., Sousa, I., Moldao-Martins, M., Rakić, V. M., Nedović, V.,& Bugarski, B.. (2016). Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science
Wiley, Hoboken., 81(1), E65-E75.
https://doi.org/10.1111/1750-3841.13167

Balanč B, Kalušević A, Drvenica I, Coelho MT, Đorđević V, Alves VD, Sousa I, Moldao-Martins M, Rakić VM, Nedović V, Bugarski B. Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science. 2016;81(1):E65-E75.
doi:10.1111/1750-3841.13167 .

Balanč, Bojana, Kalušević, Ana, Drvenica, Ivana, Coelho, Maria Teresa, Đorđević, Verica, Alves, Vitor D., Sousa, Isabel, Moldao-Martins, Margarida, Rakić, Vesna M., Nedović, Viktor, Bugarski, Branko, "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract" in Journal of Food Science, 81, no. 1 (2016):E65-E75,
https://doi.org/10.1111/1750-3841.13167 . .

TY  - JOUR
AU  - Sekularac, Gavrilo
AU  - Kosević, Milica
AU  - Drvenica, Ivana
AU  - Dekanski, Aleksandar
AU  - Panić, Vladimir
AU  - Nikolić, Branislav
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5847
AB  - Ruthenium oxide coating on titanium was prepared by the sol-gel procedure from well-defined colloidal oxide dispersions synthesized by the microwave (MW)-assisted hydrothermal route under defined temperature and pressure heating conditions. The dispersions were characterized by dynamic light scattering (DLS) measurements and scanning electron microscopy (SEM). The electrochemical properties were analyzed as capacitive performances gained by cyclic voltammetry and electrochemical impedance spectroscopy and as the electrocatalytic activity for oxygen evolution from acid solution. The obtained dispersions were polydisperse and contained regular particles and agglomerates of increasing surface energy and decreasing particle size as the MW-assisted heating conditions were intensified. Owing to these features of the precursor dispersions, the obtained coatings had considerably improved capacitive performances and good electrocatalytic activity for oxygen evolution at high overpotentials.
PB  - Springer, New York
T2  - Journal of Solid State Electrochemistry
T1  - Titanium coated with high-performance nanocrystalline ruthenium oxide synthesized by the microwave-assisted sol-gel procedure
EP  - 3123
IS  - 11
SP  - 3115
VL  - 20
DO  - 10.1007/s10008-016-3343-z
ER  - 

@article{
author = "Sekularac, Gavrilo and Kosević, Milica and Drvenica, Ivana and Dekanski, Aleksandar and Panić, Vladimir and Nikolić, Branislav",
year = "2016",
abstract = "Ruthenium oxide coating on titanium was prepared by the sol-gel procedure from well-defined colloidal oxide dispersions synthesized by the microwave (MW)-assisted hydrothermal route under defined temperature and pressure heating conditions. The dispersions were characterized by dynamic light scattering (DLS) measurements and scanning electron microscopy (SEM). The electrochemical properties were analyzed as capacitive performances gained by cyclic voltammetry and electrochemical impedance spectroscopy and as the electrocatalytic activity for oxygen evolution from acid solution. The obtained dispersions were polydisperse and contained regular particles and agglomerates of increasing surface energy and decreasing particle size as the MW-assisted heating conditions were intensified. Owing to these features of the precursor dispersions, the obtained coatings had considerably improved capacitive performances and good electrocatalytic activity for oxygen evolution at high overpotentials.",
publisher = "Springer, New York",
journal = "Journal of Solid State Electrochemistry",
title = "Titanium coated with high-performance nanocrystalline ruthenium oxide synthesized by the microwave-assisted sol-gel procedure",
pages = "3123-3115",
number = "11",
volume = "20",
doi = "10.1007/s10008-016-3343-z"
}

Sekularac, G., Kosević, M., Drvenica, I., Dekanski, A., Panić, V.,& Nikolić, B.. (2016). Titanium coated with high-performance nanocrystalline ruthenium oxide synthesized by the microwave-assisted sol-gel procedure. in Journal of Solid State Electrochemistry
Springer, New York., 20(11), 3115-3123.
https://doi.org/10.1007/s10008-016-3343-z

Sekularac G, Kosević M, Drvenica I, Dekanski A, Panić V, Nikolić B. Titanium coated with high-performance nanocrystalline ruthenium oxide synthesized by the microwave-assisted sol-gel procedure. in Journal of Solid State Electrochemistry. 2016;20(11):3115-3123.
doi:10.1007/s10008-016-3343-z .

Sekularac, Gavrilo, Kosević, Milica, Drvenica, Ivana, Dekanski, Aleksandar, Panić, Vladimir, Nikolić, Branislav, "Titanium coated with high-performance nanocrystalline ruthenium oxide synthesized by the microwave-assisted sol-gel procedure" in Journal of Solid State Electrochemistry, 20, no. 11 (2016):3115-3123,
https://doi.org/10.1007/s10008-016-3343-z . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bukara, Katarina
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica
AU  - Veljović, Đorđe
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3329
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
EP  - 1055
IS  - 4
SP  - 1046
VL  - 32
DO  - 10.1002/btpr.2304
ER  - 

@article{
author = "Drvenica, Ivana and Bukara, Katarina and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš B. and Đorđević, Verica and Veljović, Đorđe and Belić, Aleksandar and Bugarski, Branko",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
pages = "1055-1046",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304"
}

Drvenica, I., Bukara, K., Ilić, V. Lj., Mišić, D., Vasić, B., Gajić, R. B., Đorđević, V., Veljović, Đ., Belić, A.,& Bugarski, B.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress
Wiley, Hoboken., 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica I, Bukara K, Ilić VL, Mišić D, Vasić B, Gajić RB, Đorđević V, Veljović Đ, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana, Bukara, Katarina, Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav, Gajić, Radoš B., Đorđević, Verica, Veljović, Đorđe, Belić, Aleksandar, Bugarski, Branko, "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna Lj.
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3318
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
EP  - 22
SP  - 14
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna Lj. and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
pages = "22-14",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017"
}

Bukara, K., Drvenica, I., Ilić, V. Lj., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić VL, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna Lj., Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Đorđević, Verica
AU  - Balanč, Bojana
AU  - Belščak-Cvitanović, Ana
AU  - Lević, Steva
AU  - Trifković, Kata T.
AU  - Kalušević, Ana
AU  - Kostić, Ivana
AU  - Komes, Drazenka
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3070
AB  - The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.
PB  - Springer, New York
T2  - Food Engineering Reviews
T1  - Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds
EP  - 490
IS  - 4
SP  - 452
VL  - 7
DO  - 10.1007/s12393-014-9106-7
ER  - 

@article{
author = "Đorđević, Verica and Balanč, Bojana and Belščak-Cvitanović, Ana and Lević, Steva and Trifković, Kata T. and Kalušević, Ana and Kostić, Ivana and Komes, Drazenka and Bugarski, Branko and Nedović, Viktor",
year = "2015",
abstract = "The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.",
publisher = "Springer, New York",
journal = "Food Engineering Reviews",
title = "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds",
pages = "490-452",
number = "4",
volume = "7",
doi = "10.1007/s12393-014-9106-7"
}

Đorđević, V., Balanč, B., Belščak-Cvitanović, A., Lević, S., Trifković, K. T., Kalušević, A., Kostić, I., Komes, D., Bugarski, B.,& Nedović, V.. (2015). Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews
Springer, New York., 7(4), 452-490.
https://doi.org/10.1007/s12393-014-9106-7

Đorđević V, Balanč B, Belščak-Cvitanović A, Lević S, Trifković KT, Kalušević A, Kostić I, Komes D, Bugarski B, Nedović V. Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews. 2015;7(4):452-490.
doi:10.1007/s12393-014-9106-7 .

Đorđević, Verica, Balanč, Bojana, Belščak-Cvitanović, Ana, Lević, Steva, Trifković, Kata T., Kalušević, Ana, Kostić, Ivana, Komes, Drazenka, Bugarski, Branko, Nedović, Viktor, "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds" in Food Engineering Reviews, 7, no. 4 (2015):452-490,
https://doi.org/10.1007/s12393-014-9106-7 . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Đurić, Zorka Ž.
AU  - Draškovič, Petra
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2900
AB  - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
AB  - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
T1  - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
EP  - 76
IS  - 1
SP  - 67
VL  - 69
DO  - 10.2298/HEMIND140124021K
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bukara, Katarina and Mojsilović, Slavko B. and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko",
year = "2015",
abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova",
pages = "76-67",
number = "1",
volume = "69",
doi = "10.2298/HEMIND140124021K"
}

Kostić, I. T., Ilić, V. Lj., Bukara, K., Mojsilović, S. B., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(1), 67-76.
https://doi.org/10.2298/HEMIND140124021K

Kostić IT, Ilić VL, Bukara K, Mojsilović SB, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76.
doi:10.2298/HEMIND140124021K .

Kostić, Ivana T., Ilić, Vesna Lj., Bukara, Katarina, Mojsilović, Slavko B., Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76,
https://doi.org/10.2298/HEMIND140124021K . .

TY  - JOUR
AU  - Dimić, Ivana
AU  - Cvijović-Alagić, Ivana
AU  - Kostić, Ivana T.
AU  - Perić-Grujić, Aleksandra
AU  - Rakin, Marko
AU  - Putić, Slaviša
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2625
AB  - Metallic biomaterials, which are mainly used for the damaged hard tissue replacements, are materials with high strength, excellent toughness and good wear resistance. The disadvantages of metals as implant materials are their susceptibility to corrosion, the elastic modulus mismatch between metals and human hard tissues, relatively high density and metallic ion release, which can cause serious health problems. The aim of this study was to examine metallic ion release from Co-Cr-Mo alloy in artificial saliva. In that purpose, alloy samples were immersed into artificial saliva with different pH values (4.0, 5.5 and 7.5). After a certain immersion period (1, 3 and 6 weeks) the concentrations of released ions were determined using inductively coupled plasma-mass spectrometry (ICP-MS). The research findings were used in order to define the dependence between the concentration of released metallic ions, artificial saliva pH values and immersion time. The determined released metallic ions concentrations were compared with literature data in order to describe and better understand the phenomenon of metallic ion release from the biocompatible cobalt-based alloy.
AB  - Metalni biomaterijali, koji se najčešće koriste za zamenu oštećenih čvrstih tkiva u organizmu čoveka, su materijali velike čvrstoće, žilavosti i otpornosti prema habanju. Nedostaci metala, kao implantnih materijala, su njihova podložnost koroziji, neusklađenost modula elastičnosti metala i čvrstih ljudskih tkiva, velika gustina i otpuštanje metalnih jona koje može da izazove ozbiljne zdravstvene probleme. Cilj ovog rada je bio da se ispita otpuštanje metalnih jona iz Co-Cr-Mo legure u rastvoru veštačke pljuvačke. Uzorci legure su potopljeni u rastvor veštačke pljuvačke različite pH vrednosti (4,0, 5,5 i 7,5). Nakon određenog vremena izlaganja legure veštačkoj pljuvačci (1, 3 i 6 nedelja) određene su koncentracije otpuštenih jona primenom indukcije spregnute plazme sa masenom spektrometrijom (ICP-MS). Dobijeni rezultati su iskorišćeni u cilju definisanja zavisnosti između koncentracije otpuštenih jona, pH vrednosti veštačke pljuvačke i dužine potapanja legure u rastvor veštačke pljuvačke. Osim toga, utvrđene koncentracije otpuštenih metalnih jona iz ispitivane legure su upoređene sa podacima dostupnim u literaturi u cilju što boljeg opisivanja i razumevanja fenomena otpuštanja metalnih jona iz biokompatibilne Co-Cr-Mo legure.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Metallic ion release from biocompatible cobalt-based alloy
T1  - Otpuštanje metalnih jona iz biokompatibilne legure kobalta
EP  - 577
IS  - 4
SP  - 571
VL  - 20
DO  - 10.2298/CICEQ130813039D
ER  - 

@article{
author = "Dimić, Ivana and Cvijović-Alagić, Ivana and Kostić, Ivana T. and Perić-Grujić, Aleksandra and Rakin, Marko and Putić, Slaviša and Bugarski, Branko",
year = "2014",
abstract = "Metallic biomaterials, which are mainly used for the damaged hard tissue replacements, are materials with high strength, excellent toughness and good wear resistance. The disadvantages of metals as implant materials are their susceptibility to corrosion, the elastic modulus mismatch between metals and human hard tissues, relatively high density and metallic ion release, which can cause serious health problems. The aim of this study was to examine metallic ion release from Co-Cr-Mo alloy in artificial saliva. In that purpose, alloy samples were immersed into artificial saliva with different pH values (4.0, 5.5 and 7.5). After a certain immersion period (1, 3 and 6 weeks) the concentrations of released ions were determined using inductively coupled plasma-mass spectrometry (ICP-MS). The research findings were used in order to define the dependence between the concentration of released metallic ions, artificial saliva pH values and immersion time. The determined released metallic ions concentrations were compared with literature data in order to describe and better understand the phenomenon of metallic ion release from the biocompatible cobalt-based alloy., Metalni biomaterijali, koji se najčešće koriste za zamenu oštećenih čvrstih tkiva u organizmu čoveka, su materijali velike čvrstoće, žilavosti i otpornosti prema habanju. Nedostaci metala, kao implantnih materijala, su njihova podložnost koroziji, neusklađenost modula elastičnosti metala i čvrstih ljudskih tkiva, velika gustina i otpuštanje metalnih jona koje može da izazove ozbiljne zdravstvene probleme. Cilj ovog rada je bio da se ispita otpuštanje metalnih jona iz Co-Cr-Mo legure u rastvoru veštačke pljuvačke. Uzorci legure su potopljeni u rastvor veštačke pljuvačke različite pH vrednosti (4,0, 5,5 i 7,5). Nakon određenog vremena izlaganja legure veštačkoj pljuvačci (1, 3 i 6 nedelja) određene su koncentracije otpuštenih jona primenom indukcije spregnute plazme sa masenom spektrometrijom (ICP-MS). Dobijeni rezultati su iskorišćeni u cilju definisanja zavisnosti između koncentracije otpuštenih jona, pH vrednosti veštačke pljuvačke i dužine potapanja legure u rastvor veštačke pljuvačke. Osim toga, utvrđene koncentracije otpuštenih metalnih jona iz ispitivane legure su upoređene sa podacima dostupnim u literaturi u cilju što boljeg opisivanja i razumevanja fenomena otpuštanja metalnih jona iz biokompatibilne Co-Cr-Mo legure.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Metallic ion release from biocompatible cobalt-based alloy, Otpuštanje metalnih jona iz biokompatibilne legure kobalta",
pages = "577-571",
number = "4",
volume = "20",
doi = "10.2298/CICEQ130813039D"
}

Dimić, I., Cvijović-Alagić, I., Kostić, I. T., Perić-Grujić, A., Rakin, M., Putić, S.,& Bugarski, B.. (2014). Metallic ion release from biocompatible cobalt-based alloy. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 20(4), 571-577.
https://doi.org/10.2298/CICEQ130813039D

Dimić I, Cvijović-Alagić I, Kostić IT, Perić-Grujić A, Rakin M, Putić S, Bugarski B. Metallic ion release from biocompatible cobalt-based alloy. in Chemical Industry & Chemical Engineering Quarterly. 2014;20(4):571-577.
doi:10.2298/CICEQ130813039D .

Dimić, Ivana, Cvijović-Alagić, Ivana, Kostić, Ivana T., Perić-Grujić, Aleksandra, Rakin, Marko, Putić, Slaviša, Bugarski, Branko, "Metallic ion release from biocompatible cobalt-based alloy" in Chemical Industry & Chemical Engineering Quarterly, 20, no. 4 (2014):571-577,
https://doi.org/10.2298/CICEQ130813039D . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Đorđević, Verica
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko B.
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Veljović, Đorđe
AU  - Misić, Danijela M.
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2738
AB  - The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
EP  - 259
SP  - 250
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Đorđević, Verica and Bukara, Katarina and Mojsilović, Slavko B. and Nedović, Viktor and Bugarski, Diana and Veljović, Đorđe and Misić, Danijela M. and Bugarski, Branko",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization",
pages = "259-250",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043"
}

Kostić, I. T., Ilić, V. Lj., Đorđević, V., Bukara, K., Mojsilović, S. B., Nedović, V., Bugarski, D., Veljović, Đ., Misić, D. M.,& Bugarski, B.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostić IT, Ilić VL, Đorđević V, Bukara K, Mojsilović SB, Nedović V, Bugarski D, Veljović Đ, Misić DM, Bugarski B. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids and Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostić, Ivana T., Ilić, Vesna Lj., Đorđević, Verica, Bukara, Katarina, Mojsilović, Slavko B., Nedović, Viktor, Bugarski, Diana, Veljović, Đorđe, Misić, Danijela M., Bugarski, Branko, "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization" in Colloids and Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .

TY  - JOUR
AU  - Lević, Steva
AU  - Obradović, Nina
AU  - Pavlović, Vladimir B.
AU  - Balanč, Bojana
AU  - Kostić, Ivana
AU  - Mitrić, Miodrag
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2745
AB  - In this study, polyvinyl alcohol (PVA) nanofibers with ethyl vanillin as an active compound were prepared using electrospinning technique. The final products of electrospinning process were in the form of nanofibers films. PVA/ethyl vanillin nanofibers, having fibers diameters in the range 100-1700 nm, were successfully electrospun from ethanol/water mixture of PVA and ethyl vanillin. The effects of immobilization process on ethyl vanillin thermal properties were investigated by differential scanning calorimetry (DSC). The results of DSC showed significant influence of immobilization process on thermal properties of ethyl vanillin. It was noticed that melting point of immobilized ethyl vanillin was lower (similar to 55 A degrees C) compared to free flavor (similar to 77 A degrees C). Our results showed that films based on PVA/ethyl vanillin nanofibers are mechanically stable.
PB  - Springer, Dordrecht
T2  - Journal of Thermal Analysis and Calorimetry
T1  - Thermal, morphological, and mechanical properties of ethyl vanillin immobilized in polyvinyl alcohol by electrospinning process
EP  - 668
IS  - 2
SP  - 661
VL  - 118
DO  - 10.1007/s10973-014-4060-4
ER  - 

@article{
author = "Lević, Steva and Obradović, Nina and Pavlović, Vladimir B. and Balanč, Bojana and Kostić, Ivana and Mitrić, Miodrag and Bugarski, Branko and Nedović, Viktor",
year = "2014",
abstract = "In this study, polyvinyl alcohol (PVA) nanofibers with ethyl vanillin as an active compound were prepared using electrospinning technique. The final products of electrospinning process were in the form of nanofibers films. PVA/ethyl vanillin nanofibers, having fibers diameters in the range 100-1700 nm, were successfully electrospun from ethanol/water mixture of PVA and ethyl vanillin. The effects of immobilization process on ethyl vanillin thermal properties were investigated by differential scanning calorimetry (DSC). The results of DSC showed significant influence of immobilization process on thermal properties of ethyl vanillin. It was noticed that melting point of immobilized ethyl vanillin was lower (similar to 55 A degrees C) compared to free flavor (similar to 77 A degrees C). Our results showed that films based on PVA/ethyl vanillin nanofibers are mechanically stable.",
publisher = "Springer, Dordrecht",
journal = "Journal of Thermal Analysis and Calorimetry",
title = "Thermal, morphological, and mechanical properties of ethyl vanillin immobilized in polyvinyl alcohol by electrospinning process",
pages = "668-661",
number = "2",
volume = "118",
doi = "10.1007/s10973-014-4060-4"
}

Lević, S., Obradović, N., Pavlović, V. B., Balanč, B., Kostić, I., Mitrić, M., Bugarski, B.,& Nedović, V.. (2014). Thermal, morphological, and mechanical properties of ethyl vanillin immobilized in polyvinyl alcohol by electrospinning process. in Journal of Thermal Analysis and Calorimetry
Springer, Dordrecht., 118(2), 661-668.
https://doi.org/10.1007/s10973-014-4060-4

Lević S, Obradović N, Pavlović VB, Balanč B, Kostić I, Mitrić M, Bugarski B, Nedović V. Thermal, morphological, and mechanical properties of ethyl vanillin immobilized in polyvinyl alcohol by electrospinning process. in Journal of Thermal Analysis and Calorimetry. 2014;118(2):661-668.
doi:10.1007/s10973-014-4060-4 .

Lević, Steva, Obradović, Nina, Pavlović, Vladimir B., Balanč, Bojana, Kostić, Ivana, Mitrić, Miodrag, Bugarski, Branko, Nedović, Viktor, "Thermal, morphological, and mechanical properties of ethyl vanillin immobilized in polyvinyl alcohol by electrospinning process" in Journal of Thermal Analysis and Calorimetry, 118, no. 2 (2014):661-668,
https://doi.org/10.1007/s10973-014-4060-4 . .

TY  - JOUR
AU  - Balanč, Bojana
AU  - Kostić, Ivana T.
AU  - Zvonar, Alenka
AU  - Đorđević, Verica
AU  - Gasperlin, Mirjana
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2387
AB  - Several different methods for production of liposomes incorporating resveratrol were investigated and compared from the aspect of size distribution, surface charge, entrapment efficiency, phase behavior and stability. Thin film method and proliposome method provided high entrapment efficiency (92.9% and 97.4%, respectively). Extrusion and sonication techniques were applied to obtain particles of the average diameter between 120 and 270 nm. The sonicated liposomes incorporated resveratrol (44-56%) fewer than extruded vesicles (92-96%). Antioxidative activity of resveratrol was retained upon encapsulation. Differential scanning calorimetry was performed in order to study the interaction of liposomal membranes with resveratrol, and their physical state. The release studies performed in Franz diffusion cell showed that liposomes impart slow diffusion of resveratrol, where diffusion resistance derived from liposomal membrane ranged from 5.90 . 10(5) to 9.55 . 10(5) s/m depending on the size of particles. Cytotoxicity of the formulations was evaluated via morphological changes of keratinocytes treated by liposomes. Industrial Relevance: Resveratrol displays many health-beneficial properties and possesses a remarkably strong antioxidant activity. Although often consumed in food, the positive effects of resveratrol are restricted because it is prone to oxidation, poorly absorbed when orally administrated, and cytotoxic in higher total dosages (though relatively high local concentrations are required for an effect). Encapsulation is one way to improve bioavailability and stability of resveratrol; herein the main challenge is to find a suitable solution, as resveratrol is weakly water soluble. This has motivated us to design new formulations based on liposomes for delivering of resveratrol. In the food sector, liposomes have been investigated for delivering proteins, enzymes, antioxidants, flavors and vitamins. The mean advantage of liposomes over other encapsulation technologies (spray-drying, extrusion, and fluidized beds) is the stability that liposomes impart to water-soluble compounds in aqueous surroundings. Liposomes are able to stabilize the encapsulated materials against a range of environmental and chemical changes. Another important characteristic of liposomes is that, unlike many other existing encapsulants, they can be utilized in the entrapment, delivery, and release of poorly water soluble compounds, such as resveratrol, and they are also convenient for water-soluble, lipid-soluble, and amphiphilic compounds. As liposomes could be produced from naturally occurring components, regulatory issues that may prevent the application in food systems are potentially diminished, and new formulations could be quickly implemented. Despite benefits described here, up to date little use of liposomes in food systems has been made, as current manufacturing processes are mainly time consuming, often consisting of several steps with high costs of raw materials. Another problem is that devices available commercially which are utilized for production of liposomes are able to process only small quantities. Therefore, our research is devoted to the development of the process for liposome production which is easy to scale up, and at the same time, is effective as the common way based on thin film hydration process. The process elaborated in our study utilizes a commercial lipid mixture. The method used called proliposome method is based on replacement of ethanol solvent by aqueous media. For liposome downsizing, sonication (which can be easily modified to increase sample volume capability) is tested versus membrane extrusion (equipment for small-large batches is readily available). The goal of this article is to provide evidence for food manufacturers and food scientists to make broader use of resveratrol-loaded liposomes that can add value and improve the quality of existing food products.
PB  - Elsevier Sci Ltd, Oxford
T2  - Innovative Food Science & Emerging Technologies
T1  - Resveratrol loaded liposomes produced by different techniques
EP  - 189
SP  - 181
VL  - 19
DO  - 10.1016/j.ifset.2013.03.006
ER  - 

@article{
author = "Balanč, Bojana and Kostić, Ivana T. and Zvonar, Alenka and Đorđević, Verica and Gasperlin, Mirjana and Nedović, Viktor and Bugarski, Branko",
year = "2013",
abstract = "Several different methods for production of liposomes incorporating resveratrol were investigated and compared from the aspect of size distribution, surface charge, entrapment efficiency, phase behavior and stability. Thin film method and proliposome method provided high entrapment efficiency (92.9% and 97.4%, respectively). Extrusion and sonication techniques were applied to obtain particles of the average diameter between 120 and 270 nm. The sonicated liposomes incorporated resveratrol (44-56%) fewer than extruded vesicles (92-96%). Antioxidative activity of resveratrol was retained upon encapsulation. Differential scanning calorimetry was performed in order to study the interaction of liposomal membranes with resveratrol, and their physical state. The release studies performed in Franz diffusion cell showed that liposomes impart slow diffusion of resveratrol, where diffusion resistance derived from liposomal membrane ranged from 5.90 . 10(5) to 9.55 . 10(5) s/m depending on the size of particles. Cytotoxicity of the formulations was evaluated via morphological changes of keratinocytes treated by liposomes. Industrial Relevance: Resveratrol displays many health-beneficial properties and possesses a remarkably strong antioxidant activity. Although often consumed in food, the positive effects of resveratrol are restricted because it is prone to oxidation, poorly absorbed when orally administrated, and cytotoxic in higher total dosages (though relatively high local concentrations are required for an effect). Encapsulation is one way to improve bioavailability and stability of resveratrol; herein the main challenge is to find a suitable solution, as resveratrol is weakly water soluble. This has motivated us to design new formulations based on liposomes for delivering of resveratrol. In the food sector, liposomes have been investigated for delivering proteins, enzymes, antioxidants, flavors and vitamins. The mean advantage of liposomes over other encapsulation technologies (spray-drying, extrusion, and fluidized beds) is the stability that liposomes impart to water-soluble compounds in aqueous surroundings. Liposomes are able to stabilize the encapsulated materials against a range of environmental and chemical changes. Another important characteristic of liposomes is that, unlike many other existing encapsulants, they can be utilized in the entrapment, delivery, and release of poorly water soluble compounds, such as resveratrol, and they are also convenient for water-soluble, lipid-soluble, and amphiphilic compounds. As liposomes could be produced from naturally occurring components, regulatory issues that may prevent the application in food systems are potentially diminished, and new formulations could be quickly implemented. Despite benefits described here, up to date little use of liposomes in food systems has been made, as current manufacturing processes are mainly time consuming, often consisting of several steps with high costs of raw materials. Another problem is that devices available commercially which are utilized for production of liposomes are able to process only small quantities. Therefore, our research is devoted to the development of the process for liposome production which is easy to scale up, and at the same time, is effective as the common way based on thin film hydration process. The process elaborated in our study utilizes a commercial lipid mixture. The method used called proliposome method is based on replacement of ethanol solvent by aqueous media. For liposome downsizing, sonication (which can be easily modified to increase sample volume capability) is tested versus membrane extrusion (equipment for small-large batches is readily available). The goal of this article is to provide evidence for food manufacturers and food scientists to make broader use of resveratrol-loaded liposomes that can add value and improve the quality of existing food products.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Innovative Food Science & Emerging Technologies",
title = "Resveratrol loaded liposomes produced by different techniques",
pages = "189-181",
volume = "19",
doi = "10.1016/j.ifset.2013.03.006"
}

Balanč, B., Kostić, I. T., Zvonar, A., Đorđević, V., Gasperlin, M., Nedović, V.,& Bugarski, B.. (2013). Resveratrol loaded liposomes produced by different techniques. in Innovative Food Science & Emerging Technologies
Elsevier Sci Ltd, Oxford., 19, 181-189.
https://doi.org/10.1016/j.ifset.2013.03.006

Balanč B, Kostić IT, Zvonar A, Đorđević V, Gasperlin M, Nedović V, Bugarski B. Resveratrol loaded liposomes produced by different techniques. in Innovative Food Science & Emerging Technologies. 2013;19:181-189.
doi:10.1016/j.ifset.2013.03.006 .

Balanč, Bojana, Kostić, Ivana T., Zvonar, Alenka, Đorđević, Verica, Gasperlin, Mirjana, Nedović, Viktor, Bugarski, Branko, "Resveratrol loaded liposomes produced by different techniques" in Innovative Food Science & Emerging Technologies, 19 (2013):181-189,
https://doi.org/10.1016/j.ifset.2013.03.006 . .

TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Mojsilović, Slavko B.
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2103
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min.
AB  - Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes
T1  - Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom
EP  - 529
IS  - 4
SP  - 519
VL  - 66
DO  - 10.2298/HEMIND111122008S
ER  - 

@article{
author = "Pravilović, Radoslava and Mojsilović, Slavko B. and Kostić, Ivana T. and Ilić, Vesna Lj. and Bugarski, Diana and Đorđević, Verica and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts). Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 83% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The results show that isolation process yielded predominantly to oxyhemoglobin. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min., Dugi niz godina, i kod nas i u svetu, razvijaju se tehnološki postupci za izolovanje strukturno očuvanog i biološki aktivnog hemoglobina koji bi mogao da se koristi kao zamenik za krv, kao izvor biološki visokoaktivnog hemskog gvožđa u prevenciji anemije kod ljudi i životinja, ili kao reagens u dijagnostici. U ovom radu optimizovan je postupak za izolovanje hemoglobina iz eritrocita poreklom iz otpadne klanične goveđe krvi sa ciljem dobijanja preparata hemskog gvožđa za prevenciju anemije kod životinja. Testirana je osetljivost goveđih eritrocita na osmotsku lizu sa ciljem definisanja optimalnog puferskog sistema za efikasno izvođenje kontrolisane hemolize. Dobijeni rezultati su pokazali da goveđi eritrociti imaju povećanu osmotsku osetljivost u odnosu na humane eritrocite, a da je optimalan puferski sistem za izvođenje njihove kontrolisane hemolize 35 mM natrijum-fosfatni/NaCl pufer pH 7,2-7,4. Kontrolisana hemoliza sa optimizovanim puferskim sistemom je izvedena u membranskom reaktorskom sistemu i ostvaren je prinos hemoglobina od 83±12%. Tokom ovog procesa nije došlo do oštećenja membrane eritrocita, a intaktne membrane praznih eritrocita bi se mogle koristiti za inkapsulaciju biološki aktivnih supstanci.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes, Optimizacija procesa izolovanja hemoglobina iz goveđih eritrocita kontrolisanom hemolizom",
pages = "529-519",
number = "4",
volume = "66",
doi = "10.2298/HEMIND111122008S"
}

Pravilović, R., Mojsilović, S. B., Kostić, I. T., Ilić, V. Lj., Bugarski, D., Đorđević, V.,& Bugarski, B.. (2012). Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(4), 519-529.
https://doi.org/10.2298/HEMIND111122008S

Pravilović R, Mojsilović SB, Kostić IT, Ilić VL, Bugarski D, Đorđević V, Bugarski B. Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes. in Hemijska industrija. 2012;66(4):519-529.
doi:10.2298/HEMIND111122008S .

Pravilović, Radoslava, Mojsilović, Slavko B., Kostić, Ivana T., Ilić, Vesna Lj., Bugarski, Diana, Đorđević, Verica, Bugarski, Branko, "Optimization of gradual hemolysis for isolation of hemoglobin from bovine erythrocytes" in Hemijska industrija, 66, no. 4 (2012):519-529,
https://doi.org/10.2298/HEMIND111122008S . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2097
AB  - Significant development of cells and bioactive compound encapsulation technologies is taking place due to an exceptional possibility of their application in various scientific disciplines, including biomedicine, pharmacy, cosmetology, food and agricultural sciences, beverage production, industrial waste treatment. Despite the broad application of microencapsulation, the literature reviews on dispersion techniques for microcapsule/microbead production, their advantages, restrictions and drawbacks are scarce. The purpose of this paper is to assess the possibilities of electrostatic extrusion for encapsulation of biological material, including living cells in hydrogel microbeads. The paper presents an overview of the mechanisms of droplet formation and controlling experimental parameters for producing microbeads by means of electrostatic extrusion. Electrostatic droplet formation utilizes a special type of physical process taking advantage of electrostatic effects occurring in flowing conductive liquids after introduction of an electric field. When an electrostatic field is applied to the metal needle and an electric charge is induced in the liquid flowing out of the needle, the size of droplet detaching from the needle tip decreases as a function of applied electrostatic field. It has been shown that few parameters affect microbead size: applied voltage, electrode geometry, needle size, polarity arrangement and polymer concentration. The electrostatic droplet formation is one of the most precise methods, which enables one to produce spherical and uniform particles ranging from 100 up to 1000 μm. Most of the authors report that the encapsulated compounds (drugs, enzymes and living cells) remain unaltered after electrostatic extrusion. This technique seems to be particularly promising in biotechnology, pharmaceutical and cosmetics industries, where a low-temperature process, preserving heat-sensitive material is a prerequisite. Future efforts in developing of electrostatic extrusion should be directed towards adequately scaling-up for commercial purpose.
AB  - Uprkos širokom spektru moguće primene mikroinkapsulacije u medicini, farmaceutskoj, kozmetičkoj i prehrambenoj industriji, literaturni podaci o disperzionim tehnikama koje se koriste u te svrhe su vrlo oskudni. U ovom radu istaknut je značaj elektrostatičke ekstruzije za inkapsulaciju ćelija i bioaktivnih komponenti u hidrogel mikročestice. Elektrostatička ekstruzija je jednostavna, precizna, efikasna i ekonomski isplativa metoda, a optimizacijom nekoliko procesnih parametara moguće je dobiti uniformne čestice sa inkapsuliranim biološkim materijalom željenog prečnika (100-1000 μm). Budući napori u razvoju ove disperzione tehnike trebalo bi da budu usmereni ka uvećanju razmera procesa.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Electrostatic extrusion as a dispersion technique for encapsulation of cells and bioactive compounds
T1  - Elektrostatička ekstruzija kao disperziona tehnika za inkapsulaciju ćelija i biološki aktivnih supstanci
EP  - 517
IS  - 4
SP  - 505
VL  - 66
DO  - 10.2298/HEMIND111209013K
ER  - 

@article{
author = "Kostić, Ivana T. and Balanč, Bojana and Đorđević, Verica and Lević, Steva and Nedović, Viktor and Bugarski, Branko",
year = "2012",
abstract = "Significant development of cells and bioactive compound encapsulation technologies is taking place due to an exceptional possibility of their application in various scientific disciplines, including biomedicine, pharmacy, cosmetology, food and agricultural sciences, beverage production, industrial waste treatment. Despite the broad application of microencapsulation, the literature reviews on dispersion techniques for microcapsule/microbead production, their advantages, restrictions and drawbacks are scarce. The purpose of this paper is to assess the possibilities of electrostatic extrusion for encapsulation of biological material, including living cells in hydrogel microbeads. The paper presents an overview of the mechanisms of droplet formation and controlling experimental parameters for producing microbeads by means of electrostatic extrusion. Electrostatic droplet formation utilizes a special type of physical process taking advantage of electrostatic effects occurring in flowing conductive liquids after introduction of an electric field. When an electrostatic field is applied to the metal needle and an electric charge is induced in the liquid flowing out of the needle, the size of droplet detaching from the needle tip decreases as a function of applied electrostatic field. It has been shown that few parameters affect microbead size: applied voltage, electrode geometry, needle size, polarity arrangement and polymer concentration. The electrostatic droplet formation is one of the most precise methods, which enables one to produce spherical and uniform particles ranging from 100 up to 1000 μm. Most of the authors report that the encapsulated compounds (drugs, enzymes and living cells) remain unaltered after electrostatic extrusion. This technique seems to be particularly promising in biotechnology, pharmaceutical and cosmetics industries, where a low-temperature process, preserving heat-sensitive material is a prerequisite. Future efforts in developing of electrostatic extrusion should be directed towards adequately scaling-up for commercial purpose., Uprkos širokom spektru moguće primene mikroinkapsulacije u medicini, farmaceutskoj, kozmetičkoj i prehrambenoj industriji, literaturni podaci o disperzionim tehnikama koje se koriste u te svrhe su vrlo oskudni. U ovom radu istaknut je značaj elektrostatičke ekstruzije za inkapsulaciju ćelija i bioaktivnih komponenti u hidrogel mikročestice. Elektrostatička ekstruzija je jednostavna, precizna, efikasna i ekonomski isplativa metoda, a optimizacijom nekoliko procesnih parametara moguće je dobiti uniformne čestice sa inkapsuliranim biološkim materijalom željenog prečnika (100-1000 μm). Budući napori u razvoju ove disperzione tehnike trebalo bi da budu usmereni ka uvećanju razmera procesa.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Electrostatic extrusion as a dispersion technique for encapsulation of cells and bioactive compounds, Elektrostatička ekstruzija kao disperziona tehnika za inkapsulaciju ćelija i biološki aktivnih supstanci",
pages = "517-505",
number = "4",
volume = "66",
doi = "10.2298/HEMIND111209013K"
}

Kostić, I. T., Balanč, B., Đorđević, V., Lević, S., Nedović, V.,& Bugarski, B.. (2012). Electrostatic extrusion as a dispersion technique for encapsulation of cells and bioactive compounds. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(4), 505-517.
https://doi.org/10.2298/HEMIND111209013K

Kostić IT, Balanč B, Đorđević V, Lević S, Nedović V, Bugarski B. Electrostatic extrusion as a dispersion technique for encapsulation of cells and bioactive compounds. in Hemijska industrija. 2012;66(4):505-517.
doi:10.2298/HEMIND111209013K .

Kostić, Ivana T., Balanč, Bojana, Đorđević, Verica, Lević, Steva, Nedović, Viktor, Bugarski, Branko, "Electrostatic extrusion as a dispersion technique for encapsulation of cells and bioactive compounds" in Hemijska industrija, 66, no. 4 (2012):505-517,
https://doi.org/10.2298/HEMIND111209013K . .

TY  - JOUR
AU  - Kostić, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2063
AB  - The erythrocytes are the most abundant cellular components ( gt 99%) of blood in humans. Besides their well known physiological functions, they serve as a natural blood compartment participating in biodistribution, metabolism and action of certain drugs. On the other hand, the erythrocytes possess potential carrier capabilities and can be used for the controlled and targeted delivery of various bioactive compounds, including peptides and genetic materials. The erythrocytes feature some unique advantages compared to other delivery systems, such as high biocompatibility, biodegradability, long life­span, remarkable decrease in concentration fluctuations in steady state in comparison to the conventional methods of drug administration etc. This short review accentuate the most significant experimental results and perspectives of medical applications of novel drug delivery systems based on erythrocytes. .
AB  - Eritrociti predstavljaju najzastupljenije ćelijske komponente krvi ( gt 99%) kod ljudi. Pored dobro poznatih fizioloških funkcija koje vrše, oni predstavljaju i prirodne kompartmane krvi koje učestvuju u biodistribuciji, metabolizmu i delovanju određenih lekovitih supstanci. S druge strane, eritrociti poseduju ogroman potencijal kao nosači za kontrolisanu i ciljanu isporuku različitih bioaktivnih jedinjenja, uključujući i peptide i genetički materijal. Produženi životni vek u cirkulaciji, dostupnost, visoka biokompatibilnost i prirodni mehanizmi za bezbednu eliminaciju, kao i značajno smanjenje fluktuacije koncentracije lekovite supstance u plazmi, čine jedinstvene prednosti eritrocita kao nosača lekovitih supstanci u poređenju sa konvencionalnim načinima administracije lekova. Ovaj kratki pregled ističe najznačajnije eksperimentalne rezultate i izglede za primenu eritrocita kao savremenih nosača lekovitih supstanci u medicini. .
PB  - MOST ART d.o.o., Beograd
T2  - MD - Medical data
T1  - Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications
T1  - Eritrociti kao savremeni nosači lekovitih supstanci - trenutni status, izazovi i izgledi za primenu u medicini
EP  - 292
IS  - 3
SP  - 287
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2063
ER  - 

@article{
author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bugarski, Branko",
year = "2012",
abstract = "The erythrocytes are the most abundant cellular components ( gt 99%) of blood in humans. Besides their well known physiological functions, they serve as a natural blood compartment participating in biodistribution, metabolism and action of certain drugs. On the other hand, the erythrocytes possess potential carrier capabilities and can be used for the controlled and targeted delivery of various bioactive compounds, including peptides and genetic materials. The erythrocytes feature some unique advantages compared to other delivery systems, such as high biocompatibility, biodegradability, long life­span, remarkable decrease in concentration fluctuations in steady state in comparison to the conventional methods of drug administration etc. This short review accentuate the most significant experimental results and perspectives of medical applications of novel drug delivery systems based on erythrocytes. ., Eritrociti predstavljaju najzastupljenije ćelijske komponente krvi ( gt 99%) kod ljudi. Pored dobro poznatih fizioloških funkcija koje vrše, oni predstavljaju i prirodne kompartmane krvi koje učestvuju u biodistribuciji, metabolizmu i delovanju određenih lekovitih supstanci. S druge strane, eritrociti poseduju ogroman potencijal kao nosači za kontrolisanu i ciljanu isporuku različitih bioaktivnih jedinjenja, uključujući i peptide i genetički materijal. Produženi životni vek u cirkulaciji, dostupnost, visoka biokompatibilnost i prirodni mehanizmi za bezbednu eliminaciju, kao i značajno smanjenje fluktuacije koncentracije lekovite supstance u plazmi, čine jedinstvene prednosti eritrocita kao nosača lekovitih supstanci u poređenju sa konvencionalnim načinima administracije lekova. Ovaj kratki pregled ističe najznačajnije eksperimentalne rezultate i izglede za primenu eritrocita kao savremenih nosača lekovitih supstanci u medicini. .",
publisher = "MOST ART d.o.o., Beograd",
journal = "MD - Medical data",
title = "Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications, Eritrociti kao savremeni nosači lekovitih supstanci - trenutni status, izazovi i izgledi za primenu u medicini",
pages = "292-287",
number = "3",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2063"
}

Kostić, I. T., Ilić, V. Lj.,& Bugarski, B.. (2012). Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications. in MD - Medical data
MOST ART d.o.o., Beograd., 4(3), 287-292.
https://hdl.handle.net/21.15107/rcub_technorep_2063

Kostić IT, Ilić VL, Bugarski B. Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications. in MD - Medical data. 2012;4(3):287-292.
https://hdl.handle.net/21.15107/rcub_technorep_2063 .

Kostić, Ivana T., Ilić, Vesna Lj., Bugarski, Branko, "Erythrocytes as a novel drug delivery systems: Current status, challenges and perspectives of medical applications" in MD - Medical data, 4, no. 3 (2012):287-292,
https://hdl.handle.net/21.15107/rcub_technorep_2063 .