@conference{
author = "Jovanović, Aleksandra A. and Zdunić, Gordana and Ćujić, Nada and Šavikin, Katarina and Bukara, Katarina and Lević, Steva and Bugarski, Branko",
year = "2015",
abstract = "Wild Thyme (Thymus serpyllum, Lamiaceae) is a subshrub from the Lamiaceae family, with
plants that are rich in essential oils and antioxidative polyphenolic substances. Reducing
potential of phenolic compounds gives them a special role in the adsorption and neutralization
of free radicals. In the present study, we used a method of maceration, lasting 15, 30, 60 and 90
minutes, degree of fragmentation 0.7, two types of solvent (50% and 96% ethanol) and ratio
drugs:solvent 1:20. In Folin-Ciocalteu method, the higest total polyphenols content was obtained
in 50% ethanolic extract after 90 minutes of extraction, while the lowest total polyphenols
content was recorded in 96% ethanolic extract after 15 minutes (1173,21 mg GA/g DW and
102,56 mg GA/g DW, respectively). Ethanolic extracts were analized for antioxidant activity by
using spectrophotometric methods, DPPH method and ABTS method. There is signifficant
difference between antioxidant activity in 50% and in 96% ethanolic extracts. The worse result
was obtained with 96% ethanol as extractant after 15 minutes of maceration (IC50 31,323 mg/ml
and 0,116 mM Trolox/g DW). This study found that both total polyphenols and antioxidant
activities determined were significantly affected by the type of solvent and time of extraction.
Also, the levels of total soluble phenolic compounds were positively correlated with free radical
scavenging activities against DPPH and ABTS radicals., Majčina dušica (Thymus serpyllum, Lamiaceae) je višegodišnji busen iz familije Lamiaceae, koju
čine biljke bogate etarskim uljima i antioksidativnim polifenolnim supstancama. Antioksidativnu
aktivnost fenolne komponente duguju svom redukujućem potencijalu, koji im daje posebnu ulogu
u adsorpciji i neutralizaciji slobodnih radikala. U ovoj studiji ekstrakti T. serpyllum su dobijeni
metodom maceracije, u trajanju od 15, 30, 60 i 90 minuta, korišćenjem droge stepena
usitnjenosti 0,7, dva rastvarača (50% i 96% etanola) i odnosa droga:rastvarač 1:20. FolinCiocalteu metodom je određen sadržaj ukupnih polifenola, gde je 50% etanolni ekstrakt, nakon
90 minuta ekstrakcije sadržao najveću količinu ukupnih polifenola, dok su najniže vrednosti
zabeležene kod 96% etanolnog ekstrakta, nakon 15 minuta (1173,21 mg GA/g suve droge,
odnosno 102,56 mg GA/g suve droge). Primenom spektrofotometrijskih metoda, DPPH metode i
ABTS metode, određena je antioksidativna aktivnost 50% i 96% etanolnih ekstrakata T.
serpyllum. Statistika je pokazala da postoji značajna razlika između antioksidativnog kapaciteta
50% i 96% etanolnih ekstrakata. Najlošiju antioksidativnu aktivnost poseduje 96% etanolni
ekstrakt, nakon 15 minuta ekstrakcije (IC50 31,323 mg/ml i 116 mM Troloxa/g suve droge).
Sadržaj ukupnih fenola i antioksidativna aktivnost ekstrakata zavise od tipa rastvarača
korišćenog za ekstrakciju i vremena ekstrakcije. Ovaj rad pokazuje da postoji pozitivna
korelacija između sadržaja ukupnih polifenola i kapaciteta neutralizacije DPPH i ABTS
radikala.",
publisher = "Faculty of Technology, Zvornik",
journal = "IV International Congress of Engineering, Environment and Materials in Processing Industry",
title = "Antioksidativna aktivnost etanolnih ekstrakata Thymus serpyllum",
pages = "452-444",
doi = "10.7251/EEMSR1501444J"
}
