TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Skrt, Mihaela
AU  - Petrović, Predrag
AU  - Janković-Častvan, Ivona
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4288
AB  - The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity
EP  - 29
IS  - 39
SP  - 23
DO  - 10.5937/leksir1939023J
ER  - 

@article{
author = "Jovanović, Aleksandra and Skrt, Mihaela and Petrović, Predrag and Janković-Častvan, Ivona and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2019",
abstract = "The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity",
pages = "29-23",
number = "39",
doi = "10.5937/leksir1939023J"
}

Jovanović, A., Skrt, M., Petrović, P., Janković-Častvan, I., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2019). Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(39), 23-29.
https://doi.org/10.5937/leksir1939023J

Jovanović A, Skrt M, Petrović P, Janković-Častvan I, Zdunić G, Šavikin K, Bugarski B. Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine. 2019;(39):23-29.
doi:10.5937/leksir1939023J .

Jovanović, Aleksandra, Skrt, Mihaela, Petrović, Predrag, Janković-Častvan, Ivona, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity" in Lekovite sirovine, no. 39 (2019):23-29,
https://doi.org/10.5937/leksir1939023J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Ota, Ajda
AU  - Skrt, Mihaela
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Nedović, Viktor
AU  - Poklar-Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4088
AB  - Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol
VL  - 183
DO  - 10.1016/j.colsurfb.2019.110422
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Ota, Ajda and Skrt, Mihaela and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša",
year = "2019",
abstract = "Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol",
volume = "183",
doi = "10.1016/j.colsurfb.2019.110422"
}

Jovanović, A., Balanč, B., Đorđević, V., Ota, A., Skrt, M., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2019). Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 183.
https://doi.org/10.1016/j.colsurfb.2019.110422

Jovanović A, Balanč B, Đorđević V, Ota A, Skrt M, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces. 2019;183.
doi:10.1016/j.colsurfb.2019.110422 .

Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Ota, Ajda, Skrt, Mihaela, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol" in Colloids and Surfaces B-Biointerfaces, 183 (2019),
https://doi.org/10.1016/j.colsurfb.2019.110422 . .