@conference{
author = "Stefanović, Andrea and Jovanović, Jelena and Gluvić, Ana and Jakovetić Tanasković, Sonja and Luković, Nevena and Žuža Praštalo, Milena and Knežević-Jugović, Zorica",
year = "2013",
abstract = "The kinetics of hydrolysis of egg white proteins by Alcalase 2.4L (protease from Bacillus licheniformis) was investigated. In this paper, the main objective was to determine the
appropriate empirical model for the proteolytic hydrolysis of egg white. A logarithmic
equation X = (1/b) ln (1 + abt) indicating the relationship between the degree of hydrolysis
(DH) and time was established. Experimental data were successfully fitted with kinetic
model taking into account the enzyme inactivation and substrate inhibition, and related
kinetic constants were determined. The kinetic study was performed at optimum process
parameters: initial enzyme concentration of Alcalase 2.4L, initial substrate concentration
of the egg white proteins and different temperatures of the reaction. The kinetic and
thermodynamic constants for reaction of hydrolysis (Ki = 320.07 mg cm-3, k2 =0.0104 min-1,
kd = 0.0743 min-1, Ea = 103.04 kJ mol-1, Ed = 99.52 kJ mol-1) were responsible for the
empirical equation. Kinetic constants of the hydrolysis were determined, and good
congruence between the model and experimental data was achieved. The results of
nonlinear regression of the proposed kinetic model agreed with the experimental data,
thus the kinetic equations can be used to fit the enzymatic hydrolysis process of egg white
protein and to optimize the operating parameters for the bioreactor design.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of abstracts / 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS",
title = "Kinetic model of the hydrolysis of egg white proteins by Alcalase",
pages = "235",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6278"
}
