TY  - JOUR
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Boguszewska, Anastazja
AU  - Kowalski, Rafał
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6713
AB  - Dandelion is a sustainable biomass that has occasional use in the food and pharmaceutical industry. However, commercial use of its extracts is restricted by the lack of systematic information on safety, composition, and biological activity. To broaden dandelion use, the present study brings new knowledge on extracts from dandelion seeds that could find application as safe and functional food ingredients. The employed two-step supercritical fluid extraction process, followed by the conventional extraction, enabled the separation of extracts with increased content of phenolics, tocopherols, and essential fatty acids. Those compounds were found to be the major components of dandelion extracts, reaching high values up to 799 mg GE/g, 249 mg α-TE/g, and 75%, respectively. The extracts also contained significant amounts of flavonoids (up to 20.5 mg QE/g) and pigments (chlorophyll A up to 1.0 g/kg, chlorophyll B up to 0.3 g/kg, and carotenoids up to 0.1 g/kg). This study revealed new bioproducts that express strong antioxidant (reaching IC50 values up to 132.5 μg/mL) and antimicrobial activity (with minimum inhibitory concentrations up to 80.0 μg/mL). Among the tested 23 microbial strains, the extracts exhibited the strongest activity against food-borne pathogens Micrococcus luteus and Bacillus subtilis. Furthermore, the antitumor and antiviral activity of separated dandelion biological products was evaluated.
PB  - Institution of Chemical Engineers
T2  - Food and Bioproducts Processing
T1  - Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity
EP  - 81
SP  - 70
VL  - 142
DO  - 10.1016/j.fbp.2023.09.005
ER  - 

@article{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Boguszewska, Anastazja and Kowalski, Rafał and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Dandelion is a sustainable biomass that has occasional use in the food and pharmaceutical industry. However, commercial use of its extracts is restricted by the lack of systematic information on safety, composition, and biological activity. To broaden dandelion use, the present study brings new knowledge on extracts from dandelion seeds that could find application as safe and functional food ingredients. The employed two-step supercritical fluid extraction process, followed by the conventional extraction, enabled the separation of extracts with increased content of phenolics, tocopherols, and essential fatty acids. Those compounds were found to be the major components of dandelion extracts, reaching high values up to 799 mg GE/g, 249 mg α-TE/g, and 75%, respectively. The extracts also contained significant amounts of flavonoids (up to 20.5 mg QE/g) and pigments (chlorophyll A up to 1.0 g/kg, chlorophyll B up to 0.3 g/kg, and carotenoids up to 0.1 g/kg). This study revealed new bioproducts that express strong antioxidant (reaching IC50 values up to 132.5 μg/mL) and antimicrobial activity (with minimum inhibitory concentrations up to 80.0 μg/mL). Among the tested 23 microbial strains, the extracts exhibited the strongest activity against food-borne pathogens Micrococcus luteus and Bacillus subtilis. Furthermore, the antitumor and antiviral activity of separated dandelion biological products was evaluated.",
publisher = "Institution of Chemical Engineers",
journal = "Food and Bioproducts Processing",
title = "Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity",
pages = "81-70",
volume = "142",
doi = "10.1016/j.fbp.2023.09.005"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Boguszewska, A., Kowalski, R., Tyśkiewicz, K.,& Konkol, M.. (2023). Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity. in Food and Bioproducts Processing
Institution of Chemical Engineers., 142, 70-81.
https://doi.org/10.1016/j.fbp.2023.09.005

Milovanović S, Grzegorczyk A, Świątek Ł, Boguszewska A, Kowalski R, Tyśkiewicz K, Konkol M. Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity. in Food and Bioproducts Processing. 2023;142:70-81.
doi:10.1016/j.fbp.2023.09.005 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Boguszewska, Anastazja, Kowalski, Rafał, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Phenolic, tocopherol, and essential fatty acid-rich extracts from dandelion seeds: Chemical composition and biological activity" in Food and Bioproducts Processing, 142 (2023):70-81,
https://doi.org/10.1016/j.fbp.2023.09.005 . .

TY  - CONF
AU  - Stamenić, Marko
AU  - Milovanović, Stoja
AU  - Kowalski, Rafał
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6908
AB  - Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.
PB  - Budapest : University of Technology and Economics
C3  - Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
T1  - Supercritical fluid extraction from dandelion seeds
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6908
ER  - 

@conference{
author = "Stamenić, Marko and Milovanović, Stoja and Kowalski, Rafał and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "Investigations of the process of supercritical fluid extraction (SFE) from dandelion were so far mainly
focused on extractions from herb, leaves and root [1-3]. In the work presented here, the extraction process
from seeds was investigated on a laboratory and semi-industrial scale equipment. The milled plant material
with an average particle size of 0.41 ± 0.02 mm was used in all experiments. All SFEs were conducted with
carbon-dioxide in supercritical state (SCCO2) as a solvent on pressure of 450 bar and temperature of 60°C, as
these parameters were previously shown to be optimal for obtaining high-value extracts from dandelion
seeds [4]. Experiments were conducted on laboratory (dia.=7.7 cm, vol=0.56 l, CO2 flow=11 kg/h) and semiindustrial
(dia.=23.5 cm, vol.=397 l, CO2 flow=240 kg/h) scale equipment. On both scales several
experiments were conducted with initial mass of the plant material (Ms) being the only variable parameter:
laboratory scale Ms=26–104 g; semi-industrial scale Ms=2.2–8.8 kg. Fractions of extract were collected
during the extractions in order to obtain data on the kinetics of the SFE processes.
The results of the performed
experiments are depicted in Figure 1.
It can be seen that for both scales the
rate of SFE, as well as the final yield,
increases with increase of the mass
of plant material, indicating that with
the selected conditions, namely
flowrate of CO2, the residence time
was not long enough to allow
saturation of the CO2 in the first part
of the process, nor to enable
extraction of all available extract.
Similar trends on both scales can be
perceived as a good basis for further
investigations aiming at scale-up of the process of SFE from dandelion seeds. Experiments with larger
packed bed height, which would enable higher residence times, should be conducted in order to achieve
saturation at least in the first part of the SFE process. Mathematical models of the SFE process, supported by
models for estimation of the kinetic parameters, should be applied to gain full insight into the
phenomenology of the SFE process.",
publisher = "Budapest : University of Technology and Economics",
journal = "Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023",
title = "Supercritical fluid extraction from dandelion seeds",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6908"
}

Stamenić, M., Milovanović, S., Kowalski, R., Tyśkiewicz, K.,& Konkol, M.. (2023). Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023
Budapest : University of Technology and Economics., 136.
https://hdl.handle.net/21.15107/rcub_technorep_6908

Stamenić M, Milovanović S, Kowalski R, Tyśkiewicz K, Konkol M. Supercritical fluid extraction from dandelion seeds. in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023. 2023;:136.
https://hdl.handle.net/21.15107/rcub_technorep_6908 .

Stamenić, Marko, Milovanović, Stoja, Kowalski, Rafał, Tyśkiewicz, Katarzyna, Konkol, Marcin, "Supercritical fluid extraction from dandelion seeds" in Book of Abstract / 19th European Meeting on Supercritical Fluids, Budapest, Hungary, 21-24 May 2023 (2023):136,
https://hdl.handle.net/21.15107/rcub_technorep_6908 .