TY  - JOUR
AU  - Pantić, Nevena
AU  - Spasojević, Milica
AU  - Stojanović, Željko
AU  - Veljović, Đorđe
AU  - Krstić, Jugoslav
AU  - Balaž, Ana Marija
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5096
AB  - Novel macroporous copolymers of glycidyl methacrylate and ethylene glycol dimethacrylate with mean pore size diameters ranging from 150 to 310 nm were synthesized by dispersion polymerization and modified with ethylenediamine. The glutaraldehyde and periodate method were employed to immobilize horseradish peroxidase (HRP) onto these carriers. The activity of the immobilized enzyme was greatly affected by the pore size of the carrier. The highest specific activities of 9.65 and 8.94 U/g of dry weight were obtained for HRP immobilized by the periodate-route onto poly(GMA‐co‐EGDMA) carriers with pore size diameters of 234 and 297 nm, respectively. Stability studies showed an improved operational stability of immobilized peroxidase at 65 °C and in an organic solvent. HRP immobilized on a copolymer with a pore size of 234 nm, showing the highest specific activity and good stability, had higher activities at almost all pH values than the native enzyme and the increased Km value for pyrogallol oxidation. Immobilized HRP retained 80% of its original activity after five consecutive cycles of the pyrogallol oxidation and 98% of its initial activity in a storage stability study. Enzyme immobilized onto the macroporous copolymer with the pore size diameter of 234 nm showed a substantial degree of phenol removal achieved by immobilized peroxidase.
PB  - Springer
T2  - Journal of Polymers and the Environment
T1  - Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol
DO  - 10.1007/s10924-021-02364-3
ER  - 

@article{
author = "Pantić, Nevena and Spasojević, Milica and Stojanović, Željko and Veljović, Đorđe and Krstić, Jugoslav and Balaž, Ana Marija and Prodanović, Radivoje and Prodanović, Olivera",
year = "2022",
abstract = "Novel macroporous copolymers of glycidyl methacrylate and ethylene glycol dimethacrylate with mean pore size diameters ranging from 150 to 310 nm were synthesized by dispersion polymerization and modified with ethylenediamine. The glutaraldehyde and periodate method were employed to immobilize horseradish peroxidase (HRP) onto these carriers. The activity of the immobilized enzyme was greatly affected by the pore size of the carrier. The highest specific activities of 9.65 and 8.94 U/g of dry weight were obtained for HRP immobilized by the periodate-route onto poly(GMA‐co‐EGDMA) carriers with pore size diameters of 234 and 297 nm, respectively. Stability studies showed an improved operational stability of immobilized peroxidase at 65 °C and in an organic solvent. HRP immobilized on a copolymer with a pore size of 234 nm, showing the highest specific activity and good stability, had higher activities at almost all pH values than the native enzyme and the increased Km value for pyrogallol oxidation. Immobilized HRP retained 80% of its original activity after five consecutive cycles of the pyrogallol oxidation and 98% of its initial activity in a storage stability study. Enzyme immobilized onto the macroporous copolymer with the pore size diameter of 234 nm showed a substantial degree of phenol removal achieved by immobilized peroxidase.",
publisher = "Springer",
journal = "Journal of Polymers and the Environment",
title = "Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol",
doi = "10.1007/s10924-021-02364-3"
}

Pantić, N., Spasojević, M., Stojanović, Ž., Veljović, Đ., Krstić, J., Balaž, A. M., Prodanović, R.,& Prodanović, O.. (2022). Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol. in Journal of Polymers and the Environment
Springer..
https://doi.org/10.1007/s10924-021-02364-3

Pantić N, Spasojević M, Stojanović Ž, Veljović Đ, Krstić J, Balaž AM, Prodanović R, Prodanović O. Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol. in Journal of Polymers and the Environment. 2022;.
doi:10.1007/s10924-021-02364-3 .

Pantić, Nevena, Spasojević, Milica, Stojanović, Željko, Veljović, Đorđe, Krstić, Jugoslav, Balaž, Ana Marija, Prodanović, Radivoje, Prodanović, Olivera, "Immobilization of Horseradish Peroxidase on Macroporous Glycidyl-Based Copolymers with Different Surface Characteristics for the Removal of Phenol" in Journal of Polymers and the Environment (2022),
https://doi.org/10.1007/s10924-021-02364-3 . .

TY  - JOUR
AU  - Stojanović, Željko
AU  - Jeremić, Katarina
AU  - Jovanović, Slobodan
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5731
AB  - The dilute solution behavior of six carboxymethyl starch (CMS) samples was investigated in aqueous NaCl solutions of different concentration (0.01, 0.05, 0.1, and 0.5 M) by dynamic light scattering (DLS) at 25°C. The values of weight average molar mass, Mw, of the investigated CMS samples were in the range from 9 × 105 to 2.1 × 107 g/mol. Three of the CMS samples were obtained from corn starch and the other three from potato starch. They had different degrees of substitution, DS, ranging from 0.4 to 1.1. The translational diffusion coefficient, Dz, and hydrodynamic radius, Rh, were determined for all CMS samples in aqueous NaCl solutions of different concentrations by the DLS method. The obtained results were treated together with previous results obtained by static light scattering and viscometry, which enabled the calculation of the structure-sensitive parameter, ρ, and the dependence of Rh, radius of gyration, Rg, and viscosity radius, Rη, on molar mass. The calculated values of the ρ parameter were independent of molar mass. The parameter ρ did not change significantly with the variation of the salt concentration in water, its values being in the range from 1.10 to 1.19. The values of exponent ni in the log-log dependence of Rg, Rh, and Rη on Mw were in the range between 0.42 and 0.47; 0.36 and 0.49; 0.42 and 0.44, respectively. These values are characteristic of branched structures of macromolecules.
PB  - Wiley-VCH Verlag
T2  - Starch/Staerke
T1  - Molecular structure of carboxymethyl starch in dilute aqueous sodium chloride solutions
EP  - 911
IS  - 11-12
SP  - 902
VL  - 65
DO  - 10.1002/star.201200225
ER  - 

@article{
author = "Stojanović, Željko and Jeremić, Katarina and Jovanović, Slobodan",
year = "2013",
abstract = "The dilute solution behavior of six carboxymethyl starch (CMS) samples was investigated in aqueous NaCl solutions of different concentration (0.01, 0.05, 0.1, and 0.5 M) by dynamic light scattering (DLS) at 25°C. The values of weight average molar mass, Mw, of the investigated CMS samples were in the range from 9 × 105 to 2.1 × 107 g/mol. Three of the CMS samples were obtained from corn starch and the other three from potato starch. They had different degrees of substitution, DS, ranging from 0.4 to 1.1. The translational diffusion coefficient, Dz, and hydrodynamic radius, Rh, were determined for all CMS samples in aqueous NaCl solutions of different concentrations by the DLS method. The obtained results were treated together with previous results obtained by static light scattering and viscometry, which enabled the calculation of the structure-sensitive parameter, ρ, and the dependence of Rh, radius of gyration, Rg, and viscosity radius, Rη, on molar mass. The calculated values of the ρ parameter were independent of molar mass. The parameter ρ did not change significantly with the variation of the salt concentration in water, its values being in the range from 1.10 to 1.19. The values of exponent ni in the log-log dependence of Rg, Rh, and Rη on Mw were in the range between 0.42 and 0.47; 0.36 and 0.49; 0.42 and 0.44, respectively. These values are characteristic of branched structures of macromolecules.",
publisher = "Wiley-VCH Verlag",
journal = "Starch/Staerke",
title = "Molecular structure of carboxymethyl starch in dilute aqueous sodium chloride solutions",
pages = "911-902",
number = "11-12",
volume = "65",
doi = "10.1002/star.201200225"
}

Stojanović, Ž., Jeremić, K.,& Jovanović, S.. (2013). Molecular structure of carboxymethyl starch in dilute aqueous sodium chloride solutions. in Starch/Staerke
Wiley-VCH Verlag., 65(11-12), 902-911.
https://doi.org/10.1002/star.201200225

Stojanović Ž, Jeremić K, Jovanović S. Molecular structure of carboxymethyl starch in dilute aqueous sodium chloride solutions. in Starch/Staerke. 2013;65(11-12):902-911.
doi:10.1002/star.201200225 .

Stojanović, Željko, Jeremić, Katarina, Jovanović, Slobodan, "Molecular structure of carboxymethyl starch in dilute aqueous sodium chloride solutions" in Starch/Staerke, 65, no. 11-12 (2013):902-911,
https://doi.org/10.1002/star.201200225 . .

TY  - JOUR
AU  - Jovanović, Slobodan
AU  - Džunuzović, Jasna
AU  - Stojanović, Željko
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5710
AB  - A short review of biopolymers based on starch (starch derivatives, thermoplastic starch), lignin and hemicelluloses, chitin (chitosan) and products obtained by degradation of starch and other polysaccharides and sugars (poly(lactic acid), poly(hydroxyalkanoates)), as well as some of their basic properties and application area, are given in this part. The problem of environmental and economic feasibility of biopolymers based on renewable raw materials and their competitiveness with polymers based on fossil raw materials is discussed. Also pointed out are the problems that appear due to the increasing use of agricultural land for the production of raw materials for the chemical industry and energy, instead for the production of food for humans and animals. The optimistic assessments of experts considering the development perspectives of biopolymers based on renewable raw materials in the next ten years have also been pointed out. At the end of the paper, the success of a team of researchers gathered around the experts from the company Bayer is indicated. They were the first in the world to develop a catalyst by which they managed to effectively activate CO- and incorporate it into polyols, used for the synthesis of polyurethanes in semi-industrial scale. By applying this process, for the first time a pollutant will be used as a basic raw material for the synthesis of organic compounds, which will have significant consequences on the development of the chemical industry, and therefore the production of polymers.
AB  - U okviru ovoga dijela rada dan je kratak prikaz biopolimera na bazi škroba (derivati škroba, termoplastièni škrob), lignina i hemiceluloze, hitina (hitozan) i na bazi proizvoda razgradnje škroba, drugih polisaharida i šećera (poli(mliječna kiselina), polihidroksialkanoati), kao i neka njihova osnovna svojstva i područje primjene. Razmatran je problem ekološke i ekonomske prihvatljivosti biopolimera na bazi obnovljivih sirovina i njihove konkurentnosti s polimerima na bazi fosilnih sirovina. Ukazano je i na probleme do kojih već dolazi zbog sve veće upotrebe poljoprivrednog zemljišta za proizvodnju sirovina za kemijsku industriju i energente, umjesto za proizvodnju hrane za ljude i životinje. Ukazano je također na optimistične procjene stručnjaka o perspektivi razvoja proizvodnje biopolimera na bazi obnovljivih sirovina. Na kraju rada je ukazano i na uspjeh tima istraživača okupljenih oko stručnjaka tvrtke Bayer, koji su prvi u svijetu razvili katalizator, pomoću kojeg su uspjeli učinkovito aktivirati CO i ugraditi ga u poliole, koje upotrebljavaju za sintezu poliuretana u poluindrustrijskim razmjerima. Ovim postupkom će prvi put jedan zagađivač biti upotrijebljen kao osnovna sirovina za sintezu organskih spojeva, što će imati nesagledive posljedice na razvoj kemijske industrije, a samim tim i proizvodnje polimera.
PB  - Croatian Society of Chemical Engineers
T2  - Kemija u industriji/Journal of Chemists and Chemical Engineers
T1  - Polymers based on renewable raw materials - Part II
T1  - Polimeri na bazi obnovljivih sirovina – II. dio
EP  - 326
IS  - 9-10
SP  - 315
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_cer_1369
ER  - 

@article{
author = "Jovanović, Slobodan and Džunuzović, Jasna and Stojanović, Željko",
year = "2013",
abstract = "A short review of biopolymers based on starch (starch derivatives, thermoplastic starch), lignin and hemicelluloses, chitin (chitosan) and products obtained by degradation of starch and other polysaccharides and sugars (poly(lactic acid), poly(hydroxyalkanoates)), as well as some of their basic properties and application area, are given in this part. The problem of environmental and economic feasibility of biopolymers based on renewable raw materials and their competitiveness with polymers based on fossil raw materials is discussed. Also pointed out are the problems that appear due to the increasing use of agricultural land for the production of raw materials for the chemical industry and energy, instead for the production of food for humans and animals. The optimistic assessments of experts considering the development perspectives of biopolymers based on renewable raw materials in the next ten years have also been pointed out. At the end of the paper, the success of a team of researchers gathered around the experts from the company Bayer is indicated. They were the first in the world to develop a catalyst by which they managed to effectively activate CO- and incorporate it into polyols, used for the synthesis of polyurethanes in semi-industrial scale. By applying this process, for the first time a pollutant will be used as a basic raw material for the synthesis of organic compounds, which will have significant consequences on the development of the chemical industry, and therefore the production of polymers., U okviru ovoga dijela rada dan je kratak prikaz biopolimera na bazi škroba (derivati škroba, termoplastièni škrob), lignina i hemiceluloze, hitina (hitozan) i na bazi proizvoda razgradnje škroba, drugih polisaharida i šećera (poli(mliječna kiselina), polihidroksialkanoati), kao i neka njihova osnovna svojstva i područje primjene. Razmatran je problem ekološke i ekonomske prihvatljivosti biopolimera na bazi obnovljivih sirovina i njihove konkurentnosti s polimerima na bazi fosilnih sirovina. Ukazano je i na probleme do kojih već dolazi zbog sve veće upotrebe poljoprivrednog zemljišta za proizvodnju sirovina za kemijsku industriju i energente, umjesto za proizvodnju hrane za ljude i životinje. Ukazano je također na optimistične procjene stručnjaka o perspektivi razvoja proizvodnje biopolimera na bazi obnovljivih sirovina. Na kraju rada je ukazano i na uspjeh tima istraživača okupljenih oko stručnjaka tvrtke Bayer, koji su prvi u svijetu razvili katalizator, pomoću kojeg su uspjeli učinkovito aktivirati CO i ugraditi ga u poliole, koje upotrebljavaju za sintezu poliuretana u poluindrustrijskim razmjerima. Ovim postupkom će prvi put jedan zagađivač biti upotrijebljen kao osnovna sirovina za sintezu organskih spojeva, što će imati nesagledive posljedice na razvoj kemijske industrije, a samim tim i proizvodnje polimera.",
publisher = "Croatian Society of Chemical Engineers",
journal = "Kemija u industriji/Journal of Chemists and Chemical Engineers",
title = "Polymers based on renewable raw materials - Part II, Polimeri na bazi obnovljivih sirovina – II. dio",
pages = "326-315",
number = "9-10",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_cer_1369"
}

Jovanović, S., Džunuzović, J.,& Stojanović, Ž.. (2013). Polymers based on renewable raw materials - Part II. in Kemija u industriji/Journal of Chemists and Chemical Engineers
Croatian Society of Chemical Engineers., 62(9-10), 315-326.
https://hdl.handle.net/21.15107/rcub_cer_1369

Jovanović S, Džunuzović J, Stojanović Ž. Polymers based on renewable raw materials - Part II. in Kemija u industriji/Journal of Chemists and Chemical Engineers. 2013;62(9-10):315-326.
https://hdl.handle.net/21.15107/rcub_cer_1369 .

Jovanović, Slobodan, Džunuzović, Jasna, Stojanović, Željko, "Polymers based on renewable raw materials - Part II" in Kemija u industriji/Journal of Chemists and Chemical Engineers, 62, no. 9-10 (2013):315-326,
https://hdl.handle.net/21.15107/rcub_cer_1369 .

TY  - JOUR
AU  - Jovanović, Slobodan
AU  - Džunuzović, Jasna
AU  - Stojanović, Željko
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5709
AB  - This paper gives an overview of the production and application of polymer materials based on renewable raw materials * biopolymers. It is pointed out that, investment of resources in the study of renewable raw materials in the last twenty years has led to the improvement of old and development of completely new chemical and biochemical processes for using biomass for the production of low molecular weight chemical substances, and especially for the production of biopolymers, which are biodegradable and compostable, and biopolymers which are nonbiodegradable. In the same period, producers of polymers based on fossil raw materials have also developed biopolymers that are biodegradable and some of them compostable and, most important, compatible with biopolymers based on renewable raw materials. The facts considering the state of biopolymers based on renewable raw materials on the market, and prediction of production increase over the next five years are also stated. Additionally, the main renewable raw materials and the biopolymers made from them that are already present in the world market are briefly listed. A short review of biopolymers based on cellulose from wood and annual plants is also given.
AB  - U okviru ovoga rada dan je pregled stanja u proizvodnji i primjeni polimernih materijala na bazi obnovljivih sirovina – biopolimera. Ukazano je na to da su ulaganjem sredstava u izučavanje obnovljivih sirovina u posljednjih dvadesetak godina unaprijeđeni stari i razvijeni potpuno novi kemijski i biokemijski postupci uporabe biomase za dobivanje niskomolekulskih kemijskih supstancija, a posebno biopolimera koji su i biorazgradivi i kompostabilni, ali i biopolimera koji su bionerazgradivi. U istom razdoblju su i proizvođači polimera na bazi fosilnih sirovina razvili biopolimere koji su biorazgradivi, a neki i kompostabilni i što je vrlo bitno kompatibilni s biopolimerima na bazi obnovljivih sirovina. Navedeni su i podaci o stanju na tržištu biopolimera na bazi obnovljivih sirovina, kao i procjene povećanja proizvodnje u sljedećih desetak godina. Pored toga ukratko su prikazane osnovne obnovljive sirovine, kao i biopolimeri koji se od njih proizvode i veze su prisutni na svjetskom tržištu. Dan je kratak prikaz biopolimera na bazi celuloze iz drveta i jednogodišnjih biljaka.
PB  - Croatian Society of Chemical Engineers
T2  - Kemija u industriji/Journal of Chemists and Chemical Engineers
T1  - Polymers based on renewable raw materials - Part I
T1  - Polimeri na bazi obnovljivih sirovina – I. dio
EP  - 314
IS  - 9-10
SP  - 307
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_cer_1380
ER  - 

@article{
author = "Jovanović, Slobodan and Džunuzović, Jasna and Stojanović, Željko",
year = "2013",
abstract = "This paper gives an overview of the production and application of polymer materials based on renewable raw materials * biopolymers. It is pointed out that, investment of resources in the study of renewable raw materials in the last twenty years has led to the improvement of old and development of completely new chemical and biochemical processes for using biomass for the production of low molecular weight chemical substances, and especially for the production of biopolymers, which are biodegradable and compostable, and biopolymers which are nonbiodegradable. In the same period, producers of polymers based on fossil raw materials have also developed biopolymers that are biodegradable and some of them compostable and, most important, compatible with biopolymers based on renewable raw materials. The facts considering the state of biopolymers based on renewable raw materials on the market, and prediction of production increase over the next five years are also stated. Additionally, the main renewable raw materials and the biopolymers made from them that are already present in the world market are briefly listed. A short review of biopolymers based on cellulose from wood and annual plants is also given., U okviru ovoga rada dan je pregled stanja u proizvodnji i primjeni polimernih materijala na bazi obnovljivih sirovina – biopolimera. Ukazano je na to da su ulaganjem sredstava u izučavanje obnovljivih sirovina u posljednjih dvadesetak godina unaprijeđeni stari i razvijeni potpuno novi kemijski i biokemijski postupci uporabe biomase za dobivanje niskomolekulskih kemijskih supstancija, a posebno biopolimera koji su i biorazgradivi i kompostabilni, ali i biopolimera koji su bionerazgradivi. U istom razdoblju su i proizvođači polimera na bazi fosilnih sirovina razvili biopolimere koji su biorazgradivi, a neki i kompostabilni i što je vrlo bitno kompatibilni s biopolimerima na bazi obnovljivih sirovina. Navedeni su i podaci o stanju na tržištu biopolimera na bazi obnovljivih sirovina, kao i procjene povećanja proizvodnje u sljedećih desetak godina. Pored toga ukratko su prikazane osnovne obnovljive sirovine, kao i biopolimeri koji se od njih proizvode i veze su prisutni na svjetskom tržištu. Dan je kratak prikaz biopolimera na bazi celuloze iz drveta i jednogodišnjih biljaka.",
publisher = "Croatian Society of Chemical Engineers",
journal = "Kemija u industriji/Journal of Chemists and Chemical Engineers",
title = "Polymers based on renewable raw materials - Part I, Polimeri na bazi obnovljivih sirovina – I. dio",
pages = "314-307",
number = "9-10",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_cer_1380"
}

Jovanović, S., Džunuzović, J.,& Stojanović, Ž.. (2013). Polymers based on renewable raw materials - Part I. in Kemija u industriji/Journal of Chemists and Chemical Engineers
Croatian Society of Chemical Engineers., 62(9-10), 307-314.
https://hdl.handle.net/21.15107/rcub_cer_1380

Jovanović S, Džunuzović J, Stojanović Ž. Polymers based on renewable raw materials - Part I. in Kemija u industriji/Journal of Chemists and Chemical Engineers. 2013;62(9-10):307-314.
https://hdl.handle.net/21.15107/rcub_cer_1380 .

Jovanović, Slobodan, Džunuzović, Jasna, Stojanović, Željko, "Polymers based on renewable raw materials - Part I" in Kemija u industriji/Journal of Chemists and Chemical Engineers, 62, no. 9-10 (2013):307-314,
https://hdl.handle.net/21.15107/rcub_cer_1380 .

TY  - CONF
AU  - Jakovetić, Sonja
AU  - Picazo-Espinosa, Rafael
AU  - Manzanera, Maximino
AU  - Stojanović, Željko
AU  - Prodanović, Radivoje
AU  - Miladinović, Ružica
AU  - Knežević-Jugović, Zorica
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096
AB  - Immobilization of lipase B from Candida antarctica was carried out using three different
immobilization methods, and two epoxy activated supports, comercial Eupergit C and
synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol
dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB
immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of
the effect of immobilization pH on the activity of immobilized enzymes, showed that the
highest activities were obtained when immobilization process was conducted in acidic
enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was
achieved when periodate method was employed. Glutaraldehyde activation of support did
not improve activity of obtained immobilized enzymes.
T1  - Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6096
ER  - 

@conference{
author = "Jakovetić, Sonja and Picazo-Espinosa, Rafael and Manzanera, Maximino and Stojanović, Željko and Prodanović, Radivoje and Miladinović, Ružica and Knežević-Jugović, Zorica",
year = "2012",
abstract = "Immobilization of lipase B from Candida antarctica was carried out using three different
immobilization methods, and two epoxy activated supports, comercial Eupergit C and
synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol
dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB
immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of
the effect of immobilization pH on the activity of immobilized enzymes, showed that the
highest activities were obtained when immobilization process was conducted in acidic
enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was
achieved when periodate method was employed. Glutaraldehyde activation of support did
not improve activity of obtained immobilized enzymes.",
title = "Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6096"
}

Jakovetić, S., Picazo-Espinosa, R., Manzanera, M., Stojanović, Ž., Prodanović, R., Miladinović, R.,& Knežević-Jugović, Z.. (2012). Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment. , 129.
https://hdl.handle.net/21.15107/rcub_technorep_6096

Jakovetić S, Picazo-Espinosa R, Manzanera M, Stojanović Ž, Prodanović R, Miladinović R, Knežević-Jugović Z. Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment. 2012;:129.
https://hdl.handle.net/21.15107/rcub_technorep_6096 .

Jakovetić, Sonja, Picazo-Espinosa, Rafael, Manzanera, Maximino, Stojanović, Željko, Prodanović, Radivoje, Miladinović, Ružica, Knežević-Jugović, Zorica, "Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment" (2012):129,
https://hdl.handle.net/21.15107/rcub_technorep_6096 .

TY  - JOUR
AU  - Prodanović, Olivera
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Knežević-Jugović, Zorica
AU  - Prodanović, Radivoje
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2187
AB  - A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.
PB  - Springer, New York
T2  - Applied Biochemistry and Biotechnology
T1  - Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers
EP  - 1301
IS  - 5
SP  - 1288
VL  - 168
DO  - 10.1007/s12010-012-9857-7
ER  - 

@article{
author = "Prodanović, Olivera and Prokopijević, Miloš and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Knežević-Jugović, Zorica and Prodanović, Radivoje",
year = "2012",
abstract = "A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers",
pages = "1301-1288",
number = "5",
volume = "168",
doi = "10.1007/s12010-012-9857-7"
}

Prodanović, O., Prokopijević, M., Spasojević, D., Stojanović, Ž., Radotić, K., Knežević-Jugović, Z.,& Prodanović, R.. (2012). Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers. in Applied Biochemistry and Biotechnology
Springer, New York., 168(5), 1288-1301.
https://doi.org/10.1007/s12010-012-9857-7

Prodanović O, Prokopijević M, Spasojević D, Stojanović Ž, Radotić K, Knežević-Jugović Z, Prodanović R. Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers. in Applied Biochemistry and Biotechnology. 2012;168(5):1288-1301.
doi:10.1007/s12010-012-9857-7 .

Prodanović, Olivera, Prokopijević, Miloš, Spasojević, Dragica, Stojanović, Željko, Radotić, Ksenija, Knežević-Jugović, Zorica, Prodanović, Radivoje, "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers" in Applied Biochemistry and Biotechnology, 168, no. 5 (2012):1288-1301,
https://doi.org/10.1007/s12010-012-9857-7 . .

TY  - JOUR
AU  - Stojanović, Željko
AU  - Jeremić, Katarina
AU  - Jovanović, Slobodan
AU  - Nierling, Wolfgang
AU  - Lechner, Manfred Dieter
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5622
AB  - Uzorci karboksimetil skroba pripremljeni su reakcijom eterifikacije u heterogenom
medijumu. Tri uzorka su pripremljena iz kukuruznog skroba a tri iz skroba iz krompira. Stepen supstitucije sintetisanih uzoraka je iznosio od 0,40 do 1,10. Uzorci su
ispitivani metodama statičkog i dinamičkog rasipanja svetlosti i viskozimetrijom razblaženih rastvora u 0,10 M rastvoru NaCl kao rastvaraču na 25 °C. Određene su
vrednosti molarnih masa, MW, poluprečnika rotacije, Rg, hidrodinamičkog poluprečnika, Rh, i graničnog viskozitetnog broja, [η]. Utvrđeno je da su uzorci bez obzira na
tip skroba i stepen supstitucije međusobno slični. Fraktalne dimenzije, df, iznose 2,380
(Rg – MW) i 2,406 (Rη – MW), dok parametar ρ iznosi 1,195. Vrednost fraktalne dimenzije i parametra ρ karakteristične su vrednosti za ponašanje razgranatih klastera u
termodinamički dobrim rastvaračima.
AB  - Carboxymethyl starch samples were prepared by etherification in heterogeneous media. Three samples were prepared from corn starch and three samples from potato starch. Degree of substitution of prepared samples ranged from 0.40 to 1.10. The samples were investigated by static and dynamic light scattering and viscosity of diluted solution in 0.10 M NaCl as solvent at 25 °C. The values of molar mass, MW, radius of gyration, Rg, hydrodynamic radius, Rh, and limited viscosity number, [η] were determined. It was found that the samples are similar to each other regardless of the type of starch and the degree of substitution. Fractal dimension, d f, was equal to 2.380 (Rg - MW) and 2.406 (Rη - MW), while parameter ρ was 1.195. The values of fractal dimension and parameter ρ are characteristic values for the behavior of branched clusters in thermodynamically good solvents.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Oblik i veličina makromolekula karboksimetil skroba u razblaženim rastvorima
T1  - The shape and size of macromolecules of carboxymethyl starch in diluted solutions
EP  - 602
IS  - 6
SP  - 593
VL  - 63
DO  - 10.2298/HEMIND0906593S
ER  - 

@article{
author = "Stojanović, Željko and Jeremić, Katarina and Jovanović, Slobodan and Nierling, Wolfgang and Lechner, Manfred Dieter",
year = "2009",
abstract = "Uzorci karboksimetil skroba pripremljeni su reakcijom eterifikacije u heterogenom
medijumu. Tri uzorka su pripremljena iz kukuruznog skroba a tri iz skroba iz krompira. Stepen supstitucije sintetisanih uzoraka je iznosio od 0,40 do 1,10. Uzorci su
ispitivani metodama statičkog i dinamičkog rasipanja svetlosti i viskozimetrijom razblaženih rastvora u 0,10 M rastvoru NaCl kao rastvaraču na 25 °C. Određene su
vrednosti molarnih masa, MW, poluprečnika rotacije, Rg, hidrodinamičkog poluprečnika, Rh, i graničnog viskozitetnog broja, [η]. Utvrđeno je da su uzorci bez obzira na
tip skroba i stepen supstitucije međusobno slični. Fraktalne dimenzije, df, iznose 2,380
(Rg – MW) i 2,406 (Rη – MW), dok parametar ρ iznosi 1,195. Vrednost fraktalne dimenzije i parametra ρ karakteristične su vrednosti za ponašanje razgranatih klastera u
termodinamički dobrim rastvaračima., Carboxymethyl starch samples were prepared by etherification in heterogeneous media. Three samples were prepared from corn starch and three samples from potato starch. Degree of substitution of prepared samples ranged from 0.40 to 1.10. The samples were investigated by static and dynamic light scattering and viscosity of diluted solution in 0.10 M NaCl as solvent at 25 °C. The values of molar mass, MW, radius of gyration, Rg, hydrodynamic radius, Rh, and limited viscosity number, [η] were determined. It was found that the samples are similar to each other regardless of the type of starch and the degree of substitution. Fractal dimension, d f, was equal to 2.380 (Rg - MW) and 2.406 (Rη - MW), while parameter ρ was 1.195. The values of fractal dimension and parameter ρ are characteristic values for the behavior of branched clusters in thermodynamically good solvents.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Oblik i veličina makromolekula karboksimetil skroba u razblaženim rastvorima, The shape and size of macromolecules of carboxymethyl starch in diluted solutions",
pages = "602-593",
number = "6",
volume = "63",
doi = "10.2298/HEMIND0906593S"
}

Stojanović, Ž., Jeremić, K., Jovanović, S., Nierling, W.,& Lechner, M. D.. (2009). Oblik i veličina makromolekula karboksimetil skroba u razblaženim rastvorima. in Hemijska industrija
Association of Chemical Engineers of Serbia., 63(6), 593-602.
https://doi.org/10.2298/HEMIND0906593S

Stojanović Ž, Jeremić K, Jovanović S, Nierling W, Lechner MD. Oblik i veličina makromolekula karboksimetil skroba u razblaženim rastvorima. in Hemijska industrija. 2009;63(6):593-602.
doi:10.2298/HEMIND0906593S .

Stojanović, Željko, Jeremić, Katarina, Jovanović, Slobodan, Nierling, Wolfgang, Lechner, Manfred Dieter, "Oblik i veličina makromolekula karboksimetil skroba u razblaženim rastvorima" in Hemijska industrija, 63, no. 6 (2009):593-602,
https://doi.org/10.2298/HEMIND0906593S . .