TY  - CONF
AU  - Mojović, Ljiljana
AU  - Jović, Jelena
AU  - Mladenović, Dragana
AU  - Đukić-Vuković, Aleksandra
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6245
AB  - Lignocellulosic biomass has become one of the most explored renewable substrates for the production of many valuable chemicals, biofuels, and food or feed ingredients or products. However, a complex structure blocs its direct utilization and directs to utilization of particular pretreatments in order to remove lignin and facilitate the biomass hydrolysis prior to its further processing. The pretreatments could be done by chemical, physical, physicochemical means, or using biological methods. Biological methods involving the use of fungi or their enzymes in the pretreatment and hydrolysis of the lignocellulosic substrate are ecologically friendly, rather time consuming, but with no formation of harmless components that can inhibit the production microorganism (Nair and Sivakumar, 2020). The limitations, such as process longevity and loss of valuable sugars, can be alleviated or overcome by utilization of novel efficient microorganisms, e.g. fungi which are selective biomass decomposers, as well as by optimizing conditions of the pretreatment. In this study, a potential use of novel isolated Serbian autochthonous fungi in the pretreatment of lignocellulosic biomass was studied. Isolates were collected in the southern Serbia near the City of Leskovac. The strains were isolated from stumps, fallen trees and branches, fallen leaves, or living trees found in the oak forest and orchards around the forest. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of waste lignocellulosic biomass (beechwood sawdust). Table 1. shows biomass and lignin reduction and selectivity coefficients relative to the total lignin reduction (selectivity coefficient 1), and relative to the Klason’s lignin reduction (selectivity coefficient 2) after 35 days of incubation under non optimized conditions. More lignin was degraded by T. hirsuta F13 than S. gausapatum F28: its selectivity coefficient, relative to the total lignin, was 1.7, while the selectivity coefficient obtained for S. gausapatum was 1.1. The values of the selectivity coefficient, relative to Klason's lignin, were 1.47 for the isolate F13 and 0.8 for the isolate F28, which confirmed that T. hirsuta F13 was the best candidate among the isolated fungi for use for the biomass pretreatment. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and was selected as a potential producer of important industrial hydrolytic enzymes. The three novel fungal isolates with pronounced lignocellulolytic activities were identified using ITS sequences, and the sequences were deposited in the NCBI GenBank database. Their accession numbers are KY264754.1 (Trametes hirsuta F13), KY264753.1 (Stereum gausapatum F28), and MF521930.1 (Myrmaecium fulvopruinatum F28) (Jović et al., 2018). The isolate T. hirsuta F13 which was selected as the best candidate for the pretreatment of lignocelulosic biomass was further studied in order to enhance its biomass degrading efficiency by optimizing the pretreatment conditions. It was found that the supplementation with sugar beet molasses stillage (MS) could significantly improve the biological pretreatment. The initial results showed that the addition of MS could improve ligninolytic activity, but also that the type of a dominant enzyme activity differed depending on the stillage concentration. Besides, other pretreatment parameters such as substrate moisture and temperature of the treatment were found important. The results have shown that the use of MS at the concentration of 13% as a supplement at the temperature of 25 0C and substrate moisture of 63% can shorten the time of the biological Monitored parameter Fungal isolate T. hirshuta F13 S.gauspatum F28 Biomass reduction (%) 19 24 Total lignin reduction (%) 33.8 28 Selectivity coefficient 1 1.7 1.1 Klason's lignin reduction (%) 28 19 Selectivity coefficient 2 1.47 0.8 pretreatment from 35 to 18 days, while the selectivity of biomass degradation was also improved of (Jović et al., 2020). Finally, this study examined and selected three novel autochthones fungal isolates, T. hirsuta F13, M. fulvopruinatum F14, and S. gausapatum F28, which can be used in the production of industrially important lignocellulolytic enzymes and/or in biomass pretreatment under determined conditions.
PB  - NTUA
C3  - 9th International Conference on Sustainable Solid Waste Management
T1  - Novel autochthonous fungi for the treatment of lignocellulosic biomass
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6245
ER  - 

@conference{
author = "Mojović, Ljiljana and Jović, Jelena and Mladenović, Dragana and Đukić-Vuković, Aleksandra",
year = "2022",
abstract = "Lignocellulosic biomass has become one of the most explored renewable substrates for the production of many valuable chemicals, biofuels, and food or feed ingredients or products. However, a complex structure blocs its direct utilization and directs to utilization of particular pretreatments in order to remove lignin and facilitate the biomass hydrolysis prior to its further processing. The pretreatments could be done by chemical, physical, physicochemical means, or using biological methods. Biological methods involving the use of fungi or their enzymes in the pretreatment and hydrolysis of the lignocellulosic substrate are ecologically friendly, rather time consuming, but with no formation of harmless components that can inhibit the production microorganism (Nair and Sivakumar, 2020). The limitations, such as process longevity and loss of valuable sugars, can be alleviated or overcome by utilization of novel efficient microorganisms, e.g. fungi which are selective biomass decomposers, as well as by optimizing conditions of the pretreatment. In this study, a potential use of novel isolated Serbian autochthonous fungi in the pretreatment of lignocellulosic biomass was studied. Isolates were collected in the southern Serbia near the City of Leskovac. The strains were isolated from stumps, fallen trees and branches, fallen leaves, or living trees found in the oak forest and orchards around the forest. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of waste lignocellulosic biomass (beechwood sawdust). Table 1. shows biomass and lignin reduction and selectivity coefficients relative to the total lignin reduction (selectivity coefficient 1), and relative to the Klason’s lignin reduction (selectivity coefficient 2) after 35 days of incubation under non optimized conditions. More lignin was degraded by T. hirsuta F13 than S. gausapatum F28: its selectivity coefficient, relative to the total lignin, was 1.7, while the selectivity coefficient obtained for S. gausapatum was 1.1. The values of the selectivity coefficient, relative to Klason's lignin, were 1.47 for the isolate F13 and 0.8 for the isolate F28, which confirmed that T. hirsuta F13 was the best candidate among the isolated fungi for use for the biomass pretreatment. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and was selected as a potential producer of important industrial hydrolytic enzymes. The three novel fungal isolates with pronounced lignocellulolytic activities were identified using ITS sequences, and the sequences were deposited in the NCBI GenBank database. Their accession numbers are KY264754.1 (Trametes hirsuta F13), KY264753.1 (Stereum gausapatum F28), and MF521930.1 (Myrmaecium fulvopruinatum F28) (Jović et al., 2018). The isolate T. hirsuta F13 which was selected as the best candidate for the pretreatment of lignocelulosic biomass was further studied in order to enhance its biomass degrading efficiency by optimizing the pretreatment conditions. It was found that the supplementation with sugar beet molasses stillage (MS) could significantly improve the biological pretreatment. The initial results showed that the addition of MS could improve ligninolytic activity, but also that the type of a dominant enzyme activity differed depending on the stillage concentration. Besides, other pretreatment parameters such as substrate moisture and temperature of the treatment were found important. The results have shown that the use of MS at the concentration of 13% as a supplement at the temperature of 25 0C and substrate moisture of 63% can shorten the time of the biological Monitored parameter Fungal isolate T. hirshuta F13 S.gauspatum F28 Biomass reduction (%) 19 24 Total lignin reduction (%) 33.8 28 Selectivity coefficient 1 1.7 1.1 Klason's lignin reduction (%) 28 19 Selectivity coefficient 2 1.47 0.8 pretreatment from 35 to 18 days, while the selectivity of biomass degradation was also improved of (Jović et al., 2020). Finally, this study examined and selected three novel autochthones fungal isolates, T. hirsuta F13, M. fulvopruinatum F14, and S. gausapatum F28, which can be used in the production of industrially important lignocellulolytic enzymes and/or in biomass pretreatment under determined conditions.",
publisher = "NTUA",
journal = "9th International Conference on Sustainable Solid Waste Management",
title = "Novel autochthonous fungi for the treatment of lignocellulosic biomass",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6245"
}

Mojović, L., Jović, J., Mladenović, D.,& Đukić-Vuković, A.. (2022). Novel autochthonous fungi for the treatment of lignocellulosic biomass. in 9th International Conference on Sustainable Solid Waste Management
NTUA..
https://hdl.handle.net/21.15107/rcub_technorep_6245

Mojović L, Jović J, Mladenović D, Đukić-Vuković A. Novel autochthonous fungi for the treatment of lignocellulosic biomass. in 9th International Conference on Sustainable Solid Waste Management. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6245 .

Mojović, Ljiljana, Jović, Jelena, Mladenović, Dragana, Đukić-Vuković, Aleksandra, "Novel autochthonous fungi for the treatment of lignocellulosic biomass" in 9th International Conference on Sustainable Solid Waste Management (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6245 .

TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5182
AB  - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
EP  - 450
IS  - 4
SP  - 437
VL  - 87
DO  - 10.2298/JSC200601075J
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Mojović, Ljiljana",
year = "2022",
abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage",
pages = "450-437",
number = "4",
volume = "87",
doi = "10.2298/JSC200601075J"
}

Jović, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(4), 437-450.
https://doi.org/10.2298/JSC200601075J

Jović J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;87(4):437-450.
doi:10.2298/JSC200601075J .

Jović, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society, 87, no. 4 (2022):437-450,
https://doi.org/10.2298/JSC200601075J . .

TY  - JOUR
AU  - Ćorac, Aleksandar
AU  - Barać, Nemanja
AU  - Bukumirić, Zoran
AU  - Barać, Milan
AU  - Milićević, Saša
AU  - Vidović, Milka
AU  - Mijović, Milica
AU  - Hudomal, Snežana
AU  - Joksimović, Viktorija
AU  - Paut-Kusturica, Milica
AU  - Ilić, Danijela
AU  - Jović, Jelena
AU  - Trajković, Goran
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3512
AB  - Background/Aim. The northern part of Kosovo was one of the largest lead and zinc production industries in Europe. Special attention has been paid to the landfill sites of these metals remained after past industrial activities. The inhabitants of Roma camps in this area are collecting led waste they process by crushing and melting in their shacks in primitively organized working environments. Because of all the aforementioned it was necessary to examine the concentration of blood lead level (BLL) in the children aged less than 6 years inhabiting this area, especially taking care of blood analysis of children living in Roma camps. Methods. The study was conducted in the municipality of Leposavić, Province Kosovo and Metohija, Serbia. Totally 78 subjects participated in the study. All the subjects were divided into two groups: the group I consisting of 42 children who lived in the Romas camp, and the group II with 36 children from a city kindergarten. Based on the mathematical model WRPLOT we found out that both groups of patients were in the low risk zone for industrial contamination exposure. Blood analysis was done according to the protocol provided by ESA Lead Care. Results. The average age of participants in the study was 4.60 ± 1.63 years. The mean BBL in the children from the group 1 was 19.11 µg/dL and from the group 2 4.87 µg/dL. There was a statistically significant difference in the mean values of BBL between the groups (U = 39, p  lt  0.001). All of the children from the group 1 had BBL greater than 5 µg/dL in comparison to 38.9% of the children from the group 2 (χ2 = 35.75, p  lt  0.001). Conclusion. Although both groups were located outside the zone of direct spread of pollution, the results indicate high concentrations of lead in blood of all the examined children. The concentration was higher in the children who lived in the area in which illegal processing of lead waste took place.
AB  - Uvod/Cilj. Na području severnog Kosova nalazila se jedna od najvećih industrija za proizvodnju olova i cinka u Evropi. Poseban akcenat se stavlja na deponije zaostale nakon ove industrijske proizvodnje. Na ovom području stanovnici u romskim kampovima bave se prikupljanjem olovnog otpada koji prerađuju - drobe i tope, u svojim barakama u primitivno organizovanim radnim sredinama. Zbog svega navedenog, bilo je neophodno ispitati koncentraciju olova u krvi dece mlađe od šest godina koja žive u ovom području, sa posebnim akcentom na analizu krvi dece koja žive u romskim kampovima. Metode. Naše istraživanje sprovedeno je na području opštine Leposavić, Kosovo i Metohija, Srbija, uključujući 78 ispitanika podeljenih u dve grupe: grupa I od 42 dece iz romskog kampa, i grupa II od 36 dece iz gradskog vrtića. Na osnovu matematičkog modela WRPLOT dobili smo podatak da se obe grupe ispitanika nalaze u zoni niskog rizika od izloženosti industrijskom zagađenju. Krv za analizu uzimali smo iz prsta dece. Analiza krvi vršena je prema protokolu predviđenom od ESA Biosciences Lead Care. Rezultati. Prosečna starost ispitanika iznosila je 4,60 ± 1,63 godine. Prosečna koncentracija olova u krvi u grupi I bila je 19,11 µg/dL, a u grupi II 4.87 µg/dL. Postoji statistički visokoznačajna razlika u pogledu koncentracije olova u krvi između ispitivanih grupa (U = 39; p  lt  0,001). Sva deca (100%) iz grupe I imali su koncentracije olova u krvi veće od 5 µg/dL, a iz grupe II (χ2 = 35,75; p  lt  0,001) njih 38,9%. Zaključak. Iako su obe grupe bile locirane van zone direktnog širenja zagađenja, rezultati ukazuju na visoke koncentracije olova u krvi sve ispitivane dece. Koncentracija je veća kod dece koja žive u sredini u kojoj se odvija ilegalna prerada olovnog otpada.
PB  - Military Health Department, Ministry of Defance, Serbia
T2  - Vojnosanitetski pregled
T1  - The effect of illegal lead processing on blood lead levels in children living in the mining area
T1  - Uticaj nezakonite prerade olova na nivo olova u krvi dece u rudarskoj zoni
EP  - 1024
IS  - 11
SP  - 1019
VL  - 74
DO  - 10.2298/VSP150827217C
ER  - 

@article{
author = "Ćorac, Aleksandar and Barać, Nemanja and Bukumirić, Zoran and Barać, Milan and Milićević, Saša and Vidović, Milka and Mijović, Milica and Hudomal, Snežana and Joksimović, Viktorija and Paut-Kusturica, Milica and Ilić, Danijela and Jović, Jelena and Trajković, Goran",
year = "2017",
abstract = "Background/Aim. The northern part of Kosovo was one of the largest lead and zinc production industries in Europe. Special attention has been paid to the landfill sites of these metals remained after past industrial activities. The inhabitants of Roma camps in this area are collecting led waste they process by crushing and melting in their shacks in primitively organized working environments. Because of all the aforementioned it was necessary to examine the concentration of blood lead level (BLL) in the children aged less than 6 years inhabiting this area, especially taking care of blood analysis of children living in Roma camps. Methods. The study was conducted in the municipality of Leposavić, Province Kosovo and Metohija, Serbia. Totally 78 subjects participated in the study. All the subjects were divided into two groups: the group I consisting of 42 children who lived in the Romas camp, and the group II with 36 children from a city kindergarten. Based on the mathematical model WRPLOT we found out that both groups of patients were in the low risk zone for industrial contamination exposure. Blood analysis was done according to the protocol provided by ESA Lead Care. Results. The average age of participants in the study was 4.60 ± 1.63 years. The mean BBL in the children from the group 1 was 19.11 µg/dL and from the group 2 4.87 µg/dL. There was a statistically significant difference in the mean values of BBL between the groups (U = 39, p  lt  0.001). All of the children from the group 1 had BBL greater than 5 µg/dL in comparison to 38.9% of the children from the group 2 (χ2 = 35.75, p  lt  0.001). Conclusion. Although both groups were located outside the zone of direct spread of pollution, the results indicate high concentrations of lead in blood of all the examined children. The concentration was higher in the children who lived in the area in which illegal processing of lead waste took place., Uvod/Cilj. Na području severnog Kosova nalazila se jedna od najvećih industrija za proizvodnju olova i cinka u Evropi. Poseban akcenat se stavlja na deponije zaostale nakon ove industrijske proizvodnje. Na ovom području stanovnici u romskim kampovima bave se prikupljanjem olovnog otpada koji prerađuju - drobe i tope, u svojim barakama u primitivno organizovanim radnim sredinama. Zbog svega navedenog, bilo je neophodno ispitati koncentraciju olova u krvi dece mlađe od šest godina koja žive u ovom području, sa posebnim akcentom na analizu krvi dece koja žive u romskim kampovima. Metode. Naše istraživanje sprovedeno je na području opštine Leposavić, Kosovo i Metohija, Srbija, uključujući 78 ispitanika podeljenih u dve grupe: grupa I od 42 dece iz romskog kampa, i grupa II od 36 dece iz gradskog vrtića. Na osnovu matematičkog modela WRPLOT dobili smo podatak da se obe grupe ispitanika nalaze u zoni niskog rizika od izloženosti industrijskom zagađenju. Krv za analizu uzimali smo iz prsta dece. Analiza krvi vršena je prema protokolu predviđenom od ESA Biosciences Lead Care. Rezultati. Prosečna starost ispitanika iznosila je 4,60 ± 1,63 godine. Prosečna koncentracija olova u krvi u grupi I bila je 19,11 µg/dL, a u grupi II 4.87 µg/dL. Postoji statistički visokoznačajna razlika u pogledu koncentracije olova u krvi između ispitivanih grupa (U = 39; p  lt  0,001). Sva deca (100%) iz grupe I imali su koncentracije olova u krvi veće od 5 µg/dL, a iz grupe II (χ2 = 35,75; p  lt  0,001) njih 38,9%. Zaključak. Iako su obe grupe bile locirane van zone direktnog širenja zagađenja, rezultati ukazuju na visoke koncentracije olova u krvi sve ispitivane dece. Koncentracija je veća kod dece koja žive u sredini u kojoj se odvija ilegalna prerada olovnog otpada.",
publisher = "Military Health Department, Ministry of Defance, Serbia",
journal = "Vojnosanitetski pregled",
title = "The effect of illegal lead processing on blood lead levels in children living in the mining area, Uticaj nezakonite prerade olova na nivo olova u krvi dece u rudarskoj zoni",
pages = "1024-1019",
number = "11",
volume = "74",
doi = "10.2298/VSP150827217C"
}

Ćorac, A., Barać, N., Bukumirić, Z., Barać, M., Milićević, S., Vidović, M., Mijović, M., Hudomal, S., Joksimović, V., Paut-Kusturica, M., Ilić, D., Jović, J.,& Trajković, G.. (2017). The effect of illegal lead processing on blood lead levels in children living in the mining area. in Vojnosanitetski pregled
Military Health Department, Ministry of Defance, Serbia., 74(11), 1019-1024.
https://doi.org/10.2298/VSP150827217C

Ćorac A, Barać N, Bukumirić Z, Barać M, Milićević S, Vidović M, Mijović M, Hudomal S, Joksimović V, Paut-Kusturica M, Ilić D, Jović J, Trajković G. The effect of illegal lead processing on blood lead levels in children living in the mining area. in Vojnosanitetski pregled. 2017;74(11):1019-1024.
doi:10.2298/VSP150827217C .

Ćorac, Aleksandar, Barać, Nemanja, Bukumirić, Zoran, Barać, Milan, Milićević, Saša, Vidović, Milka, Mijović, Milica, Hudomal, Snežana, Joksimović, Viktorija, Paut-Kusturica, Milica, Ilić, Danijela, Jović, Jelena, Trajković, Goran, "The effect of illegal lead processing on blood lead levels in children living in the mining area" in Vojnosanitetski pregled, 74, no. 11 (2017):1019-1024,
https://doi.org/10.2298/VSP150827217C . .