TY  - JOUR
AU  - Wang, Qinghui
AU  - Gu, Jinjie
AU  - Shu, Lin
AU  - Jiang, Weiyan
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Shi, Jiping
AU  - Baganz, Frank
AU  - Lye, Gary J.
AU  - Xiang, Wensheng
AU  - Hao, Jian
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5109
AB  - Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.
PB  - Springer Science and Business Media B.V.
T2  - World Journal of Microbiology and Biotechnology
T1  - Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production
IS  - 5
SP  - 81
VL  - 38
DO  - 10.1007/s11274-022-03266-9
ER  - 

@article{
author = "Wang, Qinghui and Gu, Jinjie and Shu, Lin and Jiang, Weiyan and Mojović, Ljiljana and Knežević-Jugović, Zorica and Shi, Jiping and Baganz, Frank and Lye, Gary J. and Xiang, Wensheng and Hao, Jian",
year = "2022",
abstract = "Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.",
publisher = "Springer Science and Business Media B.V.",
journal = "World Journal of Microbiology and Biotechnology",
title = "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production",
number = "5",
pages = "81",
volume = "38",
doi = "10.1007/s11274-022-03266-9"
}

Wang, Q., Gu, J., Shu, L., Jiang, W., Mojović, L., Knežević-Jugović, Z., Shi, J., Baganz, F., Lye, G. J., Xiang, W.,& Hao, J.. (2022). Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media B.V.., 38(5), 81.
https://doi.org/10.1007/s11274-022-03266-9

Wang Q, Gu J, Shu L, Jiang W, Mojović L, Knežević-Jugović Z, Shi J, Baganz F, Lye GJ, Xiang W, Hao J. Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology. 2022;38(5):81.
doi:10.1007/s11274-022-03266-9 .

Wang, Qinghui, Gu, Jinjie, Shu, Lin, Jiang, Weiyan, Mojović, Ljiljana, Knežević-Jugović, Zorica, Shi, Jiping, Baganz, Frank, Lye, Gary J., Xiang, Wensheng, Hao, Jian, "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production" in World Journal of Microbiology and Biotechnology, 38, no. 5 (2022):81,
https://doi.org/10.1007/s11274-022-03266-9 . .

TY  - JOUR
AU  - Lu, Xiyang
AU  - Yao, Yao
AU  - Yang, Yang
AU  - Zhang, Zhongxi
AU  - Gu, Jinjie
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Baganz, Frank
AU  - Lye, Gary
AU  - Shi, Jiping
AU  - Hao, Jian
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/14
AB  - Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Ethylene glycol and glycolic acid production by wild-typeEscherichia coli
EP  - 755
IS  - 4
SP  - 744
SP  - 755
VL  - 68
DO  - 10.1002/bab.1987
ER  - 

@article{
author = "Lu, Xiyang and Yao, Yao and Yang, Yang and Zhang, Zhongxi and Gu, Jinjie and Mojović, Ljiljana and Knežević-Jugović, Zorica and Baganz, Frank and Lye, Gary and Shi, Jiping and Hao, Jian",
year = "2021",
abstract = "Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli",
pages = "755-744-755",
number = "4",
volume = "68",
doi = "10.1002/bab.1987"
}

Lu, X., Yao, Y., Yang, Y., Zhang, Z., Gu, J., Mojović, L., Knežević-Jugović, Z., Baganz, F., Lye, G., Shi, J.,& Hao, J.. (2021). Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 68(4), 744-755.
https://doi.org/10.1002/bab.1987

Lu X, Yao Y, Yang Y, Zhang Z, Gu J, Mojović L, Knežević-Jugović Z, Baganz F, Lye G, Shi J, Hao J. Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry. 2021;68(4):744-755.
doi:10.1002/bab.1987 .

Lu, Xiyang, Yao, Yao, Yang, Yang, Zhang, Zhongxi, Gu, Jinjie, Mojović, Ljiljana, Knežević-Jugović, Zorica, Baganz, Frank, Lye, Gary, Shi, Jiping, Hao, Jian, "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli" in Biotechnology and Applied Biochemistry, 68, no. 4 (2021):744-755,
https://doi.org/10.1002/bab.1987 . .