TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Kocić-Tanackov, Sunčica
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/12
AB  - Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.
PB  - Springer Heidelberg, Heidelberg
T2  - Biomass Conversion and Biorefinery
T1  - Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation
EP  - 2765
IS  - 7
SP  - 2749
VL  - 12
DO  - 10.1007/s13399-020-00929-1
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Kocić-Tanackov, Sunčica and Mojović, Ljiljana",
year = "2022",
abstract = "Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Biomass Conversion and Biorefinery",
title = "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation",
pages = "2765-2749",
number = "7",
volume = "12",
doi = "10.1007/s13399-020-00929-1"
}

Jović, J., Hao, J., Kocić-Tanackov, S.,& Mojović, L.. (2022). Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery
Springer Heidelberg, Heidelberg., 12(7), 2749-2765.
https://doi.org/10.1007/s13399-020-00929-1

Jović J, Hao J, Kocić-Tanackov S, Mojović L. Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery. 2022;12(7):2749-2765.
doi:10.1007/s13399-020-00929-1 .

Jović, Jelena, Hao, Jian, Kocić-Tanackov, Sunčica, Mojović, Ljiljana, "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation" in Biomass Conversion and Biorefinery, 12, no. 7 (2022):2749-2765,
https://doi.org/10.1007/s13399-020-00929-1 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Milutinović, Milica
AU  - Mihajlovski, Katarina
AU  - Jović, Jelena M.
AU  - Nastasijević, Branislav J.
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3948
AB  - In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment
EP  - 78
SP  - 71
VL  - 120
DO  - 10.1016/j.micpath.2018.04.056
ER  - 

@article{
author = "Radovanović, Neda and Milutinović, Milica and Mihajlovski, Katarina and Jović, Jelena M. and Nastasijević, Branislav J. and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment",
pages = "78-71",
volume = "120",
doi = "10.1016/j.micpath.2018.04.056"
}

Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J. M., Nastasijević, B. J., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2018). Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 71-78.
https://doi.org/10.1016/j.micpath.2018.04.056

Radovanović N, Milutinović M, Mihajlovski K, Jović JM, Nastasijević BJ, Rajilić-Stojanović M, Dimitrijević-Branković S. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis. 2018;120:71-78.
doi:10.1016/j.micpath.2018.04.056 .

Radovanović, Neda, Milutinović, Milica, Mihajlovski, Katarina, Jović, Jelena M., Nastasijević, Branislav J., Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment" in Microbial Pathogenesis, 120 (2018):71-78,
https://doi.org/10.1016/j.micpath.2018.04.056 . .

TY  - JOUR
AU  - Jović, Jelena
AU  - Buntić, Aneta
AU  - Radovanović, Neda
AU  - Petrović, Bojan
AU  - Mojović, Ljiljana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3932
AB  - The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84 % with T. hirsuta F13 and 28.8 % with S. gausapatum F28.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28
EP  - 365
IS  - 3
SP  - 354
VL  - 56
DO  - 10.17113/ftb.56.03.18.5348
ER  - 

@article{
author = "Jović, Jelena and Buntić, Aneta and Radovanović, Neda and Petrović, Bojan and Mojović, Ljiljana",
year = "2018",
abstract = "The aim of this research is to isolate and identify fungi with high lignin-degrading abilities that are autochthonous to southern Serbian region. Two novel fungal isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 were selected to assess their ligninolytic enzyme activities and the efficiency of lignin removal from beech wood sawdust. Obtained results show that both isolates are good sources of industrially valuable enzymes with a potential for application in various biotechnological and industrial processes. Both isolates showed laccase, manganese-dependent peroxidase, and versatile peroxidase activities, while only S. gausapatum F28 had lignin peroxidase activity. This is the first record of the ability of S. gausapatum species to produce lignin peroxidase. T. hirsuta F13 showed higher laccase activity than S. gausapatum F28, while S. gausapatum F28 had higher manganese peroxidase activity. Also, T hirsuta F13 exhibited much higher laccase activity under submerged cultivation conditions than solid-state cultivation conditions, which is rare for fungi. This is important for industrial processes since the submerged fermentation is a dominant technique in industry. The test of the efficiency of lignin removal showed that both isolates are efficient lignin decomposers. After five weeks of incubation on beech wood sawdust, the total lignin losses were 33.84 % with T. hirsuta F13 and 28.8 % with S. gausapatum F28.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28",
pages = "365-354",
number = "3",
volume = "56",
doi = "10.17113/ftb.56.03.18.5348"
}

Jović, J., Buntić, A., Radovanović, N., Petrović, B.,& Mojović, L.. (2018). Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 56(3), 354-365.
https://doi.org/10.17113/ftb.56.03.18.5348

Jović J, Buntić A, Radovanović N, Petrović B, Mojović L. Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28. in Food Technology and Biotechnology. 2018;56(3):354-365.
doi:10.17113/ftb.56.03.18.5348 .

Jović, Jelena, Buntić, Aneta, Radovanović, Neda, Petrović, Bojan, Mojović, Ljiljana, "Lignin-Degrading Abilities of Novel Autochthonous Fungal Isolates Trametes hirsuta F13 and Stereum gausapatum F28" in Food Technology and Biotechnology, 56, no. 3 (2018):354-365,
https://doi.org/10.17113/ftb.56.03.18.5348 . .

TY  - JOUR
AU  - Jović, Jelena M.
AU  - Pejin, Jelena
AU  - Kocić-Tanackov, Sunčica
AU  - Mojović, Ljiljana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2886
AB  - Pretreatment is a necessary step in the process of conversion of lignocellulosic biomass to ethanol; by changing the structure of lignocellulose, enhances enzymatic hydrolysis, but often it consumes large amounts of energy and/or needs an application of expensive and toxic chemicals, which makes the process economically and ecologically unfavourable. Application of lignocellulolytic fungi (from the class Ascomycetes, Basidiomycetes and Deuteromycetes) is an attractive method for pre-treatment, environmentally friendly and does not require the investment of energy. Fungi produce a wide range of enzymes and chemicals, which, combined in a variety of ways, together successfully degrade lignocellu- lose, as well as aromatic polymers that share features with lignin. On the basis of material utilization and features of a rotten wood, they are divided in three types of wood-decay fungi: white rot, brown rot and soft rot fungi. White rot fungi are the most efficient lignin degraders in nature and, therefore, have a very important role in carbon recycling from lignified wood. This paper describes fungal mechanisms of lignocellulose degradation. They involve oxidative and hydrolytic mechanisms. Lignin peroxidase, manganese peroxidase, laccase, cellobiose dehydrogenase and enzymes able to catalyze formation of hydroxyl radicals (•OH) such as glyoxal oxidase, pyranose-2-oxidase and aryl-alcohol oxidase are responsible for oxidative processes, while cellulases and hemicellulases are involved in hydrolytic processes. Throughout the production stages, from pre-treatment to ferment-ation, the possibility of their application in the technology of bioethanol production is presented. Based on previous research, the advantages and disadvantages of biological pre-treatment are pointed out.
AB  - Predtretman predstavlja neophodan korak u procesu konverzije lignocelulozne biomase do etanola. On unapređuje enzimsku hidrolizu promenama u strukturi lignoceluloze, ali je često utrošak energije za predretman veliki i/ili se primenjuju skupe i toksične hemikalije, što proces čini ekonomski i ekološki nepogodnim. Primena lignocelulolitičkih gljiva (iz klasa Ascomycetes, Deuteromycetes i Basidiomycetes) je atraktivna metoda za predtretman, ekološki prihvatljiva i ne zahteva ulaganje energije. U ovom radu su predstavljeni mehanizmi razgradnje lignoceluloze pomoću gljiva. One proizvode širok spektar enzima i hemijskih supstanci kojima uspešno razgrađuju lignocelulozu, ali i aromatične polimere slične strukture ligninu. U ovom radu je prikazana mogućnost njihove primene u tehnologiji proizvodnje bioetanola, a navedene su i prednosti i nedostaci biološkog predtretmana.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Application of lignocellulolytic fungi for bioethanol production from renewable biomass
T1  - Primena gljiva koje razgrađuju lignocelulozu za proizvodnju bioetanola iz obnovljive biomase
EP  - 641
IS  - 6
SP  - 627
VL  - 69
DO  - 10.2298/HEMIND140916086J
ER  - 

@article{
author = "Jović, Jelena M. and Pejin, Jelena and Kocić-Tanackov, Sunčica and Mojović, Ljiljana",
year = "2015",
abstract = "Pretreatment is a necessary step in the process of conversion of lignocellulosic biomass to ethanol; by changing the structure of lignocellulose, enhances enzymatic hydrolysis, but often it consumes large amounts of energy and/or needs an application of expensive and toxic chemicals, which makes the process economically and ecologically unfavourable. Application of lignocellulolytic fungi (from the class Ascomycetes, Basidiomycetes and Deuteromycetes) is an attractive method for pre-treatment, environmentally friendly and does not require the investment of energy. Fungi produce a wide range of enzymes and chemicals, which, combined in a variety of ways, together successfully degrade lignocellu- lose, as well as aromatic polymers that share features with lignin. On the basis of material utilization and features of a rotten wood, they are divided in three types of wood-decay fungi: white rot, brown rot and soft rot fungi. White rot fungi are the most efficient lignin degraders in nature and, therefore, have a very important role in carbon recycling from lignified wood. This paper describes fungal mechanisms of lignocellulose degradation. They involve oxidative and hydrolytic mechanisms. Lignin peroxidase, manganese peroxidase, laccase, cellobiose dehydrogenase and enzymes able to catalyze formation of hydroxyl radicals (•OH) such as glyoxal oxidase, pyranose-2-oxidase and aryl-alcohol oxidase are responsible for oxidative processes, while cellulases and hemicellulases are involved in hydrolytic processes. Throughout the production stages, from pre-treatment to ferment-ation, the possibility of their application in the technology of bioethanol production is presented. Based on previous research, the advantages and disadvantages of biological pre-treatment are pointed out., Predtretman predstavlja neophodan korak u procesu konverzije lignocelulozne biomase do etanola. On unapređuje enzimsku hidrolizu promenama u strukturi lignoceluloze, ali je često utrošak energije za predretman veliki i/ili se primenjuju skupe i toksične hemikalije, što proces čini ekonomski i ekološki nepogodnim. Primena lignocelulolitičkih gljiva (iz klasa Ascomycetes, Deuteromycetes i Basidiomycetes) je atraktivna metoda za predtretman, ekološki prihvatljiva i ne zahteva ulaganje energije. U ovom radu su predstavljeni mehanizmi razgradnje lignoceluloze pomoću gljiva. One proizvode širok spektar enzima i hemijskih supstanci kojima uspešno razgrađuju lignocelulozu, ali i aromatične polimere slične strukture ligninu. U ovom radu je prikazana mogućnost njihove primene u tehnologiji proizvodnje bioetanola, a navedene su i prednosti i nedostaci biološkog predtretmana.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Application of lignocellulolytic fungi for bioethanol production from renewable biomass, Primena gljiva koje razgrađuju lignocelulozu za proizvodnju bioetanola iz obnovljive biomase",
pages = "641-627",
number = "6",
volume = "69",
doi = "10.2298/HEMIND140916086J"
}

Jović, J. M., Pejin, J., Kocić-Tanackov, S.,& Mojović, L.. (2015). Application of lignocellulolytic fungi for bioethanol production from renewable biomass. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(6), 627-641.
https://doi.org/10.2298/HEMIND140916086J

Jović JM, Pejin J, Kocić-Tanackov S, Mojović L. Application of lignocellulolytic fungi for bioethanol production from renewable biomass. in Hemijska industrija. 2015;69(6):627-641.
doi:10.2298/HEMIND140916086J .

Jović, Jelena M., Pejin, Jelena, Kocić-Tanackov, Sunčica, Mojović, Ljiljana, "Application of lignocellulolytic fungi for bioethanol production from renewable biomass" in Hemijska industrija, 69, no. 6 (2015):627-641,
https://doi.org/10.2298/HEMIND140916086J . .