TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Kocić-Tanackov, Sunčica
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/12
AB  - Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.
PB  - Springer Heidelberg, Heidelberg
T2  - Biomass Conversion and Biorefinery
T1  - Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation
EP  - 2765
IS  - 7
SP  - 2749
VL  - 12
DO  - 10.1007/s13399-020-00929-1
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Kocić-Tanackov, Sunčica and Mojović, Ljiljana",
year = "2022",
abstract = "Fungal treatment of lignocellulose is an ecologically and economically acceptable method. However, it needs improvement to increase the hydrolysis rate. A novel combination of multiple response optimization of ligninolytic activity ofTrametes hirsutaF13 and supplementation of the lignocellulosic substrate (beechwood sawdust) with sugar beet molasses stillage was employed to improve and manage the desired type of fungal ligninolytic activity leading to a significant enhancement of biomass saccharification. Obtained optimal cultivation conditions (molasses stillage concentration, 13%; substrate moisture, 63%; and temperature, 25 degrees C) provided the desired combination of laccase and manganese-dependent peroxidase activity, and maintained the activities longer and at higher values (51.5 +/- 3.5 U/L and 91 +/- 4.24 U/L, respectively) than the conditions without molasses stillage (21.5 +/- 2.12 U/L and 31.5 +/- 4.9 U/L, respectively). Enzyme hydrolysis of the substrate treated for 7 days in the presence of molasses stillage released 20.54 +/- 0.80 mg/mL fermentable sugars, and 63.58 +/- 1.47 mg/mL from 18-days treated substrate, while sugar concentrations released from the substrates pretreated without the supplementation were 16.84 +/- 0.30 mg/mL and 53.63 +/- 2.16 mg/mL, respectively. The proposed new approach of the lignocellulose supplementation with sugar beet molasses stillage and manipulation and improvement of fungal ligninolytic activity proved to be a promising solution for the enhancement of lignocellulose bioconversion.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Biomass Conversion and Biorefinery",
title = "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation",
pages = "2765-2749",
number = "7",
volume = "12",
doi = "10.1007/s13399-020-00929-1"
}

Jović, J., Hao, J., Kocić-Tanackov, S.,& Mojović, L.. (2022). Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery
Springer Heidelberg, Heidelberg., 12(7), 2749-2765.
https://doi.org/10.1007/s13399-020-00929-1

Jović J, Hao J, Kocić-Tanackov S, Mojović L. Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. in Biomass Conversion and Biorefinery. 2022;12(7):2749-2765.
doi:10.1007/s13399-020-00929-1 .

Jović, Jelena, Hao, Jian, Kocić-Tanackov, Sunčica, Mojović, Ljiljana, "Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation" in Biomass Conversion and Biorefinery, 12, no. 7 (2022):2749-2765,
https://doi.org/10.1007/s13399-020-00929-1 . .

TY  - JOUR
AU  - Wang, Qinghui
AU  - Gu, Jinjie
AU  - Shu, Lin
AU  - Jiang, Weiyan
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Shi, Jiping
AU  - Baganz, Frank
AU  - Lye, Gary J.
AU  - Xiang, Wensheng
AU  - Hao, Jian
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5109
AB  - Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.
PB  - Springer Science and Business Media B.V.
T2  - World Journal of Microbiology and Biotechnology
T1  - Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production
IS  - 5
SP  - 81
VL  - 38
DO  - 10.1007/s11274-022-03266-9
ER  - 

@article{
author = "Wang, Qinghui and Gu, Jinjie and Shu, Lin and Jiang, Weiyan and Mojović, Ljiljana and Knežević-Jugović, Zorica and Shi, Jiping and Baganz, Frank and Lye, Gary J. and Xiang, Wensheng and Hao, Jian",
year = "2022",
abstract = "Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of l-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each other in the wild-type strain. A knockout of budA blocked the 2,3-butanediol synthesis pathway and resulted in the l-valine production. The budA coded an α-acetolactate decarboxylase and catalyzed the acetoin formation from α-acetolactate. Furthermore, blocking the lactic acid synthesis by knocking out of ldhA, which is encoding a lactate dehydrogenase, improved the l-valine synthesis. 2-Ketoisovalerate is the precursor of l-valine, it is also an intermediate of the isobutanol synthesis pathway, while indole-3-pyruvate decarboxylase (ipdC) is responsible for isobutyraldehyde formation from 2-ketoisovalerate. Production of l-valine has been improved by knocking out of ipdC. On the other side, the ilvE, encoding a transaminase B, reversibly transfers one amino group from glutamate to α-ketoisovalerate. Overexpression of ilvE exhibited a distinct improvement of l-valine production. The brnQ encodes a branched-chain amino acid transporter, and l-valine production was further improved by disrupting brnQ. It is also revealed that weak acidic and aerobic conditions favor l-valine production. Based on these findings, l-valine production by metabolically engineered K. pneumonia was examined. In fed-batch fermentation, 22.4 g/L of l-valine was produced by the engineered K. pneumoniae ΔbudA-ΔldhA-ΔipdC-ΔbrnQ-ilvE after 55 h of cultivation, with a substrate conversion ratio of 0.27 mol/mol glucose.",
publisher = "Springer Science and Business Media B.V.",
journal = "World Journal of Microbiology and Biotechnology",
title = "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production",
number = "5",
pages = "81",
volume = "38",
doi = "10.1007/s11274-022-03266-9"
}

Wang, Q., Gu, J., Shu, L., Jiang, W., Mojović, L., Knežević-Jugović, Z., Shi, J., Baganz, F., Lye, G. J., Xiang, W.,& Hao, J.. (2022). Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology
Springer Science and Business Media B.V.., 38(5), 81.
https://doi.org/10.1007/s11274-022-03266-9

Wang Q, Gu J, Shu L, Jiang W, Mojović L, Knežević-Jugović Z, Shi J, Baganz F, Lye GJ, Xiang W, Hao J. Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production. in World Journal of Microbiology and Biotechnology. 2022;38(5):81.
doi:10.1007/s11274-022-03266-9 .

Wang, Qinghui, Gu, Jinjie, Shu, Lin, Jiang, Weiyan, Mojović, Ljiljana, Knežević-Jugović, Zorica, Shi, Jiping, Baganz, Frank, Lye, Gary J., Xiang, Wensheng, Hao, Jian, "Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in l-valine production" in World Journal of Microbiology and Biotechnology, 38, no. 5 (2022):81,
https://doi.org/10.1007/s11274-022-03266-9 . .

TY  - JOUR
AU  - Jović, Jelena
AU  - Hao, Jian
AU  - Mojović, Ljiljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5182
AB  - This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
EP  - 450
IS  - 4
SP  - 437
VL  - 87
DO  - 10.2298/JSC200601075J
ER  - 

@article{
author = "Jović, Jelena and Hao, Jian and Mojović, Ljiljana",
year = "2022",
abstract = "This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses stillage. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g-1 substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g-1 substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 °C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was <5 %, manganese peroxidase ≈12 %, cellulase ≈21 % and xylanase ≈16 %), which should be taken into consideration when optimizing the desired process.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage",
pages = "450-437",
number = "4",
volume = "87",
doi = "10.2298/JSC200601075J"
}

Jović, J., Hao, J.,& Mojović, L.. (2022). Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(4), 437-450.
https://doi.org/10.2298/JSC200601075J

Jović J, Hao J, Mojović L. Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage. in Journal of the Serbian Chemical Society. 2022;87(4):437-450.
doi:10.2298/JSC200601075J .

Jović, Jelena, Hao, Jian, Mojović, Ljiljana, "Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage" in Journal of the Serbian Chemical Society, 87, no. 4 (2022):437-450,
https://doi.org/10.2298/JSC200601075J . .

TY  - JOUR
AU  - Jakovetić Tanasković, Sonja
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Gazikalović, Ivana
AU  - Grbavčić, Sanja
AU  - Đorđević, Nikola
AU  - Vukašinović-Sekulić, Maja
AU  - Hao, Jian
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4848
AB  - Wheat bran is a by-product of wheat flour production used as a nutritious and economic feed ingredient, but since it possesses several anti-nutritional factors that entrap nutrients and interfere with digestion and absorption, its application is limited. The utilization of organic-rich wastes for the production of value-added products and nutritional improvement of food is a means to an end - sustainability. Here we propose addressing these challenges via fermentation by Bacillus sp. TMF-2, a strain that produces several enzymes and simultaneously, wheat bran of higher nutritional quality. Following solid-state fermentation, the soluble phenolic content of wheat bran nearly triplicated compared to the raw bran. The total proportion of polyphenols along with the antioxidant capacity and free radical scavenging rate were significantly improved, particularly the ability to reduce Fe3+ increased by 10-fold. Additionally, the activity of hydrolytic enzymes: amylase, cellulase, pectinase, mannanase, protease, and phytase increased during fermentation, whereas the level of anti-nutrient, phytic acid decreased. Among all produced enzymes, there is a special emphasis on the protease prone to degrade gluten thus resulting in gluten-free wheat bran. The results of this research could contribute to a beneficial procedure for the natural modification of wheat bran, which further augments the health benefits and utilization of wheat bran.
PB  - Academic Press
T2  - Journal of Cereal Science
T1  - Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition
SP  - 103159
VL  - 99
DO  - 10.1016/j.jcs.2020.103159
ER  - 

@article{
author = "Jakovetić Tanasković, Sonja and Šekuljica, Nataša and Jovanović, Jelena and Gazikalović, Ivana and Grbavčić, Sanja and Đorđević, Nikola and Vukašinović-Sekulić, Maja and Hao, Jian and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2021",
abstract = "Wheat bran is a by-product of wheat flour production used as a nutritious and economic feed ingredient, but since it possesses several anti-nutritional factors that entrap nutrients and interfere with digestion and absorption, its application is limited. The utilization of organic-rich wastes for the production of value-added products and nutritional improvement of food is a means to an end - sustainability. Here we propose addressing these challenges via fermentation by Bacillus sp. TMF-2, a strain that produces several enzymes and simultaneously, wheat bran of higher nutritional quality. Following solid-state fermentation, the soluble phenolic content of wheat bran nearly triplicated compared to the raw bran. The total proportion of polyphenols along with the antioxidant capacity and free radical scavenging rate were significantly improved, particularly the ability to reduce Fe3+ increased by 10-fold. Additionally, the activity of hydrolytic enzymes: amylase, cellulase, pectinase, mannanase, protease, and phytase increased during fermentation, whereas the level of anti-nutrient, phytic acid decreased. Among all produced enzymes, there is a special emphasis on the protease prone to degrade gluten thus resulting in gluten-free wheat bran. The results of this research could contribute to a beneficial procedure for the natural modification of wheat bran, which further augments the health benefits and utilization of wheat bran.",
publisher = "Academic Press",
journal = "Journal of Cereal Science",
title = "Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition",
pages = "103159",
volume = "99",
doi = "10.1016/j.jcs.2020.103159"
}

Jakovetić Tanasković, S., Šekuljica, N., Jovanović, J., Gazikalović, I., Grbavčić, S., Đorđević, N., Vukašinović-Sekulić, M., Hao, J., Luković, N.,& Knežević-Jugović, Z.. (2021). Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition. in Journal of Cereal Science
Academic Press., 99, 103159.
https://doi.org/10.1016/j.jcs.2020.103159

Jakovetić Tanasković S, Šekuljica N, Jovanović J, Gazikalović I, Grbavčić S, Đorđević N, Vukašinović-Sekulić M, Hao J, Luković N, Knežević-Jugović Z. Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition. in Journal of Cereal Science. 2021;99:103159.
doi:10.1016/j.jcs.2020.103159 .

Jakovetić Tanasković, Sonja, Šekuljica, Nataša, Jovanović, Jelena, Gazikalović, Ivana, Grbavčić, Sanja, Đorđević, Nikola, Vukašinović-Sekulić, Maja, Hao, Jian, Luković, Nevena, Knežević-Jugović, Zorica, "Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition" in Journal of Cereal Science, 99 (2021):103159,
https://doi.org/10.1016/j.jcs.2020.103159 . .

TY  - JOUR
AU  - Lu, Xiyang
AU  - Yao, Yao
AU  - Yang, Yang
AU  - Zhang, Zhongxi
AU  - Gu, Jinjie
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Baganz, Frank
AU  - Lye, Gary
AU  - Shi, Jiping
AU  - Hao, Jian
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/14
AB  - Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Ethylene glycol and glycolic acid production by wild-typeEscherichia coli
EP  - 755
IS  - 4
SP  - 744
SP  - 755
VL  - 68
DO  - 10.1002/bab.1987
ER  - 

@article{
author = "Lu, Xiyang and Yao, Yao and Yang, Yang and Zhang, Zhongxi and Gu, Jinjie and Mojović, Ljiljana and Knežević-Jugović, Zorica and Baganz, Frank and Lye, Gary and Shi, Jiping and Hao, Jian",
year = "2021",
abstract = "Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli",
pages = "755-744-755",
number = "4",
volume = "68",
doi = "10.1002/bab.1987"
}

Lu, X., Yao, Y., Yang, Y., Zhang, Z., Gu, J., Mojović, L., Knežević-Jugović, Z., Baganz, F., Lye, G., Shi, J.,& Hao, J.. (2021). Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 68(4), 744-755.
https://doi.org/10.1002/bab.1987

Lu X, Yao Y, Yang Y, Zhang Z, Gu J, Mojović L, Knežević-Jugović Z, Baganz F, Lye G, Shi J, Hao J. Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry. 2021;68(4):744-755.
doi:10.1002/bab.1987 .

Lu, Xiyang, Yao, Yao, Yang, Yang, Zhang, Zhongxi, Gu, Jinjie, Mojović, Ljiljana, Knežević-Jugović, Zorica, Baganz, Frank, Lye, Gary, Shi, Jiping, Hao, Jian, "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli" in Biotechnology and Applied Biochemistry, 68, no. 4 (2021):744-755,
https://doi.org/10.1002/bab.1987 . .

TY  - JOUR
AU  - Sun, Yuehong
AU  - Wei, Dong
AU  - Shi, Jiping
AU  - Mojović, Ljiljana
AU  - Han, Zengsheng
AU  - Hao, Jian
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2832
AB  - 2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study. During the first stage, the culture was maintained at neutral pH, favoring cell growth. During the second stage, the culture pH was switched to acidic conditions favoring 2-ketogluconic acid accumulation. Culture parameters, including switching time, dissolved oxygen levels, pH, and temperature were optimized for the fed-batch fermentation. Characteristics of glucose dehydrogenase and gluconate dehydrogenase were revealed in vitro, and the optimal pHs of the two enzymes coincided with the optimum culture pH. Under optimum conditions, a total of 186 g/l 2-ketogluconic acid was produced at 26 h, and the conversion ratio was 0.98 mol/mol. This fermentation strategy has successfully overcome the mismatch between optimum parameters required for cell growth and 2-ketogluconic acid accumulation, and this result has the highest productivity and conversion ratio of 2-ketogluconic and produced by microorganism.
PB  - Korean Soc Microbiology & Biotechnology, Seoul
T2  - Journal of Microbiology and Biotechnology
T1  - Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae
EP  - 787
IS  - 6
SP  - 781
VL  - 24
DO  - 10.4014/jmb.1401.01038
ER  - 

@article{
author = "Sun, Yuehong and Wei, Dong and Shi, Jiping and Mojović, Ljiljana and Han, Zengsheng and Hao, Jian",
year = "2014",
abstract = "2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study. During the first stage, the culture was maintained at neutral pH, favoring cell growth. During the second stage, the culture pH was switched to acidic conditions favoring 2-ketogluconic acid accumulation. Culture parameters, including switching time, dissolved oxygen levels, pH, and temperature were optimized for the fed-batch fermentation. Characteristics of glucose dehydrogenase and gluconate dehydrogenase were revealed in vitro, and the optimal pHs of the two enzymes coincided with the optimum culture pH. Under optimum conditions, a total of 186 g/l 2-ketogluconic acid was produced at 26 h, and the conversion ratio was 0.98 mol/mol. This fermentation strategy has successfully overcome the mismatch between optimum parameters required for cell growth and 2-ketogluconic acid accumulation, and this result has the highest productivity and conversion ratio of 2-ketogluconic and produced by microorganism.",
publisher = "Korean Soc Microbiology & Biotechnology, Seoul",
journal = "Journal of Microbiology and Biotechnology",
title = "Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae",
pages = "787-781",
number = "6",
volume = "24",
doi = "10.4014/jmb.1401.01038"
}

Sun, Y., Wei, D., Shi, J., Mojović, L., Han, Z.,& Hao, J.. (2014). Two-Stage Fermentation for 2-Ketogluconic Acid Production by Klebsiella pneumoniae. in Journal of Microbiology and Biotechnology
Korean Soc Microbiology & Biotechnology, Seoul., 24(6), 781-787.
https://doi.org/10.4014/jmb.1401.01038

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