@conference{
author = "Tadić, Tamara and Marković, Bojana and Nastasović, Aleksandra and Onjia, Antonije",
year = "2021",
abstract = "Molecularly imprinted polymers (MIPs) are new functional polymers with pre-designed
molecular selectivity. Synthesis of MIP is performed using a template added into a
polymer matrix. After that, the template is removed, leaving cavities and active
binding sites. A template could be a target compound, its fragment or a molecule
similar to the target molecule by its size, shape and functional groups (dummy
template). In this way, the molecularly imprinted polymers can be bonded to original
templates, as well as related template molecules. Due to their robustness, long-term
stability, high selectivity, reproducibility, and cost-effectiveness, they are suitable for a
wide range of applications such as chemical and biological sensors, solid-phase
extraction, selective carriers for various types of chromatography, artificial receptors
for drug testing, and human viral pathogens detection. Because of continuous
evolution and fast virus mutation, there is a need for technologies that would evolve
as fast. So far, MIP has been used for the detection of influenza virus, dengue virus,
and human immunodeficiency virus. Detection of different subtypes of influenza virus
(H5N1, H5N3, H1N1, H1N3, and H6N1) is obtained using a quartz sensor QCM
coated with MIP based on methacrylate and N-vinylpyrrolidone. To detect the dengue
virus, a MIP has been developed to recognize the non-structural protein NS1 found in
the virus itself. Linear 15-mer peptides derived from NS1 protein of Japanese
encephalitis are used as a template (Thr-Glu-Leu-Arg-Tir-Ser-Trp-Lis-Thr-Trp-Gli-LisAla-Lis-Met) based on dengue NS1 virus epitope mapping to antibody D2/8-1.
Dopamine is used as a functional monomer for the synthesis of MIP for HIV
detection, while a synthetic peptide with 35 amino acid residues similar to the amino
acid sequence on the glycoprotein HIV-1 gp41 is used as a template. Based on
previous research of similar viruses, this type of polymer is found to have a potential
for coronavirus detection. A specific coronavirus aptamer would be used as a
template, while the monomers would be acrylate-based., Molekulsko otisnuti polimeri (MIP) predstavljaju nove funkcionalne polimere sa
unapred dizajniranom molekulskom selektivnošću. Sinteza MIP-a se vrši korišćenjem
šablona koji se dodaje u polimernu matricu, nakon čega se uklanja ostavljajući
šupljine i aktivna mesta vezivanja. Šablon može biti ciljno jedinjenje, njegov fragment
ili molekul veličine, oblika i funkcionalnih grupa sličnih ciljnom (tzv. lažni šablon). Na
taj način molekulsko otisnuti polimeri mogu vezati kako originalne, tako i srodne
molekule šablona. Zahvaljujući robusnosti, dugoročnoj stabilnosti, visokoj
selektivnosti, obnovljivosti i ekonomičnosti pogodni su za širok spektar primene kao
što su hemijski i biološki senzori, ekstrakcija čvrste faze, selektivni nosači za različite
vrste hromatografije, veštački receptori za ispitivanje lekova, kao i za detekciju
humanih virusnih patogena. Zbog stalne evolucije i brzine mutiranja virusa javlja se
potreba za tehnologijama koje će ići u korak sa novonastalim situacijama. U
dosadašnjim istraživanjima MIP je korišćen za detekciju virusa gripa, denga virusa,
kao i virusa humane imunodeficijencije. Detekcija različitih podtipova virusa gripa
(H5N1, H5N3, H1N1, H1N3 i H6N1) ostvarena je pomoću kvarcnog senzora QCM
koji je premazan MIP-om na bazi metakrilata i N-vinilpirolidona. Za otkrivanje virusa
denge razvijen je MIP za prepoznavanje nestrukturnog proteina NS1 koji se nalazi u
samom virusu. Linearni 15-merni peptidi izvedeni iz NS1 proteina Japanskog
encefalitisa korišćeni su kao šablon (Thr-Glu-Leu-Arg-Tir-Ser-Trp-Lis-Thr-Trp-Gli-LisAla-Lis-Met) na osnovu mapiranja epitopa virusa denge NS1 prema antitelu D2/8-1.
Kao funkcionalni monomer u sintezi MIP-a za detekciju HIV virusa koristi se
dopamin, dok se kao šablon koristi sintetički peptid sa 35 aminokiselinska ostatka
sličan aminokiselinskoj sekvenci na glikoproteinu HIV-1 gp41. Na osnovu prethodnih
saznanja o sličnim virusima, ova vrsta polimera mogla bi da ima potencijalnu
primenu za detekciju korona virusa. Kao šablon koristio bi se specifični aptamer
korona virusa, a monomeri bi bili na bazi akrilata.",
publisher = "Beograd : Naučno-stručno društvo za zaštitu životne sredine Srbije Ecologica",
journal = "Book of abstracts = Knjiga apstrakata / International Scientific Conference The Impact of the COVID-19 Pandemic on the Economy and the Environment in the Era of The Fourth Industrial Revolution = Međunarodna naučna konferencija Uticaj pandemije COVID-19 na ekonomiju i životnu sredinu u eri četvrte industrijske revolucije, Belgrade, 22-24 April 2021",
title = "Application of sensors based on molecularly imprinted polymers for virus detection, Primena senzora na bazi molekulski otisnutih polimera za detekciju virusa",
pages = "43-42",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7087"
}
