TY  - JOUR
AU  - Pravilović, Radoslava
AU  - Janković, Tamara
AU  - Veljković, Milica
AU  - Todić, Branislav
AU  - Simović, Milica
AU  - Bezbradica, Dejan
AU  - Nikačević, Nikola
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6385
AB  - A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.
PB  - John Wiley and Sons Inc.
T2  - AIChE Journal
T1  - Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products
IS  - 9
SP  - e18122
VL  - 69
DO  - 10.1002/aic.18122
ER  - 

@article{
author = "Pravilović, Radoslava and Janković, Tamara and Veljković, Milica and Todić, Branislav and Simović, Milica and Bezbradica, Dejan and Nikačević, Nikola",
year = "2023",
abstract = "A new micro-kinetic model of the enzyme-catalyzed synthesis of fructo-oligosaccharides (FOS) was developed. A commercial enzyme mixture Pectinex® Ultra SP-L derived from Aspergillus aculeatus was used. A variety of initial enzyme concentrations (1–5 vol%) and sucrose concentrations (400–600 g/L) were experimentally investigated and included in kinetic modeling. Several variations of kinetic mechanisms and corresponding models have been examined. A hybrid genetic algorithm was used to predict the kinetic parameters simultaneously for all experimental data. The best fitting model has been adopted, and with an average error of 13.34%, it describes the experimental data very well. The influence of initial concentrations on the conversion of sucrose and production of FOS is being carefully investigated. It was shown that the initial sucrose concentration significantly affects the highest level of FOS concentration, but the enzyme concentration controls the time at which maximum is reached as well as the rate of FOS decomposition.",
publisher = "John Wiley and Sons Inc.",
journal = "AIChE Journal",
title = "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products",
number = "9",
pages = "e18122",
volume = "69",
doi = "10.1002/aic.18122"
}

Pravilović, R., Janković, T., Veljković, M., Todić, B., Simović, M., Bezbradica, D.,& Nikačević, N.. (2023). Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal
John Wiley and Sons Inc.., 69(9), e18122.
https://doi.org/10.1002/aic.18122

Pravilović R, Janković T, Veljković M, Todić B, Simović M, Bezbradica D, Nikačević N. Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products. in AIChE Journal. 2023;69(9):e18122.
doi:10.1002/aic.18122 .

Pravilović, Radoslava, Janković, Tamara, Veljković, Milica, Todić, Branislav, Simović, Milica, Bezbradica, Dejan, Nikačević, Nikola, "Micro-kinetic model of fructo-oligosaccharide synthesis for prebiotic products" in AIChE Journal, 69, no. 9 (2023):e18122,
https://doi.org/10.1002/aic.18122 . .