TY  - JOUR
AU  - Grbavčić, Sanja
AU  - Marković, Darka
AU  - Rajilić-Stojanović, Mirjana
AU  - Antov, Mirjana
AU  - Sciban, Marina
AU  - Karadžić, Ivanka
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2977
AB  - A lipase derived from an indigenous extremophile Pseudomonas aeruginosa strain isolated from rancid metalworking fluid was evaluated as a detergent additive. Applicability of the obtained enzyme as an additive in detergent formulations was confirmed by its implementation in the formulations of several new products differing in surfactant type and concentrations, demonstrating satisfactory performance in terms of degreasing efficiency and composition of the washing wastewater. The degreasing efficiency of different enzyme-containing detergent formulations was studied on cotton fabric samples stained with triolein and compared to that of formulations containing only surfactant. The highest efficiency of the fatty soil removal in formulations with a low content of surfactants (0.4 %) was noted in the enzyme formulation containing Lutensol(A (R)) XP-80 (degreasing efficiency  gt  80 %) and Triton(A (R)) X-100 (degreasing efficiency  gt  60 %). An attempt was then made to optimize the composition of the enzyme formulation on the basis of one or both of these surfactants using statistically planned experiments and response surface methodology (RSM). Taking into consideration the environmental aspects and the shown detergency, it appeared that rather high degreasing effects were achieved in formulations based on a low quantities of Lutensol(A (R)) XP-80 (0.4 %) at all pH values. However, pH seemed to have a notable effect since the degreasing efficiency significantly increased with increasing pH and the amount of the enzyme. Formulations having a moderate alkaline pH profile and higher amount of enzyme exhibited a high cleaning performance of fatty soil even at a low concentration of the surfactant.
PB  - Wiley, Hoboken
T2  - Journal of Surfactants and Detergents
T1  - Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain
EP  - 395
IS  - 3
SP  - 383
VL  - 18
DO  - 10.1007/s11743-015-1674-x
ER  - 

@article{
author = "Grbavčić, Sanja and Marković, Darka and Rajilić-Stojanović, Mirjana and Antov, Mirjana and Sciban, Marina and Karadžić, Ivanka and Knežević-Jugović, Zorica",
year = "2015",
abstract = "A lipase derived from an indigenous extremophile Pseudomonas aeruginosa strain isolated from rancid metalworking fluid was evaluated as a detergent additive. Applicability of the obtained enzyme as an additive in detergent formulations was confirmed by its implementation in the formulations of several new products differing in surfactant type and concentrations, demonstrating satisfactory performance in terms of degreasing efficiency and composition of the washing wastewater. The degreasing efficiency of different enzyme-containing detergent formulations was studied on cotton fabric samples stained with triolein and compared to that of formulations containing only surfactant. The highest efficiency of the fatty soil removal in formulations with a low content of surfactants (0.4 %) was noted in the enzyme formulation containing Lutensol(A (R)) XP-80 (degreasing efficiency  gt  80 %) and Triton(A (R)) X-100 (degreasing efficiency  gt  60 %). An attempt was then made to optimize the composition of the enzyme formulation on the basis of one or both of these surfactants using statistically planned experiments and response surface methodology (RSM). Taking into consideration the environmental aspects and the shown detergency, it appeared that rather high degreasing effects were achieved in formulations based on a low quantities of Lutensol(A (R)) XP-80 (0.4 %) at all pH values. However, pH seemed to have a notable effect since the degreasing efficiency significantly increased with increasing pH and the amount of the enzyme. Formulations having a moderate alkaline pH profile and higher amount of enzyme exhibited a high cleaning performance of fatty soil even at a low concentration of the surfactant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Surfactants and Detergents",
title = "Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain",
pages = "395-383",
number = "3",
volume = "18",
doi = "10.1007/s11743-015-1674-x"
}

Grbavčić, S., Marković, D., Rajilić-Stojanović, M., Antov, M., Sciban, M., Karadžić, I.,& Knežević-Jugović, Z.. (2015). Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain. in Journal of Surfactants and Detergents
Wiley, Hoboken., 18(3), 383-395.
https://doi.org/10.1007/s11743-015-1674-x

Grbavčić S, Marković D, Rajilić-Stojanović M, Antov M, Sciban M, Karadžić I, Knežević-Jugović Z. Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain. in Journal of Surfactants and Detergents. 2015;18(3):383-395.
doi:10.1007/s11743-015-1674-x .

Grbavčić, Sanja, Marković, Darka, Rajilić-Stojanović, Mirjana, Antov, Mirjana, Sciban, Marina, Karadžić, Ivanka, Knežević-Jugović, Zorica, "Development of an Environmentally Acceptable Detergent Formulation for Fatty Soils Based on the Lipase from the Indigenous Extremophile Pseudomonas aeruginosa Strain" in Journal of Surfactants and Detergents, 18, no. 3 (2015):383-395,
https://doi.org/10.1007/s11743-015-1674-x . .

TY  - JOUR
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Grbavčić, Sanja
AU  - Bezbradica, Dejan
AU  - Avramović, Nataša
AU  - Karadžić, Ivanka
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2329
AB  - Pseudomonas aeruginosa has been repeatedly reported as a powerful producer of rhamnolipid biosurfactants as well as hydrolytic enzymes. In this study, the effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. The investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween® 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration: 0.8%, Tween® 80 concentration: 0.05%, temperature: 30 °C and fermentation time: 72 h) and 11111 IU dm-3 (sunflower concentration: of 0.4%, Tween® 80 concentration: 0.05%, temperature: 30 °C and fermentation time: 120 h), respectively.
AB  - Pseudomonas aeruginosa san-ai, izolovan je iz izrazito alkalne emulzije koja je korišćena kao mazivo u industriji pri obradi metala. Sposobnost da preživi u visoko alkalnoj sredini (pH 10) učinila je ovaj mikroorganizam veoma interesantnim za istraživanje, budući da je za preživljavanje u tako ekstremnim uslovima neophodno da mikroorganizam proizvodi enzime specifičnih karakteristika. Prethodna istraživanja su pokazala da ovaj ekstremofilni mikroorganizam ekstracelularno produkuje hidrolitičke enzime, koji zbog izuzetno atraktivnih karakteristika imaju potencijal za primenu u nizu biotehnoloških postupaka. Ipak, iako je pokazano da ovaj atraktivni soj produkuje industrijski veoma interesantne biomolekule (proteaze i lipaze), produkcija ramnolipida, jedinjenja čija oblast primene svakodnevno raste, pomoću ovog soja je malo ispitana. Ramnolipidi su amfifilna jedinjenja, koja se sastoje iz hidrofilne šećerne komponente i hidrofobne komponente koju čine β-hidroksi masne kiseline. Spadaju u grupu mikrobioloških surfaktanata ili biosurfaktanata, koji bi trebalo u budućnosti da se koriste kao zamena za sintetičke surfaktante koji nisu biodegradabilni i kao takvi predstavljaju opasnost za životnu sredinu. Sve veće interesovanje za industrijsku primenu ramnolipida, dovelo je do potrebe za optimizacijom njihove proizvodnje. Cilj ovog rada bila je optimizacija produkcije ramnolipida kao i lipaze pomoću Pseudomonas aeruginosa san-ai. Ispitan je uticaj četri fermentaciona faktora: koncentracije suncokretovog ulja u intervalu: 0,2-1,0 % (w/v), Tween® 80 u intervalu: 0-0,2 % (v/v), temperature: 20­60 °C i vremena trajanja fermentacije: 48-144 h. Uticaj fermentacionih faktora na prinos navedenih metabolita ispitan je pomoću centralnog kompozitnog rotatabilnog eksperimentalnog plana, na pet nivoa vrednosti ispitivanih faktora. Analizom dobijenih regresionih koeficijenata ustanovljeno je da su vrlo izražena interaktivna dejstva nekoliko parova faktora. Kod produkcije ramnolipida, najveća je vrednost koeficijenta koji opisuje negativnu interakciju između koncentracije suncokretovog ulja i temperature, a kao bitne pokazale su se i pozitivna interakcija između koncentracije Tween® 80 i temperature, kao i negativna interakcija između koncentracija suncokretovog ulja i Tween® 80. Interesantno je da su se kod produkcije lipaze kao značajni faktori pokazali samo temperatura i vreme fermentacije. Najveći prinos ramnolipida, 138 mg dm-3, postignut je pri niskoj koncentraciji Tween® 80 (0,05 %) i visokoj koncentraciji ulja (0,8 %) na 30 °C posle 72 h, dok je najveća lipolitička aktivnost, 11111 IU dm-3, ostvarena pri istoj koncentraciji Tween® 80 (0,05 %) i istoj temperaturi od 30 °C, nešto nižoj koncentraciji suncokretovog ulja (0,4 %) i dužem vremenu fermentacije od 120 h.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: The process comparison analysis by statistical approach
T1  - Proizvodnja ramnolipida i lipaze iz Pseudomonas aeruginosa san-ai - optimizacija procesa primenom metode odzivnih površina
EP  - 685
IS  - 4
SP  - 677
VL  - 67
DO  - 10.2298/HEMIND121008114J
ER  - 

@article{
author = "Jakovetić, Sonja and Knežević-Jugović, Zorica and Grbavčić, Sanja and Bezbradica, Dejan and Avramović, Nataša and Karadžić, Ivanka",
year = "2013",
abstract = "Pseudomonas aeruginosa has been repeatedly reported as a powerful producer of rhamnolipid biosurfactants as well as hydrolytic enzymes. In this study, the effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. The investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween® 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration: 0.8%, Tween® 80 concentration: 0.05%, temperature: 30 °C and fermentation time: 72 h) and 11111 IU dm-3 (sunflower concentration: of 0.4%, Tween® 80 concentration: 0.05%, temperature: 30 °C and fermentation time: 120 h), respectively., Pseudomonas aeruginosa san-ai, izolovan je iz izrazito alkalne emulzije koja je korišćena kao mazivo u industriji pri obradi metala. Sposobnost da preživi u visoko alkalnoj sredini (pH 10) učinila je ovaj mikroorganizam veoma interesantnim za istraživanje, budući da je za preživljavanje u tako ekstremnim uslovima neophodno da mikroorganizam proizvodi enzime specifičnih karakteristika. Prethodna istraživanja su pokazala da ovaj ekstremofilni mikroorganizam ekstracelularno produkuje hidrolitičke enzime, koji zbog izuzetno atraktivnih karakteristika imaju potencijal za primenu u nizu biotehnoloških postupaka. Ipak, iako je pokazano da ovaj atraktivni soj produkuje industrijski veoma interesantne biomolekule (proteaze i lipaze), produkcija ramnolipida, jedinjenja čija oblast primene svakodnevno raste, pomoću ovog soja je malo ispitana. Ramnolipidi su amfifilna jedinjenja, koja se sastoje iz hidrofilne šećerne komponente i hidrofobne komponente koju čine β-hidroksi masne kiseline. Spadaju u grupu mikrobioloških surfaktanata ili biosurfaktanata, koji bi trebalo u budućnosti da se koriste kao zamena za sintetičke surfaktante koji nisu biodegradabilni i kao takvi predstavljaju opasnost za životnu sredinu. Sve veće interesovanje za industrijsku primenu ramnolipida, dovelo je do potrebe za optimizacijom njihove proizvodnje. Cilj ovog rada bila je optimizacija produkcije ramnolipida kao i lipaze pomoću Pseudomonas aeruginosa san-ai. Ispitan je uticaj četri fermentaciona faktora: koncentracije suncokretovog ulja u intervalu: 0,2-1,0 % (w/v), Tween® 80 u intervalu: 0-0,2 % (v/v), temperature: 20­60 °C i vremena trajanja fermentacije: 48-144 h. Uticaj fermentacionih faktora na prinos navedenih metabolita ispitan je pomoću centralnog kompozitnog rotatabilnog eksperimentalnog plana, na pet nivoa vrednosti ispitivanih faktora. Analizom dobijenih regresionih koeficijenata ustanovljeno je da su vrlo izražena interaktivna dejstva nekoliko parova faktora. Kod produkcije ramnolipida, najveća je vrednost koeficijenta koji opisuje negativnu interakciju između koncentracije suncokretovog ulja i temperature, a kao bitne pokazale su se i pozitivna interakcija između koncentracije Tween® 80 i temperature, kao i negativna interakcija između koncentracija suncokretovog ulja i Tween® 80. Interesantno je da su se kod produkcije lipaze kao značajni faktori pokazali samo temperatura i vreme fermentacije. Najveći prinos ramnolipida, 138 mg dm-3, postignut je pri niskoj koncentraciji Tween® 80 (0,05 %) i visokoj koncentraciji ulja (0,8 %) na 30 °C posle 72 h, dok je najveća lipolitička aktivnost, 11111 IU dm-3, ostvarena pri istoj koncentraciji Tween® 80 (0,05 %) i istoj temperaturi od 30 °C, nešto nižoj koncentraciji suncokretovog ulja (0,4 %) i dužem vremenu fermentacije od 120 h.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: The process comparison analysis by statistical approach, Proizvodnja ramnolipida i lipaze iz Pseudomonas aeruginosa san-ai - optimizacija procesa primenom metode odzivnih površina",
pages = "685-677",
number = "4",
volume = "67",
doi = "10.2298/HEMIND121008114J"
}

Jakovetić, S., Knežević-Jugović, Z., Grbavčić, S., Bezbradica, D., Avramović, N.,& Karadžić, I.. (2013). Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: The process comparison analysis by statistical approach. in Hemijska industrija
Association of Chemical Engineers of Serbia., 67(4), 677-685.
https://doi.org/10.2298/HEMIND121008114J

Jakovetić S, Knežević-Jugović Z, Grbavčić S, Bezbradica D, Avramović N, Karadžić I. Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: The process comparison analysis by statistical approach. in Hemijska industrija. 2013;67(4):677-685.
doi:10.2298/HEMIND121008114J .

Jakovetić, Sonja, Knežević-Jugović, Zorica, Grbavčić, Sanja, Bezbradica, Dejan, Avramović, Nataša, Karadžić, Ivanka, "Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: The process comparison analysis by statistical approach" in Hemijska industrija, 67, no. 4 (2013):677-685,
https://doi.org/10.2298/HEMIND121008114J . .

TY  - JOUR
AU  - Grbavčić, Sanja
AU  - Bezbradica, Dejan
AU  - Izrael-Zivković, Lidija
AU  - Avramović, Nataša
AU  - Milosavić, Nenad
AU  - Karadžić, Ivanka
AU  - Knežević-Jugović, Zorica
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1895
AB  - An indigenous Pseudomonas aeruginosa strain has been studied for lipase and protease activities for their potential application in detergents. Produced enzymes were investigated in order to assess their compatibility with several surfactants, oxidizing agents and commercial detergents. The crude lipase appeared to retain high activity and stability in the presence of several surfactants and oxidizing agents and it was insusceptible to proteolysis. Lutensol (R) XP80 and Triton (R) X-100 strongly activated the lipase for a long period (up to 40 and 30% against the control after 1 h) while the protease activity was enhanced by the addition of Triton (R) WR1339 and Tween (R) 80. The washing performance of the investigated surfactants was significantly improved with the addition of the crude enzyme preparation. Studies were further undertaken to improve enzymes production. The optimization of fermentation conditions led to an 8-fold increase of lipase production, while the production of protease was enhanced by 60%.
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance
EP  - 11233
IS  - 24
SP  - 11226
VL  - 102
DO  - 10.1016/j.biortech.2011.09.076
ER  - 

@article{
author = "Grbavčić, Sanja and Bezbradica, Dejan and Izrael-Zivković, Lidija and Avramović, Nataša and Milosavić, Nenad and Karadžić, Ivanka and Knežević-Jugović, Zorica",
year = "2011",
abstract = "An indigenous Pseudomonas aeruginosa strain has been studied for lipase and protease activities for their potential application in detergents. Produced enzymes were investigated in order to assess their compatibility with several surfactants, oxidizing agents and commercial detergents. The crude lipase appeared to retain high activity and stability in the presence of several surfactants and oxidizing agents and it was insusceptible to proteolysis. Lutensol (R) XP80 and Triton (R) X-100 strongly activated the lipase for a long period (up to 40 and 30% against the control after 1 h) while the protease activity was enhanced by the addition of Triton (R) WR1339 and Tween (R) 80. The washing performance of the investigated surfactants was significantly improved with the addition of the crude enzyme preparation. Studies were further undertaken to improve enzymes production. The optimization of fermentation conditions led to an 8-fold increase of lipase production, while the production of protease was enhanced by 60%.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance",
pages = "11233-11226",
number = "24",
volume = "102",
doi = "10.1016/j.biortech.2011.09.076"
}

Grbavčić, S., Bezbradica, D., Izrael-Zivković, L., Avramović, N., Milosavić, N., Karadžić, I.,& Knežević-Jugović, Z.. (2011). Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 102(24), 11226-11233.
https://doi.org/10.1016/j.biortech.2011.09.076

Grbavčić S, Bezbradica D, Izrael-Zivković L, Avramović N, Milosavić N, Karadžić I, Knežević-Jugović Z. Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance. in Bioresource Technology. 2011;102(24):11226-11233.
doi:10.1016/j.biortech.2011.09.076 .

Grbavčić, Sanja, Bezbradica, Dejan, Izrael-Zivković, Lidija, Avramović, Nataša, Milosavić, Nenad, Karadžić, Ivanka, Knežević-Jugović, Zorica, "Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives: Compatibility study with detergent ingredients and washing performance" in Bioresource Technology, 102, no. 24 (2011):11226-11233,
https://doi.org/10.1016/j.biortech.2011.09.076 . .

TY  - JOUR
AU  - Grbavčić, Sanja
AU  - Bezbradica, Dejan
AU  - Karadžić, Ivanka
AU  - Knežević-Jugović, Zorica
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1405
AB  - Enzymes produced by indigenous Pseudomonas aeruginosa strain have been subjected to research considering their potential application as detergent additives. As previously noted, lipase produced by Pseudomonas aeruginosa is highly alkaline, thermostable and solvent tolerant. Furthermore, same strain exhibits both lipase and protease activity establishing this lipase as potentially desirable component of enzyme-containing detergents. Further research was carried out to investigate insusceptibility of this lipase against coexisting native protease, several commercial surfactants, oxidizing agents and commercial detergents. Lipases and proteases remained highly active when incubated with several different surfactants and oxidizing agents under washing conditions. Moreover, presence of surfactants and oxidizing agents such as Tween® 20 and Triton® X-100 initially augment lipase and protease activity. Additionally, crude lipase preparation was insusceptible to coexisting native protease hence indicating possible storage stability. Overall, the remarkable properties of these enzymes make them potential detergent additives.
AB  - Enzimi iz ekstremofilne Pseudomonas aeruginosa vrste pokazuju visoku stabilnost u alkalnoj sredini što ih čini pogodnim za primenu u proizvodnji deterdženata. U ovom radu ispitana je aktivnost lipaza i proteaza dobijenih iz ove vrste u uslovima od značaja za industriju deterdženata. Ustanovljeno je da u realnim uslovima pranja, oba enzima zadržavaju visoku aktivnost u prisustvu različitih površinski aktivnih materija i oksidacionih agenasa. Takođe, prisustvo nekih aditiva kao što su Tween® 20, Triton® X-100, SDS i NaClO u početnoj fazi povećava lipolitičku aktivnost dok je povećanje proteolitičke aktivnosti primećeno u formulacijama koje sadrže Tween® 20 i Triton® X-100. Nadalje, sama lipaza je očuvala aktivnost u prisustvu nativnih proteaza što upućuje na zaključak da bi ovi enzimi zadržali visoku efikasnost u formulacijama deterdženata i tokom procesa pranja/čišćenja.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives
T1  - Lipaze i proteaze dobijene iz ekstremofilne Pseudomonas aeruginosa vrste kao aditivi u formulacijama deterdženata
EP  - 335
IS  - 4
SP  - 331
VL  - 63
DO  - 10.2298/HEMIND0904331G
ER  - 

@article{
author = "Grbavčić, Sanja and Bezbradica, Dejan and Karadžić, Ivanka and Knežević-Jugović, Zorica",
year = "2009",
abstract = "Enzymes produced by indigenous Pseudomonas aeruginosa strain have been subjected to research considering their potential application as detergent additives. As previously noted, lipase produced by Pseudomonas aeruginosa is highly alkaline, thermostable and solvent tolerant. Furthermore, same strain exhibits both lipase and protease activity establishing this lipase as potentially desirable component of enzyme-containing detergents. Further research was carried out to investigate insusceptibility of this lipase against coexisting native protease, several commercial surfactants, oxidizing agents and commercial detergents. Lipases and proteases remained highly active when incubated with several different surfactants and oxidizing agents under washing conditions. Moreover, presence of surfactants and oxidizing agents such as Tween® 20 and Triton® X-100 initially augment lipase and protease activity. Additionally, crude lipase preparation was insusceptible to coexisting native protease hence indicating possible storage stability. Overall, the remarkable properties of these enzymes make them potential detergent additives., Enzimi iz ekstremofilne Pseudomonas aeruginosa vrste pokazuju visoku stabilnost u alkalnoj sredini što ih čini pogodnim za primenu u proizvodnji deterdženata. U ovom radu ispitana je aktivnost lipaza i proteaza dobijenih iz ove vrste u uslovima od značaja za industriju deterdženata. Ustanovljeno je da u realnim uslovima pranja, oba enzima zadržavaju visoku aktivnost u prisustvu različitih površinski aktivnih materija i oksidacionih agenasa. Takođe, prisustvo nekih aditiva kao što su Tween® 20, Triton® X-100, SDS i NaClO u početnoj fazi povećava lipolitičku aktivnost dok je povećanje proteolitičke aktivnosti primećeno u formulacijama koje sadrže Tween® 20 i Triton® X-100. Nadalje, sama lipaza je očuvala aktivnost u prisustvu nativnih proteaza što upućuje na zaključak da bi ovi enzimi zadržali visoku efikasnost u formulacijama deterdženata i tokom procesa pranja/čišćenja.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives, Lipaze i proteaze dobijene iz ekstremofilne Pseudomonas aeruginosa vrste kao aditivi u formulacijama deterdženata",
pages = "335-331",
number = "4",
volume = "63",
doi = "10.2298/HEMIND0904331G"
}

Grbavčić, S., Bezbradica, D., Karadžić, I.,& Knežević-Jugović, Z.. (2009). Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives. in Hemijska industrija
Association of Chemical Engineers of Serbia., 63(4), 331-335.
https://doi.org/10.2298/HEMIND0904331G

Grbavčić S, Bezbradica D, Karadžić I, Knežević-Jugović Z. Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives. in Hemijska industrija. 2009;63(4):331-335.
doi:10.2298/HEMIND0904331G .

Grbavčić, Sanja, Bezbradica, Dejan, Karadžić, Ivanka, Knežević-Jugović, Zorica, "Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives" in Hemijska industrija, 63, no. 4 (2009):331-335,
https://doi.org/10.2298/HEMIND0904331G . .