TY  - JOUR
AU  - Scanlan, Pauline D.
AU  - Stensvold, Christen R.
AU  - Rajilić-Stojanović, Mirjana
AU  - Heilig, Hans G. H. J.
AU  - de Vos, Willem M.
AU  - O'Toole, Paul W.
AU  - Cotter, Paul D.
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2741
AB  - To date, the majority of research into the human gut microbiota has focused on the bacterial fraction of the community. Inevitably, this has resulted in a poor understanding of the diversity and functionality of other intestinal microorganisms in the human gut. One such nonbacterial member is the microbial eukaryote Blastocystis, which has been implicated in the aetiology of a range of different intestinal and extra-intestinal diseases. However, prevalence data from different studies are conflicting, and crucially, there is limited information on its incidence and diversity in healthy individuals. Here, we survey the prevalence, genetic diversity and temporal stability of Blastocystis in a group of healthy adults (n=105) using a sensitive PCR assay. Blastocystis was present in 56% of our sample set, which is much higher than previously reported from an industrialised county (Ireland). Moreover, a diversity of different subtypes (species) were detected, and Blastocystis was present in a subset of individuals sampled over a period of time between 6 and 10years, indicating that it is capable of long-term host colonisation. These results show that Blastocystis is a common and diverse member of the healthy gut microbiota, thereby extending our knowledge of the microbial ecology of the healthy human intestine.
PB  - Oxford Univ Press, Oxford
T2  - FEMS Microbiology Ecology
T1  - The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota
EP  - 330
IS  - 1
SP  - 326
VL  - 90
DO  - 10.1111/1574-6941.12396
ER  - 

@article{
author = "Scanlan, Pauline D. and Stensvold, Christen R. and Rajilić-Stojanović, Mirjana and Heilig, Hans G. H. J. and de Vos, Willem M. and O'Toole, Paul W. and Cotter, Paul D.",
year = "2014",
abstract = "To date, the majority of research into the human gut microbiota has focused on the bacterial fraction of the community. Inevitably, this has resulted in a poor understanding of the diversity and functionality of other intestinal microorganisms in the human gut. One such nonbacterial member is the microbial eukaryote Blastocystis, which has been implicated in the aetiology of a range of different intestinal and extra-intestinal diseases. However, prevalence data from different studies are conflicting, and crucially, there is limited information on its incidence and diversity in healthy individuals. Here, we survey the prevalence, genetic diversity and temporal stability of Blastocystis in a group of healthy adults (n=105) using a sensitive PCR assay. Blastocystis was present in 56% of our sample set, which is much higher than previously reported from an industrialised county (Ireland). Moreover, a diversity of different subtypes (species) were detected, and Blastocystis was present in a subset of individuals sampled over a period of time between 6 and 10years, indicating that it is capable of long-term host colonisation. These results show that Blastocystis is a common and diverse member of the healthy gut microbiota, thereby extending our knowledge of the microbial ecology of the healthy human intestine.",
publisher = "Oxford Univ Press, Oxford",
journal = "FEMS Microbiology Ecology",
title = "The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota",
pages = "330-326",
number = "1",
volume = "90",
doi = "10.1111/1574-6941.12396"
}

Scanlan, P. D., Stensvold, C. R., Rajilić-Stojanović, M., Heilig, H. G. H. J., de Vos, W. M., O'Toole, P. W.,& Cotter, P. D.. (2014). The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. in FEMS Microbiology Ecology
Oxford Univ Press, Oxford., 90(1), 326-330.
https://doi.org/10.1111/1574-6941.12396

Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HGHJ, de Vos WM, O'Toole PW, Cotter PD. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. in FEMS Microbiology Ecology. 2014;90(1):326-330.
doi:10.1111/1574-6941.12396 .

Scanlan, Pauline D., Stensvold, Christen R., Rajilić-Stojanović, Mirjana, Heilig, Hans G. H. J., de Vos, Willem M., O'Toole, Paul W., Cotter, Paul D., "The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota" in FEMS Microbiology Ecology, 90, no. 1 (2014):326-330,
https://doi.org/10.1111/1574-6941.12396 . .