TY  - JOUR
AU  - Madžovska-Malagurski, Ivana
AU  - Vukašinović-Sekulić, Maja
AU  - Kostić, Danijela
AU  - Lević, Steva
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3283
AB  - The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.
PB  - IOP Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Towards antimicrobial yet bioactive Cu-alginate hydrogels
IS  - 3
VL  - 11
DO  - 10.1088/1748-6041/11/3/035015
ER  - 

@article{
author = "Madžovska-Malagurski, Ivana and Vukašinović-Sekulić, Maja and Kostić, Danijela and Lević, Steva",
year = "2016",
abstract = "The simplest approach to enhance alginate hydrogel characteristics and functional properties is to replace the calcium in the process of alginate gelation with other metallic ions which are essential for living systems. Gelling of alginate with other ions and using modern encapsulation techniques can provide new delivery systems with required properties. Hence, in this study Cu-alginate hydrogels in the form of microbeads were produced by electrostatic extrusion using gelling solutions with Cu(II) concentrations in the range 13.5-270 mM and comprehensively characterized in vitro. The variation of gelling solution concentration influenced the microbead Cu(II) content, size, biomechanical properties, Cu(II) release and subsequently potential biomedical application. The formulations chosen for biomedical evaluation showed potential for antimicrobial and tissue engineering applications. Microbeads with higher Cu(II) loading (similar to 100 mu mol g(-1)) induced immediate bactericidal effects against Escherichia coli and Staphylococcus aureus. Conversely, Cu(II) release from microbeads with the Cu(II) content of similar to 60 mu mol g(-1) was slower and they were suitable for promoting and maintaining chondrogenic phenotype of bovine calf chondrocytes in 3D culture. Results of this study have shown possibilities for tuning Cu-alginate properties for potential biomedical applications such as antimicrobial wound dressings, tissue engineering scaffolds or articular cartilage implants.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Towards antimicrobial yet bioactive Cu-alginate hydrogels",
number = "3",
volume = "11",
doi = "10.1088/1748-6041/11/3/035015"
}

Madžovska-Malagurski, I., Vukašinović-Sekulić, M., Kostić, D.,& Lević, S.. (2016). Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials
IOP Publishing Ltd, Bristol., 11(3).
https://doi.org/10.1088/1748-6041/11/3/035015

Madžovska-Malagurski I, Vukašinović-Sekulić M, Kostić D, Lević S. Towards antimicrobial yet bioactive Cu-alginate hydrogels. in Biomedical Materials. 2016;11(3).
doi:10.1088/1748-6041/11/3/035015 .

Madžovska-Malagurski, Ivana, Vukašinović-Sekulić, Maja, Kostić, Danijela, Lević, Steva, "Towards antimicrobial yet bioactive Cu-alginate hydrogels" in Biomedical Materials, 11, no. 3 (2016),
https://doi.org/10.1088/1748-6041/11/3/035015 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Kostić, Danijela
AU  - Jovanović, Zeljka
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2650
AB  - In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels
EP  - 314
SP  - 305
VL  - 111
DO  - 10.1016/j.carbpol.2014.04.063
ER  - 

@article{
author = "Stojkovska, Jasmina and Kostić, Danijela and Jovanović, Zeljka and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2014",
abstract = "In this work, we present a comprehensive approach to evaluation of alginate microbeads with included silver nanoparticles (AgNPs) at the concentration range of 0.3-5 mM for potential biomedical use by combining cytotoxicity, antibacterial activity, and silver release studies. The microbeads were investigated regarding drying and rehydration showing retention of similar to 80-85% of the initial nanoparticles as determined by UV-vis and SEM analyses. Both wet and dry microbeads were shown to release AgNPs and/or ions inducing similar growth delays of Staphylococcus aureus and Escherichia coli at the total released silver concentrations of similar to 10 mu g/ml. On the other hand, these concentrations were highly toxic for bovine chondrocytes in conventional monolayer cultures while nontoxic when cultured in alginate microbeads under biomimetic conditions in 3D perfusion bioreactors. The applied approach outlined directions for further optimization studies demonstrating Ag/alginate microbeads as potentially attractive components of soft tissue implants as well as antimicrobial wound dressings.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels",
pages = "314-305",
volume = "111",
doi = "10.1016/j.carbpol.2014.04.063"
}

Stojkovska, J., Kostić, D., Jovanović, Z., Vukašinović-Sekulić, M., Mišković-Stanković, V.,& Obradović, B.. (2014). A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 111, 305-314.
https://doi.org/10.1016/j.carbpol.2014.04.063

Stojkovska J, Kostić D, Jovanović Z, Vukašinović-Sekulić M, Mišković-Stanković V, Obradović B. A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. in Carbohydrate Polymers. 2014;111:305-314.
doi:10.1016/j.carbpol.2014.04.063 .

Stojkovska, Jasmina, Kostić, Danijela, Jovanović, Zeljka, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, Obradović, Bojana, "A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels" in Carbohydrate Polymers, 111 (2014):305-314,
https://doi.org/10.1016/j.carbpol.2014.04.063 . .