TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Martin, Ivan
AU  - Obradović, Bojana
AU  - Treppo, S
AU  - Grodzinsky, AJ
AU  - Langer, R
AU  - Freed, LE
PY  - 1999
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/243
AB  - Cartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.
PB  - Wiley, Hoboken
T2  - Journal of Orthopaedic Research
T1  - Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage
EP  - 138
IS  - 1
SP  - 130
VL  - 17
DO  - 10.1002/jor.1100170119
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Martin, Ivan and Obradović, Bojana and Treppo, S and Grodzinsky, AJ and Langer, R and Freed, LE",
year = "1999",
abstract = "Cartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.",
publisher = "Wiley, Hoboken",
journal = "Journal of Orthopaedic Research",
title = "Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage",
pages = "138-130",
number = "1",
volume = "17",
doi = "10.1002/jor.1100170119"
}

Vunjak-Novaković, G., Martin, I., Obradović, B., Treppo, S., Grodzinsky, A., Langer, R.,& Freed, L.. (1999). Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. in Journal of Orthopaedic Research
Wiley, Hoboken., 17(1), 130-138.
https://doi.org/10.1002/jor.1100170119

Vunjak-Novaković G, Martin I, Obradović B, Treppo S, Grodzinsky A, Langer R, Freed L. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. in Journal of Orthopaedic Research. 1999;17(1):130-138.
doi:10.1002/jor.1100170119 .

Vunjak-Novaković, Gordana, Martin, Ivan, Obradović, Bojana, Treppo, S, Grodzinsky, AJ, Langer, R, Freed, LE, "Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage" in Journal of Orthopaedic Research, 17, no. 1 (1999):130-138,
https://doi.org/10.1002/jor.1100170119 . .