TY  - JOUR
AU  - Najmi, Ziba
AU  - Kumar, Ajay
AU  - Scalia, Alessandro C.
AU  - Cochis, Andrea
AU  - Obradović, Bojana
AU  - Grassi, Federico A.
AU  - Leigheb, Massimiliano
AU  - Lamghari, Meriem
AU  - Loinaz, Iraida
AU  - Gracia, Raquel
AU  - Rimondini, Lia
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4503
AB  - Cartilage repair still represents a challenge for clinicians and only few effective therapies are nowadays available. In fact, surgery is limited by the tissue poor self-healing capacity while the autologous transplantation is often forsaken due to the poorin vitroexpansion capacity of chondrocytes. Biomaterials science offers a unique alternative based on the replacement of the injured tissue with an artificial tissue-mimicking scaffold. However, the implantation surgical practices and the scaffold itself can be a source of bacterial infection that currently represents the first reason of implants failure due to the increasing antibiotics resistance of pathogens. So, alternative antibacterial tools to prevent infections and consequent device removal are urgently required. In this work, the role of Nisin and LL-37 peptides has been investigated as alternative to antibiotics to their antimicrobial performances for direct application at the surgical site or as doping chemicals for devices aimed at articular cartilage repair. First, peptides cytocompatibility was investigated toward human mesenchymal stem cells to determine safe concentrations; then, the broad-range antibacterial activity was verified toward the Gram-positiveStaphylococcus aureusandStaphylococcus epidermidisas well as the Gram-negativeEscherichia coliandAggregatibacter actinomycetemcomitanspathogens. The peptides selective antibacterial activity was verified by a cells-bacteria co-culture assay, while chondrogenesis was assayed to exclude any interference within the differentiation route to simulate the tissue repair. In the next phase, the experiments were repeated by moving from the cell monolayer model to 3D cartilage-like spheroids to revisit the peptides activity in a more physiologically relevant environment model. Finally, the spheroid model was applied in a perfusion bioreactor to simulate an infection in the presence of circulating peptides within a physiological environment. Results suggested that 75 mu g/ml Nisin can be considered as a very promising candidate since it was shown to be more cytocompatible and potent against the investigated bacteria than LL-37 in all the tested models.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment
VL  - 8
DO  - 10.3389/fbioe.2020.00561
ER  - 

@article{
author = "Najmi, Ziba and Kumar, Ajay and Scalia, Alessandro C. and Cochis, Andrea and Obradović, Bojana and Grassi, Federico A. and Leigheb, Massimiliano and Lamghari, Meriem and Loinaz, Iraida and Gracia, Raquel and Rimondini, Lia",
year = "2020",
abstract = "Cartilage repair still represents a challenge for clinicians and only few effective therapies are nowadays available. In fact, surgery is limited by the tissue poor self-healing capacity while the autologous transplantation is often forsaken due to the poorin vitroexpansion capacity of chondrocytes. Biomaterials science offers a unique alternative based on the replacement of the injured tissue with an artificial tissue-mimicking scaffold. However, the implantation surgical practices and the scaffold itself can be a source of bacterial infection that currently represents the first reason of implants failure due to the increasing antibiotics resistance of pathogens. So, alternative antibacterial tools to prevent infections and consequent device removal are urgently required. In this work, the role of Nisin and LL-37 peptides has been investigated as alternative to antibiotics to their antimicrobial performances for direct application at the surgical site or as doping chemicals for devices aimed at articular cartilage repair. First, peptides cytocompatibility was investigated toward human mesenchymal stem cells to determine safe concentrations; then, the broad-range antibacterial activity was verified toward the Gram-positiveStaphylococcus aureusandStaphylococcus epidermidisas well as the Gram-negativeEscherichia coliandAggregatibacter actinomycetemcomitanspathogens. The peptides selective antibacterial activity was verified by a cells-bacteria co-culture assay, while chondrogenesis was assayed to exclude any interference within the differentiation route to simulate the tissue repair. In the next phase, the experiments were repeated by moving from the cell monolayer model to 3D cartilage-like spheroids to revisit the peptides activity in a more physiologically relevant environment model. Finally, the spheroid model was applied in a perfusion bioreactor to simulate an infection in the presence of circulating peptides within a physiological environment. Results suggested that 75 mu g/ml Nisin can be considered as a very promising candidate since it was shown to be more cytocompatible and potent against the investigated bacteria than LL-37 in all the tested models.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment",
volume = "8",
doi = "10.3389/fbioe.2020.00561"
}

Najmi, Z., Kumar, A., Scalia, A. C., Cochis, A., Obradović, B., Grassi, F. A., Leigheb, M., Lamghari, M., Loinaz, I., Gracia, R.,& Rimondini, L.. (2020). Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fbioe.2020.00561

Najmi Z, Kumar A, Scalia AC, Cochis A, Obradović B, Grassi FA, Leigheb M, Lamghari M, Loinaz I, Gracia R, Rimondini L. Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment. in Frontiers in Bioengineering and Biotechnology. 2020;8.
doi:10.3389/fbioe.2020.00561 .

Najmi, Ziba, Kumar, Ajay, Scalia, Alessandro C., Cochis, Andrea, Obradović, Bojana, Grassi, Federico A., Leigheb, Massimiliano, Lamghari, Meriem, Loinaz, Iraida, Gracia, Raquel, Rimondini, Lia, "Evaluation of Nisin and LL-37 Antimicrobial Peptides as Tool to Preserve Articular Cartilage Healing in a Septic Environment" in Frontiers in Bioengineering and Biotechnology, 8 (2020),
https://doi.org/10.3389/fbioe.2020.00561 . .