TY  - JOUR
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Tsui, Gary C. P.
AU  - Tang, C. Y.
AU  - Mišković-Stanković, Vesna
AU  - Stevanović, Tatjana
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2694
AB  - Hydroxyapatite (HAP) is the most suitable biocompatible material for bone implant coatings; its brittleness, however, is a major obstacle, and the reason why research focuses on creating composites with biopolymers. Organosolv lignin (Lig) is used for the production of composite coatings, and these composites were examined in this study. Titanium substrate is a key biomedical material due to its well-known properties, but infections of the implantation site still impose a serious threat. One approach to prevent infection is to improve antimicrobial properties of the coating material. Silver doped hydroxyapatite (Ag/HAP) and HAP coatings on titanium were obtained by an electrophoretic deposition method in order to control deposited coating mass and morphology by varying applied voltage and deposition time. The effect of lignin on microstructure, morphology and thermal behavior of biocomposite coatings was investigated. The results showed that higher lignin concentrations protect the HAP lattice during sintering, improving coating stability. The corrosion stability was evaluated in simulated body fluid (SBF) at 37 degrees C. Newly formed plate-shaped carbonate-HAP was detected, indicating enhanced bioactive performance. The antimicrobial efficiency of Ag/HAP/Lig was confirmed by its higher reduction of bacteria Staphylococcus aureus TL (S. aureus TL) than of HAP/Lig coating. Cytotoxicity assay revealed that both coatings can be classified as non-toxic against healthy immunocompetent peripheral blood mononuclear cells (PBMC).
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Novel Bioactive Antimicrobial Lignin Containing Coatings on Titanium Obtained by Electrophoretic Deposition
EP  - 12322
IS  - 7
SP  - 12294
VL  - 15
DO  - 10.3390/ijms150712294
ER  - 

@article{
author = "Eraković, Sanja and Janković, Ana and Tsui, Gary C. P. and Tang, C. Y. and Mišković-Stanković, Vesna and Stevanović, Tatjana",
year = "2014",
abstract = "Hydroxyapatite (HAP) is the most suitable biocompatible material for bone implant coatings; its brittleness, however, is a major obstacle, and the reason why research focuses on creating composites with biopolymers. Organosolv lignin (Lig) is used for the production of composite coatings, and these composites were examined in this study. Titanium substrate is a key biomedical material due to its well-known properties, but infections of the implantation site still impose a serious threat. One approach to prevent infection is to improve antimicrobial properties of the coating material. Silver doped hydroxyapatite (Ag/HAP) and HAP coatings on titanium were obtained by an electrophoretic deposition method in order to control deposited coating mass and morphology by varying applied voltage and deposition time. The effect of lignin on microstructure, morphology and thermal behavior of biocomposite coatings was investigated. The results showed that higher lignin concentrations protect the HAP lattice during sintering, improving coating stability. The corrosion stability was evaluated in simulated body fluid (SBF) at 37 degrees C. Newly formed plate-shaped carbonate-HAP was detected, indicating enhanced bioactive performance. The antimicrobial efficiency of Ag/HAP/Lig was confirmed by its higher reduction of bacteria Staphylococcus aureus TL (S. aureus TL) than of HAP/Lig coating. Cytotoxicity assay revealed that both coatings can be classified as non-toxic against healthy immunocompetent peripheral blood mononuclear cells (PBMC).",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Novel Bioactive Antimicrobial Lignin Containing Coatings on Titanium Obtained by Electrophoretic Deposition",
pages = "12322-12294",
number = "7",
volume = "15",
doi = "10.3390/ijms150712294"
}

Eraković, S., Janković, A., Tsui, G. C. P., Tang, C. Y., Mišković-Stanković, V.,& Stevanović, T.. (2014). Novel Bioactive Antimicrobial Lignin Containing Coatings on Titanium Obtained by Electrophoretic Deposition. in International Journal of Molecular Sciences
MDPI, Basel., 15(7), 12294-12322.
https://doi.org/10.3390/ijms150712294

Eraković S, Janković A, Tsui GCP, Tang CY, Mišković-Stanković V, Stevanović T. Novel Bioactive Antimicrobial Lignin Containing Coatings on Titanium Obtained by Electrophoretic Deposition. in International Journal of Molecular Sciences. 2014;15(7):12294-12322.
doi:10.3390/ijms150712294 .

Eraković, Sanja, Janković, Ana, Tsui, Gary C. P., Tang, C. Y., Mišković-Stanković, Vesna, Stevanović, Tatjana, "Novel Bioactive Antimicrobial Lignin Containing Coatings on Titanium Obtained by Electrophoretic Deposition" in International Journal of Molecular Sciences, 15, no. 7 (2014):12294-12322,
https://doi.org/10.3390/ijms150712294 . .

TY  - JOUR
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Veljović, Đorđe
AU  - Palcevskis, Eriks
AU  - Mitrić, Miodrag
AU  - Stevanović, Tatjana
AU  - Janaćković, Đorđe
AU  - Mišković-Stanković, Vesna
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2509
AB  - Hydroxyapatite is the most suitable biocompatible material for bone implant coatings. However, its brittleness is a major obstacle, and that is why, recently, research focused on creating composites with various biopolymers. In this study, hydroxyapatite coatings were modified with lignin in order to attain corrosion stability and surface porosity that enables osteogenesis. Incorporating silver, well known for its antimicrobial properties, seemed the best strategy for avoiding possible infections. The silver/hydroxyapatite (Ag/HAP) and silver/hydroxyapatite/lignin (Ag/HAP/Lig) coatings were cathaphoretically deposited on titanium from ethanol suspensions, sintered at 900 degrees C in Ar, and characterized by X-ray diffraction, scanning electron microscopy, field emission scanning electron microscopy, attenuated total reflection Fourier transform infrared, and X-ray photoelectron spectroscopy. The corrosion stability of electrodeposited coatings was evaluated in vitro in Kokubo's simulated body fluid (SBF) at 37 degrees C using electrochemical impedance spectroscopy. Bioactivity was estimated by immersion in SBF to evaluate the formation of hydroxyapatite on the coating surface. A microcrystalline structure of newly formed plate-shaped carbonate-hydroxyapatite was detected after only 7 days, indicating enhanced bioactive behavior. Both coatings had good corrosion stability during a prolonged immersion time. Among the two, the Ag/HAP/Lig coating had a homogeneous surface, less roughness, and low values of contact angle.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Physical Chemistry B
T1  - Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition
EP  - 1643
IS  - 6
SP  - 1633
VL  - 117
DO  - 10.1021/jp305252a
ER  - 

@article{
author = "Eraković, Sanja and Janković, Ana and Veljović, Đorđe and Palcevskis, Eriks and Mitrić, Miodrag and Stevanović, Tatjana and Janaćković, Đorđe and Mišković-Stanković, Vesna",
year = "2013",
abstract = "Hydroxyapatite is the most suitable biocompatible material for bone implant coatings. However, its brittleness is a major obstacle, and that is why, recently, research focused on creating composites with various biopolymers. In this study, hydroxyapatite coatings were modified with lignin in order to attain corrosion stability and surface porosity that enables osteogenesis. Incorporating silver, well known for its antimicrobial properties, seemed the best strategy for avoiding possible infections. The silver/hydroxyapatite (Ag/HAP) and silver/hydroxyapatite/lignin (Ag/HAP/Lig) coatings were cathaphoretically deposited on titanium from ethanol suspensions, sintered at 900 degrees C in Ar, and characterized by X-ray diffraction, scanning electron microscopy, field emission scanning electron microscopy, attenuated total reflection Fourier transform infrared, and X-ray photoelectron spectroscopy. The corrosion stability of electrodeposited coatings was evaluated in vitro in Kokubo's simulated body fluid (SBF) at 37 degrees C using electrochemical impedance spectroscopy. Bioactivity was estimated by immersion in SBF to evaluate the formation of hydroxyapatite on the coating surface. A microcrystalline structure of newly formed plate-shaped carbonate-hydroxyapatite was detected after only 7 days, indicating enhanced bioactive behavior. Both coatings had good corrosion stability during a prolonged immersion time. Among the two, the Ag/HAP/Lig coating had a homogeneous surface, less roughness, and low values of contact angle.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Physical Chemistry B",
title = "Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition",
pages = "1643-1633",
number = "6",
volume = "117",
doi = "10.1021/jp305252a"
}

Eraković, S., Janković, A., Veljović, Đ., Palcevskis, E., Mitrić, M., Stevanović, T., Janaćković, Đ.,& Mišković-Stanković, V.. (2013). Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition. in Journal of Physical Chemistry B
Amer Chemical Soc, Washington., 117(6), 1633-1643.
https://doi.org/10.1021/jp305252a

Eraković S, Janković A, Veljović Đ, Palcevskis E, Mitrić M, Stevanović T, Janaćković Đ, Mišković-Stanković V. Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition. in Journal of Physical Chemistry B. 2013;117(6):1633-1643.
doi:10.1021/jp305252a .

Eraković, Sanja, Janković, Ana, Veljović, Đorđe, Palcevskis, Eriks, Mitrić, Miodrag, Stevanović, Tatjana, Janaćković, Đorđe, Mišković-Stanković, Vesna, "Corrosion Stability and Bioactivity in Simulated Body Fluid of Silver/Hydroxyapatite and Silver/Hydroxyapatite/Lignin Coatings on Titanium Obtained by Electrophoretic Deposition" in Journal of Physical Chemistry B, 117, no. 6 (2013):1633-1643,
https://doi.org/10.1021/jp305252a . .

TY  - JOUR
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Vukašinović-Sekulić, Maja
AU  - Stevanović, Tatjana
AU  - Mišković-Stanković, Vesna
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2447
AB  - Silver doped hydroxyapatite (HAP) [Ca9.95Ag0.05(PO4)(6)(OH)(2)] composite coatings with natural polymer organosolv lignin (Lig) were produced by electrophoretic deposition (EPD) on titanium. Coatings were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The corrosion stability of electrodeposited coatings was evaluated in vitro in Kokubo's simulated body fluid (SBF) at 37 degrees C using electrochemical impedance spectroscopy (EIS). Antimicrobial properties are directly proportional to the rate of silver ions release from the coatings, determined from inductively coupled plasma spectrometry (ICP-AES). The obtained results are in good agreement with viability of pathogenic bacteria strain Staphylococcus aureus TL in suspension, which had completely disappeared after 24 h. Composite Ag/HAP/Lig coatings were confirmed as non-toxic for healthy immunocompetent peripheral blood mononuclear cells (PBMC).
PB  - Elsevier Science Sa, Lausanne
T2  - Materials Chemistry and Physics
T1  - Investigation of silver impact on hydroxyapatite/lignin coatings electrodeposited on titanium
EP  - 530
IS  - 2-3
SP  - 521
VL  - 142
DO  - 10.1016/j.matchemphys.2013.07.047
ER  - 

@article{
author = "Eraković, Sanja and Janković, Ana and Matić, Ivana Z. and Juranić, Zorica D. and Vukašinović-Sekulić, Maja and Stevanović, Tatjana and Mišković-Stanković, Vesna",
year = "2013",
abstract = "Silver doped hydroxyapatite (HAP) [Ca9.95Ag0.05(PO4)(6)(OH)(2)] composite coatings with natural polymer organosolv lignin (Lig) were produced by electrophoretic deposition (EPD) on titanium. Coatings were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The corrosion stability of electrodeposited coatings was evaluated in vitro in Kokubo's simulated body fluid (SBF) at 37 degrees C using electrochemical impedance spectroscopy (EIS). Antimicrobial properties are directly proportional to the rate of silver ions release from the coatings, determined from inductively coupled plasma spectrometry (ICP-AES). The obtained results are in good agreement with viability of pathogenic bacteria strain Staphylococcus aureus TL in suspension, which had completely disappeared after 24 h. Composite Ag/HAP/Lig coatings were confirmed as non-toxic for healthy immunocompetent peripheral blood mononuclear cells (PBMC).",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "Investigation of silver impact on hydroxyapatite/lignin coatings electrodeposited on titanium",
pages = "530-521",
number = "2-3",
volume = "142",
doi = "10.1016/j.matchemphys.2013.07.047"
}

Eraković, S., Janković, A., Matić, I. Z., Juranić, Z. D., Vukašinović-Sekulić, M., Stevanović, T.,& Mišković-Stanković, V.. (2013). Investigation of silver impact on hydroxyapatite/lignin coatings electrodeposited on titanium. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 142(2-3), 521-530.
https://doi.org/10.1016/j.matchemphys.2013.07.047

Eraković S, Janković A, Matić IZ, Juranić ZD, Vukašinović-Sekulić M, Stevanović T, Mišković-Stanković V. Investigation of silver impact on hydroxyapatite/lignin coatings electrodeposited on titanium. in Materials Chemistry and Physics. 2013;142(2-3):521-530.
doi:10.1016/j.matchemphys.2013.07.047 .

Eraković, Sanja, Janković, Ana, Matić, Ivana Z., Juranić, Zorica D., Vukašinović-Sekulić, Maja, Stevanović, Tatjana, Mišković-Stanković, Vesna, "Investigation of silver impact on hydroxyapatite/lignin coatings electrodeposited on titanium" in Materials Chemistry and Physics, 142, no. 2-3 (2013):521-530,
https://doi.org/10.1016/j.matchemphys.2013.07.047 . .

TY  - JOUR
AU  - Eraković, Sanja
AU  - Veljović, Đorđe
AU  - Diouf, Papa Niokhor
AU  - Stevanović, Tatjana
AU  - Mitrić, Miodrag
AU  - Janaćković, Đorđe
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Mišković-Stanković, Vesna
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2181
AB  - Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material.
PB  - Elsevier Science Sa, Lausanne
T2  - Progress in Organic Coatings
T1  - The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium
EP  - 283
IS  - 4
SP  - 275
VL  - 75
DO  - 10.1016/j.porgcoat.2012.07.005
ER  - 

@article{
author = "Eraković, Sanja and Veljović, Đorđe and Diouf, Papa Niokhor and Stevanović, Tatjana and Mitrić, Miodrag and Janaćković, Đorđe and Matić, Ivana Z. and Juranić, Zorica D. and Mišković-Stanković, Vesna",
year = "2012",
abstract = "Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Progress in Organic Coatings",
title = "The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium",
pages = "283-275",
number = "4",
volume = "75",
doi = "10.1016/j.porgcoat.2012.07.005"
}

Eraković, S., Veljović, Đ., Diouf, P. N., Stevanović, T., Mitrić, M., Janaćković, Đ., Matić, I. Z., Juranić, Z. D.,& Mišković-Stanković, V.. (2012). The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium. in Progress in Organic Coatings
Elsevier Science Sa, Lausanne., 75(4), 275-283.
https://doi.org/10.1016/j.porgcoat.2012.07.005

Eraković S, Veljović Đ, Diouf PN, Stevanović T, Mitrić M, Janaćković Đ, Matić IZ, Juranić ZD, Mišković-Stanković V. The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium. in Progress in Organic Coatings. 2012;75(4):275-283.
doi:10.1016/j.porgcoat.2012.07.005 .

Eraković, Sanja, Veljović, Đorđe, Diouf, Papa Niokhor, Stevanović, Tatjana, Mitrić, Miodrag, Janaćković, Đorđe, Matić, Ivana Z., Juranić, Zorica D., Mišković-Stanković, Vesna, "The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium" in Progress in Organic Coatings, 75, no. 4 (2012):275-283,
https://doi.org/10.1016/j.porgcoat.2012.07.005 . .