TY  - BOOK
AU  - Obradović, Bojana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2010
AB  - "Cell and Tissue Engineering" introduces the principles and new approaches in cell and tissue engineering. It includes both the fundamentals and the current trends in cell and tissue engineering, in a way useful both to a novice and an expert in the field. The book is composed of 13 chapters all of which are written by the leading experts. It is organized to gradually assemble an insight in cell and tissue function starting form a molecular nano-level, extending to a cellular micro-level and finishing at the tissue macro-level. In specific, biological, physiological, biophysical, biochemical, medical, and engineering aspects are covered from the standpoint of the development of functional substitutes of biological tissues for potential clinical use. Topics in the area of cell engineering include cell membrane biophysics, structure and function of the cytoskeleton, cell-extracellular matrix interactions, and mechanotransduction. In the area of tissue engineering the focus is on the in vitro cultivation of functional tissue equivalents based on the integrated use of isolated cells, biomaterials, and bioreactors. The book also reviews novel techniques for cell and tissue imaging and characterization, some of which are described in detail such as atomic force microscopy. Finally, mathematical modeling methods are presented as valuable and indispensable tools in cell and tissue engineering. Numerous illustrations enhance the quality and ease of use of the presented material.
T2  - Cell and Tissue Engineering
T1  - Cell and tissue engineering
EP  - 275
SP  - 1
DO  - 10.1007/978-3-642-21913-9
ER  - 

@book{
author = "Obradović, Bojana",
year = "2012",
abstract = ""Cell and Tissue Engineering" introduces the principles and new approaches in cell and tissue engineering. It includes both the fundamentals and the current trends in cell and tissue engineering, in a way useful both to a novice and an expert in the field. The book is composed of 13 chapters all of which are written by the leading experts. It is organized to gradually assemble an insight in cell and tissue function starting form a molecular nano-level, extending to a cellular micro-level and finishing at the tissue macro-level. In specific, biological, physiological, biophysical, biochemical, medical, and engineering aspects are covered from the standpoint of the development of functional substitutes of biological tissues for potential clinical use. Topics in the area of cell engineering include cell membrane biophysics, structure and function of the cytoskeleton, cell-extracellular matrix interactions, and mechanotransduction. In the area of tissue engineering the focus is on the in vitro cultivation of functional tissue equivalents based on the integrated use of isolated cells, biomaterials, and bioreactors. The book also reviews novel techniques for cell and tissue imaging and characterization, some of which are described in detail such as atomic force microscopy. Finally, mathematical modeling methods are presented as valuable and indispensable tools in cell and tissue engineering. Numerous illustrations enhance the quality and ease of use of the presented material.",
journal = "Cell and Tissue Engineering",
title = "Cell and tissue engineering",
pages = "275-1",
doi = "10.1007/978-3-642-21913-9"
}

Obradović, B.. (2012). Cell and tissue engineering. in Cell and Tissue Engineering, 1-275.
https://doi.org/10.1007/978-3-642-21913-9

Obradović B. Cell and tissue engineering. in Cell and Tissue Engineering. 2012;:1-275.
doi:10.1007/978-3-642-21913-9 .

Obradović, Bojana, "Cell and tissue engineering" in Cell and Tissue Engineering (2012):1-275,
https://doi.org/10.1007/978-3-642-21913-9 . .

TY  - CHAP
AU  - Obradović, Bojana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2013
AB  - Tissue engineering (TE) is one of the new strategies aiming to address the problem of tissue failure by in vitro cultivation of functional tissue equivalents (please see the Chapter 1). Ideally, engineered tissues can serve to replace damaged or diseased tissues providing appropriate tissue composition, biosynthetic activity, full integration with the host tissue, and normal tissue function.
T2  - Cell and Tissue Engineering
T1  - Approaches to mathematical modeling of tissue engineering systems
EP  - 250
SP  - 228
VL  - 9783642219139
DO  - 10.1007/978-3-642-21913-9_12
ER  - 

@inbook{
author = "Obradović, Bojana",
year = "2012",
abstract = "Tissue engineering (TE) is one of the new strategies aiming to address the problem of tissue failure by in vitro cultivation of functional tissue equivalents (please see the Chapter 1). Ideally, engineered tissues can serve to replace damaged or diseased tissues providing appropriate tissue composition, biosynthetic activity, full integration with the host tissue, and normal tissue function.",
journal = "Cell and Tissue Engineering",
booktitle = "Approaches to mathematical modeling of tissue engineering systems",
pages = "250-228",
volume = "9783642219139",
doi = "10.1007/978-3-642-21913-9_12"
}

Obradović, B.. (2012). Approaches to mathematical modeling of tissue engineering systems. in Cell and Tissue Engineering, 9783642219139, 228-250.
https://doi.org/10.1007/978-3-642-21913-9_12

Obradović B. Approaches to mathematical modeling of tissue engineering systems. in Cell and Tissue Engineering. 2012;9783642219139:228-250.
doi:10.1007/978-3-642-21913-9_12 .

Obradović, Bojana, "Approaches to mathematical modeling of tissue engineering systems" in Cell and Tissue Engineering, 9783642219139 (2012):228-250,
https://doi.org/10.1007/978-3-642-21913-9_12 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Bugarski, Branko
AU  - Obradović, Bojana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1725
AB  - Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering
EP  - 2879
IS  - 10
SP  - 2869
VL  - 21
DO  - 10.1007/s10856-010-4135-0
ER  - 

@article{
author = "Stojkovska, Jasmina and Bugarski, Branko and Obradović, Bojana",
year = "2010",
abstract = "Alginate hydrogels in forms of discs and packed beds of microbeads (similar to 800 mu m) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 mu m/s and at sequential increments of 50 mu m displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (similar to 1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 x 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering",
pages = "2879-2869",
number = "10",
volume = "21",
doi = "10.1007/s10856-010-4135-0"
}

Stojkovska, J., Bugarski, B.,& Obradović, B.. (2010). Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 21(10), 2869-2879.
https://doi.org/10.1007/s10856-010-4135-0

Stojkovska J, Bugarski B, Obradović B. Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering. in Journal of Materials Science-Materials in Medicine. 2010;21(10):2869-2879.
doi:10.1007/s10856-010-4135-0 .

Stojkovska, Jasmina, Bugarski, Branko, Obradović, Bojana, "Evaluation of alginate hydrogels under in vivo-like bioreactor conditions for cartilage tissue engineering" in Journal of Materials Science-Materials in Medicine, 21, no. 10 (2010):2869-2879,
https://doi.org/10.1007/s10856-010-4135-0 . .

TY  - JOUR
AU  - Milanović, Jelena
AU  - Manojlović, Verica
AU  - Lević, Steva
AU  - Rajić, Nevenka
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1685
AB  - The subject of this study is the development of flavor wax formulations aimed for food and feed products. The melt dispersion technique was applied for the encapsulation of ethyl vanillin in wax microcapsules. The surface morphology of microparticles was investigated using scanning electron microscope (SEM), while the loading content was determined by HPLC measurements. This study shows that the decomposition process under heating proceeds in several steps: vanilla evaporation occurs at around 200 degrees C, while matrix degradation starts at 250 degrees C and progresses with maxima at around 360, 440 and 520 degrees C. The results indicate that carnauba wax is an attractive material for use as a matrix for encapsulation of flavours in order to improve their functionality and stability in products.
PB  - MDPI, Basel
T2  - Sensors
T1  - Microencapsulation of Flavors in Carnauba Wax
EP  - 912
IS  - 1
SP  - 901
VL  - 10
DO  - 10.3390/s100100901
ER  - 

@article{
author = "Milanović, Jelena and Manojlović, Verica and Lević, Steva and Rajić, Nevenka and Nedović, Viktor and Bugarski, Branko",
year = "2010",
abstract = "The subject of this study is the development of flavor wax formulations aimed for food and feed products. The melt dispersion technique was applied for the encapsulation of ethyl vanillin in wax microcapsules. The surface morphology of microparticles was investigated using scanning electron microscope (SEM), while the loading content was determined by HPLC measurements. This study shows that the decomposition process under heating proceeds in several steps: vanilla evaporation occurs at around 200 degrees C, while matrix degradation starts at 250 degrees C and progresses with maxima at around 360, 440 and 520 degrees C. The results indicate that carnauba wax is an attractive material for use as a matrix for encapsulation of flavours in order to improve their functionality and stability in products.",
publisher = "MDPI, Basel",
journal = "Sensors",
title = "Microencapsulation of Flavors in Carnauba Wax",
pages = "912-901",
number = "1",
volume = "10",
doi = "10.3390/s100100901"
}

Milanović, J., Manojlović, V., Lević, S., Rajić, N., Nedović, V.,& Bugarski, B.. (2010). Microencapsulation of Flavors in Carnauba Wax. in Sensors
MDPI, Basel., 10(1), 901-912.
https://doi.org/10.3390/s100100901

Milanović J, Manojlović V, Lević S, Rajić N, Nedović V, Bugarski B. Microencapsulation of Flavors in Carnauba Wax. in Sensors. 2010;10(1):901-912.
doi:10.3390/s100100901 .

Milanović, Jelena, Manojlović, Verica, Lević, Steva, Rajić, Nevenka, Nedović, Viktor, Bugarski, Branko, "Microencapsulation of Flavors in Carnauba Wax" in Sensors, 10, no. 1 (2010):901-912,
https://doi.org/10.3390/s100100901 . .

TY  - CHAP
AU  - Manojlović, Verica
AU  - Nedović, Viktor A.
AU  - Kailasapathy, Kasipathy
AU  - Jan Zuidam, Nicolaas
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6188
AB  - The history of the role of probiotics for human health is one century old and several definitions have been derived hitherto. One of them, launched by Huis in’t Veld and Havenaar (1991) defines probiotics as being “mono or mixed cultures of live microorganisms which, when applied to a man or an animal (e.g., as dried cells or as a fermented product), beneficially affect the host by improving the properties of the indigenous microflora”. Probiotics are living microorganisms which survive gastric, bile, and pancreatic secretions, attach to epithelial cells and colonize the human intestine (Del Piano et al. 2006). It is estimated that an adult human intestine contains more than 400 different bacterial species (Finegold et al. 1977) and approximately 1014 bacterial cells (which is approximately ten times the total number of eukaryotic cells in the human body). The bacterial cells can be classified into three categories, namely, beneficial, neutral or harmful, with respect to human health. Among the beneficial bacteria are Bifidobacterium and Lactobacilli. The proportion of bifidobacteria represents the third most common genus in the gastrointestinal tract, while Bacteroides predominates at 86% of the total flora in the adult gut, followed by Eubacterium. Infant-type bifidobacteria B. bifidum are replaced with adult-type bifidobacteria, B. longum and B. adolescentis. With weaning and aging, the intestinal flora profile changes. Bifidobacteria decrease, while certain kinds of harmful bacteria increase. Changes in the intestinal flora are affected not only by aging but also by extrinsic factors, for example, stress, diet, drugs, bacterial contamination and constipation. Therefore, daily consumption of probiotic products is recommended for good health and longevity. There are numerous claimed beneficial effects and therapeutic applications of probiotic bacteria in humans, such as maintenance of normal intestinal microflora, improvement of constipation, treatment of diarrhea, enhancement of the immune system, reduction of lactose-intolerance, reduction of serum cholesterol levels, anticarcinogenic activity, and improved nutritional value of foods (Kailasapathy and Chin 2000; Lourens-Hattingh and Viljoen 2001; Mattila-Sandholm et al. 2002). The mechanisms by which probiotics exert their effects are largely unknown, but may involve modifying gut pH, antagonizing pathogens through production of antimicrobial and antibacterial compounds, competing for pathogen binding, and receptor cites, as well as for available nutrients and growth factors, stimulating immunomodulatory cells, and producing lactase (Kopp-Hoolihan 2001).
PB  - Springer
T2  - Encapsulation Technologies for Food Active Ingredients and Food Processing
T1  - Encapsulation of probiotics for use in food products
EP  - 302
SP  - 269
DO  - 10.1007/978-1-4419-1008-0_10
ER  - 

@inbook{
author = "Manojlović, Verica and Nedović, Viktor A. and Kailasapathy, Kasipathy and Jan Zuidam, Nicolaas",
year = "2010",
abstract = "The history of the role of probiotics for human health is one century old and several definitions have been derived hitherto. One of them, launched by Huis in’t Veld and Havenaar (1991) defines probiotics as being “mono or mixed cultures of live microorganisms which, when applied to a man or an animal (e.g., as dried cells or as a fermented product), beneficially affect the host by improving the properties of the indigenous microflora”. Probiotics are living microorganisms which survive gastric, bile, and pancreatic secretions, attach to epithelial cells and colonize the human intestine (Del Piano et al. 2006). It is estimated that an adult human intestine contains more than 400 different bacterial species (Finegold et al. 1977) and approximately 1014 bacterial cells (which is approximately ten times the total number of eukaryotic cells in the human body). The bacterial cells can be classified into three categories, namely, beneficial, neutral or harmful, with respect to human health. Among the beneficial bacteria are Bifidobacterium and Lactobacilli. The proportion of bifidobacteria represents the third most common genus in the gastrointestinal tract, while Bacteroides predominates at 86% of the total flora in the adult gut, followed by Eubacterium. Infant-type bifidobacteria B. bifidum are replaced with adult-type bifidobacteria, B. longum and B. adolescentis. With weaning and aging, the intestinal flora profile changes. Bifidobacteria decrease, while certain kinds of harmful bacteria increase. Changes in the intestinal flora are affected not only by aging but also by extrinsic factors, for example, stress, diet, drugs, bacterial contamination and constipation. Therefore, daily consumption of probiotic products is recommended for good health and longevity. There are numerous claimed beneficial effects and therapeutic applications of probiotic bacteria in humans, such as maintenance of normal intestinal microflora, improvement of constipation, treatment of diarrhea, enhancement of the immune system, reduction of lactose-intolerance, reduction of serum cholesterol levels, anticarcinogenic activity, and improved nutritional value of foods (Kailasapathy and Chin 2000; Lourens-Hattingh and Viljoen 2001; Mattila-Sandholm et al. 2002). The mechanisms by which probiotics exert their effects are largely unknown, but may involve modifying gut pH, antagonizing pathogens through production of antimicrobial and antibacterial compounds, competing for pathogen binding, and receptor cites, as well as for available nutrients and growth factors, stimulating immunomodulatory cells, and producing lactase (Kopp-Hoolihan 2001).",
publisher = "Springer",
journal = "Encapsulation Technologies for Food Active Ingredients and Food Processing",
booktitle = "Encapsulation of probiotics for use in food products",
pages = "302-269",
doi = "10.1007/978-1-4419-1008-0_10"
}

Manojlović, V., Nedović, V. A., Kailasapathy, K.,& Jan Zuidam, N.. (2010). Encapsulation of probiotics for use in food products. in Encapsulation Technologies for Food Active Ingredients and Food Processing
Springer., 269-302.
https://doi.org/10.1007/978-1-4419-1008-0_10

Manojlović V, Nedović VA, Kailasapathy K, Jan Zuidam N. Encapsulation of probiotics for use in food products. in Encapsulation Technologies for Food Active Ingredients and Food Processing. 2010;:269-302.
doi:10.1007/978-1-4419-1008-0_10 .

Manojlović, Verica, Nedović, Viktor A., Kailasapathy, Kasipathy, Jan Zuidam, Nicolaas, "Encapsulation of probiotics for use in food products" in Encapsulation Technologies for Food Active Ingredients and Food Processing (2010):269-302,
https://doi.org/10.1007/978-1-4419-1008-0_10 . .

TY  - CHAP
AU  - Verbelen, P.J.
AU  - Nedović, Viktor
AU  - Manojlović, V.
AU  - Delvaux, F.R.
AU  - Laskošek-Čukalović, I.
AU  - Bugarski, Branko
AU  - Willaert, R.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1739
AB  - Beer production with immobilised yeast has been the subject of research for approximately 30 years but has so far found limited application in the brewing industry, due to engineering problems, unrealised cost advantages, microbial contaminations and an unbalanced beer flavor (Linko et al. 1998; Brányik et al. 2005; Willaert and Nedović 2006). The ultimate aim of this research is the production of beer of desired quality within 1-3 days. Traditional beer fermentation systems use freely suspended yeast cells to ferment wort in an unstirred batch reactor. The primary fermentation takes approximately 7 days with a subsequent secondary fermentation (maturation) of several weeks. A batch culture system employing immobilization could benefit from an increased rate of fermentation. However, it appears that in terms of increasing productivity, a continuous fermentation system with immobilization would be the best method (Verbelen et al. 2006). An important issue of the research area is whether beer can be produced by immobilised yeast in continuous culture with the same characteristic as the traditional method. In beer production, as opposed to a process such as bio-ethanol production, the goal is to achieve a particular balance of different secondary metabolites rather than the attainment of high yields of one product. Any alterations of the fermentation procedure can thus have serious implications on the flavor profile. At present, only beer maturation and alcohol-free beer production are obtained by means of commercial-scale immobilised yeast reactors, because in these processes no real yeast growth is required. Immobilised cell physiology control and fine-tuning of the flavor compounds formation during long-term fermentation processes remain the major challenges for successful application of immobilised cell technology on an industrial scale. The key factors for the implementation of this technology on an industrial level are carrier materials, immobilization technology and bioreactor design. The purpose of this chapter is to summarise and discuss the main cell immobilization methods, process requirements, available carrier materials and bioreactor designs aimed for better yeast physiology control and fine-tuning of the flavor formation during beer fermentation process. Further, it will provide an overview on the latest important breakthroughs, accomplished in understanding of the effects of immobilization on yeast physiology, metabolism and fermentation behaviour.
T2  - Encapsulation Technologies for Active Food Ingredients and Food Processing
T1  - Bioprocess intensification of beer fermentation using immobilised cells
EP  - 325
SP  - 303
DO  - 10.1007/978-1-4419-1008-0_11
ER  - 

@inbook{
author = "Verbelen, P.J. and Nedović, Viktor and Manojlović, V. and Delvaux, F.R. and Laskošek-Čukalović, I. and Bugarski, Branko and Willaert, R.",
year = "2010",
abstract = "Beer production with immobilised yeast has been the subject of research for approximately 30 years but has so far found limited application in the brewing industry, due to engineering problems, unrealised cost advantages, microbial contaminations and an unbalanced beer flavor (Linko et al. 1998; Brányik et al. 2005; Willaert and Nedović 2006). The ultimate aim of this research is the production of beer of desired quality within 1-3 days. Traditional beer fermentation systems use freely suspended yeast cells to ferment wort in an unstirred batch reactor. The primary fermentation takes approximately 7 days with a subsequent secondary fermentation (maturation) of several weeks. A batch culture system employing immobilization could benefit from an increased rate of fermentation. However, it appears that in terms of increasing productivity, a continuous fermentation system with immobilization would be the best method (Verbelen et al. 2006). An important issue of the research area is whether beer can be produced by immobilised yeast in continuous culture with the same characteristic as the traditional method. In beer production, as opposed to a process such as bio-ethanol production, the goal is to achieve a particular balance of different secondary metabolites rather than the attainment of high yields of one product. Any alterations of the fermentation procedure can thus have serious implications on the flavor profile. At present, only beer maturation and alcohol-free beer production are obtained by means of commercial-scale immobilised yeast reactors, because in these processes no real yeast growth is required. Immobilised cell physiology control and fine-tuning of the flavor compounds formation during long-term fermentation processes remain the major challenges for successful application of immobilised cell technology on an industrial scale. The key factors for the implementation of this technology on an industrial level are carrier materials, immobilization technology and bioreactor design. The purpose of this chapter is to summarise and discuss the main cell immobilization methods, process requirements, available carrier materials and bioreactor designs aimed for better yeast physiology control and fine-tuning of the flavor formation during beer fermentation process. Further, it will provide an overview on the latest important breakthroughs, accomplished in understanding of the effects of immobilization on yeast physiology, metabolism and fermentation behaviour.",
journal = "Encapsulation Technologies for Active Food Ingredients and Food Processing",
booktitle = "Bioprocess intensification of beer fermentation using immobilised cells",
pages = "325-303",
doi = "10.1007/978-1-4419-1008-0_11"
}

Verbelen, P.J., Nedović, V., Manojlović, V., Delvaux, F.R., Laskošek-Čukalović, I., Bugarski, B.,& Willaert, R.. (2010). Bioprocess intensification of beer fermentation using immobilised cells. in Encapsulation Technologies for Active Food Ingredients and Food Processing, 303-325.
https://doi.org/10.1007/978-1-4419-1008-0_11

Verbelen P, Nedović V, Manojlović V, Delvaux F, Laskošek-Čukalović I, Bugarski B, Willaert R. Bioprocess intensification of beer fermentation using immobilised cells. in Encapsulation Technologies for Active Food Ingredients and Food Processing. 2010;:303-325.
doi:10.1007/978-1-4419-1008-0_11 .

Verbelen, P.J., Nedović, Viktor, Manojlović, V., Delvaux, F.R., Laskošek-Čukalović, I., Bugarski, Branko, Willaert, R., "Bioprocess intensification of beer fermentation using immobilised cells" in Encapsulation Technologies for Active Food Ingredients and Food Processing (2010):303-325,
https://doi.org/10.1007/978-1-4419-1008-0_11 . .

TY  - JOUR
AU  - Mitrović, Dragana D.
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1623
AB  - Alginate hydrogels are widely used in biomedicine due to alginate availability, hydrophilic nature, biocompatibility and biodegradability. Alginate microbeads are particularly attractive for applications in pharmacy and regenerative medicine due to high surface to volume ratio, low mass transfer limitations and simple implantation by injection. Aim of this work was to investigate possibilities for controlled degradation of alginate microbeads in cell culture medium (Dulbecco's modified Eagle's medium) with Na-citrate added in small concentrations (0.05-0.5 mM). Alginate microbeads (1.5% w/w, 800 μm in diameter) were produced by electrostatic droplet extrusion and evaluated over a period of 10 days regarding appearance, kinetics and degree of swelling as well as biomechanical properties determined in a novel bioreactor with mechanical stimulation under in vivo-like conditions in articular cartilage (10% strain, 337.5 μm/s compression rate). In the citrate concentration range investigated, microbeads initially swelled reaching an equilibrium value (~150-170% with respect to the initial mass), upon which they appeared stable for a certain period of time (1 to over 7 days) followed by bead bursting and degradation. This degradation process indicated that Na+ from the solution initially replaced Ca2+ bound mainly to COO- groups in polymannuronate sequences inducing electrostatic repulsion of polymer chains and, consequently, swelling of the beads. Citrate ions assisted in this process by forming insoluble calcium citrate. Thus, the specific rate of the bead swelling increased with the increase in citrate concentration approaching a maximal value of ~0.34 d-1. In the last phase, the beads burst into pieces, which slowly continued to degrade by replacement of Ca2+ bonded to polyguluronate blocks in the egg-box structure. Compression moduli for packed beds of control, freshly produced microbeads, and microbeads swelled at the equilibrium degree after 3 days of staying in 0.2 mM Na-citrate solution were 136.6±2.8 and 30.8±1.3 kPa, respectively. By day 7 in this solution, the beads still appearing structurally intact, further lost their mechanical strength due to continued polymer chain relaxation so that the compression modulus was 20.7 to 22.6 kPa owed almost solely to undegraded polyguluronate parts. Results of these studies are important from a fundamental standpoint for determination of structure and degradation mechanisms of alginate hydrogels but also from a practical point of view for optimization of hydrogel properties and behavior for potential applications in controlled drug release as well as in tissue engineering.
AB  - U ovom radu je ispitivana mogućnost kontrolisane degradacije mikročestica kalcijum-alginata (1,5 mas.%, 800 μm prečnik) u medijumu za kulture ćelija uz male koncentracije natrijum-citrata (0,05-0,5 mM). Određeni su kinetika i stepen bubrenja čestica, i njihove mehaničke karakteristike u bioreaktoru sa mehaničkom stimulacijom. Rezultati su ukazali na mehanizam razgradnje po kome Na+ prvo zamenjuju Ca2+ vezane za manuronske ostatke, dovodeći do bubrenja čestica, a zatim u drugoj fazi zamenjuju i Ca2+ čvršće vezane za guluronske ostatke što dovodi do pucanja čestica. Proces bubrenja je bio limitiran brzinom ispravljanja polimernih lanaca, a specifična brzina je pri povećanju koncentracije citrata težila maksimalnoj vrednosti od ~0,34 d-1. Dobijeni rezultati su značajni za potencijalnu primenu alginatnih hidrogelova za kontrolisano otpuštanje terapeutika kao i u inženjerstvu tkiva.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Controlled swelling and degradation studies of alginate microbeads in dilute natrium citrate solutions
T1  - Ispitivanje mogućnosti kontrolisanog bubrenja i razgradnje alginatnih mikročestica u razblaženim rastvorima natrijum-citrata
EP  - 263
IS  - 4
SP  - 253
VL  - 64
DO  - 10.2298/HEMIND100302038M
ER  - 

@article{
author = "Mitrović, Dragana D. and Stojkovska, Jasmina and Obradović, Bojana",
year = "2010",
abstract = "Alginate hydrogels are widely used in biomedicine due to alginate availability, hydrophilic nature, biocompatibility and biodegradability. Alginate microbeads are particularly attractive for applications in pharmacy and regenerative medicine due to high surface to volume ratio, low mass transfer limitations and simple implantation by injection. Aim of this work was to investigate possibilities for controlled degradation of alginate microbeads in cell culture medium (Dulbecco's modified Eagle's medium) with Na-citrate added in small concentrations (0.05-0.5 mM). Alginate microbeads (1.5% w/w, 800 μm in diameter) were produced by electrostatic droplet extrusion and evaluated over a period of 10 days regarding appearance, kinetics and degree of swelling as well as biomechanical properties determined in a novel bioreactor with mechanical stimulation under in vivo-like conditions in articular cartilage (10% strain, 337.5 μm/s compression rate). In the citrate concentration range investigated, microbeads initially swelled reaching an equilibrium value (~150-170% with respect to the initial mass), upon which they appeared stable for a certain period of time (1 to over 7 days) followed by bead bursting and degradation. This degradation process indicated that Na+ from the solution initially replaced Ca2+ bound mainly to COO- groups in polymannuronate sequences inducing electrostatic repulsion of polymer chains and, consequently, swelling of the beads. Citrate ions assisted in this process by forming insoluble calcium citrate. Thus, the specific rate of the bead swelling increased with the increase in citrate concentration approaching a maximal value of ~0.34 d-1. In the last phase, the beads burst into pieces, which slowly continued to degrade by replacement of Ca2+ bonded to polyguluronate blocks in the egg-box structure. Compression moduli for packed beds of control, freshly produced microbeads, and microbeads swelled at the equilibrium degree after 3 days of staying in 0.2 mM Na-citrate solution were 136.6±2.8 and 30.8±1.3 kPa, respectively. By day 7 in this solution, the beads still appearing structurally intact, further lost their mechanical strength due to continued polymer chain relaxation so that the compression modulus was 20.7 to 22.6 kPa owed almost solely to undegraded polyguluronate parts. Results of these studies are important from a fundamental standpoint for determination of structure and degradation mechanisms of alginate hydrogels but also from a practical point of view for optimization of hydrogel properties and behavior for potential applications in controlled drug release as well as in tissue engineering., U ovom radu je ispitivana mogućnost kontrolisane degradacije mikročestica kalcijum-alginata (1,5 mas.%, 800 μm prečnik) u medijumu za kulture ćelija uz male koncentracije natrijum-citrata (0,05-0,5 mM). Određeni su kinetika i stepen bubrenja čestica, i njihove mehaničke karakteristike u bioreaktoru sa mehaničkom stimulacijom. Rezultati su ukazali na mehanizam razgradnje po kome Na+ prvo zamenjuju Ca2+ vezane za manuronske ostatke, dovodeći do bubrenja čestica, a zatim u drugoj fazi zamenjuju i Ca2+ čvršće vezane za guluronske ostatke što dovodi do pucanja čestica. Proces bubrenja je bio limitiran brzinom ispravljanja polimernih lanaca, a specifična brzina je pri povećanju koncentracije citrata težila maksimalnoj vrednosti od ~0,34 d-1. Dobijeni rezultati su značajni za potencijalnu primenu alginatnih hidrogelova za kontrolisano otpuštanje terapeutika kao i u inženjerstvu tkiva.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Controlled swelling and degradation studies of alginate microbeads in dilute natrium citrate solutions, Ispitivanje mogućnosti kontrolisanog bubrenja i razgradnje alginatnih mikročestica u razblaženim rastvorima natrijum-citrata",
pages = "263-253",
number = "4",
volume = "64",
doi = "10.2298/HEMIND100302038M"
}

Mitrović, D. D., Stojkovska, J.,& Obradović, B.. (2010). Controlled swelling and degradation studies of alginate microbeads in dilute natrium citrate solutions. in Hemijska industrija
Association of Chemical Engineers of Serbia., 64(4), 253-263.
https://doi.org/10.2298/HEMIND100302038M

Mitrović DD, Stojkovska J, Obradović B. Controlled swelling and degradation studies of alginate microbeads in dilute natrium citrate solutions. in Hemijska industrija. 2010;64(4):253-263.
doi:10.2298/HEMIND100302038M .

Mitrović, Dragana D., Stojkovska, Jasmina, Obradović, Bojana, "Controlled swelling and degradation studies of alginate microbeads in dilute natrium citrate solutions" in Hemijska industrija, 64, no. 4 (2010):253-263,
https://doi.org/10.2298/HEMIND100302038M . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko
AU  - Plavšić, Milenko B.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1706
AB  - The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.
PB  - Springer, New York
T2  - European Biophysics Journal with Biophysics Letters
T1  - Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions
EP  - 800
IS  - 5
SP  - 789
VL  - 39
DO  - 10.1007/s00249-009-0554-6
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Ilić, Vesna Lj. and Bugarski, Branko and Plavšić, Milenko B.",
year = "2010",
abstract = "The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.",
publisher = "Springer, New York",
journal = "European Biophysics Journal with Biophysics Letters",
title = "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions",
pages = "800-789",
number = "5",
volume = "39",
doi = "10.1007/s00249-009-0554-6"
}

Pajić-Lijaković, I., Ilić, V. Lj., Bugarski, B.,& Plavšić, M. B.. (2010). Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters
Springer, New York., 39(5), 789-800.
https://doi.org/10.1007/s00249-009-0554-6

Pajić-Lijaković I, Ilić VL, Bugarski B, Plavšić MB. Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters. 2010;39(5):789-800.
doi:10.1007/s00249-009-0554-6 .

Pajić-Lijaković, Ivana, Ilić, Vesna Lj., Bugarski, Branko, Plavšić, Milenko B., "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions" in European Biophysics Journal with Biophysics Letters, 39, no. 5 (2010):789-800,
https://doi.org/10.1007/s00249-009-0554-6 . .

TY  - JOUR
AU  - Nikolaev, N. I.
AU  - Obradović, Bojana
AU  - Versteeg, H. K.
AU  - Lemon, G.
AU  - Williams, D.J.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1711
AB  - In this work a new phenomenological model of growth of cartilage tissue cultured in a rotating bioreactor is developed. It represents an advancement of a previously derived model of deposition of glycosaminoglycan (GAG) in engineered cartilage by (i) introduction of physiological mechanisms of proteoglycan accumulation in the extracellular matrix (ECM) as well as by correlating (ii) local cell densities and (iii) tissue growth to the ECM composition. In particular, previously established predictions and correlations of local oxygen concentrations and GAG synthesis rates are extended to distinguish cell secreted proteoglycan monomers free to diffuse in cell surroundings and outside from the engineered construct, from large aggrecan molecules, which are constrained within the ECM and practically immovable. The model includes kinetics of aggregation, that is, transformation of mobile GAG species into immobile aggregates as well as maintenance of the normal ECM composition after the physiological GAG concentration is reached by incorporation of a product inhibition term. The model also includes mechanisms of the temporal evolution of cell density distributions and tissue growth under in vitro conditions. After a short initial proliferation phase the total cell number in the construct remains constant, but the local cell distribution is leveled out by GAG accumulation and repulsion due to negative molecular charges. Furthermore, strong repulsive forces result in expansion of the local tissue elements observed macroscopically as tissue growth (i.e., construct enlargement). The model is validated by comparison with experimental data of (i) GAG distribution and leakage, (ii) spatial-temporal distributions of cells, and (iii) tissue growth reported in previous works. Validation of the model predictive capability-against a selection of measured data that were not used to construct the model-suggests that the model successfully describes the interplay of several simultaneous processes carried out during in vitro cartilage tissue regeneration and indicates that this approach could also be attractive for application in other tissue engineering systems. Biotechnol. Bioeng. 2010;105: 842-853.
PB  - John Wiley & Sons Inc, Hoboken
T2  - Biotechnology and Bioengineering
T1  - A Validated Model of GAG Deposition, Cell Distribution, and Growth of Tissue Engineered Cartilage Cultured in a Rotating Bioreactor
EP  - 853
IS  - 4
SP  - 842
VL  - 105
DO  - 10.1002/bit.22581
ER  - 

@article{
author = "Nikolaev, N. I. and Obradović, Bojana and Versteeg, H. K. and Lemon, G. and Williams, D.J.",
year = "2010",
abstract = "In this work a new phenomenological model of growth of cartilage tissue cultured in a rotating bioreactor is developed. It represents an advancement of a previously derived model of deposition of glycosaminoglycan (GAG) in engineered cartilage by (i) introduction of physiological mechanisms of proteoglycan accumulation in the extracellular matrix (ECM) as well as by correlating (ii) local cell densities and (iii) tissue growth to the ECM composition. In particular, previously established predictions and correlations of local oxygen concentrations and GAG synthesis rates are extended to distinguish cell secreted proteoglycan monomers free to diffuse in cell surroundings and outside from the engineered construct, from large aggrecan molecules, which are constrained within the ECM and practically immovable. The model includes kinetics of aggregation, that is, transformation of mobile GAG species into immobile aggregates as well as maintenance of the normal ECM composition after the physiological GAG concentration is reached by incorporation of a product inhibition term. The model also includes mechanisms of the temporal evolution of cell density distributions and tissue growth under in vitro conditions. After a short initial proliferation phase the total cell number in the construct remains constant, but the local cell distribution is leveled out by GAG accumulation and repulsion due to negative molecular charges. Furthermore, strong repulsive forces result in expansion of the local tissue elements observed macroscopically as tissue growth (i.e., construct enlargement). The model is validated by comparison with experimental data of (i) GAG distribution and leakage, (ii) spatial-temporal distributions of cells, and (iii) tissue growth reported in previous works. Validation of the model predictive capability-against a selection of measured data that were not used to construct the model-suggests that the model successfully describes the interplay of several simultaneous processes carried out during in vitro cartilage tissue regeneration and indicates that this approach could also be attractive for application in other tissue engineering systems. Biotechnol. Bioeng. 2010;105: 842-853.",
publisher = "John Wiley & Sons Inc, Hoboken",
journal = "Biotechnology and Bioengineering",
title = "A Validated Model of GAG Deposition, Cell Distribution, and Growth of Tissue Engineered Cartilage Cultured in a Rotating Bioreactor",
pages = "853-842",
number = "4",
volume = "105",
doi = "10.1002/bit.22581"
}

Nikolaev, N. I., Obradović, B., Versteeg, H. K., Lemon, G.,& Williams, D.J.. (2010). A Validated Model of GAG Deposition, Cell Distribution, and Growth of Tissue Engineered Cartilage Cultured in a Rotating Bioreactor. in Biotechnology and Bioengineering
John Wiley & Sons Inc, Hoboken., 105(4), 842-853.
https://doi.org/10.1002/bit.22581

Nikolaev NI, Obradović B, Versteeg HK, Lemon G, Williams D. A Validated Model of GAG Deposition, Cell Distribution, and Growth of Tissue Engineered Cartilage Cultured in a Rotating Bioreactor. in Biotechnology and Bioengineering. 2010;105(4):842-853.
doi:10.1002/bit.22581 .

Nikolaev, N. I., Obradović, Bojana, Versteeg, H. K., Lemon, G., Williams, D.J., "A Validated Model of GAG Deposition, Cell Distribution, and Growth of Tissue Engineered Cartilage Cultured in a Rotating Bioreactor" in Biotechnology and Bioengineering, 105, no. 4 (2010):842-853,
https://doi.org/10.1002/bit.22581 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Radisić, Milica
AU  - Vunjak-Novaković, Gordana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1542
AB  - Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.
T2  - NATO Science for Peace and Security Series A: Chemistry and Biology
T1  - Biomimetic Approaches to Design of Tissue Engineering Bioreactors
EP  - 129
SP  - 115
DO  - 10.1007/978-90-481-8790-4-7
ER  - 

@article{
author = "Obradović, Bojana and Radisić, Milica and Vunjak-Novaković, Gordana",
year = "2010",
abstract = "Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.",
journal = "NATO Science for Peace and Security Series A: Chemistry and Biology",
title = "Biomimetic Approaches to Design of Tissue Engineering Bioreactors",
pages = "129-115",
doi = "10.1007/978-90-481-8790-4-7"
}

Obradović, B., Radisić, M.,& Vunjak-Novaković, G.. (2010). Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology, 115-129.
https://doi.org/10.1007/978-90-481-8790-4-7

Obradović B, Radisić M, Vunjak-Novaković G. Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology. 2010;:115-129.
doi:10.1007/978-90-481-8790-4-7 .

Obradović, Bojana, Radisić, Milica, Vunjak-Novaković, Gordana, "Biomimetic Approaches to Design of Tissue Engineering Bioreactors" in NATO Science for Peace and Security Series A: Chemistry and Biology (2010):115-129,
https://doi.org/10.1007/978-90-481-8790-4-7 . .

TY  - JOUR
AU  - Petrović, Miloš
AU  - Mitraković, Dragan
AU  - Bugarski, Branko
AU  - Vonwil, Daniel
AU  - Martin, Ivan
AU  - Obradović, Bojana
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1453
AB  - The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - A novel bioreactor with mechanical stimulation for skeletal tissue engineering
EP  - 44
IS  - 1
SP  - 41
VL  - 15
DO  - 10.2298/CICEQ0901041P
ER  - 

@article{
author = "Petrović, Miloš and Mitraković, Dragan and Bugarski, Branko and Vonwil, Daniel and Martin, Ivan and Obradović, Bojana",
year = "2009",
abstract = "The provision of mechanical stimulation is believed to be necessary for the functional assembly of skeletal tissues, which are normally exposed to a variety of biomechanical signals in vivo. In this paper, we present a development and validation of a novel bioreactor aimed for skeletal tissue engineering that provides dynamic compression and perfusion of cultivated tissues. Dynamic compression can be applied at frequencies up to 67.5 Hz and displacements down to 5 µm thus suitable for the simulation of physiological conditions in a native cartilage tissue (0.1-1 Hz, 5-10 % strain). The bioreactor also includes a load sensor that was calibrated so to measure average loads imposed on tissue samples. Regimes of the mechanical stimulation and acquisition of load sensor outputs are directed by an automatic control system using applications developed within the LabView platform. In addition, perfusion of tissue samples at physiological velocities (10-100 µm/s) provides efficient mass transfer, as well as the possibilities to expose the cells to hydrodynamic shear and simulate the conditions in a native bone tissue. Thus, the novel bioreactor is suited for studies of the effects of different biomechanical signals on in vitro regeneration of skeletal tissues, as well as for the studies of newly formulated biomaterials and cell biomaterial interactions under in vivo-like settings.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "A novel bioreactor with mechanical stimulation for skeletal tissue engineering",
pages = "44-41",
number = "1",
volume = "15",
doi = "10.2298/CICEQ0901041P"
}

Petrović, M., Mitraković, D., Bugarski, B., Vonwil, D., Martin, I.,& Obradović, B.. (2009). A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 15(1), 41-44.
https://doi.org/10.2298/CICEQ0901041P

Petrović M, Mitraković D, Bugarski B, Vonwil D, Martin I, Obradović B. A novel bioreactor with mechanical stimulation for skeletal tissue engineering. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(1):41-44.
doi:10.2298/CICEQ0901041P .

Petrović, Miloš, Mitraković, Dragan, Bugarski, Branko, Vonwil, Daniel, Martin, Ivan, Obradović, Bojana, "A novel bioreactor with mechanical stimulation for skeletal tissue engineering" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 1 (2009):41-44,
https://doi.org/10.2298/CICEQ0901041P . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Vucurović, Vesna
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1363
AB  - The possibilities of improving ethanol fermentation of enzymatically obtained corn semolina hydrolyzates with alginate-immobilized yeast Saccharomyces cerevisiae var. ellipsoideus by medium supplementation with mineral salts as sources of magnesium, zinc, calcium and copper ions, and vitamins (pantothenate, thiamine, pyridoxine, biotin and inositol), separately or as combined mixtures, have been investigated. Among all tested minerals, alone or combined, the most efficient in improving ethanol productivity during fermentation of corn semolina hydrolyzates was a mixture of magnesium and zinc salts: MgSO4 (2 g/L) and ZnSO4 (0.3 g/L). Positive effects were also obtained with the addition of copper ions (CuCl2, 1 mg/L) or calcium ions (CaCl2, 40 mg/L). Among vitamins, the most effective was Ca-pantothenate (1 g/L), which caused an increase in the fermentation efficiency for approx. 8 %, compared to the control sample. Based on these results, an effective mixture of vitamins and minerals consisting Of MgSO4 (2 g/L), ZnSO4 (0.3 g/L), CuCl2 (1 mg/L), Ca-pantothenate (1 g/L) and inositol (1 g/L) was arranged for the supplementation of the medium based on corn semolina hydrolyzates. The supplementation with this Mixture provided an increase of the fermentation efficiency for 20 % compared to the control sample, without supplementation.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation
EP  - 89
IS  - 1
SP  - 83
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1363
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Vucurović, Vesna",
year = "2009",
abstract = "The possibilities of improving ethanol fermentation of enzymatically obtained corn semolina hydrolyzates with alginate-immobilized yeast Saccharomyces cerevisiae var. ellipsoideus by medium supplementation with mineral salts as sources of magnesium, zinc, calcium and copper ions, and vitamins (pantothenate, thiamine, pyridoxine, biotin and inositol), separately or as combined mixtures, have been investigated. Among all tested minerals, alone or combined, the most efficient in improving ethanol productivity during fermentation of corn semolina hydrolyzates was a mixture of magnesium and zinc salts: MgSO4 (2 g/L) and ZnSO4 (0.3 g/L). Positive effects were also obtained with the addition of copper ions (CuCl2, 1 mg/L) or calcium ions (CaCl2, 40 mg/L). Among vitamins, the most effective was Ca-pantothenate (1 g/L), which caused an increase in the fermentation efficiency for approx. 8 %, compared to the control sample. Based on these results, an effective mixture of vitamins and minerals consisting Of MgSO4 (2 g/L), ZnSO4 (0.3 g/L), CuCl2 (1 mg/L), Ca-pantothenate (1 g/L) and inositol (1 g/L) was arranged for the supplementation of the medium based on corn semolina hydrolyzates. The supplementation with this Mixture provided an increase of the fermentation efficiency for 20 % compared to the control sample, without supplementation.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation",
pages = "89-83",
number = "1",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1363"
}

Nikolić, S., Mojović, L., Pejin, D., Rakin, M.,& Vucurović, V.. (2009). Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation. in Food Technology and Biotechnology
University of Zagreb., 47(1), 83-89.
https://hdl.handle.net/21.15107/rcub_technorep_1363

Nikolić S, Mojović L, Pejin D, Rakin M, Vucurović V. Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation. in Food Technology and Biotechnology. 2009;47(1):83-89.
https://hdl.handle.net/21.15107/rcub_technorep_1363 .

Nikolić, Svetlana, Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Vucurović, Vesna, "Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation" in Food Technology and Biotechnology, 47, no. 1 (2009):83-89,
https://hdl.handle.net/21.15107/rcub_technorep_1363 .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Plavšić, Milenko B.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1221
AB  - Aim. A phase-field mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead yeast cell volume fraction profile within the microbead after reaching the equilibrium state for cells (150 h), as well as, total yeast cell volume fraction per microbead and microbead volume as functions time. Microbead with growing yeast cells is treated as a two-phase system. one phase represents the cell agglomerates, while the other is the alginate hydrogel matrices. The interactions between phases are simulated using the Langevin class, non-conservative phasefield model based on the reduction of the modeling resolution. The model considered the growth of small domains of one phase (cell agglomerates) as nucleation. Methods. Total yeast cell volume fraction in the beads was estimated by using Thoma counting chamber after dissolution of beads. Local cell volume fraction per microbead layers is calculated from experimentally determined surface fraction of cells for various microbead cross sections by image analysis. Microbead volume is estimated by measuring the microbead diameter. Diameters of microbeads were measured using the optical microscope equipped with a micrometric device. Results. The proposed model offered the only one model parameter, which represents the specific measure of microenvironmental restrictive action to the cell growth dynamics. The optimal value of this model parameter is obtained by comparison analysis between experimental data and model predictions. Conclusion. Besides giving useful insights into the dynamics of restrictive cell growth within the Ca-alginate microbead, the model can be used as a tool to design/optimize the performance of microbead and studying the microenvironmental restrictive mechanism action of the cell growth.
T2  - Minerva Biotecnologica
T1  - Modeling of microenvironmetal restricted yeast cell growth within Ca-alginate microbead
EP  - 102
IS  - 2
SP  - 99
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1221
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Plavšić, Milenko B. and Nedović, Viktor and Bugarski, Branko",
year = "2008",
abstract = "Aim. A phase-field mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead yeast cell volume fraction profile within the microbead after reaching the equilibrium state for cells (150 h), as well as, total yeast cell volume fraction per microbead and microbead volume as functions time. Microbead with growing yeast cells is treated as a two-phase system. one phase represents the cell agglomerates, while the other is the alginate hydrogel matrices. The interactions between phases are simulated using the Langevin class, non-conservative phasefield model based on the reduction of the modeling resolution. The model considered the growth of small domains of one phase (cell agglomerates) as nucleation. Methods. Total yeast cell volume fraction in the beads was estimated by using Thoma counting chamber after dissolution of beads. Local cell volume fraction per microbead layers is calculated from experimentally determined surface fraction of cells for various microbead cross sections by image analysis. Microbead volume is estimated by measuring the microbead diameter. Diameters of microbeads were measured using the optical microscope equipped with a micrometric device. Results. The proposed model offered the only one model parameter, which represents the specific measure of microenvironmental restrictive action to the cell growth dynamics. The optimal value of this model parameter is obtained by comparison analysis between experimental data and model predictions. Conclusion. Besides giving useful insights into the dynamics of restrictive cell growth within the Ca-alginate microbead, the model can be used as a tool to design/optimize the performance of microbead and studying the microenvironmental restrictive mechanism action of the cell growth.",
journal = "Minerva Biotecnologica",
title = "Modeling of microenvironmetal restricted yeast cell growth within Ca-alginate microbead",
pages = "102-99",
number = "2",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1221"
}

Pajić-Lijaković, I., Plavšić, M. B., Nedović, V.,& Bugarski, B.. (2008). Modeling of microenvironmetal restricted yeast cell growth within Ca-alginate microbead. in Minerva Biotecnologica, 20(2), 99-102.
https://hdl.handle.net/21.15107/rcub_technorep_1221

Pajić-Lijaković I, Plavšić MB, Nedović V, Bugarski B. Modeling of microenvironmetal restricted yeast cell growth within Ca-alginate microbead. in Minerva Biotecnologica. 2008;20(2):99-102.
https://hdl.handle.net/21.15107/rcub_technorep_1221 .

Pajić-Lijaković, Ivana, Plavšić, Milenko B., Nedović, Viktor, Bugarski, Branko, "Modeling of microenvironmetal restricted yeast cell growth within Ca-alginate microbead" in Minerva Biotecnologica, 20, no. 2 (2008):99-102,
https://hdl.handle.net/21.15107/rcub_technorep_1221 .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Osmokrović, Andrea
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1018
AB  - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
PB  - 8th Conference of the Yugoslav Materials Research Society
T2  - Materials Science Forum
T1  - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
EP  - 422
SP  - 417
VL  - 555
DO  - 10.4028/0-87849-441-3.417
ER  - 

@article{
author = "Obradović, Bojana and Osmokrović, Andrea and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2007",
abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.",
publisher = "8th Conference of the Yugoslav Materials Research Society",
journal = "Materials Science Forum",
title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies",
pages = "422-417",
volume = "555",
doi = "10.4028/0-87849-441-3.417"
}

Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum
8th Conference of the Yugoslav Materials Research Society., 555, 417-422.
https://doi.org/10.4028/0-87849-441-3.417

Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum. 2007;555:417-422.
doi:10.4028/0-87849-441-3.417 .

Obradović, Bojana, Osmokrović, Andrea, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Materials Science Forum, 555 (2007):417-422,
https://doi.org/10.4028/0-87849-441-3.417 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Diana
AU  - Plavšić, Milenko B.
AU  - Bugarski, Branko
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1088
AB  - A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.
PB  - Elsevier Sci Ltd, Oxford
T2  - Process Biochemistry
T1  - Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule
EP  - 174
IS  - 2
SP  - 167
VL  - 42
DO  - 10.1016/j.procbio.2006.07.023
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Bugarski, Diana and Plavšić, Milenko B. and Bugarski, Branko",
year = "2007",
abstract = "A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule",
pages = "174-167",
number = "2",
volume = "42",
doi = "10.1016/j.procbio.2006.07.023"
}

Pajić-Lijaković, I., Bugarski, D., Plavšić, M. B.,& Bugarski, B.. (2007). Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 42(2), 167-174.
https://doi.org/10.1016/j.procbio.2006.07.023

Pajić-Lijaković I, Bugarski D, Plavšić MB, Bugarski B. Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry. 2007;42(2):167-174.
doi:10.1016/j.procbio.2006.07.023 .

Pajić-Lijaković, Ivana, Bugarski, Diana, Plavšić, Milenko B., Bugarski, Branko, "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule" in Process Biochemistry, 42, no. 2 (2007):167-174,
https://doi.org/10.1016/j.procbio.2006.07.023 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Plavšić, Milenko B.
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1091
AB  - A mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead cell concentration profile, after reached the equilibrium state, as well as, total yeast cell concentration per microbed and microbead volume as function of time. Relatively uniform cell concentration in the carrier matrix indicated that no internal nutrient diffusion limitations, but microenvironmental restriction, affected dominantly the dynamics of cell growth. Also interesting phenomenon of very different rates of cell number growth during cultivation is observed. After some critical time, the growth rate of cell colonies decreased drastically, but than suddenly increased again under all other experimental condition been the same. It is interpreted as disintegration of gel network and opening new free space for growth of cell clusters. These complex phenomena are modeled using the thermodynamical, free energy formalism. The particular form of free energy functional is proposed to describe various kinds of interactions, which affected the dynamics of cell growth and cause pseudo-phase transition of hydrogel. The good agreement of experimentally obtained data and model predictions are obtained. In that way the model provides both, the quantitative tools for further technological optimization of the process and deeper insight into dynamics of cell growth mechanism.
PB  - Elsevier, Amsterdam
T2  - Journal of Biotechnology
T1  - Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics
EP  - 452
IS  - 3
SP  - 446
VL  - 129
DO  - 10.1016/j.jbiotec.2007.01.017
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Plavšić, Milenko B. and Bugarski, Branko and Nedović, Viktor",
year = "2007",
abstract = "A mathematical model was formulated to describe yeast cell growth within the Ca-alginate microbead during air-lift bioreactor cultivation. Model development was based on experimentally obtained data for the intra-bead cell concentration profile, after reached the equilibrium state, as well as, total yeast cell concentration per microbed and microbead volume as function of time. Relatively uniform cell concentration in the carrier matrix indicated that no internal nutrient diffusion limitations, but microenvironmental restriction, affected dominantly the dynamics of cell growth. Also interesting phenomenon of very different rates of cell number growth during cultivation is observed. After some critical time, the growth rate of cell colonies decreased drastically, but than suddenly increased again under all other experimental condition been the same. It is interpreted as disintegration of gel network and opening new free space for growth of cell clusters. These complex phenomena are modeled using the thermodynamical, free energy formalism. The particular form of free energy functional is proposed to describe various kinds of interactions, which affected the dynamics of cell growth and cause pseudo-phase transition of hydrogel. The good agreement of experimentally obtained data and model predictions are obtained. In that way the model provides both, the quantitative tools for further technological optimization of the process and deeper insight into dynamics of cell growth mechanism.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Biotechnology",
title = "Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics",
pages = "452-446",
number = "3",
volume = "129",
doi = "10.1016/j.jbiotec.2007.01.017"
}

Pajić-Lijaković, I., Plavšić, M. B., Bugarski, B.,& Nedović, V.. (2007). Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics. in Journal of Biotechnology
Elsevier, Amsterdam., 129(3), 446-452.
https://doi.org/10.1016/j.jbiotec.2007.01.017

Pajić-Lijaković I, Plavšić MB, Bugarski B, Nedović V. Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics. in Journal of Biotechnology. 2007;129(3):446-452.
doi:10.1016/j.jbiotec.2007.01.017 .

Pajić-Lijaković, Ivana, Plavšić, Milenko B., Bugarski, Branko, Nedović, Viktor, "Ca-alginate hydrogel mechanical transformations - The influence on yeast cell growth dynamics" in Journal of Biotechnology, 129, no. 3 (2007):446-452,
https://doi.org/10.1016/j.jbiotec.2007.01.017 . .

TY  - JOUR
AU  - Bezbradica, Dejan
AU  - Obradović, Bojana
AU  - Leskošek-Čukalović, Ida
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1132
AB  - In this study, the potential of application of non-aggressive LentiKat (R) technique for brewer's yeast immobilization on polyvinyl alcohol was assessed. High cell loads of about 109 cells/ml were achieved by this procedure and immobilization procedure had no adverse effect on cell viability. The stability and activity of obtained immobilized biocatalyst was tested in the growth studies and fermentations. Immobilized cells exhibited high fermentation activity in both, laboratory and pilot-scale fermentations. In three successive gas-lift reactor fermentations the apparent attenuation of around 80% was reached after only 2 days, indicating good potential of immobilized cells for development of continuous primary beer fermentation. LentiKat (R) particles showed high mechanical and fermentative stability, since they endured 30 days of operating time during 6-month period without significant change of cell activity, particle shape and particle size.
PB  - Elsevier Sci Ltd, Oxford
T2  - Process Biochemistry
T1  - Immobilization of yeast cells in PVA particles for beer fermentation
EP  - 1351
IS  - 9
SP  - 1348
VL  - 42
DO  - 10.1016/j.procbio.2007.04.009
ER  - 

@article{
author = "Bezbradica, Dejan and Obradović, Bojana and Leskošek-Čukalović, Ida and Bugarski, Branko and Nedović, Viktor",
year = "2007",
abstract = "In this study, the potential of application of non-aggressive LentiKat (R) technique for brewer's yeast immobilization on polyvinyl alcohol was assessed. High cell loads of about 109 cells/ml were achieved by this procedure and immobilization procedure had no adverse effect on cell viability. The stability and activity of obtained immobilized biocatalyst was tested in the growth studies and fermentations. Immobilized cells exhibited high fermentation activity in both, laboratory and pilot-scale fermentations. In three successive gas-lift reactor fermentations the apparent attenuation of around 80% was reached after only 2 days, indicating good potential of immobilized cells for development of continuous primary beer fermentation. LentiKat (R) particles showed high mechanical and fermentative stability, since they endured 30 days of operating time during 6-month period without significant change of cell activity, particle shape and particle size.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Immobilization of yeast cells in PVA particles for beer fermentation",
pages = "1351-1348",
number = "9",
volume = "42",
doi = "10.1016/j.procbio.2007.04.009"
}

Bezbradica, D., Obradović, B., Leskošek-Čukalović, I., Bugarski, B.,& Nedović, V.. (2007). Immobilization of yeast cells in PVA particles for beer fermentation. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 42(9), 1348-1351.
https://doi.org/10.1016/j.procbio.2007.04.009

Bezbradica D, Obradović B, Leskošek-Čukalović I, Bugarski B, Nedović V. Immobilization of yeast cells in PVA particles for beer fermentation. in Process Biochemistry. 2007;42(9):1348-1351.
doi:10.1016/j.procbio.2007.04.009 .

Bezbradica, Dejan, Obradović, Bojana, Leskošek-Čukalović, Ida, Bugarski, Branko, Nedović, Viktor, "Immobilization of yeast cells in PVA particles for beer fermentation" in Process Biochemistry, 42, no. 9 (2007):1348-1351,
https://doi.org/10.1016/j.procbio.2007.04.009 . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Obradović, Bojana
AU  - Bugarski, Diana
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 2006
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/897
AB  - Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering.
AB  - Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.
PB  - University of Belgrade - Faculty of Mechanical Engineering, Belgrade
T2  - FME Transactions
T1  - Development of a packed bed bioreactor for cartilage tissue engineering
T1  - Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice
EP  - 70
IS  - 2
SP  - 65
VL  - 34
UR  - https://hdl.handle.net/21.15107/rcub_technorep_897
ER  - 

@article{
author = "Osmokrović, Andrea and Obradović, Bojana and Bugarski, Diana and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "2006",
abstract = "Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering., Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.",
publisher = "University of Belgrade - Faculty of Mechanical Engineering, Belgrade",
journal = "FME Transactions",
title = "Development of a packed bed bioreactor for cartilage tissue engineering, Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice",
pages = "70-65",
number = "2",
volume = "34",
url = "https://hdl.handle.net/21.15107/rcub_technorep_897"
}

Osmokrović, A., Obradović, B., Bugarski, D., Bugarski, B.,& Vunjak-Novaković, G.. (2006). Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions
University of Belgrade - Faculty of Mechanical Engineering, Belgrade., 34(2), 65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897

Osmokrović A, Obradović B, Bugarski D, Bugarski B, Vunjak-Novaković G. Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions. 2006;34(2):65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897 .

Osmokrović, Andrea, Obradović, Bojana, Bugarski, Diana, Bugarski, Branko, Vunjak-Novaković, Gordana, "Development of a packed bed bioreactor for cartilage tissue engineering" in FME Transactions, 34, no. 2 (2006):65-70,
https://hdl.handle.net/21.15107/rcub_technorep_897 .