TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2170
AB  - The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid
EP  - 340
IS  - 1-2
SP  - 332
VL  - 436
DO  - 10.1016/j.ijpharm.2012.06.050
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid",
pages = "340-332",
number = "1-2",
volume = "436",
doi = "10.1016/j.ijpharm.2012.06.050"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics
Elsevier Science Bv, Amsterdam., 436(1-2), 332-340.
https://doi.org/10.1016/j.ijpharm.2012.06.050

Milašinović N, Knežević-Jugović Z, Milosavljević N, Filipović J, Kalagasidis Krušić M. Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics. 2012;436(1-2):332-340.
doi:10.1016/j.ijpharm.2012.06.050 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid" in International Journal of Pharmaceutics, 436, no. 1-2 (2012):332-340,
https://doi.org/10.1016/j.ijpharm.2012.06.050 . .