TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Milanović, Slađan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Miletić, Maja
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7411
AB  - The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients
IS  - 6
SP  - 3138
VL  - 25
DO  - 10.3390/ijms25063138
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Milanović, Slađan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Miletić, Maja and Bugarski, Branko and Ilić, Vesna",
year = "2024",
abstract = "The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients",
number = "6",
pages = "3138",
volume = "25",
doi = "10.3390/ijms25063138"
}

Đukić, T., Drvenica, I., Kovačić, M., Milanović, S., Majerič, D., Šefik-Bukilica, M., Miletić, M., Bugarski, B.,& Ilić, V.. (2024). Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences
MDPI., 25(6), 3138.
https://doi.org/10.3390/ijms25063138

Đukić T, Drvenica I, Kovačić M, Milanović S, Majerič D, Šefik-Bukilica M, Miletić M, Bugarski B, Ilić V. Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences. 2024;25(6):3138.
doi:10.3390/ijms25063138 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Milanović, Slađan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Miletić, Maja, Bugarski, Branko, Ilić, Vesna, "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients" in International Journal of Molecular Sciences, 25, no. 6 (2024):3138,
https://doi.org/10.3390/ijms25063138 . .

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6470
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Academic Press Inc.
T2  - Analytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Academic Press Inc.",
journal = "Analytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}

Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Academic Press Inc.., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194

Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .

TY  - JOUR
AU  - Stančić, Ana Z.
AU  - Drvenica, Ivana T.
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Branko M.
AU  - Bugarski, Diana S.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5339
AB  - Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
PB  - MDPI
T2  - Processes
T1  - Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
IS  - 1
SP  - 32
VL  - 10
DO  - 10.3390/pr10010032
ER  - 

@article{
author = "Stančić, Ana Z. and Drvenica, Ivana T. and Ilić, Vesna Lj. and Bugarski, Branko M. and Bugarski, Diana S.",
year = "2022",
abstract = "Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.",
publisher = "MDPI",
journal = "Processes",
title = "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin",
number = "1",
pages = "32",
volume = "10",
doi = "10.3390/pr10010032"
}

Stančić, A. Z., Drvenica, I. T., Ilić, V. Lj., Bugarski, B. M.,& Bugarski, D. S.. (2022). Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes
MDPI., 10(1), 32.
https://doi.org/10.3390/pr10010032

Stančić AZ, Drvenica IT, Ilić VL, Bugarski BM, Bugarski DS. Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes. 2022;10(1):32.
doi:10.3390/pr10010032 .

Stančić, Ana Z., Drvenica, Ivana T., Ilić, Vesna Lj., Bugarski, Branko M., Bugarski, Diana S., "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin" in Processes, 10, no. 1 (2022):32,
https://doi.org/10.3390/pr10010032 . .

TY  - JOUR
AU  - Vajić, Una-Jovana
AU  - Živković, Jelena
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5561
AB  - The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
EP  - 128
IS  - 1
SP  - 119
VL  - 41
DO  - 10.20450/mjcce.2022.2238
ER  - 

@article{
author = "Vajić, Una-Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology",
pages = "128-119",
number = "1",
volume = "41",
doi = "10.20450/mjcce.2022.2238"
}

Vajić, U., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 41(1), 119-128.
https://doi.org/10.20450/mjcce.2022.2238

Vajić U, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128.
doi:10.20450/mjcce.2022.2238 .

Vajić, Una-Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128,
https://doi.org/10.20450/mjcce.2022.2238 . .

TY  - JOUR
AU  - Nikodijević, Slavomir
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Kosanović, Dejana
AU  - Đukić, Tamara
AU  - Djordjević, Brižita
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5235
AB  - Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
PB  - John Wiley and Sons Inc
T2  - Scandinavian Journal of Immunology
T1  - Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans
DO  - 10.1111/sji.13223
ER  - 

@article{
author = "Nikodijević, Slavomir and Blagojević, Veljko and Ćuruvija, Ivana and Kosanović, Dejana and Đukić, Tamara and Djordjević, Brižita and Ilić, Vesna and Minić, Rajna",
year = "2022",
abstract = "Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.",
publisher = "John Wiley and Sons Inc",
journal = "Scandinavian Journal of Immunology",
title = "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans",
doi = "10.1111/sji.13223"
}

Nikodijević, S., Blagojević, V., Ćuruvija, I., Kosanović, D., Đukić, T., Djordjević, B., Ilić, V.,& Minić, R.. (2022). Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology
John Wiley and Sons Inc..
https://doi.org/10.1111/sji.13223

Nikodijević S, Blagojević V, Ćuruvija I, Kosanović D, Đukić T, Djordjević B, Ilić V, Minić R. Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology. 2022;.
doi:10.1111/sji.13223 .

Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Djordjević, Brižita, Ilić, Vesna, Minić, Rajna, "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans" in Scandinavian Journal of Immunology (2022),
https://doi.org/10.1111/sji.13223 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj.
AU  - Bugarski, Diana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4441
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna Lj. and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V. Lj.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić VL, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna Lj., Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .